Restriction map for pF1KB9443
##################################
# NO CUT ENZYMES (  145 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        BalI        
BamHI       BanI        BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BfrI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaI        BseAI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspLU11I    BspMI       BsrDI       BsrGI       
BssHII      Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstXI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        Eam1105I    Eco47III    Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MamI        
MfeI        MluI        MluNI       MroI        MscI        
MseI        MspA1I      MunI        NarI        NcoI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
PvuII       RsrII       SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfoI        SfuI        
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        TaqI        Tru9I       
Tsp509I     Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccII          60  CGCG                 CG^CG
AvaI          342  CTCGGG               C^[CT]CG[GA]G
AviII         533  TGCGCA               TGC^GCA
AvrII         906  CCTAGG               C^CTAGG
BfaI          907  CTAG                 C^TAG
BglI          169  GCCGTGTCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         719  AGATCT               A^GATCT
BlnI          906  CCTAGG               C^CTAGG
BpmI          664  CTCCAG               CTGGAG(16/14)
BsaWI          32  ACCGGA               [AT]^CCGG[AT]
BsiEI          83  CGGCCG               CG[GA][CT]^CG
BsmAI          21  GTCTC                GTCTC(1/5)
BsoBI         342  CTCGGG               C^[CT]CG[GA]G
BspHI         800  TCATGA               T^CATGA
BsrI          443  CCAGT                ACTGG(1/-1)
BssSI         658  CACGAG               CACGAG(-5/-1)
BstUI          60  CGCG                 CG^CG
BstYI         719  AGATCT               [GA]^GATC[CT]
DdeI          369  CTCAG                C^T[ACGT]AG
EagI           83  CGGCCG               C^GGCCG
EarI          156  GAAGAG               CTCTTC(1/4)
EclXI          83  CGGCCG               C^GGCCG
Eco52I         83  CGGCCG               C^GGCCG
FauI          729  CCCGC                CCCGC(4/6)
FspI          533  TGCGCA               TGC^GCA
HaeII         125  AGCGCC               [GA]GCGC^[CT]
HinfI         161  GAATC                G^A[ACGT]TC
Ksp632I       156  GAAGAG               CTCTTC(1/4)
MaeI          907  CTAG                 C^TAG
MaeII         599  ACGT                 A^CGT
MaeIII        397  GTCAC                ^GT[ACGT]AC
MflI          719  AGATCT               [GA]^GATC[CT]
MslI          138  CATCTCCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI           60  CGCG                 CG^CG
NaeI          591  GCCGGC               GCC^GGC
NgoMIV        591  GCCGGC               G^CCGGC
RcaI          800  TCATGA               T^CATGA
SfaNI         813  GATGC                GCATC(5/9)
SfiI          168  GGCCGTGTCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI         481  CGCCGGTG             C[GA]^CCGG[CT]G
SmlI          733  CTCAAG               C^T[CT][GA]AG
TfiI          161  GAATC                G^A[AT]TC
Tsp45I        397  GTCAC                ^GT[GC]AC
TspRI         444  CAGTG                CA[GC]TG[ACGT][ACGT]^
XhoII         719  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AcyI          411  GACGCC               G[GA]^CG[CT]C
              435  GGCGTC              
AfaI           88  GTAC                 GT^AC
              690  GTAC                
AlwI          654  GGATC                GGATC(4/5)
              915  GGATC               
BbsI          604  GTCTTC               GAAGAC(2/6)
              853  GTCTTC              
BcnI          327  CCCGG                CC^[GC]GG
              356  CCGGG               
BpuAI         604  GTCTTC               GAAGAC(2/6)
              853  GTCTTC              
BsaHI         411  GACGCC               G[GA]^CG[CT]C
              435  GGCGTC              
BsgI          320  CTGCAC               GTGCAG(16/14)
              202  GTGCAG              
BstF5I         54  GGATG                GGATG(2/0)
              627  GGATG               
DsaI           68  CCGTGG               C^C[GA][CT]GG
              512  CCACGG              
FokI           54  GGATG                GGATG(9/13)
              627  GGATG               
HgaI          411  GACGC                GACGC(5/10)
              436  GCGTC               
Hin1I         411  GACGCC               G[GA]^CG[CT]C
              435  GGCGTC              
NciI          327  CCCGG                CC^[GC]GG
              356  CCGGG               
NspI          614  ACATGC               [GA]CATG^[CT]
              682  ACATGC              
RsaI           88  GTAC                 GT^AC
              690  GTAC                
SacI          661  GAGCTC               GAGCT^C
              804  GAGCTC              
StuI          764  AGGCCT               AGG^CCT
              909  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (   50 enzymes) #
#########################################

AciI        AluI        AspHI       AvaII       BanII       
BbvI        BmyI        BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmFI       Bsp1286I    BsrBI       
BsrFI       BssKI       BstNI       Cac8I       CfoI        
Cfr10I      Cfr13I      DpnII       EaeI        EcoRII      
EcoT14I     Fnu4HI      HaeIII      HapII       HhaI        
HinP1I      HpaII       HphI        ItaI        MboI        
MboII       MnlI        MspI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       PstI        Sau3AI      
Sau96I      ScrFI       SfcI        StyI        TseI        
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