Restriction map for pF1KB9448
##################################
# NO CUT ENZYMES (  134 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AviII       AvrII       BamHI       
BanI        BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfrI        BglI        
BglII       BlnI        BpmI        BpuAI       BsaAI       
BsaHI       BsaI        BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       Bsp1407I    BspDI       BspEI       
BspLU11I    BspMI       BsrFI       BsrGI       BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstYI       BstZ17I     Bsu36I      Cfr10I      ClaI        
CpoI        DraIII      DrdI        DsaI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        Hin1I       HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MaeII       MfeI        MflI        MluI        MroI        
MunI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NotI        NruI        NsiI        NspV        
PacI        PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RsaI        
RsrII       SacII       SalI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SnaBI       SpeI        Sse8387I    SspBI       StuI        
SwaI        XbaI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccII         543  CGCG                 CG^CG
AcsI          743  AAATTC               [GA]^AATT[CT]
ApoI          743  AAATTC               [GA]^AATT[CT]
AspI          220  GACTGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaII          20  GGACC                G^G[AT]CC
BalI          683  TGGCCA               TGG^CCA
BlpI         1332  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1332  GCTGAGC              GC^T[ACGT]AGC
BsaBI         718  GATGATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI          406  GTGCAG               GTGCAG(16/14)
BsmI         1280  GAATGC               GAATGC(1/-1)
BspHI         813  TCATGA               T^CATGA
BsrBI         610  GAGCGG               CCGCTC(-3/-3)
BsrDI         353  GCAATG               GCAATG(2/0)
BssSI         163  CTCGTG               CACGAG(-5/-1)
BstUI         543  CGCG                 CG^CG
CelII        1332  GCTGAGC              GC^T[ACGT]AGC
DraII          19  AGGACCC              [GA]G^G[ACGT]CC[CT]
EaeI          683  TGGCCA               [CT]^GGCC[GA]
EcoNI          11  CCTTACCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I       19  AGGACCC              [GA]G^G[ACGT]CC[CT]
FauI          541  CCCGC                CCCGC(4/6)
HaeII         567  GGCGCT               [GA]GCGC^[CT]
HapII         360  CCGG                 C^CGG
HgaI          544  GCGTC                GACGC(5/10)
HpaII         360  CCGG                 C^CGG
MamI          718  GATGATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         683  TGGCCA               TGG^CCA
MscI          683  TGGCCA               TGG^CCA
MspA1I         37  CAGCTG               C[AC]G^C[GT]G
MspI          360  CCGG                 C^CGG
MvnI          543  CGCG                 CG^CG
NheI          175  GCTAGC               G^CTAGC
NspI         1164  GCATGC               [GA]CATG^[CT]
PaeR7I        914  CTCGAG               C^TCGAG
PflFI         220  GACTGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         473  CCAGACTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI          19  AGGACCC              [GA]G^G[AT]CC[CT]
PvuII          37  CAGCTG               CAG^CTG
RcaI          813  TCATGA               T^CATGA
SapI         1210  GAAGAGC              GCTCTTC(1/4)
SmaI          359  CCCGGG               CCC^GGG
SmlI          914  CTCGAG               C^T[CT][GA]AG
SphI         1164  GCATGC               GCATG^C
SspI           48  AATATT               AAT^ATT
Tth111I       220  GACTGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        473  CCAGACTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          671  CCAACAAGAAGTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          914  CTCGAG               C^TCGAG
XmaCI         359  CCCGGG               C^CCGGG
XmaI          359  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AlwI          724  GATCC                GGATC(4/5)
              304  GGATC               
AvaI          359  CCCGGG               C^[CT]CG[GA]G
              914  CTCGAG              
BcnI          359  CCCGG                CC^[GC]GG
              360  CCGGG               
BsmAI         218  GAGAC                GTCTC(1/5)
             1155  GAGAC               
BsoBI         359  CCCGGG               C^[CT]CG[GA]G
              914  CTCGAG              
BsrI          221  ACTGG                ACTGG(1/-1)
             1349  CCAGT               
BstXI          17  CCAGGACCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
               93  CCAACAAGGTGG        
CfoI          568  GCGC                 GCG^C
              586  GCGC                
Cfr13I         20  GGACC                G^G[ACGT]CC
              618  GGCCC               
DpnII         305  GATC                 ^GATC
              724  GATC                
DraI          372  TTTAAA               TTT^AAA
             1389  TTTAAA              
EcoT14I       896  CCTTGG               C^C[AT][AT]GG
             1380  CCTTGG              
HhaI          568  GCGC                 GCG^C
              586  GCGC                
HinP1I        568  GCGC                 G^CGC
              586  GCGC                
MboI          305  GATC                 ^GATC
              724  GATC                
MslI          308  CACCAACATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              399  CACCTGTGTG          
NciI          359  CCCGG                CC^[GC]GG
              360  CCGGG               
NdeII         305  GATC                 ^GATC
              724  GATC                
SacI          817  GAGCTC               GAGCT^C
             1203  GAGCTC              
Sau3AI        305  GATC                 ^GATC
              724  GATC                
Sau96I         20  GGACC                G^G[ACGT]CC
              618  GGCCC               
SfaNI          82  GATGC                GCATC(5/9)
              574  GATGC               
StyI          896  CCTTGG               C^C[AT][AT]GG
             1380  CCTTGG              
TaqI          903  TCGA                 T^CGA
              915  TCGA                
TthHB8I       903  TCGA                 T^CGA
              915  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   48 enzymes) #
#########################################

AciI        AluI        AlwNI       AspHI       BanII       
BbvI        BfaI        BmyI        BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmFI       Bsp1286I    
BssKI       BstF5I      BstNI       Cac8I       DdeI        
EarI        EcoRII      Fnu4HI      FokI        HaeIII      
HinfI       HphI        ItaI        Ksp632I     MaeI        
MaeIII      MboII       MnlI        MseI        MvaI        
MwoI        NlaIII      NlaIV       PleI        PstI        
ScrFI       SfcI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       
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