Restriction map for pF1KB9449
 (Linear) (Six-base) MAPSORT of: KIBB9449.seq  Check: 9787  from: 1  to: 1395



KIBB9449.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:38  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1291    1395 
   Size:       1291     104
 
AflII C'TTAA_G

Cuts at:      0     702    1395 
   Size:        702     693
 
AflIII A'CryG_T

Cuts at:      0     950    1395 
   Size:        950     445
 
AgeI A'CCGG_T

Cuts at:      0    1357    1395 
   Size:       1357      38
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     686    1395 
   Size:        686     709
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     957     989    1395 
   Size:        957      32     406

  Fragments arranged by size: 

                 957     406      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     985    1395 
   Size:        985     410
 
ApaI G_GGCC'C

Cuts at:      0     797    1395 
   Size:        797     598
 
AvaI C'yCGr_G

Cuts at:      0      61     268     328     961    1160    1305    1378    1395 
   Size:         61     207      60     633     199     145      73      17

  Fragments arranged by size: 

                 633     207     199     145      73      61      60      17
            
 
BamHI G'GATC_C

Cuts at:      0     491    1395 
   Size:        491     904
 
BanI G'GyrC_C

Cuts at:      0      65    1341    1395 
   Size:         65    1276      54

  Fragments arranged by size: 

                1276      65      54
 
BanII G_rGCy'C

Cuts at:      0     797     940    1159    1219    1395 
   Size:        797     143     219      60     176

  Fragments arranged by size: 

                 797     219     176     143      60
 
BbeI G_GCGC'C

Cuts at:      0      69    1345    1395 
   Size:         69    1276      50

  Fragments arranged by size: 

                1276      69      50
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1006    1040    1395 
   Size:       1006      34     355

  Fragments arranged by size: 

                1006     355      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0      23     696    1395 
   Size:         23     673     699

  Fragments arranged by size: 

                 699     673      23
 
Bme1580I G_kGCm'C

Cuts at:      0     701     797    1077    1395 
   Size:        701      96     280     318

  Fragments arranged by size: 

                 701     318     280      96
 
BmgBI CAC'GTC

Cuts at:      0     141     951    1196    1395 
   Size:        141     810     245     199

  Fragments arranged by size: 

                 810     245     199     141
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     838    1160    1395 
   Size:        838     322     235

  Fragments arranged by size: 

                 838     322     235
 
BsaBI GATnn'nnATC

Cuts at:      0     605    1395 
   Size:        605     790
 
BsaHI Gr'CG_yC

Cuts at:      0      66    1342    1395 
   Size:         66    1276      53

  Fragments arranged by size: 

                1276      66      53
 
BsaWI w'CCGG_w

Cuts at:      0     630     637    1318    1357    1395 
   Size:        630       7     681      39      38

  Fragments arranged by size: 

                 681     630      39      38       7
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     864     894    1174    1204    1395 
   Size:        864      30     280      30     191

  Fragments arranged by size: 

                 864     280     191      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     244    1071    1337    1395 
   Size:        244     827     266      58

  Fragments arranged by size: 

                 827     266     244      58
 
BseYI C'CCAG_C

Cuts at:      0      69     255     826     978    1395 
   Size:         69     186     571     152     417

  Fragments arranged by size: 

                 571     417     186     152      69
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1165    1303    1395 
   Size:       1165     138      92

  Fragments arranged by size: 

                1165     138      92
 
BsiEI CG_ry'CG

Cuts at:      0      43      47    1395 
   Size:         43       4    1348

  Fragments arranged by size: 

                1348      43       4
 
BsiHKAI G_wGCw'C

Cuts at:      0     389     544    1297    1395 
   Size:        389     155     753      98

  Fragments arranged by size: 

                 753     389     155      98
 
BsmI GAATG_Cn'

Cuts at:      0    1326    1395 
   Size:       1326      69
 
Bsp1286I G_dGCh'C

Cuts at:      0     389     544     701     797     940    1077    1159    1219 
   Size:        389     155     157      96     143     137      82      60

Cuts at:   1219    1297    1395 
   Size:         78      98

  Fragments arranged by size: 

                 389     157     155     143     137      98      96      82
                  78      60
 
BspEI T'CCGG_A

Cuts at:      0     630     637    1395 
   Size:        630       7     758

  Fragments arranged by size: 

                 758     630       7
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     179    1395 
   Size:        179    1216
 
BsrBI CCG'CTC

Cuts at:      0     234     519    1395 
   Size:        234     285     876

  Fragments arranged by size: 

                 876     285     234
 
BsrFI r'CCGG_y

Cuts at:      0      42    1357    1395 
   Size:         42    1315      38

  Fragments arranged by size: 

                1315      42      38
 
BstEII G'GTnAC_C

Cuts at:      0     849    1395 
   Size:        849     546
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1020    1395 
   Size:       1020     375
 
BstYI r'GATC_y

Cuts at:      0     413     491     600    1395 
   Size:        413      78     109     795

  Fragments arranged by size: 

                 795     413     109      78
 
BtgI C'CryG_G

Cuts at:      0      37    1254    1395 
   Size:         37    1217     141

  Fragments arranged by size: 

                1217     141      37
 
BtsI GCAGTG_nn'

Cuts at:      0     127     685    1077    1395 
   Size:        127     558     392     318

  Fragments arranged by size: 

                 558     392     318     127
 
ClaI AT'CG_AT

Cuts at:      0     917    1395 
   Size:        917     478
 
EaeI y'GGCC_r

Cuts at:      0      40      44     302    1395 
   Size:         40       4     258    1093

  Fragments arranged by size: 

                1093     258      40       4
 
EagI C'GGCC_G

Cuts at:      0      40      44    1395 
   Size:         40       4    1351

  Fragments arranged by size: 

                1351      40       4
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     435     826    1395 
   Size:        435     391     569

  Fragments arranged by size: 

                 569     435     391
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     838    1160    1291    1395 
   Size:        838     322     131     104

  Fragments arranged by size: 

                 838     322     131     104
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      97     436    1395 
   Size:         97     339     959

  Fragments arranged by size: 

                 959     339      97
 
EcoO109I rG'GnC_Cy

Cuts at:      0     793    1395 
   Size:        793     602
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1080    1395 
   Size:       1080     315
 
FseI GG_CCGG'CC

Cuts at:      0      46    1395 
   Size:         46    1349
 
FspI TGC'GCA

Cuts at:      0     313    1395 
   Size:        313    1082
 
HaeII r_GCGC'y

Cuts at:      0      69     906    1345    1395 
   Size:         69     837     439      50

  Fragments arranged by size: 

                 837     439      69      50
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     572     604    1395 
   Size:        572      32     791

  Fragments arranged by size: 

                 791     572      32
 
KasI G'GCGC_C

Cuts at:      0      65    1341    1395 
   Size:         65    1276      54

  Fragments arranged by size: 

                1276      65      54
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     416    1395 
   Size:        416     979
 
MscI TGG'CCA

Cuts at:      0     304    1395 
   Size:        304    1091
 
MspA1I CmG'CkG

Cuts at:      0      39     985    1004    1125    1395 
   Size:         39     946      19     121     270

  Fragments arranged by size: 

                 946     270     121      39      19
 
NaeI GCC'GGC

Cuts at:      0      44    1395 
   Size:         44    1351
 
NarI GG'CG_CC

Cuts at:      0      66    1342    1395 
   Size:         66    1276      53

  Fragments arranged by size: 

                1276      66      53
 
NgoMIV G'CCGG_C

Cuts at:      0      42    1395 
   Size:         42    1353
 
PasI CC'CwG_GG

Cuts at:      0     855    1395 
   Size:        855     540
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     985    1007    1395 
   Size:        985      22     388

  Fragments arranged by size: 

                 985     388      22
 
PfoI T'CCnGG_A

Cuts at:      0     710    1395 
   Size:        710     685
 
PspOMI G'GGCC_C

Cuts at:      0     793    1395 
   Size:        793     602
 
PstI C_TGCA'G

Cuts at:      0     176    1072    1395 
   Size:        176     896     323

  Fragments arranged by size: 

                 896     323     176
 
PvuII CAG'CTG

Cuts at:      0     985    1395 
   Size:        985     410
 
SacII CC_GC'GG

Cuts at:      0      40    1395 
   Size:         40    1355
 
SbfI CC_TGCA'GG

Cuts at:      0     176    1395 
   Size:        176    1219
 
ScaI AGT'ACT

Cuts at:      0     193     507    1395 
   Size:        193     314     888

  Fragments arranged by size: 

                 888     314     193
 
SexAI A'CCwGG_T

Cuts at:      0     160     464     845    1395 
   Size:        160     304     381     550

  Fragments arranged by size: 

                 550     381     304     160
 
SfcI C'TryA_G

Cuts at:      0     172    1068    1395 
   Size:        172     896     327

  Fragments arranged by size: 

                 896     327     172
 
SfoI GGC'GCC

Cuts at:      0      67    1343    1395 
   Size:         67    1276      52

  Fragments arranged by size: 

                1276      67      52
 
SmaI CCC'GGG

Cuts at:      0      63     963    1162    1380    1395 
   Size:         63     900     199     218      15

  Fragments arranged by size: 

                 900     218     199      63      15
 
SmlI C'TyrA_G

Cuts at:      0     702    1395 
   Size:        702     693
 
SrfI GCCC'GGGC

Cuts at:      0      63    1395 
   Size:         63    1332
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     112    1129    1395 
   Size:        112    1017     266

  Fragments arranged by size: 

                1017     266     112
 
TatI w'GTAC_w

Cuts at:      0      77     191     505    1395 
   Size:         77     114     314     890

  Fragments arranged by size: 

                 890     314     114      77
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     864     896    1174    1206    1395 
   Size:        864      32     278      32     189

  Fragments arranged by size: 

                 864     278     189      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     466    1395 
   Size:        466     929
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     804    1395 
   Size:        804     591
 
XmaI C'CCGG_G

Cuts at:      0      61     961    1160    1378    1395 
   Size:         61     900     199     218      17

  Fragments arranged by size: 

                 900     218     199      61      17
 

 Enzymes that do cut:

     AcuI    AflII   AflIII     AgeI     AlfI     AloI    AlwNI     ApaI
     AvaI    BamHI     BanI    BanII     BbeI     BcgI     BglI Bme1580I
    BmgBI     BpmI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspMI    BsrBI
    BsrFI   BstEII    BstXI    BstYI     BtgI     BtsI     ClaI     EaeI
     EagI     EciI  Eco57MI    EcoNI EcoO109I     FalI     FseI     FspI
    HaeII    Hin4I     KasI     MmeI     MscI   MspA1I     NaeI     NarI
   NgoMIV     PasI    PflMI     PfoI   PspOMI     PstI    PvuII    SacII
     SbfI     ScaI    SexAI     SfcI     SfoI     SmaI     SmlI     SrfI
    TaqII     TatI     TstI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI     AhdI     AleI
    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI     BbsI
    BbvCI    BciVI     BclI    BfrBI    BglII     BlpI     BmrI     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsiWI    BsmBI    BspHI
    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI  BstZ17I   Bsu36I
    BtgZI    CspCI     DraI   DraIII     DrdI     EarI  EcoICRI    EcoRI
    EcoRV    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MslI     NcoI     NdeI     NheI     NotI     NruI     NsiI     NspI
     PacI     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
    PspXI     PsrI     PvuI    RsrII     SacI     SalI    SanDI     SapI
     SfiI     SgfI    SgrAI    SnaBI     SpeI     SphI     SspI     StuI
     StyI     SwaI     XbaI     XhoI     XmnI     ZraI
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