Restriction map for pF1KB5596
 (Linear) (Six-base) MAPSORT of: KIBB5596.seq  Check: 5721  from: 1  to: 1983



KIBB5596.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:38  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1175    1983 
   Size:       1175     808
 
AclI AA'CG_TT

Cuts at:      0     607    1983 
   Size:        607    1376
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     841    1983 
   Size:        841    1142
 
AfeI AGC'GCT

Cuts at:      0    1378    1983 
   Size:       1378     605
 
AflIII A'CryG_T

Cuts at:      0    1837    1983 
   Size:       1837     146
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1391    1983 
   Size:       1391     592
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1481    1513    1983 
   Size:       1481      32     470

  Fragments arranged by size: 

                1481     470      32
 
ApoI r'AATT_y

Cuts at:      0     484    1084    1523    1983 
   Size:        484     600     439     460

  Fragments arranged by size: 

                 600     484     460     439
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1118    1151    1983 
   Size:       1118      33     832

  Fragments arranged by size: 

                1118     832      33
 
BanI G'GyrC_C

Cuts at:      0     944    1983 
   Size:        944    1039
 
BanII G_rGCy'C

Cuts at:      0     176     419    1983 
   Size:        176     243    1564

  Fragments arranged by size: 

                1564     243     176
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     794    1343    1754    1983 
   Size:        794     549     411     229

  Fragments arranged by size: 

                 794     549     411     229
 
BbvCI CC'TCA_GC

Cuts at:      0     264    1983 
   Size:        264    1719
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1152    1983 
   Size:       1152     831
 
BglI GCCn_nnn'nGGC

Cuts at:      0      13    1983 
   Size:         13    1970
 
Bme1580I G_kGCm'C

Cuts at:      0    1835    1983 
   Size:       1835     148
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     337     357     522    1983 
   Size:        337      20     165    1461

  Fragments arranged by size: 

                1461     337     165      20
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1346    1983 
   Size:       1346     637
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     691     709    1409    1983 
   Size:        691      18     700     574

  Fragments arranged by size: 

                 700     691     574      18
 
Bpu10I CC'TnA_GC

Cuts at:      0     264     411    1983 
   Size:        264     147    1572

  Fragments arranged by size: 

                1572     264     147
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     325    1500    1983 
   Size:        325    1175     483

  Fragments arranged by size: 

                1175     483     325
 
BsaAI yAC'GTr

Cuts at:      0     796    1840    1983 
   Size:        796    1044     143

  Fragments arranged by size: 

                1044     796     143
 
BsaBI GATnn'nnATC

Cuts at:      0     974    1430    1983 
   Size:        974     456     553

  Fragments arranged by size: 

                 974     553     456
 
BsaHI Gr'CG_yC

Cuts at:      0     721    1983 
   Size:        721    1262
 
BsaWI w'CCGG_w

Cuts at:      0    1155    1983 
   Size:       1155     828
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     893     923    1983 
   Size:        893      30    1060

  Fragments arranged by size: 

                1060     893      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      35      38      41      44      68      71     294     427 
   Size:         35       3       3       3      24       3     223     133

Cuts at:    427     554    1983 
   Size:        127    1429

  Fragments arranged by size: 

                1429     223     133     127      35      24       3       3
                   3       3
 
BsiEI CG_ry'CG

Cuts at:      0     254    1983 
   Size:        254    1729
 
BsmI GAATG_Cn'

Cuts at:      0     558     746    1969    1983 
   Size:        558     188    1223      14

  Fragments arranged by size: 

                1223     558     188      14
 
Bsp1286I G_dGCh'C

Cuts at:      0     176     419    1835    1983 
   Size:        176     243    1416     148

  Fragments arranged by size: 

                1416     243     176     148
 
BspEI T'CCGG_A

Cuts at:      0    1155    1983 
   Size:       1155     828
 
BspHI T'CATG_A

Cuts at:      0    1332    1983 
   Size:       1332     651
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1175    1983 
   Size:       1175     808
 
BsrDI GCAATG_nn'

Cuts at:      0    1471    1983 
   Size:       1471     512
 
BsrGI T'GTAC_A

Cuts at:      0    1293    1983 
   Size:       1293     690
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     169    1983 
   Size:        169    1814
 
BtgI C'CryG_G

Cuts at:      0     146     892    1983 
   Size:        146     746    1091

  Fragments arranged by size: 

                1091     746     146
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1740    1983 
   Size:       1740     243
 
BtsI GCAGTG_nn'

Cuts at:      0    1773    1983 
   Size:       1773     210
 
EaeI y'GGCC_r

Cuts at:      0     251     314    1611    1886    1983 
   Size:        251      63    1297     275      97

  Fragments arranged by size: 

                1297     275     251      97      63
 
EagI C'GGCC_G

Cuts at:      0     251    1983 
   Size:        251    1732
 
EarI CTCTTCn'nnn_

Cuts at:      0     312    1952    1983 
   Size:        312    1640      31

  Fragments arranged by size: 

                1640     312      31
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     174     183     276    1983 
   Size:        174       9      93    1707

  Fragments arranged by size: 

                1707     174      93       9
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     691     709     841    1409    1983 
   Size:        691      18     132     568     574

  Fragments arranged by size: 

                 691     574     568     132      18
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     916    1983 
   Size:        916    1067
 
EcoO109I rG'GnC_Cy

Cuts at:      0     153     684    1545    1983 
   Size:        153     531     861     438

  Fragments arranged by size: 

                 861     531     438     153
 
EcoRI G'AATT_C

Cuts at:      0    1523    1983 
   Size:       1523     460
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1373    1983 
   Size:       1373     610
 
HaeII r_GCGC'y

Cuts at:      0    1380    1895    1983 
   Size:       1380     515      88

  Fragments arranged by size: 

                1380     515      88
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     417     449     973    1005    1255    1287    1377    1409 
   Size:        417      32     524      32     250      32      90      32

Cuts at:   1409    1983 
   Size:        574

  Fragments arranged by size: 

                 574     524     417     250      90      32      32      32
                  32
 
MfeI C'AATT_G

Cuts at:      0    1814    1983 
   Size:       1814     169
 
MscI TGG'CCA

Cuts at:      0     316    1888    1983 
   Size:        316    1572      95

  Fragments arranged by size: 

                1572     316      95
 
MslI CAynn'nnrTG

Cuts at:      0     167     404    1064    1202    1983 
   Size:        167     237     660     138     781

  Fragments arranged by size: 

                 781     660     237     167     138
 
MspA1I CmG'CkG

Cuts at:      0     148     280     726    1983 
   Size:        148     132     446    1257

  Fragments arranged by size: 

                1257     446     148     132
 
NdeI CA'TA_TG

Cuts at:      0    1141    1983 
   Size:       1141     842
 
NotI GC'GGCC_GC

Cuts at:      0     251    1983 
   Size:        251    1732
 
NspI r_CATG'y

Cuts at:      0    1201    1983 
   Size:       1201     782
 
PfoI T'CCnGG_A

Cuts at:      0     686    1983 
   Size:        686    1297
 
PpuMI rG'GwC_Cy

Cuts at:      0     153     684    1545    1983 
   Size:        153     531     861     438

  Fragments arranged by size: 

                 861     531     438     153
 
PstI C_TGCA'G

Cuts at:      0     650    1983 
   Size:        650    1333
 
PvuII CAG'CTG

Cuts at:      0     280    1983 
   Size:        280    1703
 
SacII CC_GC'GG

Cuts at:      0     149    1983 
   Size:        149    1834
 
SfcI C'TryA_G

Cuts at:      0     646    1983 
   Size:        646    1337
 
SmlI C'TyrA_G

Cuts at:      0     340    1479    1983 
   Size:        340    1139     504

  Fragments arranged by size: 

                1139     504     340
 
SspI AAT'ATT

Cuts at:      0    1953    1983 
   Size:       1953      30
 
StyI C'CwwG_G

Cuts at:      0     311    1983 
   Size:        311    1672
 
TatI w'GTAC_w

Cuts at:      0    1293    1566    1983 
   Size:       1293     273     417

  Fragments arranged by size: 

                1293     417     273
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     608     640    1983 
   Size:        608      32    1343

  Fragments arranged by size: 

                1343     608      32
 
XbaI T'CTAG_A

Cuts at:      0     978    1983 
   Size:        978    1005
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     324    1983 
   Size:        324    1659
 

 Enzymes that do cut:

     AarI     AclI     AcuI     AfeI   AflIII     AhdI     AloI     ApoI
     BaeI     BanI    BanII     BbsI    BbvCI    BciVI     BglI Bme1580I
     BmrI     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BsiEI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrDI    BsrGI    BstXI     BtgI    BtgZI     BtsI     EaeI
     EagI     EarI     EciI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI
    HaeII    Hin4I     MfeI     MscI     MslI   MspA1I     NdeI     NotI
     NspI     PfoI    PpuMI     PstI    PvuII    SacII     SfcI     SmlI
     SspI     StyI     TatI     TstI     XbaI     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I    AflII     AgeI     AleI     AlfI    AlwNI
     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII    BamHI
     BbeI     BcgI     BclI    BfrBI    BglII     BlpI    BmgBI     BmtI
     BsaI    BseYI     BsgI  BsiHKAI    BsiWI    BsmBI    BsrBI    BsrFI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I
     ClaI    CspCI     DraI   DraIII     DrdI  EcoICRI    EcoRV     FseI
     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MluI
     MmeI     NaeI     NarI     NcoI   NgoMIV     NheI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI
    TaqII  Tth111I     XhoI     XmaI     XmnI     ZraI
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