Restriction map for pF1KB9148
##################################
# NO CUT ENZYMES (  120 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AgeI        AlwI        AocI        Aor51HI     
ApaI        AscI        AseI        AsnI        Asp718I     
AspI        AviII       AvrII       BamHI       BanI        
BbeI        BcgI        BclI        BcnI        BfrI        
BglI        BlnI        BsaBI       BsaHI       BseAI       
BsiEI       BsiWI       BsmBI       BsmFI       BspDI       
BspEI       BsrBI       BsrFI       BssHII      BssSI       
BstBI       BstEII      BstPI       BstXI       Bsu36I      
Cfr10I      ClaI        CpoI        DraI        DraIII      
EagI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoT14I     EcoT22I     
FbaI        FseI        FspI        HaeII       HgaI        
Hin1I       HindIII     HpaI        KasI        KpnI        
KspI        MamI        MluI        MroI        MslI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PflFI       PflMI       PinAI       
PmeI        PpuMI       PshBI       Psp1406I    PvuI        
RsrII       SacI        SacII       SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspI        StyI        SwaI        Tth111I     
Van91I      XbaI        XcmI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AccII        1611  CGCG                 CG^CG
AcsI         1450  GAATTT               [GA]^AATT[CT]
AflIII        580  ACATGT               A^C[GA][CT]GT
AhdI          153  GACCACCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        412  GTGCAC               G^TGCAC
AlwNI        1070  CAGAGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI         412  GTGCAC               G^TGCAC
ApoI         1450  GAATTT               [GA]^AATT[CT]
Asp700I       805  GAAAACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         153  GACCACCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI          125  CTCGAG               C^[CT]CG[GA]G
BanII        1051  GAGCCC               G[GA]GC[CT]^C
BbrPI         844  CACGTG               CAC^GTG
BbsI          742  GAAGAC               GAAGAC(2/6)
BbvCI        1120  CCTCAGC              CCTCAGC(-5/-2)
BciVI          30  GGATAC               GTATCC(6/5)
BfaI         1097  CTAG                 C^TAG
BpuAI         742  GAAGAC               GAAGAC(2/6)
BsaAI         844  CACGTG               [CT]AC^GT[GA]
BsaI          151  GAGACC               GGTCTC(1/5)
BsaWI        1277  ACCGGA               [AT]^CCGG[AT]
BsgI          841  CTGCAC               GTGCAG(16/14)
BsmI         1184  GAATGC               GAATGC(1/-1)
BsoBI         125  CTCGAG               C^[CT]CG[GA]G
Bsp1407I      398  TGTACA               T^GTACA
BspLU11I      580  ACATGT               A^CATGT
BspMI        1627  ACCTGC               ACCTGC(4/8)
BsrDI         555  CATTGC               GCAATG(2/0)
BsrGI         398  TGTACA               T^GTACA
Bst1107I      668  GTATAC               GTA^TAC
BstAPI        611  GCAAGAATTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI        1611  CGCG                 CG^CG
BstZ17I       668  GTATAC               GTA^TAC
DraII         787  AGGGCCT              [GA]G^G[ACGT]CC[CT]
DrdI          745  GACAATCAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          132  CCACGG               C^C[GA][CT]GG
Eam1105I      153  GACCACCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I      787  AGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoRV         817  GATATC               GAT^ATC
MaeI         1097  CTAG                 C^TAG
MaeIII       1003  GTCAC                ^GT[ACGT]AC
MfeI          499  CAATTG               C^AATTG
MunI          499  CAATTG               C^AATTG
MvnI         1611  CGCG                 CG^CG
PaeR7I        125  CTCGAG               C^TCGAG
PleI          128  GAGTC                GAGTC(4/5)
PmaCI         844  CACGTG               CAC^GTG
PmlI          844  CACGTG               CAC^GTG
PshAI         577  GACACATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          601  CTGCAG               CTGCA^G
SalI          754  GTCGAC               G^TCGAC
SspBI         398  TGTACA               T^GTACA
StuI          872  AGGCCT               AGG^CCT
Tsp45I       1003  GTCAC                ^GT[GC]AC
XhoI          125  CTCGAG               C^TCGAG
XmnI          805  GAAAACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AccI          668  GTATAC               GT^[AC][GT]AC
              754  GTCGAC              
AfaI          399  GTAC                 GT^AC
             1551  GTAC                
AvaII         942  GGACC                G^G[AT]CC
             1290  GGACC               
BalI          718  TGGCCA               TGG^CCA
              771  TGGCCA              
BglII         360  AGATCT               A^GATCT
             1256  AGATCT              
BlpI          165  GCTGAGC              GC^T[ACGT]AGC
              170  GCTTAGC             
BpmI          886  CTGGAG               CTGGAG(16/14)
             1330  CTGGAG              
Bpu1102I      165  GCTGAGC              GC^T[ACGT]AGC
              170  GCTTAGC             
BsaJI         132  CCACGG               C^C[ACGT][ACGT]GG
              945  CCGAGG              
BspHI         585  TCATGA               T^CATGA
              678  TCATGA              
BstYI         360  AGATCT               [GA]^GATC[CT]
             1256  AGATCT              
CelII         165  GCTGAGC              GC^T[ACGT]AGC
              170  GCTTAGC             
CfoI          852  GCGC                 GCG^C
             1623  GCGC                
DpnII         361  GATC                 ^GATC
             1257  GATC                
EaeI          718  TGGCCA               [CT]^GGCC[GA]
              771  TGGCCA              
FauI          338  CCCGC                CCCGC(4/6)
             1299  GCGGG               
HapII        1278  CCGG                 C^CGG
             1635  CCGG                
HhaI          852  GCGC                 GCG^C
             1623  GCGC                
HinP1I        852  GCGC                 G^CGC
             1623  GCGC                
HpaII        1278  CCGG                 C^CGG
             1635  CCGG                
MboI          361  GATC                 ^GATC
             1257  GATC                
MflI          360  AGATCT               [GA]^GATC[CT]
             1256  AGATCT              
MluNI         718  TGGCCA               TGG^CCA
              771  TGGCCA              
MscI          718  TGGCCA               TGG^CCA
              771  TGGCCA              
MspA1I        163  CAGCTG               C[AC]G^C[GT]G
              419  CAGCTG              
MspI         1278  CCGG                 C^CGG
             1635  CCGG                
NdeII         361  GATC                 ^GATC
             1257  GATC                
NlaIV          77  GGAACC               GG[ACGT]^[ACGT]CC
             1050  GGAGCC              
PvuII         163  CAGCTG               CAG^CTG
              419  CAGCTG              
RcaI          585  TCATGA               T^CATGA
              678  TCATGA              
RsaI          399  GTAC                 GT^AC
             1551  GTAC                
Sau3AI        361  GATC                 ^GATC
             1257  GATC                
SmlI          125  CTCGAG               C^T[CT][GA]AG
             1007  CTCAAG              
XhoII         360  AGATCT               [GA]^GATC[CT]
             1256  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   48 enzymes) #
#########################################

AciI        AluI        AspHI       BbvI        BmyI        
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
Cac8I       Cfr13I      DdeI        EarI        EcoRII      
Fnu4HI      FokI        HaeIII      HincII      HindII      
HinfI       HphI        ItaI        Ksp632I     MaeII       
MboII       MnlI        MseI        MvaI        MwoI        
NlaIII      NspI        Sau96I      ScrFI       SfaNI       
SfcI        TaqI        TfiI        Tru9I       TseI        
Tsp509I     TspRI       TthHB8I     
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