Restriction map for pF1KB9476
 (Linear) (Six-base) MAPSORT of: KIBB9476.seq  Check: 2372  from: 1  to: 1047



KIBB9476.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:39  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     438    1047 
   Size:        438     609
 
Acc65I G'GTAC_C

Cuts at:      0     398    1047 
   Size:        398     649
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58     310    1047 
   Size:         58     252     737

  Fragments arranged by size: 

                 737     252      58
 
AleI CACnn'nnGTG

Cuts at:      0     661    1047 
   Size:        661     386
 
AlwNI CAG_nnn'CTG

Cuts at:      0     522    1047 
   Size:        522     525
 
ApaI G_GGCC'C

Cuts at:      0     985    1047 
   Size:        985      62
 
ApaLI G'TGCA_C

Cuts at:      0     643     934    1047 
   Size:        643     291     113

  Fragments arranged by size: 

                 643     291     113
 
ApoI r'AATT_y

Cuts at:      0      43    1047 
   Size:         43    1004
 
AvaI C'yCGr_G

Cuts at:      0     680     689    1023    1047 
   Size:        680       9     334      24

  Fragments arranged by size: 

                 680     334      24       9
 
BanI G'GyrC_C

Cuts at:      0     118     398     426     684    1047 
   Size:        118     280      28     258     363

  Fragments arranged by size: 

                 363     280     258     118      28
 
BanII G_rGCy'C

Cuts at:      0     362     461     656     985    1047 
   Size:        362      99     195     329      62

  Fragments arranged by size: 

                 362     329     195      99      62
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     493    1047 
   Size:        493     554
 
BglI GCCn_nnn'nGGC

Cuts at:      0     313     562     989    1047 
   Size:        313     249     427      58

  Fragments arranged by size: 

                 427     313     249      58
 
Bme1580I G_kGCm'C

Cuts at:      0     567     647     689     938     985    1047 
   Size:        567      80      42     249      47      62

  Fragments arranged by size: 

                 567     249      80      62      47      42
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     445     553     801     886    1047 
   Size:        445     108     248      85     161

  Fragments arranged by size: 

                 445     248     161     108      85
 
Bpu10I CC'TnA_GC

Cuts at:      0     233    1047 
   Size:        233     814
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     562     769    1047 
   Size:        562     207     278

  Fragments arranged by size: 

                 562     278     207
 
BsaHI Gr'CG_yC

Cuts at:      0     185    1047 
   Size:        185     862
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     826    1047 
   Size:        826     221
 
BseYI C'CCAG_C

Cuts at:      0     114     143     188     394     557     668    1047 
   Size:        114      29      45     206     163     111     379

  Fragments arranged by size: 

                 379     206     163     114     111      45      29
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     810    1047 
   Size:        810     237
 
BsiHKAI G_wGCw'C

Cuts at:      0     362     461     647     938    1047 
   Size:        362      99     186     291     109

  Fragments arranged by size: 

                 362     291     186     109      99
 
BsmI GAATG_Cn'

Cuts at:      0     234     812    1047 
   Size:        234     578     235

  Fragments arranged by size: 

                 578     235     234
 
Bsp1286I G_dGCh'C

Cuts at:      0     362     461     567     647     656     689     938     985 
   Size:        362      99     106      80       9      33     249      47

Cuts at:    985    1047 
   Size:         62

  Fragments arranged by size: 

                 362     249     106      99      80      62      47      33
                   9
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     212     438    1047 
   Size:        212     226     609

  Fragments arranged by size: 

                 609     226     212
 
BsrBI CCG'CTC

Cuts at:      0     624    1047 
   Size:        624     423
 
BssSI C'ACGA_G

Cuts at:      0     109    1047 
   Size:        109     938
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     111    1047 
   Size:        111     936
 
BstBI TT'CG_AA

Cuts at:      0     242    1047 
   Size:        242     805
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     639     663    1047 
   Size:        639      24     384

  Fragments arranged by size: 

                 639     384      24
 
BstYI r'GATC_y

Cuts at:      0     467    1047 
   Size:        467     580
 
Bsu36I CC'TnA_GG

Cuts at:      0     551    1047 
   Size:        551     496
 
BtgI C'CryG_G

Cuts at:      0      26     656     845    1017    1047 
   Size:         26     630     189     172      30

  Fragments arranged by size: 

                 630     189     172      30      26
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     908    1047 
   Size:        908     139
 
BtsI GCAGTG_nn'

Cuts at:      0     175    1047 
   Size:        175     872
 
EaeI y'GGCC_r

Cuts at:      0     305     342    1047 
   Size:        305      37     705

  Fragments arranged by size: 

                 705     305      37
 
EarI CTCTTCn'nnn_

Cuts at:      0      26     256     367    1047 
   Size:         26     230     111     680

  Fragments arranged by size: 

                 680     230     111      26
 
EcoICRI GAG'CTC

Cuts at:      0     360     459    1047 
   Size:        360      99     588

  Fragments arranged by size: 

                 588     360      99
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58     310     445     553     801     886    1047 
   Size:         58     252     135     108     248      85     161

  Fragments arranged by size: 

                 252     248     161     135     108      85      58
 
EcoO109I rG'GnC_Cy

Cuts at:      0     528     981    1047 
   Size:        528     453      66

  Fragments arranged by size: 

                 528     453      66
 
EcoRI G'AATT_C

Cuts at:      0      43    1047 
   Size:         43    1004
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      63     981    1047 
   Size:         63     918      66

  Fragments arranged by size: 

                 918      66      63
 
HaeII r_GCGC'y

Cuts at:      0    1011    1047 
   Size:       1011      36
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     370     402    1047 
   Size:        370      32     645

  Fragments arranged by size: 

                 645     370      32
 
HincII GTy'rAC

Cuts at:      0     594    1047 
   Size:        594     453
 
KpnI G_GTAC'C

Cuts at:      0     402    1047 
   Size:        402     645
 
MscI TGG'CCA

Cuts at:      0     344    1047 
   Size:        344     703
 
MslI CAynn'nnrTG

Cuts at:      0     604     661     731    1047 
   Size:        604      57      70     316

  Fragments arranged by size: 

                 604     316      70      57
 
MspA1I CmG'CkG

Cuts at:      0     143     438     561     672     821    1019    1047 
   Size:        143     295     123     111     149     198      28

  Fragments arranged by size: 

                 295     198     149     143     123     111      28
 
PasI CC'CwG_GG

Cuts at:      0     985    1047 
   Size:        985      62
 
PspOMI G'GGCC_C

Cuts at:      0     981    1047 
   Size:        981      66
 
PstI C_TGCA'G

Cuts at:      0     527     548    1047 
   Size:        527      21     499

  Fragments arranged by size: 

                 527     499      21
 
PvuII CAG'CTG

Cuts at:      0     672     821    1047 
   Size:        672     149     226

  Fragments arranged by size: 

                 672     226     149
 
SacI G_AGCT'C

Cuts at:      0     362     461    1047 
   Size:        362      99     586

  Fragments arranged by size: 

                 586     362      99
 
SacII CC_GC'GG

Cuts at:      0    1020    1047 
   Size:       1020      27
 
SapI GCTCTTCn'nnn_

Cuts at:      0      26     256     367    1047 
   Size:         26     230     111     680

  Fragments arranged by size: 

                 680     230     111      26
 
SbfI CC_TGCA'GG

Cuts at:      0     527    1047 
   Size:        527     520
 
SfcI C'TryA_G

Cuts at:      0     523     544     755    1047 
   Size:        523      21     211     292

  Fragments arranged by size: 

                 523     292     211      21
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     313    1047 
   Size:        313     734
 
SmaI CCC'GGG

Cuts at:      0     682    1025    1047 
   Size:        682     343      22

  Fragments arranged by size: 

                 682     343      22
 
SmlI C'TyrA_G

Cuts at:      0     577     748    1047 
   Size:        577     171     299

  Fragments arranged by size: 

                 577     299     171
 
StuI AGG'CCT

Cuts at:      0     964    1047 
   Size:        964      83
 
StyI C'CwwG_G

Cuts at:      0      53     977    1047 
   Size:         53     924      70

  Fragments arranged by size: 

                 924      70      53
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     106     745    1047 
   Size:        106     639     302

  Fragments arranged by size: 

                 639     302     106
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     400     432    1047 
   Size:        400      32     615

  Fragments arranged by size: 

                 615     400      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     140    1047 
   Size:        140     907
 
XmaI C'CCGG_G

Cuts at:      0     680    1023    1047 
   Size:        680     343      24

  Fragments arranged by size: 

                 680     343      24
 
XmnI GAAnn'nnTTC

Cuts at:      0      43    1047 
   Size:         43    1004
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI     AleI    AlwNI     ApaI    ApaLI     ApoI
     AvaI     BanI    BanII     BbsI     BglI Bme1580I     BpmI   Bpu10I
    BpuEI    BsaHI    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I
    BspMI    BsrBI    BssSI   BstAPI    BstBI    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     EaeI     EarI  EcoICRI  Eco57MI EcoO109I
    EcoRI     FalI    HaeII    Hin4I   HincII     KpnI     MscI     MslI
   MspA1I     PasI   PspOMI     PstI    PvuII     SacI    SacII     SapI
     SbfI     SfcI     SfiI     SmaI     SmlI     StuI     StyI    TaqII
     TstI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AlfI     AloI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI
    BmgBI     BmrI     BmtI     BplI     BsaI    BsaAI    BsaBI    BsaWI
    BsaXI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BsrDI    BsrFI
    BsrGI   BssHII   BstEII  BstZ17I     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EciI    EcoNI    EcoRV     FseI     FspI    FspAI
  HindIII     HpaI     KasI     MfeI     MluI     MmeI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SalI    SanDI
     ScaI    SexAI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI     TatI  Tth111I     XbaI     XhoI     ZraI
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