Restriction map for pF1KB9148
 (Linear) (Six-base) MAPSORT of: KIBB9148.seq  Check: 3060  from: 1  to: 1644



KIBB9148.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:38  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1636    1644 
   Size:       1636       8
 
AccI GT'mk_AC

Cuts at:      0     669     755    1644 
   Size:        669      86     889

  Fragments arranged by size: 

                 889     669      86
 
AflIII A'CryG_T

Cuts at:      0     580    1644 
   Size:        580    1064
 
AhdI GACnn_n'nnGTC

Cuts at:      0     158    1644 
   Size:        158    1486
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1075    1644 
   Size:       1075     569
 
ApaLI G'TGCA_C

Cuts at:      0     412    1644 
   Size:        412    1232
 
ApoI r'AATT_y

Cuts at:      0    1450    1644 
   Size:       1450     194
 
AvaI C'yCGr_G

Cuts at:      0     125    1644 
   Size:        125    1519
 
BanII G_rGCy'C

Cuts at:      0    1055    1644 
   Size:       1055     589
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     749    1644 
   Size:        749     895
 
BbvCI CC'TCA_GC

Cuts at:      0    1121    1644 
   Size:       1121     523
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      24    1644 
   Size:         24    1620
 
BglII A'GATC_T

Cuts at:      0     360    1256    1644 
   Size:        360     896     388

  Fragments arranged by size: 

                 896     388     360
 
BlpI GC'TnA_GC

Cuts at:      0     166     171    1644 
   Size:        166       5    1473

  Fragments arranged by size: 

                1473     166       5
 
Bme1580I G_kGCm'C

Cuts at:      0     316     416    1644 
   Size:        316     100    1228

  Fragments arranged by size: 

                1228     316     100
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     907    1351    1644 
   Size:        907     444     293

  Fragments arranged by size: 

                 907     444     293
 
Bpu10I CC'TnA_GC

Cuts at:      0    1121    1644 
   Size:       1121     523
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     992    1644 
   Size:        992     652
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     145    1644 
   Size:        145    1499
 
BsaAI yAC'GTr

Cuts at:      0     846    1644 
   Size:        846     798
 
BsaWI w'CCGG_w

Cuts at:      0    1277    1644 
   Size:       1277     367
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1534    1564    1644 
   Size:       1534      30      80

  Fragments arranged by size: 

                1534      80      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     966    1552    1555    1644 
   Size:        966     586       3      89

  Fragments arranged by size: 

                 966     586      89       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     826    1644 
   Size:        826     818
 
BsiHKAI G_wGCw'C

Cuts at:      0     416    1201    1604    1619    1644 
   Size:        416     785     403      15      25

  Fragments arranged by size: 

                 785     416     403      25      15
 
BsmI GAATG_Cn'

Cuts at:      0    1190    1644 
   Size:       1190     454
 
Bsp1286I G_dGCh'C

Cuts at:      0     316     416    1055    1201    1604    1619    1644 
   Size:        316     100     639     146     403      15      25

  Fragments arranged by size: 

                 639     403     316     146     100      25      15
 
BspHI T'CATG_A

Cuts at:      0     585     678    1644 
   Size:        585      93     966

  Fragments arranged by size: 

                 966     585      93
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1636    1644 
   Size:       1636       8
 
BsrDI GCAATG_nn'

Cuts at:      0     554    1644 
   Size:        554    1090
 
BsrGI T'GTAC_A

Cuts at:      0     398    1644 
   Size:        398    1246
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     617    1644 
   Size:        617    1027
 
BstYI r'GATC_y

Cuts at:      0     360    1256    1644 
   Size:        360     896     388

  Fragments arranged by size: 

                 896     388     360
 
BstZ17I GTA'TAC

Cuts at:      0     670    1644 
   Size:        670     974
 
BtgI C'CryG_G

Cuts at:      0     132    1644 
   Size:        132    1512
 
BtsI GCAGTG_nn'

Cuts at:      0    1525    1554    1644 
   Size:       1525      29      90

  Fragments arranged by size: 

                1525      90      29
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     751    1644 
   Size:        751     893
 
EaeI y'GGCC_r

Cuts at:      0     718     771    1644 
   Size:        718      53     873

  Fragments arranged by size: 

                 873     718      53
 
EarI CTCTTCn'nnn_

Cuts at:      0     473     950    1179    1644 
   Size:        473     477     229     465

  Fragments arranged by size: 

                 477     473     465     229
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      43    1644 
   Size:         43    1601
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     907    1351    1644 
   Size:        907     444     293

  Fragments arranged by size: 

                 907     444     293
 
EcoO109I rG'GnC_Cy

Cuts at:      0     788    1644 
   Size:        788     856
 
EcoRV GAT'ATC

Cuts at:      0     819    1644 
   Size:        819     825
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     205     636    1190    1644 
   Size:        205     431     554     454

  Fragments arranged by size: 

                 554     454     431     205
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     144     176     317     349     949     981    1644 
   Size:        144      32     141      32     600      32     663

  Fragments arranged by size: 

                 663     600     144     141      32      32      32
 
HincII GTy'rAC

Cuts at:      0     756     828    1148    1644 
   Size:        756      72     320     496

  Fragments arranged by size: 

                 756     496     320      72
 
MfeI C'AATT_G

Cuts at:      0     499    1644 
   Size:        499    1145
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     844    1268    1644 
   Size:        844     424     376

  Fragments arranged by size: 

                 844     424     376
 
MscI TGG'CCA

Cuts at:      0     720     773    1644 
   Size:        720      53     871

  Fragments arranged by size: 

                 871     720      53
 
MspA1I CmG'CkG

Cuts at:      0     165     421    1644 
   Size:        165     256    1223

  Fragments arranged by size: 

                1223     256     165
 
NspI r_CATG'y

Cuts at:      0     584    1236    1398    1644 
   Size:        584     652     162     246

  Fragments arranged by size: 

                 652     584     246     162
 
PciI A'CATG_T

Cuts at:      0     580    1644 
   Size:        580    1064
 
PmlI CAC'GTG

Cuts at:      0     846    1644 
   Size:        846     798
 
PshAI GACnn'nnGTC

Cuts at:      0     581    1644 
   Size:        581    1063
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1558    1590    1644 
   Size:       1558      32      54

  Fragments arranged by size: 

                1558      54      32
 
PstI C_TGCA'G

Cuts at:      0     605    1644 
   Size:        605    1039
 
PvuII CAG'CTG

Cuts at:      0     165     421    1644 
   Size:        165     256    1223

  Fragments arranged by size: 

                1223     256     165
 
SalI G'TCGA_C

Cuts at:      0     754    1644 
   Size:        754     890
 
SfcI C'TryA_G

Cuts at:      0     356     601    1385    1644 
   Size:        356     245     784     259

  Fragments arranged by size: 

                 784     356     259     245
 
SmlI C'TyrA_G

Cuts at:      0     125    1007    1644 
   Size:        125     882     637

  Fragments arranged by size: 

                 882     637     125
 
StuI AGG'CCT

Cuts at:      0     874    1644 
   Size:        874     770
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     959    1216    1644 
   Size:        959     257     428

  Fragments arranged by size: 

                 959     428     257
 
TatI w'GTAC_w

Cuts at:      0     398    1644 
   Size:        398    1246
 
XhoI C'TCGA_G

Cuts at:      0     125    1644 
   Size:        125    1519
 
XmnI GAAnn'nnTTC

Cuts at:      0     809    1644 
   Size:        809     835
 

 Enzymes that do cut:

     AarI     AccI   AflIII     AhdI    AlwNI    ApaLI     ApoI     AvaI
    BanII     BbsI    BbvCI    BciVI    BglII     BlpI Bme1580I     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaWI    BsaXI    BseRI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrDI    BsrGI   BstAPI
    BstYI  BstZ17I     BtgI     BtsI     DrdI     EaeI     EarI     EciI
  Eco57MI EcoO109I    EcoRV     FalI    Hin4I   HincII     MfeI     MmeI
     MscI   MspA1I     NspI     PciI     PmlI    PshAI     PsrI     PstI
    PvuII     SalI     SfcI     SmlI     StuI    TaqII     TatI     XhoI
     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI     AleI
     AlfI     AloI     ApaI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BanI     BbeI     BcgI     BclI    BfrBI     BglI    BmgBI
     BmrI     BmtI     BplI    BsaBI    BsaHI    BseYI    BsiEI    BsiWI
    BsmBI    BspEI    BsrBI    BsrFI   BssHII    BssSI    BstBI   BstEII
    BstXI   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     EagI
  EcoICRI    EcoNI    EcoRI     FseI     FspI    FspAI    HaeII  HindIII
     HpaI     KasI     KpnI     MluI     MslI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
    PflMI     PfoI     PmeI     PpiI    PpuMI     PsiI   PspOMI    PspXI
     PvuI    RsrII     SacI    SacII    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StyI     SwaI     TstI  Tth111I     XbaI
     XcmI     XmaI     ZraI
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