Restriction map for pF1KB5596
##################################
# NO CUT ENZYMES (  117 enzymes) #
##################################

AatII       Acc65I      AccI        AflII       AgeI        
Alw44I      AlwNI       AocI        ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspHI       AspI        AvaI        AviII       AvrII       
BamHI       BbeI        BbrPI       BcgI        BclI        
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BsaI        BsgI        BsiHKAI     BsiWI       BsmBI       
BsoBI       BspDI       BspLU11I    BsrBI       BsrFI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstYI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        Eco81I      EcoO65I     EcoRV       
EcoT22I     FbaI        FseI        FspI        HincII      
HindII      HindIII     HpaI        HphI        KasI        
KpnI        MflI        MluI        NaeI        NarI        
NcoI        NgoMIV      NheI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       PvuI        RsrII       SacI        SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    StuI        SwaI        
Tth111I     Van91I      XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AccII         147  CGCG                 CG^CG
AccIII       1155  TCCGGA               T^CCGGA
AclI          606  AACGTT               AA^CGTT
AcyI          720  GACGCC               G[GA]^CG[CT]C
AflIII       1837  ACACGT               A^C[GA][CT]GT
AhdI         1386  GACTGGAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI      1376  AGCGCT               AGC^GCT
AspEI        1386  GACTGGAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI          944  GGTGCC               G^G[CT][GA]CC
BbvCI         263  CCTCAGC              CCTCAGC(-5/-2)
BciVI        1158  GGATAC               GTATCC(6/5)
BglI            7  GCCAATGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI         720  GACGCC               G[GA]^CG[CT]C
BsaWI        1155  TCCGGA               [AT]^CCGG[AT]
BseAI        1155  TCCGGA               T^CCGGA
BsiEI         251  CGGCCG               CG[GA][CT]^CG
Bsp1407I     1293  TGTACA               T^GTACA
BspEI        1155  TCCGGA               T^CCGGA
BspHI        1332  TCATGA               T^CATGA
BspMI        1166  ACCTGC               ACCTGC(4/8)
BsrDI        1464  GCAATG               GCAATG(2/0)
BsrGI        1293  TGTACA               T^GTACA
BstUI         147  CGCG                 CG^CG
BstXI         162  CCACGCCAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EagI          251  CGGCCG               C^GGCCG
Eam1105I     1386  GACTGGAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI         251  CGGCCG               C^GGCCG
Eco47III     1376  AGCGCT               AGC^GCT
Eco52I        251  CGGCCG               C^GGCCG
EcoNI         912  CCTGCGGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        1523  GAATTC               G^AATTC
EcoT14I       311  CCTTGG               C^C[AT][AT]GG
HgaI          720  GACGC                GACGC(5/10)
Hin1I         720  GACGCC               G[GA]^CG[CT]C
KspI          146  CCGCGG               CCGC^GG
MfeI         1814  CAATTG               C^AATTG
MroI         1155  TCCGGA               T^CCGGA
MunI         1814  CAATTG               C^AATTG
MvnI          147  CGCG                 CG^CG
NdeI         1140  CATATG               CA^TATG
NotI          250  GCGGCCGC             GC^GGCCGC
NspI         1197  ACATGC               [GA]CATG^[CT]
Psp1406I      606  AACGTT               AA^CGTT
PstI          646  CTGCAG               CTGCA^G
PvuII         278  CAGCTG               CAG^CTG
RcaI         1332  TCATGA               T^CATGA
SacII         146  CCGCGG               CCGC^GG
SfcI          646  CTGCAG               C^T[GA][CT]AG
SspBI        1293  TGTACA               T^GTACA
SspI         1951  AATATT               AAT^ATT
StyI          311  CCTTGG               C^C[AT][AT]GG
TaqI         1541  TCGA                 T^CGA
TthHB8I      1541  TCGA                 T^CGA
XbaI          978  TCTAGA               T^CTAGA
XcmI          317  CCACCCCAGAACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

BalI          314  TGGCCA               TGG^CCA
             1886  TGGCCA              
BanII         172  GGGCTC               G[GA]GC[CT]^C
              415  GGGCTC              
BcnI           87  CCCGG                CC^[GC]GG
              241  CCCGG               
BsaAI         794  TACGTG               [CT]AC^GT[GA]
             1838  CACGTA              
BsaBI         970  GATAATAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1426  GATGGTAATC          
DsaI          146  CCGCGG               C^C[GA][CT]GG
              892  CCACGG              
EarI          306  CTCTTC               CTCTTC(1/4)
             1957  GAAGAG              
HaeII        1376  AGCGCT               [GA]GCGC^[CT]
             1891  AGCGCC              
Ksp632I       306  CTCTTC               CTCTTC(1/4)
             1957  GAAGAG              
MamI          970  GATAATAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1426  GATGGTAATC          
MluNI         314  TGGCCA               TGG^CCA
             1886  TGGCCA              
MscI          314  TGGCCA               TGG^CCA
             1886  TGGCCA              
MseI         1181  TTAA                 T^TAA
             1627  TTAA                
NciI           87  CCCGG                CC^[GC]GG
              241  CCCGG               
SmlI          340  CTCAAG               C^T[CT][GA]AG
             1479  CTTGAG              
Tru9I        1181  TTAA                 T^TAA
             1627  TTAA                
Tsp45I       1384  GTGAC                ^GT[GC]AC
             1418  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   69 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AvaII       BbsI        BbvI        BfaI        
BmyI        BpmI        BpuAI       BsaJI       BseRI       
BsiYI       BslI        BsmAI       BsmFI       BsmI        
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       EaeI        EcoO109I    EcoRII      FauI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       ItaI        MaeI        
MaeII       MaeIII      MboI        MboII       MnlI        
MslI        MspA1I      MspI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       PleI        PpuMI       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
TfiI        TseI        Tsp509I     TspRI       
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