Restriction map for pF1KB9423
 (Linear) (Six-base) MAPSORT of: KIBB9423.seq  Check: 5199  from: 1  to: 651



KIBB9423.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:38  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     562     651 
   Size:        562      89
 
AccI GT'mk_AC

Cuts at:      0     497     651 
   Size:        497     154
 
AclI AA'CG_TT

Cuts at:      0     416     651 
   Size:        416     235
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      37     651 
   Size:         37     614
 
AfeI AGC'GCT

Cuts at:      0      82     651 
   Size:         82     569
 
ApaI G_GGCC'C

Cuts at:      0     283     651 
   Size:        283     368
 
ApaLI G'TGCA_C

Cuts at:      0     490     651 
   Size:        490     161
 
AvaI C'yCGr_G

Cuts at:      0     156     651 
   Size:        156     495
 
BanI G'GyrC_C

Cuts at:      0      73     229     651 
   Size:         73     156     422

  Fragments arranged by size: 

                 422     156      73
 
BanII G_rGCy'C

Cuts at:      0     283     535     651 
   Size:        283     252     116

  Fragments arranged by size: 

                 283     252     116
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      18     651 
   Size:         18     633
 
Bme1580I G_kGCm'C

Cuts at:      0      76     283     494     651 
   Size:         76     207     211     157

  Fragments arranged by size: 

                 211     207     157      76
 
BmgBI CAC'GTC

Cuts at:      0     495     651 
   Size:        495     156
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     186     651 
   Size:        186     465
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     634     651 
   Size:        634      17
 
BsaAI yAC'GTr

Cuts at:      0     192     546     651 
   Size:        192     354     105

  Fragments arranged by size: 

                 354     192     105
 
BsaHI Gr'CG_yC

Cuts at:      0     109     651 
   Size:        109     542
 
BsaWI w'CCGG_w

Cuts at:      0     320     651 
   Size:        320     331
 
BseYI C'CCAG_C

Cuts at:      0      77     651 
   Size:         77     574
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      76     166     231     651 
   Size:         76      90      65     420

  Fragments arranged by size: 

                 420      90      76      65
 
BsiHKAI G_wGCw'C

Cuts at:      0     494     651 
   Size:        494     157
 
Bsp1286I G_dGCh'C

Cuts at:      0      76     283     494     535     651 
   Size:         76     207     211      41     116

  Fragments arranged by size: 

                 211     207     116      76      41
 
BspEI T'CCGG_A

Cuts at:      0     320     651 
   Size:        320     331
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     140     368     562     651 
   Size:        140     228     194      89

  Fragments arranged by size: 

                 228     194     140      89
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     453     651 
   Size:        453     198
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     354     612     651 
   Size:        354     258      39

  Fragments arranged by size: 

                 354     258      39
 
BstYI r'GATC_y

Cuts at:      0     461     651 
   Size:        461     190
 
BtgI C'CryG_G

Cuts at:      0     329     651 
   Size:        329     322
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     504     651 
   Size:        504     147
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     399     434     651 
   Size:        399      35     217

  Fragments arranged by size: 

                 399     217      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     195     651 
   Size:        195     456
 
EaeI y'GGCC_r

Cuts at:      0     140     651 
   Size:        140     511
 
EarI CTCTTCn'nnn_

Cuts at:      0     409     651 
   Size:        409     242
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      37     651 
   Size:         37     614
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      37     634     651 
   Size:         37     597      17

  Fragments arranged by size: 

                 597      37      17
 
EcoO109I rG'GnC_Cy

Cuts at:      0     165     279     280     399     651 
   Size:        165     114       1     119     252

  Fragments arranged by size: 

                 252     165     119     114       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      42     651 
   Size:         42     609
 
HaeII r_GCGC'y

Cuts at:      0      84     651 
   Size:         84     567
 
HincII GTy'rAC

Cuts at:      0     216     651 
   Size:        216     435
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      29     651 
   Size:         29     622
 
MspA1I CmG'CkG

Cuts at:      0     150     299     651 
   Size:        150     149     352

  Fragments arranged by size: 

                 352     150     149
 
PmlI CAC'GTG

Cuts at:      0     192     546     651 
   Size:        192     354     105

  Fragments arranged by size: 

                 354     192     105
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     301     333     651 
   Size:        301      32     318

  Fragments arranged by size: 

                 318     301      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     165     399     651 
   Size:        165     234     252

  Fragments arranged by size: 

                 252     234     165
 
PspOMI G'GGCC_C

Cuts at:      0     279     651 
   Size:        279     372
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     541     573     651 
   Size:        541      32      78

  Fragments arranged by size: 

                 541      78      32
 
PstI C_TGCA'G

Cuts at:      0     449     651 
   Size:        449     202
 
PvuII CAG'CTG

Cuts at:      0     150     651 
   Size:        150     501
 
SfcI C'TryA_G

Cuts at:      0     445     651 
   Size:        445     206
 
SmaI CCC'GGG

Cuts at:      0     158     651 
   Size:        158     493
 
StyI C'CwwG_G

Cuts at:      0     605     651 
   Size:        605      46
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      91     168     651 
   Size:         91      77     483

  Fragments arranged by size: 

                 483      91      77
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     414     446     651 
   Size:        414      32     205

  Fragments arranged by size: 

                 414     205      32
 
XmaI C'CCGG_G

Cuts at:      0     156     651 
   Size:        156     495
 

 Enzymes that do cut:

     AarI     AccI     AclI     AcuI     AfeI     ApaI    ApaLI     AvaI
     BanI    BanII     BbsI Bme1580I    BmgBI     BmrI     BpmI    BsaAI
    BsaHI    BsaWI    BseYI     BsgI  BsiHKAI Bsp1286I    BspEI    BspMI
   BstAPI    BstXI    BstYI     BtgI    BtgZI    CspCI   DraIII     EaeI
     EarI     EciI  Eco57MI EcoO109I     FalI    HaeII   HincII     MmeI
   MspA1I     PmlI     PpiI    PpuMI   PspOMI     PsrI     PstI    PvuII
     SfcI     SmaI     StyI    TaqII     TstI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I    AflII   AflIII     AgeI     AhdI     AleI     AlfI
     AloI    AlwNI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaBI
    BsaXI    BseRI    BsiEI    BsiWI     BsmI    BsmBI    BspHI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII  BstZ17I
   Bsu36I     BtsI     ClaI     DraI     DrdI     EagI  EcoICRI    EcoNI
    EcoRI    EcoRV     FseI     FspI    FspAI    Hin4I  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     MslI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI    PshAI     PsiI
    PspXI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI
  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI
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