Restriction map for pF1KB9423
##################################
# NO CUT ENZYMES (  147 enzymes) #
##################################

AatII       Acc65I      AcsI        AflII       AflIII      
AgeI        AhdI        AlwNI       AocI        ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AviII       AvrII       BalI        
BamHI       BbeI        BbvCI       BcgI        BciVI       
BclI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BsaBI       BsaI        
BseRI       BsiEI       BsiWI       BsmAI       BsmBI       
BsmFI       BsmI        Bsp1407I    BspDI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DdeI        DraI        DrdI        
EagI        Eam1105I    EclXI       Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FauI        FbaI        FseI        FspI        HindIII     
HinfI       HpaI        HphI        KasI        KpnI        
KspI        MaeI        MaeIII      MamI        MfeI        
MluI        MluNI       MscI        MslI        MunI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmeI        PshAI       PshBI       
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TfiI        Tsp45I      
Tsp509I     Tth111I     Van91I      XbaI        XcmI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccI          496  GTCTAC               GT^[AC][GT]AC
AccIII        320  TCCGGA               T^CCGGA
AclI          415  AACGTT               AA^CGTT
AcyI          108  GGCGTC               G[GA]^CG[CT]C
Alw44I        490  GTGCAC               G^TGCAC
Aor51HI        80  AGCGCT               AGC^GCT
ApaI          279  GGGCCC               GGGCC^C
ApaLI         490  GTGCAC               G^TGCAC
AspHI         490  GTGCAC               G[AT]GC[AT]^C
AvaI          156  CCCGGG               C^[CT]CG[GA]G
BbsI           25  GTCTTC               GAAGAC(2/6)
BpmI          613  CTGGAG               CTGGAG(16/14)
BpuAI          25  GTCTTC               GAAGAC(2/6)
BsaHI         108  GGCGTC               G[GA]^CG[CT]C
BsaWI         320  TCCGGA               [AT]^CCGG[AT]
BseAI         320  TCCGGA               T^CCGGA
BsiHKAI       490  GTGCAC               G[AT]GC[AT]^C
BsoBI         156  CCCGGG               C^[CT]CG[GA]G
BspEI         320  TCCGGA               T^CCGGA
BstAPI        447  GCAGACGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI         347  CCAGG                CC^[AT]GG
BstYI         461  AGATCC               [GA]^GATC[CT]
DraIII        190  CACGTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          329  CCACGG               C^C[GA][CT]GG
EaeI          140  TGGCCG               [CT]^GGCC[GA]
EarI          403  CTCTTC               CTCTTC(1/4)
Eco47III       80  AGCGCT               AGC^GCT
EcoRII        347  CCAGG                ^CC[AT]GG
EcoT14I       605  CCAAGG               C^C[AT][AT]GG
HaeII          80  AGCGCT               [GA]GCGC^[CT]
Hin1I         108  GGCGTC               G[GA]^CG[CT]C
HincII        214  GTCAAC               GT[CT]^[GA]AC
HindII        214  GTCAAC               GT[CT]^[GA]AC
Ksp632I       403  CTCTTC               CTCTTC(1/4)
MflI          461  AGATCC               [GA]^GATC[CT]
MroI          320  TCCGGA               T^CCGGA
MseI          206  TTAA                 T^TAA
MvaI          347  CCAGG                CC^[AT]GG
Psp1406I      415  AACGTT               AA^CGTT
PstI          445  CTGCAG               CTGCA^G
PvuII         148  CAGCTG               CAG^CTG
SfaNI         518  GCATC                GCATC(5/9)
SfcI          445  CTGCAG               C^T[GA][CT]AG
SmaI          156  CCCGGG               CCC^GGG
StyI          605  CCAAGG               C^C[AT][AT]GG
Tru9I         206  TTAA                 T^TAA
XhoII         461  AGATCC               [GA]^GATC[CT]
XmaCI         156  CCCGGG               C^CCGGG
XmaI          156  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AfaI          294  GTAC                 GT^AC
              351  GTAC                
AlwI          462  GATCC                GGATC(4/5)
              374  GGATC               
BanI           73  GGCACC               G^G[CT][GA]CC
              229  GGCACC              
BanII         279  GGGCCC               G[GA]GC[CT]^C
              531  GGGCTC              
BbrPI         190  CACGTG               CAC^GTG
              544  CACGTG              
BsaAI         190  CACGTG               [CT]AC^GT[GA]
              544  CACGTG              
BsrI          176  ACTGG                ACTGG(1/-1)
              371  ACTGG               
BstXI         347  CCAGGTACATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              605  CCAAGGAGCTGG        
HgaI          109  GCGTC                GACGC(5/10)
              212  GCGTC               
MspA1I        148  CAGCTG               C[AC]G^C[GT]G
              297  CCGCTG              
NlaIII        270  CATG                 CATG^
              354  CATG                
PmaCI         190  CACGTG               CAC^GTG
              544  CACGTG              
PmlI          190  CACGTG               CAC^GTG
              544  CACGTG              
PpuMI         164  AGGACCT              [GA]G^G[AT]CC[CT]
              398  AGGACCT             
RsaI          294  GTAC                 GT^AC
              351  GTAC                
TspRI         335  CACTG                CA[GC]TG[ACGT][ACGT]^
              620  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (   46 enzymes) #
#########################################

AccII       AciI        AluI        AvaII       BbvI        
BcnI        BmyI        BsaJI       BsgI        BsiYI       
BslI        Bsp1286I    BspMI       BssKI       BstF5I      
BstUI       Cac8I       CfoI        Cfr13I      DpnII       
DraII       EcoO109I    Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HpaII       ItaI        
MaeII       MboI        MboII       MnlI        MspI        
MvnI        MwoI        NciI        NdeII       NlaIV       
Sau3AI      Sau96I      ScrFI       TaqI        TseI        
TthHB8I     
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