Restriction map for
pF1KB5378
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# NO CUT ENZYMES ( 117 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BbeI BbrPI BbvCI BciVI
BclI BfrI BlnI BsaBI BsaHI
BseAI BsiEI BsiWI BsmI BsoBI
BspDI BspEI BspHI BsrBI BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRV FbaI FseI FspI HaeII
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MluI
MroI MvnI NaeI NarI NgoMIV
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tth111I XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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Alw44I 216 GTGCAC G^TGCAC
AlwNI 1261 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 333 CCTCAGG CC^T[ACGT]AGG
ApaLI 216 GTGCAC G^TGCAC
BalI 254 TGGCCA TGG^CCA
BbsI 1172 GTCTTC GAAGAC(2/6)
BcgI 1057 CGAGGGTACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 1270 CCCGG CC^[GC]GG
BglI 1708 GCCCACTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1905 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 1905 GCTTAGC GC^T[ACGT]AGC
BpuAI 1172 GTCTTC GAAGAC(2/6)
BsaAI 802 CACGTA [CT]AC^GT[GA]
BsaWI 86 ACCGGA [AT]^CCGG[AT]
BsmBI 2152 GAGACG CGTCTC(1/5)
Bsp1407I 209 TGTACA T^GTACA
BsrGI 209 TGTACA T^GTACA
Bsu36I 333 CCTCAGG CC^T[ACGT]AGG
CelII 1905 GCTTAGC GC^T[ACGT]AGC
Eco81I 333 CCTCAGG CC^T[ACGT]AGG
EcoNI 401 CCTATGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 439 ATGCAT ATGCA^T
HgaI 2154 GACGC GACGC(5/10)
MluNI 254 TGGCCA TGG^CCA
MscI 254 TGGCCA TGG^CCA
MspA1I 1409 CAGCTG C[AC]G^C[GT]G
NciI 1270 CCCGG CC^[GC]GG
NdeI 221 CATATG CA^TATG
NheI 843 GCTAGC G^CTAGC
NsiI 439 ATGCAT ATGCA^T
PflMI 1693 CCAGTGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1537 GACCGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1409 CAGCTG CAG^CTG
SacI 871 GAGCTC GAGCT^C
SapI 1978 GCTCTTC GCTCTTC(1/4)
SspBI 209 TGTACA T^GTACA
Van91I 1693 CCAGTGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 546 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflIII 134 ACATGT A^C[GA][CT]GT
212 ACATGT
Asp700I 1342 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1399 GAAACATTTC
BamHI 513 GGATCC G^GATCC
1638 GGATCC
BanI 472 GGTGCC G^G[CT][GA]CC
1689 GGTGCC
BpmI 1969 CTCCAG CTGGAG(16/14)
1844 CTGGAG
BseRI 1561 CTCCTC GAGGAG(10/8)
1048 GAGGAG
BspLU11I 134 ACATGT A^CATGT
212 ACATGT
BspMI 1461 ACCTGC ACCTGC(4/8)
1038 GCAGGT
BstXI 290 CCAATCCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1193 CCACAGTGGTGG
CfoI 81 GCGC GCG^C
1443 GCGC
DsaI 1335 CCATGG C^C[GA][CT]GG
2032 CCATGG
EaeI 254 TGGCCA [CT]^GGCC[GA]
2029 CGGCCA
EcoRI 1346 GAATTC G^AATTC
1951 GAATTC
HhaI 81 GCGC GCG^C
1443 GCGC
HinP1I 81 GCGC G^CGC
1443 GCGC
MfeI 518 CAATTG C^AATTG
2198 CAATTG
MunI 518 CAATTG C^AATTG
2198 CAATTG
NcoI 1335 CCATGG C^CATGG
2032 CCATGG
PleI 120 GACTC GAGTC(4/5)
457 GACTC
PpuMI 834 GGGACCT [GA]G^G[AT]CC[CT]
1791 AGGACCC
SexAI 921 ACCAGGT A^CC[AT]GGT
1220 ACCTGGT
SfcI 176 CTGTAG C^T[GA][CT]AG
988 CTACAG
XmnI 1342 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1399 GAAACATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BanII BbvI
BfaI BglII BmyI BsaI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NdeII NlaIII NlaIV NspI RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII