##################################
# NO CUT ENZYMES (  151 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AocI        Aor51HI     ApaLI       
ApoI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AviII       AvrII       
BamHI       BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BfaI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaI        BsaWI       BseAI       BsiHKAI     
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrDI       BsrGI       
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DraI        DraIII      DrdI        
Eam1105I    EarI        Eco47III    Eco81I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HincII      HindII      HindIII     HinfI       
HpaI        HphI        KpnI        Ksp632I     KspI        
MaeI        MaeII       MaeIII      MamI        MboII       
MfeI        MflI        MluI        MroI        MseI        
MslI        MunI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SfaNI       
SfuI        SgrAI       SmlI        SnaBI       SpeI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TaqI        TfiI        Tru9I       Tsp45I      Tth111I     
TthHB8I     Van91I      XbaI        XhoI        XhoII       
XmnI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

AscI          427  GGCGCGCC             GG^CGCGCC
BalI          371  TGGCCA               TGG^CCA
BpmI          239  CTCCAG               CTGGAG(16/14)
BsmAI          71  GAGAC                GTCTC(1/5)
BspMI         739  ACCTGC               ACCTGC(4/8)
BsrBI         343  GAGCGG               CCGCTC(-3/-3)
BsrFI         228  GCCGGC               [GA]^CCGG[CT]
BsrI          693  CCAGT                ACTGG(1/-1)
Cfr10I        228  GCCGGC               [GA]^CCGG[CT]
DdeI          408  CTCAG                C^T[ACGT]AG
DpnII         330  GATC                 ^GATC
DsaI          374  CCATGG               C^C[GA][CT]GG
MboI          330  GATC                 ^GATC
MluNI         371  TGGCCA               TGG^CCA
MscI          371  TGGCCA               TGG^CCA
NaeI          228  GCCGGC               GCC^GGC
NcoI          374  CCATGG               C^CATGG
NdeII         330  GATC                 ^GATC
NgoMIV        228  GCCGGC               G^CCGGC
NspI          353  GCATGC               [GA]CATG^[CT]
PstI          505  CTGCAG               CTGCA^G
PvuII         316  CAGCTG               CAG^CTG
Sau3AI        330  GATC                 ^GATC
SexAI         764  ACCTGGT              A^CC[AT]GGT
SfiI           56  GGCCACAGCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI          353  GCATGC               GCATG^C
Tsp509I       806  AATT                 ^AATT
TspRI         418  CACTG                CA[GC]TG[ACGT][ACGT]^
XcmI          776  CCAGCCTGGGCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AfaI            3  GTAC                 GT^AC
              657  GTAC                
ApaI          250  GGGCCC               GGGCC^C
              541  GGGCCC              
BanII         250  GGGCCC               G[GA]GC[CT]^C
              541  GGGCCC              
BglI           57  GCCACAGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              793  GCCGTGCAGGC         
BmyI          250  GGGCCC               G[AGT]GC[ACT]^C
              541  GGGCCC              
BsgI          735  CTGCAC               GTGCAG(16/14)
              796  GTGCAG              
Bsp1286I      250  GGGCCC               G[AGT]GC[ACT]^C
              541  GGGCCC              
BstAPI        312  GCAGCAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              507  GCAGGAGCTGC         
BstF5I        396  CATCC                GGATG(2/0)
              483  CATCC               
EcoNI         195  CCTCCCCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              384  CCTGCGCGAGG         
FokI          396  CATCC                GGATG(9/13)
              483  CATCC               
PpuMI         359  AGGACCT              [GA]G^G[AT]CC[CT]
              627  AGGACCC             
RsaI            3  GTAC                 GT^AC
              657  GTAC                
SfcI          111  CTACAG               C^T[GA][CT]AG
              505  CTGCAG              


#########################################
# 3 OR MORE CUT ENZYMES (   64 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AlwNI       
AvaI        AvaII       BanI        BbeI        BbvI        
BcnI        BsaHI       BsaJI       BseRI       BsiEI       
BsiYI       BslI        BsmFI       BsoBI       BssHII      
BssKI       BstNI       BstUI       Cac8I       CfoI        
Cfr13I      DraII       EaeI        EagI        EclXI       
Eco52I      EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HpaII       ItaI        
KasI        MnlI        MspA1I      MspI        MvaI        
MvnI        MwoI        NarI        NciI        NlaIII      
NlaIV       Sau96I      ScrFI       SfoI        SmaI        
StyI        TseI        XmaCI       XmaI