Restriction map for
pF1KB5285
(Circular) (Six-base) MAPSORT of: pF1KB5285.seq Check: 1062 from: 1 to: 5294
pF1KB5285 5294 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 782 1620 2332 782
Size: 838 712 3744
Fragments arranged by size:
3744 838 712
Acc65I G'GTAC_C
Cuts at: 1366 2310 1366
Size: 944 4350
AclI AA'CG_TT
Cuts at: 5134 5134
Size: 5294
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 353 430 479 493 611 3119 3551 4298
Size: 49 77 49 14 118 2508 432 747
Cuts at: 4298 4566 304
Size: 268 1032
Fragments arranged by size:
2508 1032 747 432 268 118 77 49
49 14
AfeI AGC'GCT
Cuts at: 1543 1588 1738 1543
Size: 45 150 5099
Fragments arranged by size:
5099 150 45
AflIII A'CryG_T
Cuts at: 926 928 1801 2646 3750 4727 926
Size: 2 873 845 1104 977 1493
Fragments arranged by size:
1493 1104 977 873 845 2
AgeI A'CCGG_T
Cuts at: 3682 3682
Size: 5294
AleI CACnn'nnGTG
Cuts at: 990 990
Size: 5294
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1103 1135 1103
Size: 32 5262
AlwNI CAG_nnn'CTG
Cuts at: 370 1688 2188 2599 4166 370
Size: 1318 500 411 1567 1498
Fragments arranged by size:
1567 1498 1318 500 411
ApaLI G'TGCA_C
Cuts at: 4064 4064
Size: 5294
ApoI r'AATT_y
Cuts at: 945 1414 2298 4647 4903 945
Size: 469 884 2349 256 1336
Fragments arranged by size:
2349 1336 884 469 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5294
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5294
AvaI C'yCGr_G
Cuts at: 2260 2314 2260
Size: 54 5240
BamHI G'GATC_C
Cuts at: 2319 2319
Size: 5294
BanI G'GyrC_C
Cuts at: 1366 2310 2724 2972 3007 1366
Size: 944 414 248 35 3653
Fragments arranged by size:
3653 944 414 248 35
BanII G_rGCy'C
Cuts at: 2195 2308 3338 2195
Size: 113 1030 4151
Fragments arranged by size:
4151 1030 113
BbeI G_GCGC'C
Cuts at: 2728 2976 2728
Size: 248 5046
BbsI GAAGACnn'nnnn_
Cuts at: 334 904 2135 334
Size: 570 1231 3493
Fragments arranged by size:
3493 1231 570
BbvCI CC'TCA_GC
Cuts at: 2021 2272 2021
Size: 251 5043
BciVI GTATCCnnnnn_n'
Cuts at: 3192 3953 5294 3192
Size: 761 1341 3192
Fragments arranged by size:
3192 1341 761
BclI T'GATC_A
Cuts at: 1637 2814 1637
Size: 1177 4117
BglI GCCn_nnn'nGGC
Cuts at: 1850 1850
Size: 5294
BglII A'GATC_T
Cuts at: 1959 2809 4846 1959
Size: 850 2037 2407
Fragments arranged by size:
2407 2037 850
BlpI GC'TnA_GC
Cuts at: 2189 2406 2189
Size: 217 5077
Bme1580I G_kGCm'C
Cuts at: 349 1081 2919 3012 4068 349
Size: 732 1838 93 1056 1575
Fragments arranged by size:
1838 1575 1056 732 93
BmgBI CAC'GTC
Cuts at: 1804 1804
Size: 5294
BmrI ACTGGGnnnn_n'
Cuts at: 2506 2587 2922 5066 2506
Size: 81 335 2144 2734
Fragments arranged by size:
2734 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3661 3661
Size: 5294
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 341 1250 341
Size: 909 4385
Bpu10I CC'TnA_GC
Cuts at: 2009 2021 2272 2009
Size: 12 251 5031
Fragments arranged by size:
5031 251 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2470 3349 3841 4139 2470
Size: 879 492 298 3625
Fragments arranged by size:
3625 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 975 2243 4998 975
Size: 1268 2755 1271
Fragments arranged by size:
2755 1271 1268
BsaAI yAC'GTr
Cuts at: 929 2647 929
Size: 1718 3576
BsaBI GATnn'nnATC
Cuts at: 6 2808 4845 6
Size: 2802 2037 455
Fragments arranged by size:
2802 2037 455
BsaHI Gr'CG_yC
Cuts at: 189 2725 2973 3675 5171 189
Size: 2536 248 702 1496 312
Fragments arranged by size:
2536 1496 702 312 248
BsaWI w'CCGG_w
Cuts at: 2483 3004 3682 3956 4103 4514 2483
Size: 521 678 274 147 411 3263
Fragments arranged by size:
3263 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 339 369 1103 1133 339
Size: 30 734 30 4500
Fragments arranged by size:
4500 734 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 154 769 1886 1996 1999 2227 154
Size: 615 1117 110 3 228 3221
Fragments arranged by size:
3221 1117 615 228 110 3
BseYI C'CCAG_C
Cuts at: 2034 2219 2719 3477 4054 4550 2034
Size: 185 500 758 577 496 2778
Fragments arranged by size:
2778 758 577 500 496 185
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1269 1278 1269
Size: 9 5285
BsiEI CG_ry'CG
Cuts at: 104 2836 2882 4090 104
Size: 2732 46 1208 1308
Fragments arranged by size:
2732 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2308 3086 3276 4068 2308
Size: 778 190 792 3534
Fragments arranged by size:
3534 792 778 190
BsmI GAATG_Cn'
Cuts at: 1012 1485 1012
Size: 473 4821
BsmBI CGTCTCn'nnnn_
Cuts at: 180 180
Size: 5294
Bsp1286I G_dGCh'C
Cuts at: 349 1081 2195 2308 2919 3012 3086 3276 3338
Size: 732 1114 113 611 93 74 190 62
Cuts at: 3338 4068 349
Size: 730 1575
Fragments arranged by size:
1575 1114 732 730 611 190 113 93
74 62
BspEI T'CCGG_A
Cuts at: 4514 4514
Size: 5294
BspHI T'CATG_A
Cuts at: 1536 4470 1536
Size: 2934 2360
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2344 2558 2860 3241 2344
Size: 214 302 381 4397
Fragments arranged by size:
4397 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3586 3640 5124 5289 50
Size: 3536 54 1484 165 55
Fragments arranged by size:
3536 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3206 3206
Size: 5294
BsrFI r'CCGG_y
Cuts at: 3292 3473 3682 3292
Size: 181 209 4904
Fragments arranged by size:
4904 209 181
BsrGI T'GTAC_A
Cuts at: 279 279
Size: 5294
BssSI C'ACGA_G
Cuts at: 1147 3565 3923 1147
Size: 2418 358 2518
Fragments arranged by size:
2518 2418 358
BstAPI GCAn_nnn'nTGC
Cuts at: 370 1256 1511 1688 1825 2671 370
Size: 886 255 177 137 846 2993
Fragments arranged by size:
2993 886 846 255 177 137
BstBI TT'CG_AA
Cuts at: 3655 3655
Size: 5294
BstXI CCAn_nnnn'nTGG
Cuts at: 244 1003 244
Size: 759 4535
BstYI r'GATC_y
Cuts at: 1 669 1959 2319 2786 2809 3144 3390 4402
Size: 668 1290 360 467 23 335 246 1012
Cuts at: 4402 4488 4500 4846 1
Size: 86 12 346 449
Fragments arranged by size:
1290 1012 668 467 449 360 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1033 1033
Size: 5294
BtgI C'CryG_G
Cuts at: 107 934 1814 107
Size: 827 880 3587
Fragments arranged by size:
3587 880 827
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3244 3425 3588 3244
Size: 181 163 4950
Fragments arranged by size:
4950 181 163
BtsI GCAGTG_nn'
Cuts at: 282 1686 2537 282
Size: 1404 851 3039
Fragments arranged by size:
3039 1404 851
DraI TTT'AAA
Cuts at: 2293 2293
Size: 5294
DrdI GACnn_nn'nnGTC
Cuts at: 3000 3858 3000
Size: 858 4436
EaeI y'GGCC_r
Cuts at: 1409 2879 3053 3444 3471 4816 1409
Size: 1470 174 391 27 1345 1887
Fragments arranged by size:
1887 1470 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2879 2879
Size: 5294
EarI CTCTTCn'nnn_
Cuts at: 1879 2204 3317 3527 1879
Size: 325 1113 210 3646
Fragments arranged by size:
3646 1113 325 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1693 3502 3812 3958 5104 1693
Size: 1809 310 146 1146 1883
Fragments arranged by size:
1883 1809 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2306 2306
Size: 5294
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 341 353 430 479 493 611 1250 3119
Size: 37 12 77 49 14 118 639 1869
Cuts at: 3119 3551 4298 4566 304
Size: 432 747 268 1032
Fragments arranged by size:
1869 1032 747 639 432 268 118 77
49 37 14 12
EcoO109I rG'GnC_Cy
Cuts at: 543 2433 543
Size: 1890 3404
EcoRI G'AATT_C
Cuts at: 2298 2298
Size: 5294
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 562 670 3313 562
Size: 108 2643 2543
Fragments arranged by size:
2643 2543 108
FspI TGC'GCA
Cuts at: 3075 3075
Size: 5294
HaeII r_GCGC'y
Cuts at: 1545 1590 1740 2728 2976 3998 4977 1545
Size: 45 150 988 248 1022 979 1862
Fragments arranged by size:
1862 1022 988 979 248 150 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 3136 3166 3168 3198 3136
Size: 30 2 30 5232
Fragments arranged by size:
5232 30 30 2
HincII GTy'rAC
Cuts at: 270 2333 270
Size: 2063 3231
KasI G'GCGC_C
Cuts at: 2724 2972 2724
Size: 248 5046
KpnI G_GTAC'C
Cuts at: 1370 2314 1370
Size: 944 4350
MfeI C'AATT_G
Cuts at: 2147 2147
Size: 5294
MluI A'CGCG_T
Cuts at: 4727 4727
Size: 5294
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1786 3965 4149 1786
Size: 2179 184 2931
Fragments arranged by size:
2931 2179 184
MscI TGG'CCA
Cuts at: 1411 3055 1411
Size: 1644 3650
MslI CAynn'nnrTG
Cuts at: 906 974 990 1001 1161 1375 1577 1639 3283
Size: 68 16 11 160 214 202 62 1644
Cuts at: 3283 3410 906
Size: 127 2790
Fragments arranged by size:
2790 1644 214 202 160 127 68 62
16 11
MspA1I CmG'CkG
Cuts at: 303 2227 2405 2719 3079 4092 4337 303
Size: 1924 178 314 360 1013 245 1260
Fragments arranged by size:
1924 1260 1013 360 314 245 178
NaeI GCC'GGC
Cuts at: 3475 3475
Size: 5294
NarI GG'CG_CC
Cuts at: 2725 2973 2725
Size: 248 5046
NcoI C'CATG_G
Cuts at: 107 1814 107
Size: 1707 3587
NgoMIV G'CCGG_C
Cuts at: 3473 3473
Size: 5294
NspI r_CATG'y
Cuts at: 1728 1828 2347 2847 3754 1728
Size: 100 519 500 907 3268
Fragments arranged by size:
3268 907 519 500 100
PciI A'CATG_T
Cuts at: 3750 3750
Size: 5294
PfoI T'CCnGG_A
Cuts at: 260 260
Size: 5294
PmeI GTTT'AAAC
Cuts at: 2293 2293
Size: 5294
PmlI CAC'GTG
Cuts at: 929 929
Size: 5294
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1103 1135 4433 4465 1103
Size: 32 3298 32 1932
Fragments arranged by size:
3298 1932 32 32
PsiI TTA'TAA
Cuts at: 394 394
Size: 5294
PstI C_TGCA'G
Cuts at: 1684 2341 3026 1684
Size: 657 685 3952
Fragments arranged by size:
3952 685 657
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5294
PvuII CAG'CTG
Cuts at: 303 2227 2719 3079 303
Size: 1924 492 360 2518
Fragments arranged by size:
2518 1924 492 360
RsrII CG'GwC_CG
Cuts at: 3489 3489
Size: 5294
SacI G_AGCT'C
Cuts at: 2308 2308
Size: 5294
SalI G'TCGA_C
Cuts at: 2331 2331
Size: 5294
SapI GCTCTTCn'nnn_
Cuts at: 3317 3527 3317
Size: 210 5084
SbfI CC_TGCA'GG
Cuts at: 2341 2341
Size: 5294
ScaI AGT'ACT
Cuts at: 4811 4811
Size: 5294
SfcI C'TryA_G
Cuts at: 33 1680 2337 3022 4015 4206 33
Size: 1647 657 685 993 191 1121
Fragments arranged by size:
1647 1121 993 685 657 191
SfoI GGC'GCC
Cuts at: 2726 2974 2726
Size: 248 5046
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5294
SmaI CCC'GGG
Cuts at: 2316 2316
Size: 5294
SmlI C'TyrA_G
Cuts at: 2449 3364 3856 4118 2449
Size: 915 492 262 3625
Fragments arranged by size:
3625 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5294
SphI G_CATG'C
Cuts at: 2347 2847 2347
Size: 500 4794
StyI C'CwwG_G
Cuts at: 107 1814 2428 2782 107
Size: 1707 614 354 2619
Fragments arranged by size:
2619 1707 614 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1159 1453 1669 2877 3010 3469 3678 1159
Size: 294 216 1208 133 459 209 2775
Fragments arranged by size:
2775 1208 459 294 216 209 133
TatI w'GTAC_w
Cuts at: 279 4809 279
Size: 4530 764
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 339 371 4811 4843 339
Size: 32 4440 32 790
Fragments arranged by size:
4440 790 32 32
Tth111I GACn'n_nGTC
Cuts at: 1438 3091 1438
Size: 1653 3641
XbaI T'CTAG_A
Cuts at: 2325 2325
Size: 5294
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 627 1113 2789 627
Size: 486 1676 3132
Fragments arranged by size:
3132 1676 486
XmaI C'CCGG_G
Cuts at: 2314 2314
Size: 5294
XmnI GAAnn'nnTTC
Cuts at: 506 596 901 1118 1156 1308 506
Size: 90 305 217 38 152 4492
Fragments arranged by size:
4492 305 217 152 90 38
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PfoI PmeI PmlI PpiI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI ApaI AscI AvrII
BaeI BcgI BfrBI BmtI BsiWI BssHII BstEII BstZ17I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PpuMI PshAI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI