Restriction map for
pF1KB5378
(Circular) (Six-base) MAPSORT of: pF1KB5378.seq Check: 3460 from: 1 to: 5330
pF1KB5378 5330 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1137 1137
Size: 5330
AccI GT'mk_AC
Cuts at: 2368 2368
Size: 5330
Acc65I G'GTAC_C
Cuts at: 2346 2346
Size: 5330
AclI AA'CG_TT
Cuts at: 5170 5170
Size: 5330
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 590 776 950 1376 1934 3155 3587 3940
Size: 424 186 174 426 558 1221 432 353
Cuts at: 3940 4602 166
Size: 662 894
Fragments arranged by size:
1221 894 662 558 432 426 424 353
186 174
AflIII A'CryG_T
Cuts at: 242 320 2682 4482 4763 242
Size: 78 2362 1800 281 809
Fragments arranged by size:
2362 1800 809 281 78
AleI CACnn'nnGTG
Cuts at: 1180 1306 1795 1180
Size: 126 489 4715
Fragments arranged by size:
4715 489 126
AlwNI CAG_nnn'CTG
Cuts at: 1374 2635 4073 1374
Size: 1261 1438 2631
Fragments arranged by size:
2631 1438 1261
ApaLI G'TGCA_C
Cuts at: 324 4168 324
Size: 3844 1486
ApoI r'AATT_y
Cuts at: 662 1454 2059 2334 4683 4939 662
Size: 792 605 275 2349 256 1053
Fragments arranged by size:
2349 1053 792 605 275 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5330
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5330
AvaI C'yCGr_G
Cuts at: 2350 2350
Size: 5330
BamHI G'GATC_C
Cuts at: 621 1746 2355 621
Size: 1125 609 3596
Fragments arranged by size:
3596 1125 609
BanI G'GyrC_C
Cuts at: 580 1797 2346 2760 3008 3043 580
Size: 1217 549 414 248 35 2867
Fragments arranged by size:
2867 1217 549 414 248 35
BanII G_rGCy'C
Cuts at: 983 1082 1959 2344 3374 983
Size: 99 877 385 1030 2939
Fragments arranged by size:
2939 1030 877 385 99
BbeI G_GCGC'C
Cuts at: 2764 3012 2764
Size: 248 5082
BbsI GAAGACnn'nnnn_
Cuts at: 1273 1273
Size: 5330
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1154 1188 90
Size: 34 1030 34 4232
Fragments arranged by size:
4232 1030 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3228 4284 5330 3228
Size: 1056 1046 3228
Fragments arranged by size:
3228 1056 1046
BclI T'GATC_A
Cuts at: 2850 2850
Size: 5330
BfrBI ATG'CAT
Cuts at: 549 549
Size: 5330
BglI GCCn_nnn'nGGC
Cuts at: 1822 1822
Size: 5330
BglII A'GATC_T
Cuts at: 348 1013 2228 2845 4882 348
Size: 665 1215 617 2037 796
Fragments arranged by size:
2037 1215 796 665 617
BlpI GC'TnA_GC
Cuts at: 2014 2442 2014
Size: 428 4902
Bme1580I G_kGCm'C
Cuts at: 328 585 2955 3048 4172 328
Size: 257 2370 93 1124 1486
Fragments arranged by size:
2370 1486 1124 257 93
BmgBI CAC'GTC
Cuts at: 132 132
Size: 5330
BmrI ACTGGGnnnn_n'
Cuts at: 1830 2542 2623 2958 5102 1830
Size: 712 81 335 2144 2058
Fragments arranged by size:
2144 2058 712 335 81
BmtI G_CTAG'C
Cuts at: 955 955
Size: 5330
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3697 3697
Size: 5330
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1973 2062 1973
Size: 89 5241
Bpu10I CC'TnA_GC
Cuts at: 2162 2162
Size: 5330
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 215 666 823 2506 3385 4099 4397 215
Size: 451 157 1683 879 714 298 1148
Fragments arranged by size:
1683 1148 879 714 451 298 157
BsaI GGTCTCn'nnnn_
Cuts at: 656 1319 1657 2138 5034 656
Size: 663 338 481 2896 952
Fragments arranged by size:
2896 952 663 481 338
BsaAI yAC'GTr
Cuts at: 912 2683 912
Size: 1771 3559
BsaBI GATnn'nnATC
Cuts at: 6 2844 4881 6
Size: 2838 2037 455
Fragments arranged by size:
2838 2037 455
BsaHI Gr'CG_yC
Cuts at: 2761 3009 3711 5207 2761
Size: 248 702 1496 2884
Fragments arranged by size:
2884 1496 702 248
BsaWI w'CCGG_w
Cuts at: 194 2519 3040 3718 4129 4276 4550 194
Size: 2325 521 678 411 147 274 974
Fragments arranged by size:
2325 974 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 106 136 318 348 420 450 671 701 1099
Size: 30 182 30 72 30 221 30 398
Cuts at: 1099 1129 1925 1955 106
Size: 30 796 30 3481
Fragments arranged by size:
3481 796 398 221 182 72 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1171 1660 1171
Size: 489 4841
BseYI C'CCAG_C
Cuts at: 1275 1772 2128 2755 3513 4178 4586 1275
Size: 497 356 627 758 665 408 2019
Fragments arranged by size:
2019 758 665 627 497 408 356
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 478 1158 1162 1333 478
Size: 680 4 171 4475
Fragments arranged by size:
4475 680 171 4
BsiEI CG_ry'CG
Cuts at: 104 2872 2918 4148 104
Size: 2768 46 1230 1286
Fragments arranged by size:
2768 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 328 983 1295 2163 2344 3122 3312 4172 328
Size: 655 312 868 181 778 190 860 1486
Fragments arranged by size:
1486 868 860 778 655 312 190 181
BsmBI CGTCTCn'nnnn_
Cuts at: 2254 2254
Size: 5330
Bsp1286I G_dGCh'C
Cuts at: 328 585 983 1082 1295 1959 2163 2344 2955
Size: 257 398 99 213 664 204 181 611
Cuts at: 2955 3048 3122 3312 3374 4172 328
Size: 93 74 190 62 798 1486
Fragments arranged by size:
1486 798 664 611 398 257 213 204
190 181 99 93 74 62
BspHI T'CATG_A
Cuts at: 3762 3762
Size: 5330
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1137 1578 2380 2594 2896 3277 1137
Size: 441 802 214 302 381 3190
Fragments arranged by size:
3190 802 441 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3622 3676 5160 5325 50
Size: 3572 54 1484 165 55
Fragments arranged by size:
3572 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 701 1226 2123 3242 701
Size: 525 897 1119 2789
Fragments arranged by size:
2789 1119 897 525
BsrFI r'CCGG_y
Cuts at: 3328 3509 3328
Size: 181 5149
BsrGI T'GTAC_A
Cuts at: 317 317
Size: 5330
BssSI C'ACGA_G
Cuts at: 3601 4309 3601
Size: 708 4622
BstAPI GCAn_nnn'nTGC
Cuts at: 386 1149 1204 2043 2707 386
Size: 763 55 839 664 3009
Fragments arranged by size:
3009 839 763 664 55
BstBI TT'CG_AA
Cuts at: 3691 3691
Size: 5330
BstXI CCAn_nnnn'nTGG
Cuts at: 405 1308 405
Size: 903 4427
BstYI r'GATC_y
Cuts at: 1 348 621 1013 1746 2228 2355 2822 2845
Size: 347 273 392 733 482 127 467 23
Cuts at: 2845 3180 3426 3732 3744 3830 4882 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 733 482 467 449 392 347 335
306 273 246 127 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 442 442
Size: 5330
BtgI C'CryG_G
Cuts at: 1443 2140 1443
Size: 697 4633
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2097 3280 3461 3624 2097
Size: 1183 181 163 3803
Fragments arranged by size:
3803 1183 181 163
BtsI GCAGTG_nn'
Cuts at: 798 1538 2573 798
Size: 740 1035 3555
Fragments arranged by size:
3555 1035 740
DraI TTT'AAA
Cuts at: 2329 2329
Size: 5330
DrdI GACnn_nn'nnGTC
Cuts at: 3036 4380 3036
Size: 1344 3986
EaeI y'GGCC_r
Cuts at: 362 2137 2915 3089 3480 3507 4852 362
Size: 1775 778 174 391 27 1345 840
Fragments arranged by size:
1775 1345 840 778 391 174 27
EagI C'GGCC_G
Cuts at: 2915 2915
Size: 5330
EarI CTCTTCn'nnn_
Cuts at: 1382 1660 1910 2093 3353 3563 1382
Size: 278 250 183 1260 210 3149
Fragments arranged by size:
3149 1260 278 250 210 183
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3538 4280 4426 5140 3538
Size: 742 146 714 3728
Fragments arranged by size:
3728 742 714 146
EcoICRI GAG'CTC
Cuts at: 981 2342 981
Size: 1361 3969
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 590 776 950 1376 1934 1973 2062 3155
Size: 424 186 174 426 558 39 89 1093
Cuts at: 3155 3587 3940 4602 166
Size: 432 353 662 894
Fragments arranged by size:
1093 894 662 558 432 426 424 353
186 174 89 39
EcoNI CCTnn'n_nnAGG
Cuts at: 513 513
Size: 5330
EcoO109I rG'GnC_Cy
Cuts at: 943 1371 1900 2469 943
Size: 428 529 569 3804
Fragments arranged by size:
3804 569 529 428
EcoRI G'AATT_C
Cuts at: 1454 2059 2334 1454
Size: 605 275 4450
Fragments arranged by size:
4450 605 275
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3349 3349
Size: 5330
FspI TGC'GCA
Cuts at: 3111 3111
Size: 5330
HaeII r_GCGC'y
Cuts at: 2764 3012 4242 5013 2764
Size: 248 1230 771 3081
Fragments arranged by size:
3081 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 512 537 544 569 671 703 3172 3202 3204
Size: 25 7 25 102 32 2469 30 2
Cuts at: 3204 3234 512
Size: 30 2608
Fragments arranged by size:
2608 2469 102 32 30 30 25 25
7 2
HincII GTy'rAC
Cuts at: 2369 2369
Size: 5330
KasI G'GCGC_C
Cuts at: 2760 3008 2760
Size: 248 5082
KpnI G_GTAC'C
Cuts at: 2350 2350
Size: 5330
MfeI C'AATT_G
Cuts at: 626 2306 626
Size: 1680 3650
MluI A'CGCG_T
Cuts at: 4763 4763
Size: 5330
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 806 1484 4089 4273 806
Size: 678 2605 184 1863
Fragments arranged by size:
2605 1863 678 184
MscI TGG'CCA
Cuts at: 364 3091 364
Size: 2727 2603
MslI CAynn'nnrTG
Cuts at: 1180 1306 1795 2116 3319 3446 1180
Size: 126 489 321 1203 127 3064
Fragments arranged by size:
3064 1203 489 321 127 126
MspA1I CmG'CkG
Cuts at: 1519 2441 2755 3115 3899 4144 1519
Size: 922 314 360 784 245 2705
Fragments arranged by size:
2705 922 784 360 314 245
NaeI GCC'GGC
Cuts at: 3511 3511
Size: 5330
NarI GG'CG_CC
Cuts at: 2761 3009 2761
Size: 248 5082
NcoI C'CATG_G
Cuts at: 1443 2140 1443
Size: 697 4633
NdeI CA'TA_TG
Cuts at: 330 330
Size: 5330
NgoMIV G'CCGG_C
Cuts at: 3509 3509
Size: 5330
NheI G'CTAG_C
Cuts at: 951 951
Size: 5330
NsiI A_TGCA'T
Cuts at: 551 551
Size: 5330
NspI r_CATG'y
Cuts at: 246 324 435 1590 2383 2883 4486 246
Size: 78 111 1155 793 500 1603 1090
Fragments arranged by size:
1603 1155 1090 793 500 111 78
PciI A'CATG_T
Cuts at: 242 320 4482 242
Size: 78 4162 1090
Fragments arranged by size:
4162 1090 78
PflMI CCAn_nnn'nTGG
Cuts at: 1807 1807
Size: 5330
PmeI GTTT'AAAC
Cuts at: 2329 2329
Size: 5330
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3776 3808 3776
Size: 32 5298
PpuMI rG'GwC_Cy
Cuts at: 943 1900 943
Size: 957 4373
PshAI GACnn'nnGTC
Cuts at: 1649 1649
Size: 5330
PstI C_TGCA'G
Cuts at: 2377 3062 2377
Size: 685 4645
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5330
PvuII CAG'CTG
Cuts at: 1519 2755 3115 1519
Size: 1236 360 3734
Fragments arranged by size:
3734 1236 360
RsrII CG'GwC_CG
Cuts at: 3525 3525
Size: 5330
SacI G_AGCT'C
Cuts at: 983 2344 983
Size: 1361 3969
SalI G'TCGA_C
Cuts at: 2367 2367
Size: 5330
SapI GCTCTTCn'nnn_
Cuts at: 2093 3353 3563 2093
Size: 1260 210 3860
Fragments arranged by size:
3860 1260 210
SbfI CC_TGCA'GG
Cuts at: 2377 2377
Size: 5330
ScaI AGT'ACT
Cuts at: 4847 4847
Size: 5330
SexAI A'CCwGG_T
Cuts at: 1029 1328 1029
Size: 299 5031
SfcI C'TryA_G
Cuts at: 33 284 1096 2373 3058 4026 4217 33
Size: 251 812 1277 685 968 191 1146
Fragments arranged by size:
1277 1146 968 812 685 251 191
SfoI GGC'GCC
Cuts at: 2762 3010 2762
Size: 248 5082
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5330
SmaI CCC'GGG
Cuts at: 2352 2352
Size: 5330
SmlI C'TyrA_G
Cuts at: 230 645 838 2485 3400 4114 4376 230
Size: 415 193 1647 915 714 262 1184
Fragments arranged by size:
1647 1184 915 714 415 262 193
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5330
SphI G_CATG'C
Cuts at: 2383 2883 2383
Size: 500 4830
StyI C'CwwG_G
Cuts at: 117 531 1443 2047 2140 2464 2818 117
Size: 414 912 604 93 324 354 2629
Fragments arranged by size:
2629 912 604 414 354 324 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1661 2913 3046 3505 3714 1661
Size: 1252 133 459 209 3277
Fragments arranged by size:
3277 1252 459 209 133
TatI w'GTAC_w
Cuts at: 317 1351 4845 317
Size: 1034 3494 802
Fragments arranged by size:
3494 1034 802
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 318 350 438 470 1099 1131 4847 4879 318
Size: 32 88 32 629 32 3716 32 769
Fragments arranged by size:
3716 769 629 88 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3127 3127
Size: 5330
XbaI T'CTAG_A
Cuts at: 654 2361 654
Size: 1707 3623
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2825 2825
Size: 5330
XmaI C'CCGG_G
Cuts at: 2350 2350
Size: 5330
XmnI GAAnn'nnTTC
Cuts at: 1454 1511 1454
Size: 57 5273
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NsiI NspI PciI PflMI PmeI
PpiI PpuMI PshAI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BsiWI BsmI BspEI BssHII
BstEII BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI NotI NruI PacI PasI PfoI PmlI
PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI