Restriction map for pF1KB9471
 (Linear) (Six-base) MAPSORT of: KIBB9471.seq  Check: 6587  from: 1  to: 3297



KIBB9471.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:39  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     261    1320    1907    2619    3297 
   Size:        261    1059     587     712     678

  Fragments arranged by size: 

                1059     712     678     587     261
 
AccI GT'mk_AC

Cuts at:      0    1868    3297 
   Size:       1868    1429
 
Acc65I G'GTAC_C

Cuts at:      0    1566    3297 
   Size:       1566    1731
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     514    1205    1484    2123    2452    3235    3297 
   Size:        514     691     279     639     329     783      62

  Fragments arranged by size: 

                 783     691     639     514     329     279      62
 
AfeI AGC'GCT

Cuts at:      0    1001    3206    3297 
   Size:       1001    2205      91

  Fragments arranged by size: 

                2205    1001      91
 
AleI CACnn'nnGTG

Cuts at:      0    2146    3297 
   Size:       2146    1151
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     808     840    3297 
   Size:        808      32    2457

  Fragments arranged by size: 

                2457     808      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     359     633    1227    1588    2367    2488    2571    3264 
   Size:        359     274     594     361     779     121      83     693

Cuts at:   3264    3297 
   Size:         33

  Fragments arranged by size: 

                 779     693     594     361     359     274     121      83
                  33
 
ApaI G_GGCC'C

Cuts at:      0     322     517     700    3297 
   Size:        322     195     183    2597

  Fragments arranged by size: 

                2597     322     195     183
 
ApoI r'AATT_y

Cuts at:      0     594    3297 
   Size:        594    2703
 
AvaI C'yCGr_G

Cuts at:      0     139    1726    1816    3297 
   Size:        139    1587      90    1481

  Fragments arranged by size: 

                1587    1481     139      90
 
BanI G'GyrC_C

Cuts at:      0     585     918    1090    1370    1566    2353    2745    2835 
   Size:        585     333     172     280     196     787     392      90

Cuts at:   2835    3297 
   Size:        462

  Fragments arranged by size: 

                 787     585     462     392     333     280     196     172
                  90
 
BanII G_rGCy'C

Cuts at:      0     322     445     517     700    1823    2179    2792    2813 
   Size:        322     123      72     183    1123     356     613      21

Cuts at:   2813    3297 
   Size:        484

  Fragments arranged by size: 

                1123     613     484     356     322     183     123      72
                  21
 
BbeI G_GCGC'C

Cuts at:      0    1374    3297 
   Size:       1374    1923
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     678    1559    2315    3297 
   Size:        678     881     756     982

  Fragments arranged by size: 

                 982     881     756     678
 
BbvCI CC'TCA_GC

Cuts at:      0     249    1210    1978    3208    3297 
   Size:        249     961     768    1230      89

  Fragments arranged by size: 

                1230     961     768     249      89
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1836    1870    3297 
   Size:       1836      34    1427

  Fragments arranged by size: 

                1836    1427      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1069    2667    3297 
   Size:       1069    1598     630

  Fragments arranged by size: 

                1598    1069     630
 
BglI GCCn_nnn'nGGC

Cuts at:      0     879    1414    2445    3297 
   Size:        879     535    1031     852

  Fragments arranged by size: 

                1031     879     852     535
 
BlpI GC'TnA_GC

Cuts at:      0    1676    3297 
   Size:       1676    1621
 
Bme1580I G_kGCm'C

Cuts at:      0     146     322     517     700    1849    2073    2220    3045 
   Size:        146     176     195     183    1149     224     147     825

Cuts at:   3045    3297 
   Size:        252

  Fragments arranged by size: 

                1149     825     252     224     195     183     176     147
                 146
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     895    1576    2140    2589    3297 
   Size:        895     681     564     449     708

  Fragments arranged by size: 

                 895     708     681     564     449
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     264    1446    3297 
   Size:        264    1182    1851

  Fragments arranged by size: 

                1851    1182     264
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     293     473     586    1225    1652    2316    2806    3297 
   Size:        293     180     113     639     427     664     490     491

  Fragments arranged by size: 

                 664     639     491     490     427     293     180     113
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     239     249    1210    1530    1745    1978    3208    3297 
   Size:        239      10     961     320     215     233    1230      89

  Fragments arranged by size: 

                1230     961     320     239     233     215      89      10
            
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1724    3297 
   Size:       1724    1573
 
BsaHI Gr'CG_yC

Cuts at:      0    1371    3297 
   Size:       1371    1926
 
BsaWI w'CCGG_w

Cuts at:      0    2087    2957    3297 
   Size:       2087     870     340

  Fragments arranged by size: 

                2087     870     340
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     118     148     149     179     446     476    2161    2191 
   Size:        118      30       1      30     267      30    1685      30

Cuts at:   2191    3297 
   Size:       1106

  Fragments arranged by size: 

                1685    1106     267     118      30      30      30      30
                   1
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     142     347    1306    2039    2411    2842    2845    2872 
   Size:        142     205     959     733     372     431       3      27

Cuts at:   2872    3286    3297 
   Size:        414      11

  Fragments arranged by size: 

                 959     733     431     414     372     205     142      27
                  11       3
 
BseYI C'CCAG_C

Cuts at:      0      14     104     266     877    1069    1094    3297 
   Size:         14      90     162     611     192      25    2203

  Fragments arranged by size: 

                2203     611     192     162      90      25      14
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     580    2359    2368    2584    3297 
   Size:        580    1779       9     216     713

  Fragments arranged by size: 

                1779     713     580     216       9
 
BsiEI CG_ry'CG

Cuts at:      0    2643    3297 
   Size:       2643     654
 
BsiHKAI G_wGCw'C

Cuts at:      0    1315    1360    1548    1682    3297 
   Size:       1315      45     188     134    1615

  Fragments arranged by size: 

                1615    1315     188     134      45
 
BsmI GAATG_Cn'

Cuts at:      0     658    1446    3297 
   Size:        658     788    1851

  Fragments arranged by size: 

                1851     788     658
 
Bsp1286I G_dGCh'C

Cuts at:      0     146     322     445     517     700    1315    1360    1548 
   Size:        146     176     123      72     183     615      45     188

Cuts at:   1548    1682    1823    1849    2073    2179    2220    2792    2813 
   Size:        134     141      26     224     106      41     572      21

Cuts at:   2813    3045    3297 
   Size:        232     252

  Fragments arranged by size: 

                 615     572     252     232     224     188     183     176
                 146     141     134     123     106      72      45      41
                  26      21
 
BspEI T'CCGG_A

Cuts at:      0    2087    2957    3297 
   Size:       2087     870     340

  Fragments arranged by size: 

                2087     870     340
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     261     672    1320    1907    2321    2619    3297 
   Size:        261     411     648     587     414     298     678

  Fragments arranged by size: 

                 678     648     587     414     411     298     261
 
BsrBI CCG'CTC

Cuts at:      0     243     807    1034    1938    3278    3297 
   Size:        243     564     227     904    1340      19

  Fragments arranged by size: 

                1340     904     564     243     227      19
 
BsrDI GCAATG_nn'

Cuts at:      0    2531    2652    3297 
   Size:       2531     121     645

  Fragments arranged by size: 

                2531     645     121
 
BsrFI r'CCGG_y

Cuts at:      0     677    2989    3297 
   Size:        677    2312     308

  Fragments arranged by size: 

                2312     677     308
 
BssSI C'ACGA_G

Cuts at:      0     145    1547    2965    3297 
   Size:        145    1402    1418     332

  Fragments arranged by size: 

                1418    1402     332     145
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1227    2382    3264    3297 
   Size:       1227    1155     882      33

  Fragments arranged by size: 

                1227    1155     882      33
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     862    1077    2589    3297 
   Size:        862     215    1512     708

  Fragments arranged by size: 

                1512     862     708     215
 
BstYI r'GATC_y

Cuts at:      0    1016    2232    2843    3297 
   Size:       1016    1216     611     454

  Fragments arranged by size: 

                1216    1016     611     454
 
Bsu36I CC'TnA_GG

Cuts at:      0     485    1007    3297 
   Size:        485     522    2290

  Fragments arranged by size: 

                2290     522     485
 
BtgI C'CryG_G

Cuts at:      0    1280    2161    2263    2588    3297 
   Size:       1280     881     102     325     709

  Fragments arranged by size: 

                1280     881     709     325     102
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1839    2059    2696    3297 
   Size:       1839     220     637     601

  Fragments arranged by size: 

                1839     637     601     220
 
BtsI GCAGTG_nn'

Cuts at:      0     631    1313    1432    1461    3165    3297 
   Size:        631     682     119      29    1704     132

  Fragments arranged by size: 

                1704     682     631     132     119      29
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     698     733    3297 
   Size:        698      35    2564

  Fragments arranged by size: 

                2564     698      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     672    3297 
   Size:        672    2625
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1762    3030    3297 
   Size:       1762    1268     267

  Fragments arranged by size: 

                1762    1268     267
 
EaeI y'GGCC_r

Cuts at:      0     663    1257    2504    2640    2936    2994    3297 
   Size:        663     594    1247     136     296      58     303

  Fragments arranged by size: 

                1247     663     594     303     296     136      58
 
EagI C'GGCC_G

Cuts at:      0    2640    3297 
   Size:       2640     657
 
EarI CTCTTCn'nnn_

Cuts at:      0      55    1460    2696    2754    2756    2771    2861    3145 
   Size:         55    1405    1236      58       2      15      90     284

Cuts at:   3145    3166    3297 
   Size:         21     131

  Fragments arranged by size: 

                1405    1236     284     131      90      58      55      21
                  15       2
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1358    3297 
   Size:       1358    1939
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     293     473     514     586    1205    1225    1484    1652 
   Size:        293     180      41      72     619      20     259     168

Cuts at:   1652    2123    2316    2452    2806    3235    3297 
   Size:        471     193     136     354     429      62

  Fragments arranged by size: 

                 619     471     429     354     293     259     193     180
                 168     136      72      62      41      20
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     976    1048    2205    3268    3297 
   Size:        976      72    1157    1063      29

  Fragments arranged by size: 

                1157    1063     976      72      29
 
EcoO109I rG'GnC_Cy

Cuts at:      0     318     513     696     969    1657    2271    3261    3297 
   Size:        318     195     183     273     688     614     990      36

  Fragments arranged by size: 

                 990     688     614     318     273     195     183      36
            
 
EcoRI G'AATT_C

Cuts at:      0     594    3297 
   Size:        594    2703
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2265    2571    3297 
   Size:       2265     306     726

  Fragments arranged by size: 

                2265     726     306
 
FspI TGC'GCA

Cuts at:      0     964    3164    3297 
   Size:        964    2200     133

  Fragments arranged by size: 

                2200     964     133
 
FspAI rTGC'GCAy

Cuts at:      0    3164    3297 
   Size:       3164     133
 
HaeII r_GCGC'y

Cuts at:      0    1003    1076    1374    3208    3297 
   Size:       1003      73     298    1834      89

  Fragments arranged by size: 

                1834    1003     298      89      73
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     149     181     432     446     464     478    1722    1754 
   Size:        149      32     251      14      18      14    1244      32

Cuts at:   1754    1915    1947    2058    2090    2387    2419    2674    2706 
   Size:        161      32     111      32     297      32     255      32

Cuts at:   2706    3081    3113    3297 
   Size:        375      32     184

  Fragments arranged by size: 

                1244     375     297     255     251     184     161     149
                 111      32      32      32      32      32      32      32
                  18      14      14
 
KasI G'GCGC_C

Cuts at:      0    1370    3297 
   Size:       1370    1927
 
KpnI G_GTAC'C

Cuts at:      0    1570    3297 
   Size:       1570    1727
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1751    1898    2996    3297 
   Size:       1751     147    1098     301

  Fragments arranged by size: 

                1751    1098     301     147
 
MscI TGG'CCA

Cuts at:      0    2996    3297 
   Size:       2996     301
 
MslI CAynn'nnrTG

Cuts at:      0     118    1582    2146    3297 
   Size:        118    1464     564    1151

  Fragments arranged by size: 

                1464    1151     564     118
 
MspA1I CmG'CkG

Cuts at:      0     359     522     564     654     877    1203    1806    2227 
   Size:        359     163      42      90     223     326     603     421

Cuts at:   2227    2463    2896    3232    3297 
   Size:        236     433     336      65

  Fragments arranged by size: 

                 603     433     421     359     336     326     236     223
                 163      90      65      42
 
NarI GG'CG_CC

Cuts at:      0    1371    3297 
   Size:       1371    1926
 
NcoI C'CATG_G

Cuts at:      0    1280    2263    3297 
   Size:       1280     983    1034

  Fragments arranged by size: 

                1280    1034     983
 
NdeI CA'TA_TG

Cuts at:      0    3167    3297 
   Size:       3167     130
 
PasI CC'CwG_GG

Cuts at:      0    1167    2980    3297 
   Size:       1167    1813     317

  Fragments arranged by size: 

                1813    1167     317
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     273     915    2367    2463    2601    3297 
   Size:        273     642    1452      96     138     696

  Fragments arranged by size: 

                1452     696     642     273     138      96
 
PfoI T'CCnGG_A

Cuts at:      0     153     450    2405    3297 
   Size:        153     297    1955     892

  Fragments arranged by size: 

                1955     892     297     153
 
PpuMI rG'GwC_Cy

Cuts at:      0    1657    2271    3297 
   Size:       1657     614    1026

  Fragments arranged by size: 

                1657    1026     614
 
PspOMI G'GGCC_C

Cuts at:      0     318     513     696    3297 
   Size:        318     195     183    2601

  Fragments arranged by size: 

                2601     318     195     183
 
PstI C_TGCA'G

Cuts at:      0      98     872     980    1148    1439    2232    3204    3297 
   Size:         98     774     108     168     291     793     972      93

  Fragments arranged by size: 

                 972     793     774     291     168     108      98      93
            
 
PvuII CAG'CTG

Cuts at:      0     359     522     564     654     877    2227    3297 
   Size:        359     163      42      90     223    1350    1070

  Fragments arranged by size: 

                1350    1070     359     223     163      90      42
 
SapI GCTCTTCn'nnn_

Cuts at:      0    3145    3166    3297 
   Size:       3145      21     131

  Fragments arranged by size: 

                3145     131      21
 
SexAI A'CCwGG_T

Cuts at:      0     668    3297 
   Size:        668    2629
 
SfcI C'TryA_G

Cuts at:      0      94     423     868     976    1144    1435    2228    2920 
   Size:         94     329     445     108     168     291     793     692

Cuts at:   2920    3200    3297 
   Size:        280      97

  Fragments arranged by size: 

                 793     692     445     329     291     280     168     108
                  97      94
 
SfoI GGC'GCC

Cuts at:      0    1372    3297 
   Size:       1372    1925
 
SmaI CCC'GGG

Cuts at:      0    1728    3297 
   Size:       1728    1569
 
SmlI C'TyrA_G

Cuts at:      0    1739    3297 
   Size:       1739    1558
 
StuI AGG'CCT

Cuts at:      0    3001    3297 
   Size:       3001     296
 
StyI C'CwwG_G

Cuts at:      0      20      74     434    1280    1838    2263    2740    3013 
   Size:         20      54     360     846     558     425     477     273

Cuts at:   3013    3297 
   Size:        284

  Fragments arranged by size: 

                 846     558     477     425     360     284     273      54
                  20
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1108    1266    3297 
   Size:       1108     158    2031

  Fragments arranged by size: 

                2031    1108     158
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     400     432    2082    2114    3297 
   Size:        400      32    1650      32    1183

  Fragments arranged by size: 

                1650    1183     400      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     300    1506    2364    3297 
   Size:        300    1206     858     933

  Fragments arranged by size: 

                1206     933     858     300
 
XmaI C'CCGG_G

Cuts at:      0    1726    3297 
   Size:       1726    1571
 
XmnI GAAnn'nnTTC

Cuts at:      0     598    2518    2551    3109    3297 
   Size:        598    1920      33     558     188

  Fragments arranged by size: 

                1920     598     558     188      33
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AfeI     AleI     AloI    AlwNI
     ApaI     ApoI     AvaI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BglI     BlpI Bme1580I     BmrI     BplI     BpmI
   Bpu10I    BpuEI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstAPI    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI    CspCI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI    FspAI    HaeII
    Hin4I     KasI     KpnI     MmeI     MscI     MslI   MspA1I     NarI
     NcoI     NdeI     PasI    PflMI     PfoI    PpuMI   PspOMI     PstI
    PvuII     SapI    SexAI     SfcI     SfoI     SmaI     SmlI     StuI
     StyI    TaqII     TstI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AclI    AflII   AflIII     AgeI     AhdI     AlfI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BclI    BfrBI
    BglII    BmgBI     BmtI     BsaI    BsaAI    BsaBI    BsiWI    BsmBI
    BspHI    BsrGI   BssHII    BstBI   BstEII  BstZ17I     ClaI     DraI
  EcoICRI    EcoRV     FseI   HincII  HindIII     HpaI     MfeI     MluI
     NaeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PciI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI
     SfiI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI     TatI  Tth111I     XbaI     XhoI     ZraI
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