Restriction map for pF1KB9177
 (Linear) (Six-base) MAPSORT of: KIBB9177.seq  Check: 2505  from: 1  to: 3414



KIBB9177.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:38  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     386    2034    2323    3414 
   Size:        386    1648     289    1091

  Fragments arranged by size: 

                1648    1091     386     289
 
AccI GT'mk_AC

Cuts at:      0    1480    3414 
   Size:       1480    1934
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2279    2608    3414 
   Size:       2279     329     806

  Fragments arranged by size: 

                2279     806     329
 
AfeI AGC'GCT

Cuts at:      0     112    3414 
   Size:        112    3302
 
AflIII A'CryG_T

Cuts at:      0     438    1905    3356    3414 
   Size:        438    1467    1451      58

  Fragments arranged by size: 

                1467    1451     438      58
 
AgeI A'CCGG_T

Cuts at:      0     983    3414 
   Size:        983    2431
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2236    3414 
   Size:       2236    1178
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     514     546    1249    1281    1532    1564    1777    1809 
   Size:        514      32     703      32     251      32     213      32

Cuts at:   1809    2959    2991    3414 
   Size:       1150      32     423

  Fragments arranged by size: 

                1150     703     514     423     251     213      32      32
                  32      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    2280    2366    2868    2907    3414 
   Size:       2280      86     502      39     507

  Fragments arranged by size: 

                2280     507     502      86      39
 
ApaI G_GGCC'C

Cuts at:      0     175    1888    2236    3414 
   Size:        175    1713     348    1178

  Fragments arranged by size: 

                1713    1178     348     175
 
ApaLI G'TGCA_C

Cuts at:      0     355     442    1024    3414 
   Size:        355      87     582    2390

  Fragments arranged by size: 

                2390     582     355      87
 
AvaI C'yCGr_G

Cuts at:      0     220    1714    2173    2406    2476    2825    3002    3414 
   Size:        220    1494     459     233      70     349     177     412

  Fragments arranged by size: 

                1494     459     412     349     233     220     177      70
            
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     186     219    3414 
   Size:        186      33    3195

  Fragments arranged by size: 

                3195     186      33
 
BamHI G'GATC_C

Cuts at:      0     484    1046    3414 
   Size:        484     562    2368

  Fragments arranged by size: 

                2368     562     484
 
BanI G'GyrC_C

Cuts at:      0      12     754    1236    1710    1837    3414 
   Size:         12     742     482     474     127    1577

  Fragments arranged by size: 

                1577     742     482     474     127      12
 
BanII G_rGCy'C

Cuts at:      0     160     175     375    1888    2174    2236    2302    2483 
   Size:        160      15     200    1513     286      62      66     181

Cuts at:   2483    3185    3414 
   Size:        702     229

  Fragments arranged by size: 

                1513     702     286     229     200     181     160      66
                  62      15
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     297    1222    3034    3414 
   Size:        297     925    1812     380

  Fragments arranged by size: 

                1812     925     380     297
 
BbvCI CC'TCA_GC

Cuts at:      0    2228    3404    3414 
   Size:       2228    1176      10

  Fragments arranged by size: 

                2228    1176      10
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     935     969    3414 
   Size:        935      34    2445

  Fragments arranged by size: 

                2445     935      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1534    3414 
   Size:       1534    1880
 
BclI T'GATC_A

Cuts at:      0    2570    3414 
   Size:       2570     844
 
BglI GCCn_nnn'nGGC

Cuts at:      0     608     743    3343    3414 
   Size:        608     135    2600      71

  Fragments arranged by size: 

                2600     608     135      71
 
BlpI GC'TnA_GC

Cuts at:      0    1561    3414 
   Size:       1561    1853
 
Bme1580I G_kGCm'C

Cuts at:      0      17     175     359     446     685     817     921    1028 
   Size:         17     158     184      87     239     132     104     107

Cuts at:   1028    1241    1337    1346    1715    1888    2236    3414 
   Size:        213      96       9     369     173     348    1178

  Fragments arranged by size: 

                1178     369     348     239     213     184     173     158
                 132     107     104      96      87      17       9
 
BmgBI CAC'GTC

Cuts at:      0     465    2082    3414 
   Size:        465    1617    1332

  Fragments arranged by size: 

                1617    1332     465
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1493    1916    2230    2488    3414 
   Size:       1493     423     314     258     926

  Fragments arranged by size: 

                1493     926     423     314     258
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1806    2643    3414 
   Size:       1806     837     771

  Fragments arranged by size: 

                1806     837     771
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     208     389     909     934    1099    1916    2202    3414 
   Size:        208     181     520      25     165     817     286    1212

  Fragments arranged by size: 

                1212     817     520     286     208     181     165      25
            
 
Bpu10I CC'TnA_GC

Cuts at:      0    1628    2228    2436    2485    2851    3404    3414 
   Size:       1628     600     208      49     366     553      10

  Fragments arranged by size: 

                1628     600     553     366     208      49      10
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1398    1410    2057    2410    3414 
   Size:       1398      12     647     353    1004

  Fragments arranged by size: 

                1398    1004     647     353      12
 
BsaAI yAC'GTr

Cuts at:      0     354     439    1908    3414 
   Size:        354      85    1469    1506

  Fragments arranged by size: 

                1506    1469     354      85
 
BsaHI Gr'CG_yC

Cuts at:      0     164     302    3039    3414 
   Size:        164     138    2737     375

  Fragments arranged by size: 

                2737     375     164     138
 
BsaWI w'CCGG_w

Cuts at:      0     983    1043    3414 
   Size:        983      60    2371

  Fragments arranged by size: 

                2371     983      60
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1346    1376    2959    2989    2999    3029    3414 
   Size:       1346      30    1583      30      10      30     385

  Fragments arranged by size: 

                1583    1346     385      30      30      30      10
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1595    2110    2428    3025    3414 
   Size:       1595     515     318     597     389

  Fragments arranged by size: 

                1595     597     515     389     318
 
BseYI C'CCAG_C

Cuts at:      0     107     325     649     656    2050    2814    3414 
   Size:        107     218     324       7    1394     764     600

  Fragments arranged by size: 

                1394     764     600     324     218     107       7
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     419     574    1136    1337    1429    1576    1857    1879 
   Size:        419     155     562     201      92     147     281      22

Cuts at:   1879    1886    2062    2078    3414 
   Size:          7     176      16    1336

  Fragments arranged by size: 

                1336     562     419     281     201     176     155     147
                  92      22      16       7
 
BsiEI CG_ry'CG

Cuts at:      0     201    3414 
   Size:        201    3213
 
BsiHKAI G_wGCw'C

Cuts at:      0     359     446    1028    1184    3185    3414 
   Size:        359      87     582     156    2001     229

  Fragments arranged by size: 

                2001     582     359     229     156      87
 
BsmI GAATG_Cn'

Cuts at:      0    2162    3414 
   Size:       2162    1252
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1087    1794    2373    3046    3414 
   Size:       1087     707     579     673     368

  Fragments arranged by size: 

                1087     707     673     579     368
 
Bsp1286I G_dGCh'C

Cuts at:      0      17     160     175     359     375     446     685     817 
   Size:         17     143      15     184      16      71     239     132

Cuts at:    817     921    1028    1184    1241    1337    1346    1715    1888 
   Size:        104     107     156      57      96       9     369     173

Cuts at:   1888    2174    2236    2302    2483    3185    3414 
   Size:        286      62      66     181     702     229

  Fragments arranged by size: 

                 702     369     286     239     229     184     181     173
                 156     143     132     107     104      96      71      66
                  62      57      17      16      15       9
 
BspHI T'CATG_A

Cuts at:      0    1610    3414 
   Size:       1610    1804
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      98     386    2034    2323    2780    3414 
   Size:         98     288    1648     289     457     634

  Fragments arranged by size: 

                1648     634     457     289     288      98
 
BsrBI CCG'CTC

Cuts at:      0     569    1776    3414 
   Size:        569    1207    1638

  Fragments arranged by size: 

                1638    1207     569
 
BsrFI r'CCGG_y

Cuts at:      0     983    1307    1451    1565    1679    3414 
   Size:        983     324     144     114     114    1735

  Fragments arranged by size: 

                1735     983     324     144     114     114
 
BssHII G'CGCG_C

Cuts at:      0     241    3414 
   Size:        241    3173
 
BssSI C'ACGA_G

Cuts at:      0     295    3414 
   Size:        295    3119
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1023    2366    2868    3414 
   Size:       1023    1343     502     546

  Fragments arranged by size: 

                1343    1023     546     502
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     591     606    1491    3414 
   Size:        591      15     885    1923

  Fragments arranged by size: 

                1923     885     591      15
 
BstYI r'GATC_y

Cuts at:      0     484     880    1046    1055    1976    3414 
   Size:        484     396     166       9     921    1438

  Fragments arranged by size: 

                1438     921     484     396     166       9
 
Bsu36I CC'TnA_GG

Cuts at:      0    1525    2093    3414 
   Size:       1525     568    1321

  Fragments arranged by size: 

                1525    1321     568
 
BtgI C'CryG_G

Cuts at:      0    1469    1645    2115    3414 
   Size:       1469     176     470    1299

  Fragments arranged by size: 

                1469    1299     470     176
 
BtsI GCAGTG_nn'

Cuts at:      0     817    1808    1869    3414 
   Size:        817     991      61    1545

  Fragments arranged by size: 

                1545     991     817      61
 
DraIII CAC_nnn'GTG

Cuts at:      0     405    3414 
   Size:        405    3009
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     129    3414 
   Size:        129    3285
 
EaeI y'GGCC_r

Cuts at:      0      83    1296    1567    2330    2461    2550    2888    3059 
   Size:         83    1213     271     763     131      89     338     171

Cuts at:   3059    3414 
   Size:        355

  Fragments arranged by size: 

                1213     763     355     338     271     171     131      89
                  83
 
EarI CTCTTCn'nnn_

Cuts at:      0      43     469    2149    2192    2255    3414 
   Size:         43     426    1680      43      63    1159

  Fragments arranged by size: 

                1680    1159     426      63      43      43
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1429    3414 
   Size:       1429    1985
 
EcoICRI GAG'CTC

Cuts at:      0    3183    3414 
   Size:       3183     231
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     208     389     909     934    1099    1916    2202    2279 
   Size:        208     181     520      25     165     817     286      77

Cuts at:   2279    2608    3414 
   Size:        329     806

  Fragments arranged by size: 

                 817     806     520     329     286     208     181     165
                  77      25
 
EcoO109I rG'GnC_Cy

Cuts at:      0     171     837    1589    1597    1959    2232    2265    2441 
   Size:        171     666     752       8     362     273      33     176

Cuts at:   2441    2526    2714    2799    2934    3414 
   Size:         85     188      85     135     480

  Fragments arranged by size: 

                 752     666     480     362     273     188     176     171
                 135      85      85      33       8
 
FspI TGC'GCA

Cuts at:      0     211    3414 
   Size:        211    3203
 
FspAI rTGC'GCAy

Cuts at:      0     211    3414 
   Size:        211    3203
 
HaeII r_GCGC'y

Cuts at:      0     114     617     657    1314    3414 
   Size:        114     503      40     657    2100

  Fragments arranged by size: 

                2100     657     503     114      40
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    3024    3056    3094    3126    3414 
   Size:       3024      32      38      32     288

  Fragments arranged by size: 

                3024     288      38      32      32
 
HincII GTy'rAC

Cuts at:      0    1481    3414 
   Size:       1481    1933
 
HindIII A'AGCT_T

Cuts at:      0     911    3414 
   Size:        911    2503
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1631    2940    3414 
   Size:       1631    1309     474

  Fragments arranged by size: 

                1631    1309     474
 
MscI TGG'CCA

Cuts at:      0      85    3061    3414 
   Size:         85    2976     353

  Fragments arranged by size: 

                2976     353      85
 
MslI CAynn'nnrTG

Cuts at:      0     352     701     716    1489    1609    2608    3414 
   Size:        352     349      15     773     120     999     806

  Fragments arranged by size: 

                 999     806     773     352     349     120      15
 
MspA1I CmG'CkG

Cuts at:      0     424     761    1120    1225    1401    1560    1851    1983 
   Size:        424     337     359     105     176     159     291     132

Cuts at:   1983    2257    2280    2865    3403    3414 
   Size:        274      23     585     538      11

  Fragments arranged by size: 

                 585     538     424     359     337     291     274     176
                 159     132     105      23      11
 
NaeI GCC'GGC

Cuts at:      0    1309    1567    3414 
   Size:       1309     258    1847

  Fragments arranged by size: 

                1847    1309     258
 
NcoI C'CATG_G

Cuts at:      0    1469    3414 
   Size:       1469    1945
 
NgoMIV G'CCGG_C

Cuts at:      0    1307    1565    3414 
   Size:       1307     258    1849

  Fragments arranged by size: 

                1849    1307     258
 
NspI r_CATG'y

Cuts at:      0    1666    2154    2901    3330    3360    3414 
   Size:       1666     488     747     429      30      54

  Fragments arranged by size: 

                1666     747     488     429      54      30
 
PasI CC'CwG_GG

Cuts at:      0     606    2208    3201    3414 
   Size:        606    1602     993     213

  Fragments arranged by size: 

                1602     993     606     213
 
PciI A'CATG_T

Cuts at:      0    3356    3414 
   Size:       3356      58
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1389    1475    3414 
   Size:       1389      86    1939

  Fragments arranged by size: 

                1939    1389      86
 
PmlI CAC'GTG

Cuts at:      0     439    1908    3414 
   Size:        439    1469    1506

  Fragments arranged by size: 

                1506    1469     439
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2905    2937    2959    2991    3414 
   Size:       2905      32      22      32     423

  Fragments arranged by size: 

                2905     423      32      32      22
 
PpuMI rG'GwC_Cy

Cuts at:      0    2441    2526    2799    2934    3414 
   Size:       2441      85     273     135     480

  Fragments arranged by size: 

                2441     480     273     135      85
 
PspOMI G'GGCC_C

Cuts at:      0     171    1884    2232    3414 
   Size:        171    1713     348    1182

  Fragments arranged by size: 

                1713    1182     348     171
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    2716    2748    3364    3396    3414 
   Size:       2716      32     616      32      18

  Fragments arranged by size: 

                2716     616      32      32      18
 
PstI C_TGCA'G

Cuts at:      0     542     778    1125    2225    2429    2474    3414 
   Size:        542     236     347    1100     204      45     940

  Fragments arranged by size: 

                1100     940     542     347     236     204      45
 
PvuII CAG'CTG

Cuts at:      0    1120    1225    1560    1851    1983    2257    2280    2865 
   Size:       1120     105     335     291     132     274      23     585

Cuts at:   2865    3403    3414 
   Size:        538      11

  Fragments arranged by size: 

                1120     585     538     335     291     274     132     105
                  23      11
 
SacI G_AGCT'C

Cuts at:      0    3185    3414 
   Size:       3185     229
 
SalI G'TCGA_C

Cuts at:      0    1479    3414 
   Size:       1479    1935
 
SapI GCTCTTCn'nnn_

Cuts at:      0     469    3414 
   Size:        469    2945
 
SexAI A'CCwGG_T

Cuts at:      0     257    3414 
   Size:        257    3157
 
SfcI C'TryA_G

Cuts at:      0     538     576     774    1121    2221    2425    2470    3078 
   Size:        538      38     198     347    1100     204      45     608

Cuts at:   3078    3313    3414 
   Size:        235     101

  Fragments arranged by size: 

                1100     608     538     347     235     204     198     101
                  45      38
 
SmaI CCC'GGG

Cuts at:      0     222    1716    3414 
   Size:        222    1494    1698

  Fragments arranged by size: 

                1698    1494     222
 
SmlI C'TyrA_G

Cuts at:      0    2825    3414 
   Size:       2825     589
 
SphI G_CATG'C

Cuts at:      0    2154    2901    3330    3414 
   Size:       2154     747     429      84

  Fragments arranged by size: 

                2154     747     429      84
 
SrfI GCCC'GGGC

Cuts at:      0     222    1716    3414 
   Size:        222    1494    1698

  Fragments arranged by size: 

                1698    1494     222
 
StuI AGG'CCT

Cuts at:      0     772    2091    3346    3414 
   Size:        772    1319    1255      68

  Fragments arranged by size: 

                1319    1255     772      68
 
StyI C'CwwG_G

Cuts at:      0     674    1193    1469    1706    3144    3414 
   Size:        674     519     276     237    1438     270

  Fragments arranged by size: 

                1438     674     519     276     270     237
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1001    1855    3414 
   Size:       1001     854    1559

  Fragments arranged by size: 

                1559    1001     854
 
TatI w'GTAC_w

Cuts at:      0     426    3414 
   Size:        426    2988
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1098    1130    1346    1378    1608    1640    2656    2688 
   Size:       1098      32     216      32     230      32    1016      32

Cuts at:   2688    3414 
   Size:        726

  Fragments arranged by size: 

                1098    1016     726     230     216      32      32      32
                  32
 
Tth111I GACn'n_nGTC

Cuts at:      0     391    3414 
   Size:        391    3023
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      39     964    1200    2031    2501    3414 
   Size:         39     925     236     831     470     913

  Fragments arranged by size: 

                 925     913     831     470     236      39
 
XhoI C'TCGA_G

Cuts at:      0    2825    3414 
   Size:       2825     589
 
XmaI C'CCGG_G

Cuts at:      0     220    1714    3414 
   Size:        220    1494    1700

  Fragments arranged by size: 

                1700    1494     220
 
XmnI GAAnn'nnTTC

Cuts at:      0    1132    2920    3414 
   Size:       1132    1788     494

  Fragments arranged by size: 

                1788    1132     494
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI   AflIII     AgeI     AlfI     AloI
    AlwNI     ApaI    ApaLI     AvaI     BaeI    BamHI     BanI    BanII
     BbsI    BbvCI     BcgI    BciVI     BclI     BglI     BlpI Bme1580I
    BmgBI     BmrI     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrFI   BssHII    BssSI
   BstAPI    BstXI    BstYI   Bsu36I     BtgI     BtsI   DraIII     DrdI
     EaeI     EarI     EciI  EcoICRI  Eco57MI EcoO109I     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     MmeI     MscI     MslI   MspA1I
     NaeI     NcoI   NgoMIV     NspI     PasI     PciI    PflMI     PmlI
     PpiI    PpuMI   PspOMI     PsrI     PstI    PvuII     SacI     SalI
     SapI    SexAI     SfcI     SmaI     SmlI     SphI     SrfI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XhoI     XmaI
     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII     AhdI     AleI     ApoI     AscI
     AseI    AsiSI    AvrII     BbeI    BfrBI    BglII     BmtI    BpuEI
    BsaBI    BsiWI    BspEI    BsrDI    BsrGI    BstBI   BstEII  BstZ17I
    BtgZI     ClaI    CspCI     DraI     EagI    EcoNI    EcoRI    EcoRV
     FalI     FseI     HpaI     KasI     KpnI     MfeI     MluI     NarI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PfoI     PmeI
    PshAI     PsiI    PspXI     PvuI    RsrII    SacII    SanDI     SbfI
     ScaI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SspI
     SwaI     XbaI     ZraI
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