Restriction map for pF1KB9340
 (Linear) (Six-base) MAPSORT of: KIBB9340.seq  Check: 6272  from: 1  to: 2298



KIBB9340.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:38  ..

AatII G_ACGT'C

Cuts at:      0    2048    2298 
   Size:       2048     250
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     850    1295    1572    1933    2200    2298 
   Size:        850     445     277     361     267      98

  Fragments arranged by size: 

                 850     445     361     277     267      98
 
AflII C'TTAA_G

Cuts at:      0     886    1075    2298 
   Size:        886     189    1223

  Fragments arranged by size: 

                1223     886     189
 
AflIII A'CryG_T

Cuts at:      0    1346    2024    2298 
   Size:       1346     678     274

  Fragments arranged by size: 

                1346     678     274
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2174    2298 
   Size:       2174     124
 
AlwNI CAG_nnn'CTG

Cuts at:      0     415    2298 
   Size:        415    1883
 
ApaLI G'TGCA_C

Cuts at:      0     251    2149    2298 
   Size:        251    1898     149

  Fragments arranged by size: 

                1898     251     149
 
ApoI r'AATT_y

Cuts at:      0     796     986    1369    1390    1892    2298 
   Size:        796     190     383      21     502     406

  Fragments arranged by size: 

                 796     502     406     383     190      21
 
BamHI G'GATC_C

Cuts at:      0    1667    2298 
   Size:       1667     631
 
BanI G'GyrC_C

Cuts at:      0     876    2032    2298 
   Size:        876    1156     266

  Fragments arranged by size: 

                1156     876     266
 
BanII G_rGCy'C

Cuts at:      0    1659    2055    2298 
   Size:       1659     396     243

  Fragments arranged by size: 

                1659     396     243
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1247    2298 
   Size:       1247    1051
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1726    1760    2298 
   Size:       1726      34     538

  Fragments arranged by size: 

                1726     538      34
 
BclI T'GATC_A

Cuts at:      0    2265    2298 
   Size:       2265      33
 
BglI GCCn_nnn'nGGC

Cuts at:      0     219    2298 
   Size:        219    2079
 
BglII A'GATC_T

Cuts at:      0      63    2298 
   Size:         63    2235
 
BlpI GC'TnA_GC

Cuts at:      0    1640    2298 
   Size:       1640     658
 
Bme1580I G_kGCm'C

Cuts at:      0     255    1367    2037    2153    2298 
   Size:        255    1112     670     116     145

  Fragments arranged by size: 

                1112     670     255     145     116
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      44     481     957    2130    2298 
   Size:         44     437     476    1173     168

  Fragments arranged by size: 

                1173     476     437     168      44
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     458     589     835    2298 
   Size:        458     131     246    1463

  Fragments arranged by size: 

                1463     458     246     131
 
BsaBI GATnn'nnATC

Cuts at:      0    2267    2298 
   Size:       2267      31
 
BsaHI Gr'CG_yC

Cuts at:      0    2045    2298 
   Size:       2045     253
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     179     209     890     920    2298 
   Size:        179      30     681      30    1378

  Fragments arranged by size: 

                1378     681     179      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     187     322     325    2298 
   Size:        187     135       3    1973

  Fragments arranged by size: 

                1973     187     135       3
 
BseYI C'CCAG_C

Cuts at:      0     203    2298 
   Size:        203    2095
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1959    2298 
   Size:       1959     339
 
BsiHKAI G_wGCw'C

Cuts at:      0     255     494    2153    2298 
   Size:        255     239    1659     145

  Fragments arranged by size: 

                1659     255     239     145
 
BsmI GAATG_Cn'

Cuts at:      0     830    2298 
   Size:        830    1468
 
Bsp1286I G_dGCh'C

Cuts at:      0     255     494    1367    1659    2037    2055    2153    2298 
   Size:        255     239     873     292     378      18      98     145

  Fragments arranged by size: 

                 873     378     292     255     239     145      98      18
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      24    2120    2298 
   Size:         24    2096     178

  Fragments arranged by size: 

                2096     178      24
 
BsrDI GCAATG_nn'

Cuts at:      0     932    2298 
   Size:        932    1366
 
BsrGI T'GTAC_A

Cuts at:      0    2021    2298 
   Size:       2021     277
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1032    2298 
   Size:       1032    1266
 
BstBI TT'CG_AA

Cuts at:      0    1401    2298 
   Size:       1401     897
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1477    2298 
   Size:       1477     821
 
BstYI r'GATC_y

Cuts at:      0      63    1283    1667    2298 
   Size:         63    1220     384     631

  Fragments arranged by size: 

                1220     631     384      63
 
ClaI AT'CG_AT

Cuts at:      0    1559    2298 
   Size:       1559     739
 
DraIII CAC_nnn'GTG

Cuts at:      0     381    2233    2298 
   Size:        381    1852      65

  Fragments arranged by size: 

                1852     381      65
 
EaeI y'GGCC_r

Cuts at:      0     266    2298 
   Size:        266    2032
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     458     589     835     850    1295    1572    1933    2200 
   Size:        458     131     246      15     445     277     361     267

Cuts at:   2200    2298 
   Size:         98

  Fragments arranged by size: 

                 458     445     361     277     267     246     131      98
                  15
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1542    2298 
   Size:       1542     756
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1224    1813    2029    2221    2298 
   Size:       1224     589     216     192      77

  Fragments arranged by size: 

                1224     589     216     192      77
 
FspI TGC'GCA

Cuts at:      0    1517    2298 
   Size:       1517     781
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     647     662     679     694    1271    1303    1606    1638 
   Size:        647      15      17      15     577      32     303      32

Cuts at:   1638    1891    1923    2298 
   Size:        253      32     375

  Fragments arranged by size: 

                 647     577     375     303     253      32      32      32
                  17      15      15
 
HincII GTy'rAC

Cuts at:      0     666    2298 
   Size:        666    1632
 
HindIII A'AGCT_T

Cuts at:      0     350    1982    2298 
   Size:        350    1632     316

  Fragments arranged by size: 

                1632     350     316
 
MfeI C'AATT_G

Cuts at:      0    1598    2298 
   Size:       1598     700
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     689    1750    1805    2298 
   Size:        689    1061      55     493

  Fragments arranged by size: 

                1061     689     493      55
 
MscI TGG'CCA

Cuts at:      0     268    2298 
   Size:        268    2030
 
MspA1I CmG'CkG

Cuts at:      0     576    1050    2298 
   Size:        576     474    1248

  Fragments arranged by size: 

                1248     576     474
 
NspI r_CATG'y

Cuts at:      0    1035    1350    1754    2028    2298 
   Size:       1035     315     404     274     270

  Fragments arranged by size: 

                1035     404     315     274     270
 
PciI A'CATG_T

Cuts at:      0    1346    2024    2298 
   Size:       1346     678     274

  Fragments arranged by size: 

                1346     678     274
 
PstI C_TGCA'G

Cuts at:      0    1505    2166    2298 
   Size:       1505     661     132

  Fragments arranged by size: 

                1505     661     132
 
PvuII CAG'CTG

Cuts at:      0     576    2298 
   Size:        576    1722
 
SexAI A'CCwGG_T

Cuts at:      0    2229    2298 
   Size:       2229      69
 
SfcI C'TryA_G

Cuts at:      0     465    1151    1501    2162    2298 
   Size:        465     686     350     661     136

  Fragments arranged by size: 

                 686     661     465     350     136
 
SmlI C'TyrA_G

Cuts at:      0     886    1075    2298 
   Size:        886     189    1223

  Fragments arranged by size: 

                1223     886     189
 
SspI AAT'ATT

Cuts at:      0     768    2298 
   Size:        768    1530
 
StyI C'CwwG_G

Cuts at:      0     527    1972    2098    2258    2298 
   Size:        527    1445     126     160      40

  Fragments arranged by size: 

                1445     527     160     126      40
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     438    2298 
   Size:        438    1860
 
TatI w'GTAC_w

Cuts at:      0    1355    2021    2298 
   Size:       1355     666     277

  Fragments arranged by size: 

                1355     666     277
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     890     922    1451    1483    1916    1948    2298 
   Size:        890      32     529      32     433      32     350

  Fragments arranged by size: 

                 890     529     433     350      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2136    2298 
   Size:       2136     162
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     276    1374    2070    2298 
   Size:        276    1098     696     228

  Fragments arranged by size: 

                1098     696     276     228
 
XmnI GAAnn'nnTTC

Cuts at:      0     450     956     986    1043    2298 
   Size:        450     506      30      57    1255

  Fragments arranged by size: 

                1255     506     450      57      30
 
ZraI GAC'GTC

Cuts at:      0    2046    2298 
   Size:       2046     252
 

 Enzymes that do cut:

    AatII     AcuI    AflII   AflIII     AlfI    AlwNI    ApaLI     ApoI
    BamHI     BanI    BanII     BbsI     BcgI     BclI     BglI    BglII
     BlpI Bme1580I     BmrI     BpmI    BsaBI    BsaHI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrDI    BsrGI
   BstAPI    BstBI    BstXI    BstYI     ClaI   DraIII     EaeI  Eco57MI
 EcoO109I     FalI     FspI    Hin4I   HincII  HindIII     MfeI     MmeI
     MscI   MspA1I     NspI     PciI     PstI    PvuII    SexAI     SfcI
     SmlI     SspI     StyI    TaqII     TatI     TstI  Tth111I     XcmI
     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI     AgeI     AhdI     AleI
     AloI     ApaI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
     BbeI    BbvCI    BciVI    BfrBI    BmgBI     BmtI     BplI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaWI    BsiEI    BsiWI    BsmBI    BspEI
    BspHI    BsrBI    BsrFI   BssHII    BssSI   BstEII  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI    CspCI     DraI     DrdI     EagI     EarI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI    FspAI    HaeII
     HpaI     KasI     KpnI     MluI     MslI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI     XbaI
     XhoI     XmaI
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