Restriction map for
pF1KB9477
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BalI BamHI BbsI BbvCI BciVI
BfrI BglII BlnI BpuAI BsaBI
BsaI BsaWI BseAI BsiHKAI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Bsu36I ClaI
CpoI DraI Eam1105I Eco47III Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HincII HindII HindIII
HinfI HpaI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MunI MvnI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PleI PmeI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tsp509I Tth111I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 958 GACGTC GACGT^C
AfaI 614 GTAC GT^AC
AlwNI 550 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 722 GGACC G^G[AT]CC
BbrPI 376 CACGTG CAC^GTG
BclI 659 TGATCA T^GATCA
BfaI 40 CTAG C^TAG
BlpI 58 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 58 GCTGAGC GC^T[ACGT]AGC
BsaAI 376 CACGTG [CT]AC^GT[GA]
BseRI 52 GAGGAG GAGGAG(10/8)
BsgI 297 CTGCAC GTGCAG(16/14)
BsiEI 990 CGGCCG CG[GA][CT]^CG
BspMI 809 ACCTGC ACCTGC(4/8)
BsrFI 566 GCCGGC [GA]^CCGG[CT]
BssSI 172 CTCGTG CACGAG(-5/-1)
BstAPI 549 GCAGAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 854 CCACCATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 762 GGATCT [GA]^GATC[CT]
CelII 58 GCTGAGC GC^T[ACGT]AGC
Cfr10I 566 GCCGGC [GA]^CCGG[CT]
DdeI 59 CTGAG C^T[ACGT]AG
DraIII 313 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 990 CGGCCG C^GGCCG
EarI 781 GAAGAG CTCTTC(1/4)
EclXI 990 CGGCCG C^GGCCG
Eco52I 990 CGGCCG C^GGCCG
FbaI 659 TGATCA T^GATCA
HgaI 979 GACGC GACGC(5/10)
Ksp632I 781 GAAGAG CTCTTC(1/4)
MaeI 40 CTAG C^TAG
MflI 762 GGATCT [GA]^GATC[CT]
MseI 542 TTAA T^TAA
NaeI 566 GCCGGC GCC^GGC
NgoMIV 566 GCCGGC G^CCGGC
PmaCI 376 CACGTG CAC^GTG
PmlI 376 CACGTG CAC^GTG
PpuMI 721 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 187 CAGCTG CAG^CTG
RsaI 614 GTAC GT^AC
SmaI 740 CCCGGG CCC^GGG
SmlI 592 CTCAAG C^T[CT][GA]AG
Tru9I 542 TTAA T^TAA
Tsp45I 926 GTGAC ^GT[GC]AC
XhoII 762 GGATCT [GA]^GATC[CT]
XmaCI 740 CCCGGG C^CCGGG
XmaI 740 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwI 737 GATCC GGATC(4/5)
762 GGATC
ApaI 123 GGGCCC GGGCC^C
744 GGGCCC
BanI 30 GGCGCC G^G[CT][GA]CC
967 GGCGCC
BbeI 30 GGCGCC GGCGC^C
967 GGCGCC
BcgI 488 GCAGCGTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
644 GCAAGGCCTTCG
BglI 554 GCCTGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
683 GCCGCCCTGGC
BpmI 355 CTGGAG CTGGAG(16/14)
814 CTGGAG
DrdI 943 GACGAGTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
979 GACGCCAAGGTC
HaeII 30 GGCGCC [GA]GCGC^[CT]
967 GGCGCC
KasI 30 GGCGCC G^GCGCC
967 GGCGCC
MaeIII 821 GTTAC ^GT[ACGT]AC
926 GTGAC
MboII 781 GAAGA GAAGA(8/7)
368 TCTTC
MslI 513 CATCAGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
855 CACCATGGTG
NarI 30 GGCGCC GG^CGCC
967 GGCGCC
NcoI 195 CCATGG C^CATGG
857 CCATGG
PflMI 64 CCAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
312 CCACAAAGTGG
SfoI 30 GGCGCC GGC^GCC
967 GGCGCC
StuI 263 AGGCCT AGG^CCT
647 AGGCCT
TspRI 207 CACTG CA[GC]TG[ACGT][ACGT]^
711 CACTG
Van91I 64 CCAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
312 CCACAAAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AcyI AluI AvaI BanII
BbvI BcnI BmyI BsaHI BsaJI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
Hin1I HinP1I HpaII HphI ItaI
MaeII MboI MnlI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TseI TthHB8I XcmI