Restriction map for pF1KB9479
 (Linear) (Six-base) MAPSORT of: KIBB9479.seq  Check: 4335  from: 1  to: 2193



KIBB9479.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:39  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1926    2193 
   Size:       1926     267
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     871    1162    2049    2113    2193 
   Size:        871     291     887      64      80

  Fragments arranged by size: 

                 887     871     291      80      64
 
AflIII A'CryG_T

Cuts at:      0     587    2193 
   Size:        587    1606
 
AleI CACnn'nnGTG

Cuts at:      0     328     457    2193 
   Size:        328     129    1736

  Fragments arranged by size: 

                1736     328     129
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1121    1153    2193 
   Size:       1121      32    1040

  Fragments arranged by size: 

                1121    1040      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      19     100     360     387     658     894     999    1020 
   Size:         19      81     260      27     271     236     105      21

Cuts at:   1020    1233    1317    1326    1842    2193 
   Size:        213      84       9     516     351

  Fragments arranged by size: 

                 516     351     271     260     236     213     105      84
                  81      27      21      19       9
 
ApaI G_GGCC'C

Cuts at:      0      76     606     671    1008    1045    1612    2150    2193 
   Size:         76     530      65     337      37     567     538      43

  Fragments arranged by size: 

                 567     538     530     337      76      65      43      37
            
 
ApoI r'AATT_y

Cuts at:      0    1401    2193 
   Size:       1401     792
 
AvaI C'yCGr_G

Cuts at:      0     706    2193 
   Size:        706    1487
 
BanI G'GyrC_C

Cuts at:      0     244     519    1379    1749    2193 
   Size:        244     275     860     370     444

  Fragments arranged by size: 

                 860     444     370     275     244
 
BanII G_rGCy'C

Cuts at:      0      76     111     376     606     671     948     978    1008 
   Size:         76      35     265     230      65     277      30      30

Cuts at:   1008    1045    1612    2134    2150    2193 
   Size:         37     567     522      16      43

  Fragments arranged by size: 

                 567     522     277     265     230      76      65      43
                  37      35      30      30      16
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     799    2193 
   Size:        799    1394
 
BbvCI CC'TCA_GC

Cuts at:      0     509    1166    2193 
   Size:        509     657    1027

  Fragments arranged by size: 

                1027     657     509
 
BglI GCCn_nnn'nGGC

Cuts at:      0     115     912    1372    2193 
   Size:        115     797     460     821

  Fragments arranged by size: 

                 821     797     460     115
 
BlpI GC'TnA_GC

Cuts at:      0    1289    1460    2193 
   Size:       1289     171     733

  Fragments arranged by size: 

                1289     733     171
 
Bme1580I G_kGCm'C

Cuts at:      0      76     247     524     606     671    1008    1045    1612 
   Size:         76     171     277      82      65     337      37     567

Cuts at:   1612    1752    1785    2150    2162    2193 
   Size:        140      33     365      12      31

  Fragments arranged by size: 

                 567     365     337     277     171     140      82      76
                  65      37      33      31      12
 
BmgBI CAC'GTC

Cuts at:      0     459    2193 
   Size:        459    1734
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      79     674     760    1344    1521    1819    2193 
   Size:         79     595      86     584     177     298     374

  Fragments arranged by size: 

                 595     584     374     298     177      86      79
 
BmtI G_CTAG'C

Cuts at:      0    1822    2193 
   Size:       1822     371
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1863    2193 
   Size:       1863     330
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     420     997    1000    1042    1180    1255    1348    1673 
   Size:        420     577       3      42     138      75      93     325

Cuts at:   1673    2008    2193 
   Size:        335     185

  Fragments arranged by size: 

                 577     420     335     325     185     138      93      75
                  42       3
 
Bpu10I CC'TnA_GC

Cuts at:      0     509     745    1166    1764    2193 
   Size:        509     236     421     598     429

  Fragments arranged by size: 

                 598     509     429     421     236
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     302     313    1359    1442    2193 
   Size:        302      11    1046      83     751

  Fragments arranged by size: 

                1046     751     302      83      11
 
BsaWI w'CCGG_w

Cuts at:      0    1451    2193 
   Size:       1451     742
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     115     145     281     311    2193 
   Size:        115      30     136      30    1882

  Fragments arranged by size: 

                1882     136     115      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     241     436     622     934     937    1254    1588    1638 
   Size:        241     195     186     312       3     317     334      50

Cuts at:   1638    2017    2193 
   Size:        379     176

  Fragments arranged by size: 

                 379     334     317     312     241     195     186     176
                  50       3
 
BseYI C'CCAG_C

Cuts at:      0      29     157     544     570     787     860     932    1212 
   Size:         29     128     387      26     217      73      72     280

Cuts at:   1212    1269    1293    1885    2193 
   Size:         57      24     592     308

  Fragments arranged by size: 

                 592     387     308     280     217     128      73      72
                  57      29      26      24
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     578    2193 
   Size:        578    1615
 
BsiHKAI G_wGCw'C

Cuts at:      0    1466    2193 
   Size:       1466     727
 
BsmI GAATG_Cn'

Cuts at:      0     215     933    2193 
   Size:        215     718    1260

  Fragments arranged by size: 

                1260     718     215
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     474    2193 
   Size:        474    1719
 
Bsp1286I G_dGCh'C

Cuts at:      0      76     111     247     376     524     606     671     948 
   Size:         76      35     136     129     148      82      65     277

Cuts at:    948     978    1008    1045    1466    1612    1752    1785    2134 
   Size:         30      30      37     421     146     140      33     349

Cuts at:   2134    2150    2162    2193 
   Size:         16      12      31

  Fragments arranged by size: 

                 421     349     277     148     146     140     136     129
                  82      76      65      37      35      33      31      30
                  30      16      12
 
BspHI T'CATG_A

Cuts at:      0    1950    2193 
   Size:       1950     243
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     320     681    1926    2193 
   Size:        320     361    1245     267

  Fragments arranged by size: 

                1245     361     320     267
 
BsrBI CCG'CTC

Cuts at:      0     687    2193 
   Size:        687    1506
 
BsrFI r'CCGG_y

Cuts at:      0     499    2193 
   Size:        499    1694
 
BsrGI T'GTAC_A

Cuts at:      0    1221    2193 
   Size:       1221     972
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     999    2193 
   Size:        999    1194
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1348    1651    2077    2193 
   Size:       1348     303     426     116

  Fragments arranged by size: 

                1348     426     303     116
 
BstYI r'GATC_y

Cuts at:      0    1230    2193 
   Size:       1230     963
 
BtgI C'CryG_G

Cuts at:      0     164     326     515     671    1260    2193 
   Size:        164     162     189     156     589     933

  Fragments arranged by size: 

                 933     589     189     164     162     156
 
BtsI GCAGTG_nn'

Cuts at:      0     447    2193 
   Size:        447    1746
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1657    1692    2193 
   Size:       1657      35     501

  Fragments arranged by size: 

                1657     501      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    1084    2193 
   Size:       1084    1109
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     298    1351    2193 
   Size:        298    1053     842

  Fragments arranged by size: 

                1053     842     298
 
EaeI y'GGCC_r

Cuts at:      0     824    1491    2193 
   Size:        824     667     702

  Fragments arranged by size: 

                 824     702     667
 
EarI CTCTTCn'nnn_

Cuts at:      0     190     640     875    2092    2193 
   Size:        190     450     235    1217     101

  Fragments arranged by size: 

                1217     450     235     190     101
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     420     871     997    1000    1042    1162    1180    1255 
   Size:        420     451     126       3      42     120      18      75

Cuts at:   1255    1348    1673    2008    2049    2113    2193 
   Size:         93     325     335      41      64      80

  Fragments arranged by size: 

                 451     420     335     325     126     120      93      80
                  75      64      42      41      18       3
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     284    2193 
   Size:        284    1909
 
EcoO109I rG'GnC_Cy

Cuts at:      0     117     357     602     603     627     693     762    1041 
   Size:        117     240     245       1      24      66      69     279

Cuts at:   1041    1108    1266    1323    1344    1608    1794    2146    2193 
   Size:         67     158      57      21     264     186     352      47

  Fragments arranged by size: 

                 352     279     264     245     240     186     158     117
                  69      67      66      57      47      24      21       1
            
 
EcoRI G'AATT_C

Cuts at:      0    1401    2193 
   Size:       1401     792
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1410    2193 
   Size:       1410     783
 
FseI GG_CCGG'CC

Cuts at:      0     503    2193 
   Size:        503    1690
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      82     114    2107    2139    2193 
   Size:         82      32    1993      32      54

  Fragments arranged by size: 

                1993      82      54      32      32
 
MscI TGG'CCA

Cuts at:      0     826    2193 
   Size:        826    1367
 
MslI CAynn'nnrTG

Cuts at:      0     328     457    2193 
   Size:        328     129    1736

  Fragments arranged by size: 

                1736     328     129
 
MspA1I CmG'CkG

Cuts at:      0     431     756     864     972     999    1158    1262    1893 
   Size:        431     325     108     108      27     159     104     631

Cuts at:   1893    2193 
   Size:        300

  Fragments arranged by size: 

                 631     431     325     300     159     108     108     104
                  27
 
NaeI GCC'GGC

Cuts at:      0     501    2193 
   Size:        501    1692
 
NcoI C'CATG_G

Cuts at:      0     164     515     671    2193 
   Size:        164     351     156    1522

  Fragments arranged by size: 

                1522     351     164     156
 
NgoMIV G'CCGG_C

Cuts at:      0     499    2193 
   Size:        499    1694
 
NheI G'CTAG_C

Cuts at:      0    1818    2193 
   Size:       1818     375
 
NspI r_CATG'y

Cuts at:      0     591    2193 
   Size:        591    1602
 
PasI CC'CwG_GG

Cuts at:      0    1112    1348    1755    1880    2150    2193 
   Size:       1112     236     407     125     270      43

  Fragments arranged by size: 

                1112     407     270     236     125      43
 
PciI A'CATG_T

Cuts at:      0     587    2193 
   Size:        587    1606
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1020    1305    2193 
   Size:       1020     285     888

  Fragments arranged by size: 

                1020     888     285
 
PfoI T'CCnGG_A

Cuts at:      0      82    1967    2193 
   Size:         82    1885     226

  Fragments arranged by size: 

                1885     226      82
 
PpuMI rG'GwC_Cy

Cuts at:      0     357     627     693    1266    1344    2193 
   Size:        357     270      66     573      78     849

  Fragments arranged by size: 

                 849     573     357     270      78      66
 
PshAI GACnn'nnGTC

Cuts at:      0     235    2193 
   Size:        235    1958
 
PspOMI G'GGCC_C

Cuts at:      0      72     602     667    1004    1041    1608    2146    2193 
   Size:         72     530      65     337      37     567     538      47

  Fragments arranged by size: 

                 567     538     530     337      72      65      47      37
            
 
PstI C_TGCA'G

Cuts at:      0     116     317     761     857     869     953     995    1322 
   Size:        116     201     444      96      12      84      42     327

Cuts at:   1322    2193 
   Size:        871

  Fragments arranged by size: 

                 871     444     327     201     116      96      84      42
                  12
 
PvuII CAG'CTG

Cuts at:      0     756     864     999    1158    1893    2193 
   Size:        756     108     135     159     735     300

  Fragments arranged by size: 

                 756     735     300     159     135     108
 
SacII CC_GC'GG

Cuts at:      0    1263    2193 
   Size:       1263     930
 
SanDI GG'GwC_CC

Cuts at:      0    1266    2193 
   Size:       1266     927
 
SapI GCTCTTCn'nnn_

Cuts at:      0     190     875    2193 
   Size:        190     685    1318

  Fragments arranged by size: 

                1318     685     190
 
SbfI CC_TGCA'GG

Cuts at:      0     116     317    2193 
   Size:        116     201    1876

  Fragments arranged by size: 

                1876     201     116
 
SfcI C'TryA_G

Cuts at:      0     112     313     757     853     865     949     991    1318 
   Size:        112     201     444      96      12      84      42     327

Cuts at:   1318    2193 
   Size:        875

  Fragments arranged by size: 

                 875     444     327     201     112      96      84      42
                  12
 
StuI AGG'CCT

Cuts at:      0     338    1375    2193 
   Size:        338    1037     818

  Fragments arranged by size: 

                1037     818     338
 
StyI C'CwwG_G

Cuts at:      0     164     515     597     671     827    1564    1624    1898 
   Size:        164     351      82      74     156     737      60     274

Cuts at:   1898    2193 
   Size:        295

  Fragments arranged by size: 

                 737     351     295     274     164     156      82      74
                  60
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      21    2193 
   Size:         21    2172
 
TatI w'GTAC_w

Cuts at:      0    1221    2193 
   Size:       1221     972
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     115     147    1372    1404    1417    1449    2193 
   Size:        115      32    1225      32      13      32     744

  Fragments arranged by size: 

                1225     744     115      32      32      32      13
 
Tth111I GACn'n_nGTC

Cuts at:      0     781    1033    2193 
   Size:        781     252    1160

  Fragments arranged by size: 

                1160     781     252
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     522     678    1544    2193 
   Size:        522     156     866     649

  Fragments arranged by size: 

                 866     649     522     156
 
XmnI GAAnn'nnTTC

Cuts at:      0     884    2193 
   Size:        884    1309
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AleI     AloI    AlwNI     ApaI     ApoI
     AvaI     BanI    BanII     BbsI    BbvCI     BglI     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaWI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BstAPI    BstXI    BstYI
     BtgI     BtsI    CspCI   DraIII     DrdI     EaeI     EarI  Eco57MI
    EcoNI EcoO109I    EcoRI     FalI     FseI    Hin4I     MscI     MslI
   MspA1I     NaeI     NcoI   NgoMIV     NheI     NspI     PasI     PciI
    PflMI     PfoI    PpuMI    PshAI   PspOMI     PstI    PvuII    SacII
    SanDI     SapI     SbfI     SfcI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AlfI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BbeI     BcgI    BciVI     BclI    BfrBI    BglII    BpuEI    BsaAI
    BsaBI    BsaHI    BsiEI    BsiWI    BspEI    BsrDI   BssHII    BssSI
    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI     DraI     EagI
     EciI  EcoICRI    EcoRV     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     NarI     NdeI
     NotI     NruI     NsiI     PacI     PmeI     PmlI     PpiI     PsiI
    PspXI     PsrI     PvuI    RsrII     SacI     SalI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI     XbaI     XhoI     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com