(Circular) (Six-base) MAPSORT of: pF1KB5664.seq Check: 5534 from: 1 to: 4247 pF1KB5664 4247 bp With 182 enzymes: SgfI * June 20, 2008 10:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 493 493 Size: 4247 AccI GT'mk_AC Cuts at: 1285 1285 Size: 4247 Acc65I G'GTAC_C Cuts at: 1263 1263 Size: 4247 AclI AA'CG_TT Cuts at: 1241 4087 1241 Size: 2846 1401 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 223 1204 2072 2504 2857 3519 223 Size: 981 868 432 353 662 951 Fragments arranged by size: 981 951 868 662 432 353 AflIII A'CryG_T Cuts at: 1599 3399 3680 1599 Size: 1800 281 2166 Fragments arranged by size: 2166 1800 281 AleI CACnn'nnGTG Cuts at: 235 235 Size: 4247 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1036 1036 Size: 4247 AlwNI CAG_nnn'CTG Cuts at: 1552 2990 1552 Size: 1438 2809 ApaI G_GGCC'C Cuts at: 1077 1077 Size: 4247 ApaLI G'TGCA_C Cuts at: 3085 3085 Size: 4247 ApoI r'AATT_y Cuts at: 313 1251 3600 3856 313 Size: 938 2349 256 704 Fragments arranged by size: 2349 938 704 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4247 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4247 AvaI C'yCGr_G Cuts at: 1267 1267 Size: 4247 BamHI G'GATC_C Cuts at: 1272 1272 Size: 4247 BanI G'GyrC_C Cuts at: 560 613 1263 1677 1925 1960 560 Size: 53 650 414 248 35 2847 Fragments arranged by size: 2847 650 414 248 53 35 BanII G_rGCy'C Cuts at: 1077 1261 2291 1077 Size: 184 1030 3033 Fragments arranged by size: 3033 1030 184 BbeI G_GCGC'C Cuts at: 617 1681 1929 617 Size: 1064 248 2935 Fragments arranged by size: 2935 1064 248 BbsI GAAGACnn'nnnn_ Cuts at: 418 561 1172 418 Size: 143 611 3493 Fragments arranged by size: 3493 611 143 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 246 280 444 478 246 Size: 34 164 34 4015 Fragments arranged by size: 4015 164 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2145 3201 4247 2145 Size: 1056 1046 2145 Fragments arranged by size: 2145 1056 1046 BclI T'GATC_A Cuts at: 527 1767 527 Size: 1240 3007 BglI GCCn_nnn'nGGC Cuts at: 475 475 Size: 4247 BglII A'GATC_T Cuts at: 1762 3799 1762 Size: 2037 2210 BlpI GC'TnA_GC Cuts at: 1359 1359 Size: 4247 Bme1580I G_kGCm'C Cuts at: 931 1077 1872 1965 3089 931 Size: 146 795 93 1124 2089 Fragments arranged by size: 2089 1124 795 146 93 BmrI ACTGGGnnnn_n' Cuts at: 1459 1540 1875 4019 1459 Size: 81 335 2144 1687 Fragments arranged by size: 2144 1687 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2614 2614 Size: 4247 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 118 118 Size: 4247 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1423 2302 3016 3314 1423 Size: 879 714 298 2356 Fragments arranged by size: 2356 879 714 298 BsaI GGTCTCn'nnnn_ Cuts at: 148 3951 148 Size: 3803 444 BsaAI yAC'GTr Cuts at: 180 1600 180 Size: 1420 2827 BsaBI GATnn'nnATC Cuts at: 6 295 1761 3798 6 Size: 289 1466 2037 455 Fragments arranged by size: 2037 1466 455 289 BsaHI Gr'CG_yC Cuts at: 478 548 614 1678 1926 2628 4124 478 Size: 70 66 1064 248 702 1496 601 Fragments arranged by size: 1496 1064 702 601 248 70 66 BsaWI w'CCGG_w Cuts at: 1436 1957 2635 3046 3193 3467 1436 Size: 521 678 411 147 274 2216 Fragments arranged by size: 2216 678 521 411 274 147 BseYI C'CCAG_C Cuts at: 609 1672 2430 3095 3503 609 Size: 1063 758 665 408 1353 Fragments arranged by size: 1353 1063 758 665 408 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 628 940 628 Size: 312 3935 BsiEI CG_ry'CG Cuts at: 104 1789 1835 3065 104 Size: 1685 46 1230 1286 Fragments arranged by size: 1685 1286 1230 46 BsiHKAI G_wGCw'C Cuts at: 242 974 1261 2039 2229 3089 242 Size: 732 287 778 190 860 1400 Fragments arranged by size: 1400 860 778 732 287 190 BsmBI CGTCTCn'nnnn_ Cuts at: 136 136 Size: 4247 Bsp1286I G_dGCh'C Cuts at: 242 931 974 1077 1261 1872 1965 2039 2229 Size: 689 43 103 184 611 93 74 190 Cuts at: 2229 2291 3089 242 Size: 62 798 1400 Fragments arranged by size: 1400 798 689 611 190 184 103 93 74 62 43 BspHI T'CATG_A Cuts at: 2679 2679 Size: 4247 BspMI ACCTGCnnnn'nnnn_ Cuts at: 371 493 1178 1297 1511 1813 2194 371 Size: 122 685 119 214 302 381 2424 Fragments arranged by size: 2424 685 381 302 214 122 119 BsrBI CCG'CTC Cuts at: 50 513 816 2539 2593 4077 4242 50 Size: 463 303 1723 54 1484 165 55 Fragments arranged by size: 1723 1484 463 303 165 55 54 BsrDI GCAATG_nn' Cuts at: 596 695 2159 596 Size: 99 1464 2684 Fragments arranged by size: 2684 1464 99 BsrFI r'CCGG_y Cuts at: 138 370 2245 2426 138 Size: 232 1875 181 1959 Fragments arranged by size: 1959 1875 232 181 BssSI C'ACGA_G Cuts at: 2518 3226 2518 Size: 708 3539 BstAPI GCAn_nnn'nTGC Cuts at: 1624 1624 Size: 4247 BstBI TT'CG_AA Cuts at: 2608 2608 Size: 4247 BstEII G'GTnAC_C Cuts at: 732 732 Size: 4247 BstYI r'GATC_y Cuts at: 1 1229 1272 1739 1762 2097 2343 2649 2661 Size: 1228 43 467 23 335 246 306 12 Cuts at: 2661 2747 3799 1 Size: 86 1052 449 Fragments arranged by size: 1228 1052 467 449 335 306 246 86 43 23 12 Bsu36I CC'TnA_GG Cuts at: 445 445 Size: 4247 BtgI C'CryG_G Cuts at: 107 431 821 839 107 Size: 324 390 18 3515 Fragments arranged by size: 3515 390 324 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 360 486 849 2197 2378 2541 360 Size: 126 363 1348 181 163 2066 Fragments arranged by size: 2066 1348 363 181 163 126 BtsI GCAGTG_nn' Cuts at: 826 1490 826 Size: 664 3583 DraI TTT'AAA Cuts at: 1246 1246 Size: 4247 DrdI GACnn_nn'nnGTC Cuts at: 1953 3297 1953 Size: 1344 2903 EaeI y'GGCC_r Cuts at: 368 497 722 794 1007 1832 2006 2397 2424 Size: 129 225 72 213 825 174 391 27 Cuts at: 2424 3769 368 Size: 1345 846 Fragments arranged by size: 1345 846 825 391 225 213 174 129 72 27 EagI C'GGCC_G Cuts at: 1832 1832 Size: 4247 EarI CTCTTCn'nnn_ Cuts at: 247 889 898 943 2270 2480 247 Size: 642 9 45 1327 210 2014 Fragments arranged by size: 2014 1327 642 210 45 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 969 2455 3197 3343 4057 969 Size: 1486 742 146 714 1159 Fragments arranged by size: 1486 1159 742 714 146 EcoICRI GAG'CTC Cuts at: 1259 1259 Size: 4247 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 118 223 1204 2072 2504 2857 3519 118 Size: 105 981 868 432 353 662 846 Fragments arranged by size: 981 868 846 662 432 353 105 EcoNI CCTnn'n_nnAGG Cuts at: 448 940 448 Size: 492 3755 EcoO109I rG'GnC_Cy Cuts at: 1073 1386 1073 Size: 313 3934 EcoRI G'AATT_C Cuts at: 1251 1251 Size: 4247 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2266 2266 Size: 4247 FspI TGC'GCA Cuts at: 2028 2028 Size: 4247 HaeII r_GCGC'y Cuts at: 617 1681 1929 3159 3930 617 Size: 1064 248 1230 771 934 Fragments arranged by size: 1230 1064 934 771 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 2089 2119 2121 2151 2089 Size: 30 2 30 4185 Fragments arranged by size: 4185 30 30 2 HincII GTy'rAC Cuts at: 390 1286 390 Size: 896 3351 KasI G'GCGC_C Cuts at: 613 1677 1925 613 Size: 1064 248 2935 Fragments arranged by size: 2935 1064 248 KpnI G_GTAC'C Cuts at: 1267 1267 Size: 4247 MluI A'CGCG_T Cuts at: 3680 3680 Size: 4247 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 3006 3190 3006 Size: 184 4063 MscI TGG'CCA Cuts at: 499 796 1009 2008 499 Size: 297 213 999 2738 Fragments arranged by size: 2738 999 297 213 MslI CAynn'nnrTG Cuts at: 235 397 730 904 2236 2363 235 Size: 162 333 174 1332 127 2119 Fragments arranged by size: 2119 1332 333 174 162 127 MspA1I CmG'CkG Cuts at: 1358 1672 2032 2816 3061 1358 Size: 314 360 784 245 2544 Fragments arranged by size: 2544 784 360 314 245 NaeI GCC'GGC Cuts at: 372 2428 372 Size: 2056 2191 NarI GG'CG_CC Cuts at: 614 1678 1926 614 Size: 1064 248 2935 Fragments arranged by size: 2935 1064 248 NcoI C'CATG_G Cuts at: 107 821 107 Size: 714 3533 NgoMIV G'CCGG_C Cuts at: 370 2426 370 Size: 2056 2191 NspI r_CATG'y Cuts at: 330 1300 1800 3403 330 Size: 970 500 1603 1174 Fragments arranged by size: 1603 1174 970 500 PciI A'CATG_T Cuts at: 3399 3399 Size: 4247 PflMI CCAn_nnn'nTGG Cuts at: 180 437 180 Size: 257 3990 PmeI GTTT'AAAC Cuts at: 1246 1246 Size: 4247 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2693 2725 2693 Size: 32 4215 PspOMI G'GGCC_C Cuts at: 1073 1073 Size: 4247 PstI C_TGCA'G Cuts at: 259 490 916 1294 1979 259 Size: 231 426 378 685 2527 Fragments arranged by size: 2527 685 426 378 231 PvuI CG_AT'CG Cuts at: 104 104 Size: 4247 PvuII CAG'CTG Cuts at: 1672 2032 1672 Size: 360 3887 RsrII CG'GwC_CG Cuts at: 836 2442 836 Size: 1606 2641 SacI G_AGCT'C Cuts at: 1261 1261 Size: 4247 SalI G'TCGA_C Cuts at: 1284 1284 Size: 4247 SapI GCTCTTCn'nnn_ Cuts at: 247 2270 2480 247 Size: 2023 210 2014 Fragments arranged by size: 2023 2014 210 SbfI CC_TGCA'GG Cuts at: 916 1294 916 Size: 378 3869 ScaI AGT'ACT Cuts at: 3764 3764 Size: 4247 SfcI C'TryA_G Cuts at: 33 255 486 912 1290 1975 2943 3134 33 Size: 222 231 426 378 685 968 191 1146 Fragments arranged by size: 1146 968 685 426 378 231 222 191 SfoI GGC'GCC Cuts at: 615 1679 1927 615 Size: 1064 248 2935 Fragments arranged by size: 2935 1064 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4247 SmaI CCC'GGG Cuts at: 1269 1269 Size: 4247 SmlI C'TyrA_G Cuts at: 1402 2317 3031 3293 1402 Size: 915 714 262 2356 Fragments arranged by size: 2356 915 714 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4247 SphI G_CATG'C Cuts at: 1300 1800 1300 Size: 500 3747 StuI AGG'CCT Cuts at: 211 540 211 Size: 329 3918 StyI C'CwwG_G Cuts at: 107 471 821 1381 1735 107 Size: 364 350 560 354 2619 Fragments arranged by size: 2619 560 364 354 350 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1101 1830 1963 2422 2631 1101 Size: 729 133 459 209 2717 Fragments arranged by size: 2717 729 459 209 133 TatI w'GTAC_w Cuts at: 329 692 3762 329 Size: 363 3070 814 Fragments arranged by size: 3070 814 363 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 872 904 3764 3796 872 Size: 32 2860 32 1323 Fragments arranged by size: 2860 1323 32 32 Tth111I GACn'n_nGTC Cuts at: 2044 2044 Size: 4247 XbaI T'CTAG_A Cuts at: 1278 1278 Size: 4247 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1742 1742 Size: 4247 XmaI C'CCGG_G Cuts at: 1267 1267 Size: 4247 XmnI GAAnn'nnTTC Cuts at: 415 415 Size: 4247 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII AfeI AflII AgeI AhdI AloI AscI AvrII BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BsaXI BseRI BsiWI BsmI BspEI BsrGI BssHII BstXI BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PfoI PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI