Restriction map for pF1KB9443
 (Linear) (Six-base) MAPSORT of: KIBB9443.seq  Check: 9963  from: 1  to: 921



KIBB9443.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:38  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     142     277     517     886     921 
   Size:        142     135     240     369      35

  Fragments arranged by size: 

                 369     240     142     135      35
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     842     921 
   Size:        842      79
 
AvaI C'yCGr_G

Cuts at:      0     342     921 
   Size:        342     579
 
AvrII C'CTAG_G

Cuts at:      0     906     921 
   Size:        906      15
 
BanII G_rGCy'C

Cuts at:      0     364     652     665     808     921 
   Size:        364     288      13     143     113

  Fragments arranged by size: 

                 364     288     143     113      13
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     597     846     921 
   Size:        597     249      75

  Fragments arranged by size: 

                 597     249      75
 
BglI GCCn_nnn'nGGC

Cuts at:      0     175     921 
   Size:        175     746
 
BglII A'GATC_T

Cuts at:      0     719     921 
   Size:        719     202
 
Bme1580I G_kGCm'C

Cuts at:      0       7     921 
   Size:          7     914
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     666     921 
   Size:        666     255
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     649     921 
   Size:        649     272
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     718     921 
   Size:        718     203
 
BsaHI Gr'CG_yC

Cuts at:      0     412     436     921 
   Size:        412      24     485

  Fragments arranged by size: 

                 485     412      24
 
BsaWI w'CCGG_w

Cuts at:      0      32     921 
   Size:         32     889
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     706     736     921 
   Size:        706      30     185

  Fragments arranged by size: 

                 706     185      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     354     357     627     892     921 
   Size:        354       3     270     265      29

  Fragments arranged by size: 

                 354     270     265      29       3
 
BseYI C'CCAG_C

Cuts at:      0      25     296     311     921 
   Size:         25     271      15     610

  Fragments arranged by size: 

                 610     271      25      15
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     223     305     921 
   Size:        223      82     616

  Fragments arranged by size: 

                 616     223      82
 
BsiEI CG_ry'CG

Cuts at:      0      86     921 
   Size:         86     835
 
BsiHKAI G_wGCw'C

Cuts at:      0     527     665     808     921 
   Size:        527     138     143     113

  Fragments arranged by size: 

                 527     143     138     113
 
Bsp1286I G_dGCh'C

Cuts at:      0       7     364     527     652     665     808     921 
   Size:          7     357     163     125      13     143     113

  Fragments arranged by size: 

                 357     163     143     125     113      13       7
 
BspHI T'CATG_A

Cuts at:      0     800     921 
   Size:        800     121
 
BsrBI CCG'CTC

Cuts at:      0     548     732     878     921 
   Size:        548     184     146      43

  Fragments arranged by size: 

                 548     184     146      43
 
BsrFI r'CCGG_y

Cuts at:      0     306     482     591     921 
   Size:        306     176     109     330

  Fragments arranged by size: 

                 330     306     176     109
 
BssSI C'ACGA_G

Cuts at:      0     658     921 
   Size:        658     263
 
BstYI r'GATC_y

Cuts at:      0     719     921 
   Size:        719     202
 
BtgI C'CryG_G

Cuts at:      0      68     512     921 
   Size:         68     444     409

  Fragments arranged by size: 

                 444     409      68
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      30      65     921 
   Size:         30      35     856

  Fragments arranged by size: 

                 856      35      30
 
EaeI y'GGCC_r

Cuts at:      0      62      83     167     176     329     593     879     921 
   Size:         62      21      84       9     153     264     286      42

  Fragments arranged by size: 

                 286     264     153      84      62      42      21       9
            
 
EagI C'GGCC_G

Cuts at:      0      83     921 
   Size:         83     838
 
EarI CTCTTCn'nnn_

Cuts at:      0     151     921 
   Size:        151     770
 
EcoICRI GAG'CTC

Cuts at:      0     663     806     921 
   Size:        663     143     115

  Fragments arranged by size: 

                 663     143     115
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     142     277     517     649     886     921 
   Size:        142     135     240     132     237      35

  Fragments arranged by size: 

                 240     237     142     135     132      35
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     783     921 
   Size:        783     138
 
FspI TGC'GCA

Cuts at:      0     535     921 
   Size:        535     386
 
HaeII r_GCGC'y

Cuts at:      0     129     921 
   Size:        129     792
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     619     651     921 
   Size:        619      32     270

  Fragments arranged by size: 

                 619     270      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     884     921 
   Size:        884      37
 
MslI CAynn'nnrTG

Cuts at:      0     142     921 
   Size:        142     779
 
NaeI GCC'GGC

Cuts at:      0     593     921 
   Size:        593     328
 
NgoMIV G'CCGG_C

Cuts at:      0     591     921 
   Size:        591     330
 
NspI r_CATG'y

Cuts at:      0     618     686     921 
   Size:        618      68     235

  Fragments arranged by size: 

                 618     235      68
 
PstI C_TGCA'G

Cuts at:      0     563     625     821     921 
   Size:        563      62     196     100

  Fragments arranged by size: 

                 563     196     100      62
 
SacI G_AGCT'C

Cuts at:      0     665     808     921 
   Size:        665     143     113

  Fragments arranged by size: 

                 665     143     113
 
SfcI C'TryA_G

Cuts at:      0     559     621     817     921 
   Size:        559      62     196     104

  Fragments arranged by size: 

                 559     196     104      62
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     175     921 
   Size:        175     746
 
SgrAI Cr'CCGG_yG

Cuts at:      0     482     921 
   Size:        482     439
 
SmlI C'TyrA_G

Cuts at:      0     733     921 
   Size:        733     188
 
StuI AGG'CCT

Cuts at:      0     766     911     921 
   Size:        766     145      10

  Fragments arranged by size: 

                 766     145      10
 
StyI C'CwwG_G

Cuts at:      0     107     151     906     921 
   Size:        107      44     755      15

  Fragments arranged by size: 

                 755     107      44      15
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     454     921 
   Size:        454     467
 

 Enzymes that do cut:

     AcuI     AlfI     AvaI    AvrII    BanII     BbsI     BglI    BglII
 Bme1580I     BplI     BpmI    BpuEI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspHI    BsrBI    BsrFI
    BssSI    BstYI     BtgI    CspCI     EaeI     EagI     EarI  EcoICRI
  Eco57MI     FalI     FspI    HaeII    Hin4I     MmeI     MslI     NaeI
   NgoMIV     NspI     PstI     SacI     SfcI     SfiI    SgrAI     SmlI
     StuI     StyI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     BaeI    BamHI     BanI     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BlpI    BmgBI     BmrI     BmtI
   Bpu10I     BsaI    BsaAI    BsaBI    BsiWI     BsmI    BsmBI    BspEI
    BspMI    BsrDI    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI     DraI   DraIII     DrdI
     EciI    EcoNI EcoO109I    EcoRI    EcoRV     FseI    FspAI   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI   MspA1I
     NarI     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfoI
     SgfI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI
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