Restriction map for pF1KB5144
 (Linear) (Six-base) MAPSORT of: KIBB5144.seq  Check: 9562  from: 1  to: 2190



KIBB5144.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:37  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     185    1844    2190 
   Size:        185    1659     346

  Fragments arranged by size: 

                1659     346     185
 
AflIII A'CryG_T

Cuts at:      0     851    2190 
   Size:        851    1339
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1900    2182    2190 
   Size:       1900     282       8

  Fragments arranged by size: 

                1900     282       8
 
ApoI r'AATT_y

Cuts at:      0     371    1110    1129    1484    1828    2064    2190 
   Size:        371     739      19     355     344     236     126

  Fragments arranged by size: 

                 739     371     355     344     236     126      19
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     134     167    2190 
   Size:        134      33    2023

  Fragments arranged by size: 

                2023     134      33
 
BamHI G'GATC_C

Cuts at:      0    2098    2190 
   Size:       2098      92
 
BanII G_rGCy'C

Cuts at:      0     887    2190 
   Size:        887    1303
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      86     737    1498    1670    2190 
   Size:         86     651     761     172     520

  Fragments arranged by size: 

                 761     651     520     172      86
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2034    2068    2190 
   Size:       2034      34     122

  Fragments arranged by size: 

                2034     122      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2106    2190 
   Size:       2106      84
 
BclI T'GATC_A

Cuts at:      0     728    2190 
   Size:        728    1462
 
BfrBI ATG'CAT

Cuts at:      0    1100    2190 
   Size:       1100    1090
 
BlpI GC'TnA_GC

Cuts at:      0    1320    2190 
   Size:       1320     870
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     427    1888    2190 
   Size:        427    1461     302

  Fragments arranged by size: 

                1461     427     302
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     510     901     915    2190 
   Size:        510     391      14    1275

  Fragments arranged by size: 

                1275     510     391      14
 
Bpu10I CC'TnA_GC

Cuts at:      0     863    2190 
   Size:        863    1327
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     896    2190 
   Size:        896    1294
 
BsaBI GATnn'nnATC

Cuts at:      0     685    2190 
   Size:        685    1505
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1721    2190 
   Size:       1721     469
 
BseYI C'CCAG_C

Cuts at:      0    1680    1693    2190 
   Size:       1680      13     497

  Fragments arranged by size: 

                1680     497      13
 
BsmI GAATG_Cn'

Cuts at:      0     737     814     842    2190 
   Size:        737      77      28    1348

  Fragments arranged by size: 

                1348     737      77      28
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1111    2190 
   Size:       1111    1079
 
Bsp1286I G_dGCh'C

Cuts at:      0     887    2190 
   Size:        887    1303
 
BspHI T'CATG_A

Cuts at:      0    1124    2190 
   Size:       1124    1066
 
BsrDI GCAATG_nn'

Cuts at:      0     950    1747    2190 
   Size:        950     797     443

  Fragments arranged by size: 

                 950     797     443
 
BsrFI r'CCGG_y

Cuts at:      0    1315    2190 
   Size:       1315     875
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     528    2190 
   Size:        528    1662
 
BstYI r'GATC_y

Cuts at:      0     160    2098    2190 
   Size:        160    1938      92

  Fragments arranged by size: 

                1938     160      92
 
Bsu36I CC'TnA_GG

Cuts at:      0     927    2190 
   Size:        927    1263
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1360    2027    2190 
   Size:       1360     667     163

  Fragments arranged by size: 

                1360     667     163
 
BtsI GCAGTG_nn'

Cuts at:      0    1588    2190 
   Size:       1588     602
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1031    1066    2190 
   Size:       1031      35    1124

  Fragments arranged by size: 

                1124    1031      35
 
DraI TTT'AAA

Cuts at:      0     824    2190 
   Size:        824    1366
 
EaeI y'GGCC_r

Cuts at:      0     126    2190 
   Size:        126    2064
 
EarI CTCTTCn'nnn_

Cuts at:      0     128    1241    1265    1283    2190 
   Size:        128    1113      24      18     907

  Fragments arranged by size: 

                1113     907     128      24      18
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     185     510     901     915    1844    2190 
   Size:        185     325     391      14     929     346

  Fragments arranged by size: 

                 929     391     346     325     185      14
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1983    2190 
   Size:       1983     207
 
EcoRI G'AATT_C

Cuts at:      0     371    2190 
   Size:        371    1819
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      85     203    1504    2190 
   Size:         85     118    1301     686

  Fragments arranged by size: 

                1301     686     118      85
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     152     184    1804    1836    1967    1999    2041    2043 
   Size:        152      32    1620      32     131      32      42       2

Cuts at:   2043    2073    2075    2190 
   Size:         30       2     115

  Fragments arranged by size: 

                1620     152     131     115      42      32      32      32
                  30       2       2
 
HindIII A'AGCT_T

Cuts at:      0     865    2190 
   Size:        865    1325
 
MfeI C'AATT_G

Cuts at:      0     833    2190 
   Size:        833    1357
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     204    1867    2190 
   Size:        204    1663     323

  Fragments arranged by size: 

                1663     323     204
 
MscI TGG'CCA

Cuts at:      0     128    2190 
   Size:        128    2062
 
MslI CAynn'nnrTG

Cuts at:      0     267     995    2190 
   Size:        267     728    1195

  Fragments arranged by size: 

                1195     728     267
 
MspA1I CmG'CkG

Cuts at:      0      88    2190 
   Size:         88    2102
 
NdeI CA'TA_TG

Cuts at:      0     783    2190 
   Size:        783    1407
 
NsiI A_TGCA'T

Cuts at:      0    1102    2190 
   Size:       1102    1088
 
NspI r_CATG'y

Cuts at:      0     855    2190 
   Size:        855    1335
 
PciI A'CATG_T

Cuts at:      0     851    2190 
   Size:        851    1339
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     893    2068    2190 
   Size:        893    1175     122

  Fragments arranged by size: 

                1175     893     122
 
PpuMI rG'GwC_Cy

Cuts at:      0    1983    2190 
   Size:       1983     207
 
PstI C_TGCA'G

Cuts at:      0    1200    2190 
   Size:       1200     990
 
PvuII CAG'CTG

Cuts at:      0      88    2190 
   Size:         88    2102
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1265    1283    2190 
   Size:       1265      18     907

  Fragments arranged by size: 

                1265     907      18
 
SfcI C'TryA_G

Cuts at:      0     233    1196    1901    2190 
   Size:        233     963     705     289

  Fragments arranged by size: 

                 963     705     289     233
 
SmlI C'TyrA_G

Cuts at:      0     911    2190 
   Size:        911    1279
 
SspI AAT'ATT

Cuts at:      0     639    1623    2079    2190 
   Size:        639     984     456     111

  Fragments arranged by size: 

                 984     639     456     111
 
StuI AGG'CCT

Cuts at:      0    1412    2190 
   Size:       1412     778
 
StyI C'CwwG_G

Cuts at:      0     532     887    1413    2190 
   Size:        532     355     526     777

  Fragments arranged by size: 

                 777     532     526     355
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     693    2190 
   Size:        693    1497
 
XmnI GAAnn'nnTTC

Cuts at:      0    1250    1829    2190 
   Size:       1250     579     361

  Fragments arranged by size: 

                1250     579     361
 

 Enzymes that do cut:

     AcuI   AflIII    AlwNI     ApoI     BaeI    BamHI    BanII     BbsI
     BcgI    BciVI     BclI    BfrBI     BlpI     BmrI     BpmI   Bpu10I
    BpuEI    BsaBI    BseRI    BseYI     BsmI    BsmBI Bsp1286I    BspHI
    BsrDI    BsrFI   BstAPI    BstYI   Bsu36I    BtgZI     BtsI    CspCI
     DraI     EaeI     EarI  Eco57MI EcoO109I    EcoRI     FalI    Hin4I
  HindIII     MfeI     MmeI     MscI     MslI   MspA1I     NdeI     NsiI
     NspI     PciI    PflMI    PpuMI     PstI    PvuII     SapI     SfcI
     SmlI     SspI     StuI     StyI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BanI     BbeI    BbvCI     BglI    BglII
 Bme1580I    BmgBI     BmtI     BplI     BsaI    BsaAI    BsaHI    BsaWI
    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI    BspEI    BspMI    BsrBI
    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I     BtgI
     ClaI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRV
     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI     KpnI
     MluI     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI
     PacI     PasI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XhoI     XmaI     ZraI
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