Restriction map for pF1KB5144
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# NO CUT ENZYMES (  131 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        AhdI        
Alw44I      Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BanI        
BbeI        BbrPI       BbvCI       BcnI        BfrI        
BglI        BglII       BlnI        BsaAI       BsaHI       
BsaI        BsaWI       BseAI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsoBI       Bsp1407I    BspDI       
BspEI       BspMI       BsrBI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstUI       BstXI       BstZ17I     Cac8I       CfoI        
ClaI        CpoI        DraIII      DrdI        DsaI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoNI       EcoO65I     EcoRV       FseI        FspI        
HaeII       HhaI        Hin1I       HinP1I      HincII      
HindII      HpaI        KasI        KpnI        KspI        
MluI        MroI        MvnI        NaeI        NarI        
NciI        NcoI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacI        
SacII       SalI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SwaI        Tth111I     XbaI        XhoI        XmaCI       
XmaI        


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# ONE CUT ENZYMES (   58 enzymes) #
###################################

AflIII        851  ACATGT               A^C[GA][CT]GT
AocI          926  CCTTAGG              CC^T[ACGT]AGG
BalI          126  TGGCCA               TGG^CCA
BamHI        2098  GGATCC               G^GATCC
BanII         883  GAGCCC               G[GA]GC[CT]^C
BcgI         2045  CGAGCGAGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        2112  GGATAC               GTATCC(6/5)
BclI          728  TGATCA               T^GATCA
BlpI         1319  GCTTAGC              GC^T[ACGT]AGC
BmyI          883  GAGCCC               G[AGT]GC[ACT]^C
Bpu1102I     1319  GCTTAGC              GC^T[ACGT]AGC
BsaBI         681  GATGACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI        1730  CTCCTC               GAGGAG(10/8)
BsmBI        1117  GAGACG               CGTCTC(1/5)
Bsp1286I      883  GAGCCC               G[AGT]GC[ACT]^C
BspHI        1124  TCATGA               T^CATGA
BspLU11I      851  ACATGT               A^CATGT
BsrFI        1315  ACCGGC               [GA]^CCGG[CT]
BstAPI        522  GCAACCCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I        210  CATCC                GGATG(2/0)
Bsu36I        926  CCTTAGG              CC^T[ACGT]AGG
CelII        1319  GCTTAGC              GC^T[ACGT]AGC
Cfr10I       1315  ACCGGC               [GA]^CCGG[CT]
DraI          822  TTTAAA               TTT^AAA
DraII        1982  GGGTCCT              [GA]G^G[ACGT]CC[CT]
EaeI          126  TGGCCA               [CT]^GGCC[GA]
Eco81I        926  CCTTAGG              CC^T[ACGT]AGG
EcoO109I     1982  GGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoRI         371  GAATTC               G^AATTC
EcoT22I      1098  ATGCAT               ATGCA^T
FauI          422  GCGGG                CCCGC(4/6)
FbaI          728  TGATCA               T^GATCA
FokI          210  CATCC                GGATG(9/13)
HapII        1316  CCGG                 C^CGG
HgaI           82  GACGC                GACGC(5/10)
HindIII       865  AAGCTT               A^AGCTT
HpaII        1316  CCGG                 C^CGG
HphI          552  GGTGA                GGTGA(8/7)
MaeII         476  ACGT                 A^CGT
MamI          681  GATGACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          833  CAATTG               C^AATTG
MluNI         126  TGGCCA               TGG^CCA
MscI          126  TGGCCA               TGG^CCA
MspA1I         86  CAGCTG               C[AC]G^C[GT]G
MspI         1316  CCGG                 C^CGG
MunI          833  CAATTG               C^AATTG
NdeI          782  CATATG               CA^TATG
NsiI         1098  ATGCAT               ATGCA^T
NspI          851  ACATGT               [GA]CATG^[CT]
PleI           52  GAGTC                GAGTC(4/5)
PpuMI        1982  GGGTCCT              [GA]G^G[AT]CC[CT]
PstI         1196  CTGCAG               CTGCA^G
PvuII          86  CAGCTG               CAG^CTG
RcaI         1124  TCATGA               T^CATGA
SmlI          911  CTCAAG               C^T[CT][GA]AG
StuI         1410  AGGCCT               AGG^CCT
TfiI         2179  GAATC                G^A[AT]TC
XcmI          686  CCATCTGCTTGTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AciI          287  GCGG                 CCGC(-3/-1)
              422  GCGG                
AlwNI        1895  CAGTCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2177  CAGAATCTG           
Asp700I      1246  GAAGAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1825  GAAGAATTTC          
AvaII        1313  GGACC                G^G[AT]CC
             1983  GGTCC               
BfaI         1408  CTAG                 C^TAG
             1760  CTAG                
BsrDI         951  CATTGC               GCAATG(2/0)
             1748  CATTGC              
BstYI         160  GGATCT               [GA]^GATC[CT]
             2098  GGATCC              
HinfI          52  GAGTC                G^A[ACGT]TC
             2179  GAATC               
MaeI         1408  CTAG                 C^TAG
             1760  CTAG                
MflI          160  GGATCT               [GA]^GATC[CT]
             2098  GGATCC              
MslI          263  CATACCTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              991  CACTTTGATG          
PflMI         887  CCAAGGTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2062  CCAAATTTTGG         
SapI         1288  GAAGAGC              GCTCTTC(1/4)
             1258  GCTCTTC             
TaqI          170  TCGA                 T^CGA
             2085  TCGA                
Tsp45I       1447  GTCAC                ^GT[GC]AC
             1963  GTGAC               
TthHB8I       170  TCGA                 T^CGA
             2085  TCGA                
Van91I        887  CCAAGGTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2062  CCAAATTTTGG         
XhoII         160  GGATCT               [GA]^GATC[CT]
             2098  GGATCC              
XmnI         1246  GAAGAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1825  GAAGAATTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   50 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        ApoI        
BbsI        BbvI        BpmI        BpuAI       BsaJI       
BsiYI       BslI        BsmAI       BsmFI       BsmI        
BsrI        BssKI       BstNI       Cfr13I      DdeI        
DpnII       EarI        EcoRII      EcoT14I     Fnu4HI      
HaeIII      ItaI        Ksp632I     MaeIII      MboI        
MboII       MnlI        MseI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SspI        
StyI        Tru9I       TseI        Tsp509I     TspRI       
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