Restriction map for pF1KB7019
 (Linear) (Six-base) MAPSORT of: KIBB7019.seq  Check: 6521  from: 1  to: 2253



KIBB7019.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:38  ..

AccI GT'mk_AC

Cuts at:      0    1285    2253 
   Size:       1285     968
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     117    2253 
   Size:        117    2136
 
AflIII A'CryG_T

Cuts at:      0     678    2253 
   Size:        678    1575
 
AhdI GACnn_n'nnGTC

Cuts at:      0    2019    2253 
   Size:       2019     234
 
AleI CACnn'nnGTG

Cuts at:      0     332    2253 
   Size:        332    1921
 
AlwNI CAG_nnn'CTG

Cuts at:      0     586    2253 
   Size:        586    1667
 
ApaI G_GGCC'C

Cuts at:      0    1076    2253 
   Size:       1076    1177
 
ApoI r'AATT_y

Cuts at:      0    1106    1469    2253 
   Size:       1106     363     784

  Fragments arranged by size: 

                1106     784     363
 
AseI AT'TA_AT

Cuts at:      0     172    2253 
   Size:        172    2081
 
AvaI C'yCGr_G

Cuts at:      0     945    2253 
   Size:        945    1308
 
BanII G_rGCy'C

Cuts at:      0    1076    1415    2253 
   Size:       1076     339     838

  Fragments arranged by size: 

                1076     838     339
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     890    1659    2253 
   Size:        890     769     594

  Fragments arranged by size: 

                 890     769     594
 
BclI T'GATC_A

Cuts at:      0     533     630    2253 
   Size:        533      97    1623

  Fragments arranged by size: 

                1623     533      97
 
Bme1580I G_kGCm'C

Cuts at:      0    1076    1779    2253 
   Size:       1076     703     474

  Fragments arranged by size: 

                1076     703     474
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1643    2253 
   Size:       1643     610
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     567    2253 
   Size:        567    1686
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1441    1789    2253 
   Size:       1441     348     464

  Fragments arranged by size: 

                1441     464     348
 
Bpu10I CC'TnA_GC

Cuts at:      0     713    2253 
   Size:        713    1540
 
BsaBI GATnn'nnATC

Cuts at:      0     182    2253 
   Size:        182    2071
 
BsaWI w'CCGG_w

Cuts at:      0       8     191    2253 
   Size:          8     183    2062

  Fragments arranged by size: 

                2062     183       8
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1865    1895    2253 
   Size:       1865      30     358

  Fragments arranged by size: 

                1865     358      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     450     556    1154    1438    1659    2253 
   Size:        450     106     598     284     221     594

  Fragments arranged by size: 

                 598     594     450     284     221     106
 
BseYI C'CCAG_C

Cuts at:      0      68    2253 
   Size:         68    2185
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1516    1756    2253 
   Size:       1516     240     497

  Fragments arranged by size: 

                1516     497     240
 
BsiHKAI G_wGCw'C

Cuts at:      0     763     871    1415    2253 
   Size:        763     108     544     838

  Fragments arranged by size: 

                 838     763     544     108
 
BsmI GAATG_Cn'

Cuts at:      0       4     304     621    2086    2253 
   Size:          4     300     317    1465     167

  Fragments arranged by size: 

                1465     317     300     167       4
 
Bsp1286I G_dGCh'C

Cuts at:      0     763     871    1076    1415    1779    2253 
   Size:        763     108     205     339     364     474

  Fragments arranged by size: 

                 763     474     364     339     205     108
 
BspEI T'CCGG_A

Cuts at:      0       8    2253 
   Size:          8    2245
 
BspHI T'CATG_A

Cuts at:      0     458    2253 
   Size:        458    1795
 
BsrBI CCG'CTC

Cuts at:      0    1892    2253 
   Size:       1892     361
 
BsrDI GCAATG_nn'

Cuts at:      0    1928    2253 
   Size:       1928     325
 
BsrGI T'GTAC_A

Cuts at:      0    1220    2253 
   Size:       1220    1033
 
BstBI TT'CG_AA

Cuts at:      0     597    2253 
   Size:        597    1656
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1549    1767    2253 
   Size:       1549     218     486

  Fragments arranged by size: 

                1549     486     218
 
BstYI r'GATC_y

Cuts at:      0      61     318    2253 
   Size:         61     257    1935

  Fragments arranged by size: 

                1935     257      61
 
BstZ17I GTA'TAC

Cuts at:      0    1286    2253 
   Size:       1286     967
 
BtgI C'CryG_G

Cuts at:      0    2026    2253 
   Size:       2026     227
 
BtsI GCAGTG_nn'

Cuts at:      0    1923    2253 
   Size:       1923     330
 
EaeI y'GGCC_r

Cuts at:      0     271    1605    2253 
   Size:        271    1334     648

  Fragments arranged by size: 

                1334     648     271
 
EcoICRI GAG'CTC

Cuts at:      0    1413    2253 
   Size:       1413     840
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     117    1441    1789    2253 
   Size:        117    1324     348     464

  Fragments arranged by size: 

                1324     464     348     117
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1072    1584    2199    2253 
   Size:       1072     512     615      54

  Fragments arranged by size: 

                1072     615     512      54
 
EcoRI G'AATT_C

Cuts at:      0    1106    2253 
   Size:       1106    1147
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     738    1989    2253 
   Size:        738    1251     264

  Fragments arranged by size: 

                1251     738     264
 
HaeII r_GCGC'y

Cuts at:      0     401    2253 
   Size:        401    1852
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     351     383    1080    1112    1319    1351    1871    1903 
   Size:        351      32     697      32     207      32     520      32

Cuts at:   1903    2005    2037    2253 
   Size:        102      32     216

  Fragments arranged by size: 

                 697     520     351     216     207     102      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0     831    2253 
   Size:        831    1422
 
HindIII A'AGCT_T

Cuts at:      0     254    2253 
   Size:        254    1999
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0       9    2194    2253 
   Size:          9    2185      59

  Fragments arranged by size: 

                2185      59       9
 
MscI TGG'CCA

Cuts at:      0     273    1607    2253 
   Size:        273    1334     646

  Fragments arranged by size: 

                1334     646     273
 
MslI CAynn'nnrTG

Cuts at:      0     332     380     905    1547    2253 
   Size:        332      48     525     642     706

  Fragments arranged by size: 

                 706     642     525     332      48
 
MspA1I CmG'CkG

Cuts at:      0    1086    1538    1726    2253 
   Size:       1086     452     188     527

  Fragments arranged by size: 

                1086     527     452     188
 
NspI r_CATG'y

Cuts at:      0     682    2253 
   Size:        682    1571
 
PciI A'CATG_T

Cuts at:      0     678    2253 
   Size:        678    1575
 
PfoI T'CCnGG_A

Cuts at:      0    1134    2253 
   Size:       1134    1119
 
PpuMI rG'GwC_Cy

Cuts at:      0    1584    2253 
   Size:       1584     669
 
PspOMI G'GGCC_C

Cuts at:      0    1072    2253 
   Size:       1072    1181
 
PvuII CAG'CTG

Cuts at:      0    1086    1726    2253 
   Size:       1086     640     527

  Fragments arranged by size: 

                1086     640     527
 
SacI G_AGCT'C

Cuts at:      0    1415    2253 
   Size:       1415     838
 
SanDI GG'GwC_CC

Cuts at:      0    1584    2253 
   Size:       1584     669
 
SfcI C'TryA_G

Cuts at:      0     159    1934    2253 
   Size:        159    1775     319

  Fragments arranged by size: 

                1775     319     159
 
SpeI A'CTAG_T

Cuts at:      0     957    2253 
   Size:        957    1296
 
SspI AAT'ATT

Cuts at:      0     234    2253 
   Size:        234    2019
 
StuI AGG'CCT

Cuts at:      0    1251    2253 
   Size:       1251    1002
 
StyI C'CwwG_G

Cuts at:      0    1169    1911    2253 
   Size:       1169     742     342

  Fragments arranged by size: 

                1169     742     342
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1167    2253 
   Size:       1167    1086
 
TatI w'GTAC_w

Cuts at:      0     415     719    1220    2253 
   Size:        415     304     501    1033

  Fragments arranged by size: 

                1033     501     415     304
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     208     240     668     700     992    1024    1865    1897 
   Size:        208      32     428      32     292      32     841      32

Cuts at:   1897    2253 
   Size:        356

  Fragments arranged by size: 

                 841     428     356     292     208      32      32      32
                  32
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AhdI     AleI    AlwNI     ApaI     ApoI
     AseI     AvaI    BanII     BbsI     BclI Bme1580I     BmrI     BplI
     BpmI   Bpu10I    BsaBI    BsaWI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BsrBI    BsrDI    BsrGI
    BstBI    BstXI    BstYI  BstZ17I     BtgI     BtsI     EaeI  EcoICRI
  Eco57MI EcoO109I    EcoRI     FalI    HaeII    Hin4I   HincII  HindIII
     MmeI     MscI     MslI   MspA1I     NspI     PciI     PfoI    PpuMI
   PspOMI    PvuII     SacI    SanDI     SfcI     SpeI     SspI     StuI
     StyI    TaqII     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AlfI
     AloI    ApaLI     AscI    AsiSI    AvrII     BaeI    BamHI     BanI
     BbeI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmtI    BpuEI     BsaI    BsaAI    BsaHI    BsiEI    BsiWI
    BsmBI    BspMI    BsrFI   BssHII    BssSI   BstAPI   BstEII   Bsu36I
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI
     EciI    EcoNI    EcoRV     FseI     FspI    FspAI     HpaI     KasI
     KpnI     MfeI     MluI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI     PmeI
     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI
    RsrII    SacII     SalI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SphI     SrfI
     SwaI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com