Restriction map for pF1KB5513
##################################
# NO CUT ENZYMES (   98 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcyI        
AflII       AgeI        AhdI        Alw44I      ApaLI       
AscI        AseI        AsnI        AspEI       AspI        
AviII       BalI        BbeI        BbrPI       BcgI        
BciVI       BfrI        BlpI        Bpu1102I    BsaAI       
BsaHI       BsaI        BseAI       BsiWI       BsmBI       
Bsp1407I    BspEI       BspHI       BspLU11I    BsrFI       
BsrGI       BssHII      Bst1107I    BstXI       BstZ17I     
CelII       Cfr10I      CpoI        DraI        DraIII      
DrdI        Eam1105I    EcoT22I     FseI        FspI        
Hin1I       HindIII     HpaI        KasI        MfeI        
MluNI       MroI        MscI        MunI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        PacI        PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SalI        SbfI        SexAI       SfiI        
SfoI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tth111I     Van91I      XbaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

Acc65I       1858  GGTACC               G^GTACC
AflIII        785  ACGCGT               A^C[GA][CT]GT
AocI          993  CCTGAGG              CC^T[ACGT]AGG
Aor51HI      1379  AGCGCT               AGC^GCT
ApaI          190  GGGCCC               GGGCC^C
Asp718I      1858  GGTACC               G^GTACC
AvrII         303  CCTAGG               C^CTAGG
BamHI        2037  GGATCC               G^GATCC
BbsI          613  GAAGAC               GAAGAC(2/6)
BbvCI        1341  CCTCAGC              CCTCAGC(-5/-2)
BclI         1163  TGATCA               T^GATCA
BlnI          303  CCTAGG               C^CTAGG
BpuAI         613  GAAGAC               GAAGAC(2/6)
BsaBI         843  GATCCGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI         1212  CTGCAC               GTGCAG(16/14)
BsiEI          41  CGGCCG               CG[GA][CT]^CG
BsmI          504  GCATTC               GAATGC(1/-1)
BspDI        1969  ATCGAT               AT^CGAT
BspMI        1900  GCAGGT               ACCTGC(4/8)
BsrBI        1206  GAGCGG               CCGCTC(-3/-3)
BstAPI        325  GCAGCCATTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        795  GGTCACC              G^GT[ACGT]ACC
BstPI         795  GGTCACC              G^GT[ACGT]ACC
Bsu36I        993  CCTGAGG              CC^T[ACGT]AGG
ClaI         1969  ATCGAT               AT^CGAT
EagI           41  CGGCCG               C^GGCCG
EclXI          41  CGGCCG               C^GGCCG
Eco47III     1379  AGCGCT               AGC^GCT
Eco52I         41  CGGCCG               C^GGCCG
Eco81I        993  CCTGAGG              CC^T[ACGT]AGG
EcoO65I       795  GGTCACC              G^GT[ACGT]ACC
EcoRV        1696  GATATC               GAT^ATC
FbaI         1163  TGATCA               T^GATCA
HgaI          129  GACGC                GACGC(5/10)
KpnI         1858  GGTACC               GGTAC^C
MamI          843  GATCCGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI          785  ACGCGT               A^CGCGT
PaeR7I        365  CTCGAG               C^TCGAG
PpuMI         140  AGGACCC              [GA]G^G[AT]CC[CT]
PstI         1657  CTGCAG               CTGCA^G
PvuII        2032  CAGCTG               CAG^CTG
SacI         2062  GAGCTC               GAGCT^C
SapI          132  GCTCTTC              GCTCTTC(1/4)
ScaI         2094  AGTACT               AGT^ACT
SmaI          209  CCCGGG               CCC^GGG
SmlI          365  CTCGAG               C^T[CT][GA]AG
XcmI          255  CCAGTTTATCATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          365  CTCGAG               C^TCGAG
XmaCI         209  CCCGGG               C^CCGGG
XmaI          209  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

Asp700I      1134  GAACACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1782  GAAGGAGTTC          
AspHI        1411  GTGCTC               G[AT]GC[AT]^C
             2062  GAGCTC              
AvaII          30  GGACC                G^G[AT]CC
              141  GGACC               
BanI          592  GGTGCC               G^G[CT][GA]CC
             1858  GGTACC              
BglI           16  GCCAAGCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1615  GCCCAGTTGGC         
BsaWI         622  ACCGGA               [AT]^CCGG[AT]
              799  ACCGGA              
BsiHKAI      1411  GTGCTC               G[AT]GC[AT]^C
             2062  GAGCTC              
BsmAI        1605  GAGAC                GTCTC(1/5)
             1723  GAGAC               
BsmFI         127  GGGAC                GGGAC(10/14)
              373  GTCCC               
BsrDI         330  CATTGC               GCAATG(2/0)
              630  CATTGC              
BssSI          61  CACGAG               CACGAG(-5/-1)
             1450  CTCGTG              
BstBI         610  TTCGAA               TT^CGAA
             1465  TTCGAA              
EarI          133  CTCTTC               CTCTTC(1/4)
             1561  GAAGAG              
EcoNI          81  CCTCAACCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1401  CCTCCGGGAGG         
EcoRI         967  GAATTC               G^AATTC
             1866  GAATTC              
Ksp632I       133  CTCTTC               CTCTTC(1/4)
             1561  GAAGAG              
KspI          740  CCGCGG               CCGC^GG
             1109  CCGCGG              
MaeII         760  ACGT                 A^CGT
             1844  ACGT                
MseI         1318  TTAA                 T^TAA
             2027  TTAA                
NspV          610  TTCGAA               TT^CGAA
             1465  TTCGAA              
SacII         740  CCGCGG               CCGC^GG
             1109  CCGCGG              
SfuI          610  TTCGAA               TT^CGAA
             1465  TTCGAA              
Tru9I        1318  TTAA                 T^TAA
             2027  TTAA                
XmnI         1134  GAACACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1782  GAAGGAGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   86 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        AvaI        BanII       
BbvI        BcnI        BfaI        BglII       BmyI        
BpmI        BsaJI       BseRI       BsiYI       BslI        
BsoBI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstUI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        HinP1I      HincII      HindII      HinfI       
HpaII       HphI        ItaI        MaeI        MaeIII      
MboI        MboII       MflI        MnlI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PleI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StyI        TaqI        TfiI        
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII       
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