Restriction map for pF1KB9340
##################################
# NO CUT ENZYMES (  117 enzymes) #
##################################

Acc65I      AccI        AccII       AccIII      AclI        
AgeI        AhdI        AocI        Aor51HI     ApaI        
AscI        AseI        AsnI        Asp718I     AspEI       
AvaI        AvrII       BbeI        BbrPI       BbvCI       
BciVI       BlnI        BsaAI       BsaI        BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       BsmFI       
BsoBI       BspEI       BspHI       BsrBI       BsrFI       
BssHII      BssSI       Bst1107I    BstEII      BstPI       
BstUI       BstZ17I     Bsu36I      Cfr10I      CpoI        
DraI        DrdI        DsaI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FauI        FseI        HaeII       HgaI        HpaI        
KasI        KpnI        Ksp632I     KspI        MluI        
MroI        MslI        MvnI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        PacI        PaeR7I      PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SfiI        SfoI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
StuI        SwaI        Van91I      XbaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AatII        2044  GACGTC               GACGT^C
AciI         1050  GCGG                 CCGC(-3/-1)
AcyI         2044  GACGTC               G[GA]^CG[CT]C
AlwNI         410  CAGCTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspI         2133  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        1515  TGCGCA               TGC^GCA
BalI          266  TGGCCA               TGG^CCA
BamHI        1667  GGATCC               G^GATCC
BbsI         1240  GAAGAC               GAAGAC(2/6)
BcgI         1737  GCATCATTGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         2265  TGATCA               T^GATCA
BcnI         1058  CCCGG                CC^[GC]GG
BglI          213  GCCACAGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII          63  AGATCT               A^GATCT
BlpI         1639  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1639  GCTGAGC              GC^T[ACGT]AGC
BpuAI        1240  GAAGAC               GAAGAC(2/6)
BsaBI        2263  GATGATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        2044  GACGTC               G[GA]^CG[CT]C
BsgI         1938  GTGCAG               GTGCAG(16/14)
Bsp1407I     2021  TGTACA               T^GTACA
BspDI        1558  ATCGAT               AT^CGAT
BsrDI         925  GCAATG               GCAATG(2/0)
BsrGI        2021  TGTACA               T^GTACA
BstAPI       1026  GCATCACATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        1400  TTCGAA               TT^CGAA
BstXI        1470  CCAGGTGTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII        1639  GCTGAGC              GC^T[ACGT]AGC
ClaI         1558  ATCGAT               AT^CGAT
DraII        1541  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EaeI          266  TGGCCA               [CT]^GGCC[GA]
EcoO109I     1541  AGGCCCT              [GA]G^G[ACGT]CC[CT]
FbaI         2265  TGATCA               T^GATCA
FspI         1515  TGCGCA               TGC^GCA
HapII        1059  CCGG                 C^CGG
Hin1I        2044  GACGTC               G[GA]^CG[CT]C
HincII        664  GTCAAC               GT[CT]^[GA]AC
HindII        664  GTCAAC               GT[CT]^[GA]AC
HpaII        1059  CCGG                 C^CGG
MamI         2263  GATGATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1598  CAATTG               C^AATTG
MluNI         266  TGGCCA               TGG^CCA
MscI          266  TGGCCA               TGG^CCA
MspI         1059  CCGG                 C^CGG
MunI         1598  CAATTG               C^AATTG
NciI         1058  CCCGG                CC^[GC]GG
NspV         1400  TTCGAA               TT^CGAA
PflFI        2133  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PvuII         574  CAGCTG               CAG^CTG
SexAI        2229  ACCAGGT              A^CC[AT]GGT
SfuI         1400  TTCGAA               TT^CGAA
SspBI        2021  TGTACA               T^GTACA
SspI          766  AATATT               AAT^ATT
Tth111I      2133  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AflII         886  CTTAAG               C^TTAAG
             1075  CTTAAG              
AflIII       1346  ACATGT               A^C[GA][CT]GT
             2024  ACATGT              
Alw44I        251  GTGCAC               G^TGCAC
             2149  GTGCAC              
ApaLI         251  GTGCAC               G^TGCAC
             2149  GTGCAC              
AvaII         579  GGACC                G^G[AT]CC
             2236  GGTCC               
BanI          876  GGCACC               G^G[CT][GA]CC
             2032  GGTGCC              
BanII        1655  GAGCCC               G[GA]GC[CT]^C
             2051  GAGCCC              
BfrI          886  CTTAAG               C^TTAAG
             1075  CTTAAG              
BsmI          824  GAATGC               GAATGC(1/-1)
             2293  GAATGC              
BspLU11I     1346  ACATGT               A^CATGT
             2024  ACATGT              
BspMI          15  ACCTGC               ACCTGC(4/8)
             2111  ACCTGC              
Cac8I        1159  GCAAGC               GC[ACGT]^[ACGT]GC
             1503  GCAGGC              
CfoI         1516  GCGC                 GCG^C
             2105  GCGC                
DraIII        376  CACATAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2228  CACCAGGTG           
HhaI         1516  GCGC                 GCG^C
             2105  GCGC                
HinP1I       1516  GCGC                 G^CGC
             2105  GCGC                
HindIII       350  AAGCTT               A^AGCTT
             1982  AAGCTT              
MaeII         954  ACGT                 A^CGT
             2045  ACGT                
MaeIII       1684  GTGAC                ^GT[ACGT]AC
             2215  GTCAC               
MspA1I        574  CAGCTG               C[AC]G^C[GT]G
             1048  CAGCGG              
PstI         1501  CTGCAG               CTGCA^G
             2162  CTGCAG              
SmlI          886  CTTAAG               C^T[CT][GA]AG
             1075  CTTAAG              
Tsp45I       1684  GTGAC                ^GT[GC]AC
             2215  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   64 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        ApoI        
Asp700I     AspHI       BbvI        BfaI        BmyI        
BpmI        BsaJI       BseRI       BsiHKAI     BsiYI       
BslI        BsmAI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       BstYI       Cfr13I      DdeI        
DpnII       EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HinfI       HphI        ItaI        MaeI        
MboI        MboII       MflI        MnlI        MseI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
NspI        PleI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp509I     TspRI       
TthHB8I     XcmI        XhoII       XmnI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com