Restriction map for pF1KB9471
##################################
# NO CUT ENZYMES (   94 enzymes) #
##################################

AatII       AclI        AflII       AflIII      AgeI        
AhdI        Alw44I      ApaLI       AscI        AseI        
AsnI        AspEI       AspI        AvrII       BamHI       
BbrPI       BclI        BfrI        BglII       BlnI        
BsaAI       BsaBI       BsaI        BsiWI       BsmBI       
Bsp1407I    BspDI       BspHI       BspLU11I    BsrGI       
BssHII      Bst1107I    BstBI       BstEII      BstPI       
BstZ17I     ClaI        CpoI        DraI        Eam1105I    
EcoO65I     EcoRV       EcoT22I     FbaI        FseI        
HgaI        HincII      HindII      HindIII     HpaI        
KspI        MamI        MfeI        MluI        MunI        
NaeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SfiI        SfuI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

Acc65I       1566  GGTACC               G^GTACC
AccI         1867  GTCTAC               GT^[AC][GT]AC
AccII        1361  CGCG                 CG^CG
AcsI          594  GAATTC               [GA]^AATT[CT]
AcyI         1370  GGCGCC               G[GA]^CG[CT]C
ApoI          594  GAATTC               [GA]^AATT[CT]
Asp718I      1566  GGTACC               G^GTACC
BalI         2994  TGGCCA               TGG^CCA
BbeI         1370  GGCGCC               GGCGC^C
BcgI         1847  GCACTTTCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI         1675  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1675  GCTGAGC              GC^T[ACGT]AGC
BsaHI        1370  GGCGCC               G[GA]^CG[CT]C
BsiEI        2640  CGGCCG               CG[GA][CT]^CG
BstUI        1361  CGCG                 CG^CG
CelII        1675  GCTGAGC              GC^T[ACGT]AGC
DraIII        667  CACCAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI         2640  CGGCCG               C^GGCCG
EclXI        2640  CGGCCG               C^GGCCG
Eco52I       2640  CGGCCG               C^GGCCG
EcoRI         594  GAATTC               G^AATTC
Hin1I        1370  GGCGCC               G[GA]^CG[CT]C
KasI         1370  GGCGCC               G^GCGCC
KpnI         1566  GGTACC               GGTAC^C
MaeII        2171  ACGT                 A^CGT
MluNI        2994  TGGCCA               TGG^CCA
MscI         2994  TGGCCA               TGG^CCA
MseI         2720  TTAA                 T^TAA
MvnI         1361  CGCG                 CG^CG
NarI         1370  GGCGCC               GG^CGCC
NdeI         3166  CATATG               CA^TATG
SexAI         668  ACCAGGT              A^CC[AT]GGT
SfoI         1370  GGCGCC               GGC^GCC
SmaI         1726  CCCGGG               CCC^GGG
SmlI         1739  CTCAAG               C^T[CT][GA]AG
StuI         2999  AGGCCT               AGG^CCT
TaqI         2156  TCGA                 T^CGA
Tru9I        2720  TTAA                 T^TAA
TthHB8I      2156  TCGA                 T^CGA
XmaCI        1726  CCCGGG               C^CCGGG
XmaI         1726  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccIII       2087  TCCGGA               T^CCGGA
             2957  TCCGGA              
AfaI          135  GTAC                 GT^AC
             1567  GTAC                
AocI          484  CCTGAGG              CC^T[ACGT]AGG
             1006  CCTCAGG             
Aor51HI       999  AGCGCT               AGC^GCT
             3204  AGCGCT              
AviII         962  TGCGCA               TGC^GCA
             3162  TGCGCA              
BciVI        2673  GGATAC               GTATCC(6/5)
             1058  GTATCC              
BsaWI        2087  TCCGGA               [AT]^CCGG[AT]
             2957  TCCGGA              
BseAI        2087  TCCGGA               T^CCGGA
             2957  TCCGGA              
BsmI         1440  GAATGC               GAATGC(1/-1)
              658  GCATTC              
BspEI        2087  TCCGGA               T^CCGGA
             2957  TCCGGA              
BsrDI        2532  CATTGC               GCAATG(2/0)
             2645  GCAATG              
BsrFI         677  ACCGGC               [GA]^CCGG[CT]
             2989  GCCGGT              
Bsu36I        484  CCTGAGG              CC^T[ACGT]AGG
             1006  CCTCAGG             
Cfr10I        677  ACCGGC               [GA]^CCGG[CT]
             2989  GCCGGT              
DrdI         1756  GACATGAATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3024  GACAGGGAAGTC        
Eco47III      999  AGCGCT               AGC^GCT
             3204  AGCGCT              
Eco81I        484  CCTGAGG              CC^T[ACGT]AGG
             1006  CCTCAGG             
FspI          962  TGCGCA               TGC^GCA
             3162  TGCGCA              
MroI         2087  TCCGGA               T^CCGGA
             2957  TCCGGA              
NcoI         1280  CCATGG               C^CATGG
             2263  CCATGG              
PpuMI        1656  GGGTCCT              [GA]G^G[AT]CC[CT]
             2270  AGGACCT             
RsaI          135  GTAC                 GT^AC
             1567  GTAC                
SapI         3150  GAAGAGC              GCTCTTC(1/4)
             3171  GAAGAGC             
Tsp509I       595  AATT                 ^AATT
             1393  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   99 enzymes) #
#########################################

AciI        AluI        AlwI        AlwNI       ApaI        
Asp700I     AspHI       AvaI        AvaII       BanI        
BanII       BbsI        BbvCI       BbvI        BcnI        
BfaI        BglI        BmyI        BpmI        BpuAI       
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrBI       BsrI        BssKI       BssSI       
BstAPI      BstF5I      BstNI       BstXI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeIII      MboI        MboII       
MflI        MnlI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PflMI       PleI        PstI        PvuII       
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
StyI        TfiI        TseI        Tsp45I      TspRI       
Van91I      XcmI        XhoII       XmnI        
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