Restriction map for pF1KB9449
##################################
# NO CUT ENZYMES (  110 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AhdI        Alw44I      AocI        Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AvrII       BbrPI       BbsI        
BbvCI       BciVI       BclI        BfaI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaI        BsiWI       BsmAI       BsmBI       Bsp1407I    
BspHI       BspLU11I    BsrDI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstZ17I     
Bsu36I      CelII       CpoI        DraI        DraIII      
DrdI        Eam1105I    EarI        Eco47III    Eco81I      
EcoRI       EcoRV       EcoT14I     EcoT22I     FbaI        
HincII      HindII      HindIII     HpaI        KpnI        
Ksp632I     MaeI        MboII       MfeI        MluI        
MslI        MunI        NcoI        NdeI        NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SalI        SapI        
SfiI        SfuI        SgrAI       SnaBI       SpeI        
SphI        SspBI       SspI        StuI        StyI        
SwaI        Tsp509I     XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AflII         702  CTTAAG               C^TTAAG
AflIII        950  ACGTGT               A^C[GA][CT]GT
AgeI         1357  ACCGGT               A^CCGGT
AlwNI         980  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          793  GGGCCC               GGGCC^C
AspI          463  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         311  TGCGCA               TGC^GCA
BalI          302  TGGCCA               TGG^CCA
BamHI         491  GGATCC               G^GATCC
BcgI         1017  CGATGTGGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI          702  CTTAAG               C^TTAAG
BsaBI         601  GATCTTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI         1326  GCATTC               GAATGC(1/-1)
BspDI         916  ATCGAT               AT^CGAT
BspMI         170  ACCTGC               ACCTGC(4/8)
BstEII        849  GGTGACC              G^GT[ACGT]ACC
BstPI         849  GGTGACC              G^GT[ACGT]ACC
BstXI        1013  CCAACGATGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI          916  ATCGAT               AT^CGAT
DraII         792  AGGGCCC              [GA]G^G[ACGT]CC[CT]
EcoO109I      792  AGGGCCC              [GA]G^G[ACGT]CC[CT]
EcoO65I       849  GGTGACC              G^GT[ACGT]ACC
FseI           41  GGCCGGCC             GGCCGG^CC
FspI          311  TGCGCA               TGC^GCA
HgaI          560  GCGTC                GACGC(5/10)
KspI           37  CCGCGG               CCGC^GG
MamI          601  GATCTTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         302  TGGCCA               TGG^CCA
MscI          302  TGGCCA               TGG^CCA
NaeI           42  GCCGGC               GCC^GGC
NgoMIV         42  GCCGGC               G^CCGGC
PflFI         463  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        1357  ACCGGT               A^CCGGT
PvuII         983  CAGCTG               CAG^CTG
SacII          37  CCGCGG               CCGC^GG
SbfI          171  CCTGCAGG             CCTGCA^GG
SmlI          702  CTTAAG               C^T[CT][GA]AG
Sse8387I      171  CCTGCAGG             CCTGCA^GG
TfiI          634  GAATC                G^A[AT]TC
Tth111I       463  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI          797  CCAACTTCTCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AccIII        630  TCCGGA               T^CCGGA
              637  TCCGGA              
AcyI           65  GGCGCC               G[GA]^CG[CT]C
             1341  GGCGCC              
BanI           65  GGCGCC               G^G[CT][GA]CC
             1341  GGCGCC              
BbeI           65  GGCGCC               GGCGC^C
             1341  GGCGCC              
BglI           17  GCCTGCCGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              690  GCCCAACGGGC         
BpmI          817  CTGGAG               CTGGAG(16/14)
             1139  CTGGAG              
BsaHI          65  GGCGCC               G[GA]^CG[CT]C
             1341  GGCGCC              
BseAI         630  TCCGGA               T^CCGGA
              637  TCCGGA              
BsgI         1180  CTGCAC               GTGCAG(16/14)
             1282  GTGCAG              
BsiEI          40  CGGCCG               CG[GA][CT]^CG
               44  CGGCCG              
BspEI         630  TCCGGA               T^CCGGA
              637  TCCGGA              
BsrBI         232  GAGCGG               CCGCTC(-3/-3)
              517  GAGCGG              
BsrFI          42  GCCGGC               [GA]^CCGG[CT]
             1357  ACCGGT              
BsrI          597  ACTGG                ACTGG(1/-1)
              996  CCAGT               
Cfr10I         42  GCCGGC               [GA]^CCGG[CT]
             1357  ACCGGT              
DdeI          536  CTCAG                C^T[ACGT]AG
             1085  CTCAG               
DsaI           37  CCGCGG               C^C[GA][CT]GG
             1254  CCACGG              
EagI           40  CGGCCG               C^GGCCG
               44  CGGCCG              
EclXI          40  CGGCCG               C^GGCCG
               44  CGGCCG              
Eco52I         40  CGGCCG               C^GGCCG
               44  CGGCCG              
EcoNI          93  CCTCACCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              432  CCTGCGCCAGG         
Hin1I          65  GGCGCC               G[GA]^CG[CT]C
             1341  GGCGCC              
KasI           65  GGCGCC               G^GCGCC
             1341  GGCGCC              
MroI          630  TCCGGA               T^CCGGA
              637  TCCGGA              
MseI          485  TTAA                 T^TAA
              703  TTAA                
NarI           65  GGCGCC               GG^CGCC
             1341  GGCGCC              
PflMI         979  CCAGCAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1001  CCAGCGGGTGG         
PstI          172  CTGCAG               CTGCA^G
             1068  CTGCAG              
ScaI          191  AGTACT               AGT^ACT
              505  AGTACT              
SfcI          172  CTGCAG               C^T[GA][CT]AG
             1068  CTGCAG              
SfoI           65  GGCGCC               GGC^GCC
             1341  GGCGCC              
Tru9I         485  TTAA                 T^TAA
              703  TTAA                
Van91I        979  CCAGCAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1001  CCAGCGGGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   74 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AspHI       AvaI        AvaII       BanII       BbvI        
BcnI        BmyI        BsaJI       BsaWI       BseRI       
BsiHKAI     BsiYI       BslI        BsmFI       BsoBI       
Bsp1286I    BssKI       BstF5I      BstNI       BstUI       
BstYI       Cac8I       CfoI        Cfr13I      DpnII       
EaeI        EcoRII      FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        MaeII       
MaeIII      MboI        MflI        MnlI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        RsaI        
Sau3AI      Sau96I      ScrFI       SexAI       SfaNI       
SmaI        TaqI        TseI        Tsp45I      TspRI       
TthHB8I     XhoII       XmaCI       XmaI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com