Restriction map for pF1KB9177
##################################
# NO CUT ENZYMES (   82 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AhdI        ApoI        AscI        AseI        
AsnI        Asp718I     AspEI       AvrII       BbeI        
BfrI        BglII       BlnI        BsaBI       BseAI       
BsiWI       Bsp1407I    BspDI       BspEI       BsrDI       
BsrGI       Bst1107I    BstBI       BstEII      BstPI       
BstZ17I     ClaI        CpoI        DraI        EagI        
Eam1105I    EclXI       Eco52I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FseI        HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MluI        MroI        MunI        NarI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PmeI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacII       SbfI        ScaI        
SfiI        SfoI        SfuI        SgrAI       SnaBI       
SpeI        Sse8387I    SspBI       SspI        SwaI        
TfiI        XbaI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AccI         1479  GTCGAC               GT^[AC][GT]AC
AgeI          983  ACCGGT               A^CCGGT
Aor51HI       110  AGCGCT               AGC^GCT
AspI          388  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         209  TGCGCA               TGC^GCA
BcgI          946  CGACTCCAGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1540  GGATAC               GTATCC(6/5)
BclI         2570  TGATCA               T^GATCA
BlpI         1560  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1560  GCTGAGC              GC^T[ACGT]AGC
BsiEI         198  CGACCG               CG[GA][CT]^CG
BsmI         2162  GCATTC               GAATGC(1/-1)
BspHI        1610  TCATGA               T^CATGA
BspLU11I     3356  ACATGT               A^CATGT
BssHII        241  GCGCGC               G^CGCGC
BssSI         295  CTCGTG               CACGAG(-5/-1)
CelII        1560  GCTGAGC              GC^T[ACGT]AGC
DraIII        400  CACACTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          123  GACTTGCGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      110  AGCGCT               AGC^GCT
FbaI         2570  TGATCA               T^GATCA
FspI          209  TGCGCA               TGC^GCA
HincII       1479  GTCGAC               GT[CT]^[GA]AC
HindII       1479  GTCGAC               GT[CT]^[GA]AC
HindIII       911  AAGCTT               A^AGCTT
NcoI         1469  CCATGG               C^CATGG
PaeR7I       2825  CTCGAG               C^TCGAG
PflFI         388  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI         983  ACCGGT               A^CCGGT
RcaI         1610  TCATGA               T^CATGA
SacI         3181  GAGCTC               GAGCT^C
SalI         1479  GTCGAC               G^TCGAC
SapI          474  GAAGAGC              GCTCTTC(1/4)
SexAI         257  ACCAGGT              A^CC[AT]GGT
SmlI         2825  CTCGAG               C^T[CT][GA]AG
Tsp509I      2678  AATT                 ^AATT
Tth111I       388  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         2825  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AocI         1524  CCTGAGG              CC^T[ACGT]AGG
             2092  CCTGAGG             
Asp700I      1128  GAACCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2916  GAAGCAGTTC          
BalI           83  TGGCCA               TGG^CCA
             3059  TGGCCA              
BamHI         484  GGATCC               G^GATCC
             1046  GGATCC              
BbrPI         437  CACGTG               CAC^GTG
             1906  CACGTG              
BbvCI        2227  GCTGAGG              CCTCAGC(-5/-2)
             3403  GCTGAGG             
BsaWI         983  ACCGGT               [AT]^CCGG[AT]
             1043  ACCGGA              
BsrBI         567  GAGCGG               CCGCTC(-3/-3)
             1774  GAGCGG              
Bsu36I       1524  CCTGAGG              CC^T[ACGT]AGG
             2092  CCTGAGG             
Eco81I       1524  CCTGAGG              CC^T[ACGT]AGG
             2092  CCTGAGG             
MluNI          83  TGGCCA               TGG^CCA
             3059  TGGCCA              
MscI           83  TGGCCA               TGG^CCA
             3059  TGGCCA              
MseI         1516  TTAA                 T^TAA
             2341  TTAA                
NaeI         1307  GCCGGC               GCC^GGC
             1565  GCCGGC              
NgoMIV       1307  GCCGGC               G^CCGGC
             1565  GCCGGC              
PflMI        1383  CCAGCTTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1469  CCATGGACTGG         
PmaCI         437  CACGTG               CAC^GTG
             1906  CACGTG              
PmlI          437  CACGTG               CAC^GTG
             1906  CACGTG              
SmaI          220  CCCGGG               CCC^GGG
             1714  CCCGGG              
Tru9I        1516  TTAA                 T^TAA
             2341  TTAA                
Van91I       1383  CCAGCTTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1469  CCATGGACTGG         
XmaCI         220  CCCGGG               C^CCGGG
             1714  CCCGGG              
XmaI          220  CCCGGG               C^CCGGG
             1714  CCCGGG              
XmnI         1128  GAACCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2916  GAAGCAGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  114 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AflIII      
AluI        Alw44I      AlwI        AlwNI       ApaI        
ApaLI       AspHI       AvaI        AvaII       BanI        
BanII       BbsI        BbvI        BcnI        BfaI        
BglI        BmyI        BpmI        BpuAI       BsaAI       
BsaHI       BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrFI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstUI       BstXI       BstYI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PpuMI       PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SphI        
StuI        StyI        TaqI        TseI        Tsp45I      
TspRI       TthHB8I     XcmI        XhoII       
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