Restriction map for pF1KB5281
 (Linear) (Six-base) MAPSORT of: KIBB5281.seq  Check: 8372  from: 1  to: 816



KIBB5281.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:37  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     748     816 
   Size:        748      68
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     520     816 
   Size:        520     296
 
AlwNI CAG_nnn'CTG

Cuts at:      0     318     363     513     816 
   Size:        318      45     150     303

  Fragments arranged by size: 

                 318     303     150      45
 
ApaI G_GGCC'C

Cuts at:      0     254     545     816 
   Size:        254     291     271

  Fragments arranged by size: 

                 291     271     254
 
AscI GG'CGCG_CC

Cuts at:      0     428     816 
   Size:        428     388
 
AvaI C'yCGr_G

Cuts at:      0      40      82     247     253     270     816 
   Size:         40      42     165       6      17     546

  Fragments arranged by size: 

                 546     165      42      40      17       6
 
BanI G'GyrC_C

Cuts at:      0     225     535     612     619     816 
   Size:        225     310      77       7     197

  Fragments arranged by size: 

                 310     225     197      77       7
 
BanII G_rGCy'C

Cuts at:      0     254     545     816 
   Size:        254     291     271

  Fragments arranged by size: 

                 291     271     254
 
BbeI G_GCGC'C

Cuts at:      0     229     539     616     623     816 
   Size:        229     310      77       7     193

  Fragments arranged by size: 

                 310     229     193      77       7
 
BglI GCCn_nnn'nGGC

Cuts at:      0      63     799     816 
   Size:         63     736      17

  Fragments arranged by size: 

                 736      63      17
 
Bme1580I G_kGCm'C

Cuts at:      0     254     545     816 
   Size:        254     291     271

  Fragments arranged by size: 

                 291     271     254
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     224     816 
   Size:        224     592
 
BsaHI Gr'CG_yC

Cuts at:      0     226     293     380     536     613     620     816 
   Size:        226      67      87     156      77       7     196

  Fragments arranged by size: 

                 226     196     156      87      77      67       7
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     117     120     123     126     129     132     141     150 
   Size:        117       3       3       3       3       3       9       9

Cuts at:    150     162     165     184     316     816 
   Size:         12       3      19     132     500

  Fragments arranged by size: 

                 500     132     117      19      12       9       9       3
                   3       3       3       3       3
 
BseYI C'CCAG_C

Cuts at:      0     492     525     816 
   Size:        492      33     291

  Fragments arranged by size: 

                 492     291      33
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     720     816 
   Size:        720      96
 
BsiEI CG_ry'CG

Cuts at:      0     233     289     438     816 
   Size:        233      56     149     378

  Fragments arranged by size: 

                 378     233     149      56
 
Bsp1286I G_dGCh'C

Cuts at:      0     254     545     816 
   Size:        254     291     271

  Fragments arranged by size: 

                 291     271     254
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     748     816 
   Size:        748      68
 
BsrBI CCG'CTC

Cuts at:      0     345     816 
   Size:        345     471
 
BsrFI r'CCGG_y

Cuts at:      0     228     816 
   Size:        228     588
 
BssHII G'CGCG_C

Cuts at:      0      22     208     263     428     521     816 
   Size:         22     186      55     165      93     295

  Fragments arranged by size: 

                 295     186     165      93      55      22
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     318     513     816 
   Size:        318     195     303

  Fragments arranged by size: 

                 318     303     195
 
BtgI C'CryG_G

Cuts at:      0     374     816 
   Size:        374     442
 
BtsI GCAGTG_nn'

Cuts at:      0     417     816 
   Size:        417     399
 
EaeI y'GGCC_r

Cuts at:      0      55     230     286     371     435     566     690     773 
   Size:         55     175      56      85      64     131     124      83

Cuts at:    773     788     816 
   Size:         15      28

  Fragments arranged by size: 

                 175     131     124      85      83      64      56      55
                  28      15
 
EagI C'GGCC_G

Cuts at:      0     230     286     435     816 
   Size:        230      56     149     381

  Fragments arranged by size: 

                 381     230     149      56
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     224     816 
   Size:        224     592
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     199     388     816 
   Size:        199     189     428

  Fragments arranged by size: 

                 428     199     189
 
EcoO109I rG'GnC_Cy

Cuts at:      0      86     244     360     628     816 
   Size:         86     158     116     268     188

  Fragments arranged by size: 

                 268     188     158     116      86
 
HaeII r_GCGC'y

Cuts at:      0     229     284     539     616     623     673     816 
   Size:        229      55     255      77       7      50     143

  Fragments arranged by size: 

                 255     229     143      77      55      50       7
 
KasI G'GCGC_C

Cuts at:      0     225     535     612     619     816 
   Size:        225     310      77       7     197

  Fragments arranged by size: 

                 310     225     197      77       7
 
MscI TGG'CCA

Cuts at:      0     373     816 
   Size:        373     443
 
MspA1I CmG'CkG

Cuts at:      0      63     318     412     816 
   Size:         63     255      94     404

  Fragments arranged by size: 

                 404     255      94      63
 
NaeI GCC'GGC

Cuts at:      0     230     816 
   Size:        230     586
 
NarI GG'CG_CC

Cuts at:      0     226     536     613     620     816 
   Size:        226     310      77       7     196

  Fragments arranged by size: 

                 310     226     196      77       7
 
NcoI C'CATG_G

Cuts at:      0     374     816 
   Size:        374     442
 
NgoMIV G'CCGG_C

Cuts at:      0     228     816 
   Size:        228     588
 
NspI r_CATG'y

Cuts at:      0     357     816 
   Size:        357     459
 
PpuMI rG'GwC_Cy

Cuts at:      0     360     628     816 
   Size:        360     268     188

  Fragments arranged by size: 

                 360     268     188
 
PspOMI G'GGCC_C

Cuts at:      0     250     541     816 
   Size:        250     291     275

  Fragments arranged by size: 

                 291     275     250
 
PstI C_TGCA'G

Cuts at:      0     509     816 
   Size:        509     307
 
PvuII CAG'CTG

Cuts at:      0     318     816 
   Size:        318     498
 
SexAI A'CCwGG_T

Cuts at:      0     764     816 
   Size:        764      52
 
SfcI C'TryA_G

Cuts at:      0     111     505     816 
   Size:        111     394     311

  Fragments arranged by size: 

                 394     311     111
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      63     816 
   Size:         63     753
 
SfoI GGC'GCC

Cuts at:      0     227     537     614     621     816 
   Size:        227     310      77       7     195

  Fragments arranged by size: 

                 310     227     195      77       7
 
SmaI CCC'GGG

Cuts at:      0      42     249     255     272     816 
   Size:         42     207       6      17     544

  Fragments arranged by size: 

                 544     207      42      17       6
 
SphI G_CATG'C

Cuts at:      0     357     816 
   Size:        357     459
 
StyI C'CwwG_G

Cuts at:      0     200     296     374     816 
   Size:        200      96      78     442

  Fragments arranged by size: 

                 442     200      96      78
 
TatI w'GTAC_w

Cuts at:      0       2     816 
   Size:          2     814
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     783     816 
   Size:        783      33
 
XmaI C'CCGG_G

Cuts at:      0      40     247     253     270     816 
   Size:         40     207       6      17     546

  Fragments arranged by size: 

                 546     207      40      17       6
 

 Enzymes that do cut:

     AarI     AlfI    AlwNI     ApaI     AscI     AvaI     BanI    BanII
     BbeI     BglI Bme1580I     BpmI    BsaHI    BseRI    BseYI     BsgI
    BsiEI Bsp1286I    BspMI    BsrBI    BsrFI   BssHII   BstAPI     BtgI
     BtsI     EaeI     EagI  Eco57MI    EcoNI EcoO109I    HaeII     KasI
     MscI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI    PpuMI
   PspOMI     PstI    PvuII    SexAI     SfcI     SfiI     SfoI     SmaI
     SphI     StyI     TatI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    ApaLI     ApoI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrDI    BsrGI    BssSI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EarI     EciI  EcoICRI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     MslI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI     SgfI
    SgrAI     SmlI    SnaBI     SpeI     SrfI     SspI     StuI     SwaI
    TaqII     TstI  Tth111I     XbaI     XhoI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com