Restriction map for pF1KB5121
 (Linear) (Six-base) MAPSORT of: KIBB5121.seq  Check: 1298  from: 1  to: 1422



KIBB5121.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:37  ..

AccI GT'mk_AC

Cuts at:      0     182    1082    1422 
   Size:        182     900     340

  Fragments arranged by size: 

                 900     340     182
 
Acc65I G'GTAC_C

Cuts at:      0     351    1266    1422 
   Size:        351     915     156

  Fragments arranged by size: 

                 915     351     156
 
AflIII A'CryG_T

Cuts at:      0      98    1422 
   Size:         98    1324
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1353    1422 
   Size:       1353      69
 
AleI CACnn'nnGTG

Cuts at:      0     844    1156    1422 
   Size:        844     312     266

  Fragments arranged by size: 

                 844     312     266
 
AlwNI CAG_nnn'CTG

Cuts at:      0     601    1422 
   Size:        601     821
 
ApaI G_GGCC'C

Cuts at:      0     176    1422 
   Size:        176    1246
 
ApaLI G'TGCA_C

Cuts at:      0      71    1422 
   Size:         71    1351
 
ApoI r'AATT_y

Cuts at:      0     568    1338    1422 
   Size:        568     770      84

  Fragments arranged by size: 

                 770     568      84
 
AscI GG'CGCG_CC

Cuts at:      0    1245    1422 
   Size:       1245     177
 
BanI G'GyrC_C

Cuts at:      0     351    1266    1422 
   Size:        351     915     156

  Fragments arranged by size: 

                 915     351     156
 
BanII G_rGCy'C

Cuts at:      0     176    1422 
   Size:        176    1246
 
BbvCI CC'TCA_GC

Cuts at:      0    1105    1422 
   Size:       1105     317
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     698    1422 
   Size:        698     724
 
BfrBI ATG'CAT

Cuts at:      0     811    1422 
   Size:        811     611
 
Bme1580I G_kGCm'C

Cuts at:      0      75     176    1422 
   Size:         75     101    1246

  Fragments arranged by size: 

                1246     101      75
 
BmtI G_CTAG'C

Cuts at:      0    1025    1422 
   Size:       1025     397
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     550     985    1081    1422 
   Size:        550     435      96     341

  Fragments arranged by size: 

                 550     435     341      96
 
Bpu10I CC'TnA_GC

Cuts at:      0    1105    1222    1422 
   Size:       1105     117     200

  Fragments arranged by size: 

                1105     200     117
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     228     877    1422 
   Size:        228     649     545

  Fragments arranged by size: 

                 649     545     228
 
BsaAI yAC'GTr

Cuts at:      0     108    1422 
   Size:        108    1314
 
BsaBI GATnn'nnATC

Cuts at:      0     196     923    1422 
   Size:        196     727     499

  Fragments arranged by size: 

                 727     499     196
 
BsaHI Gr'CG_yC

Cuts at:      0    1085    1422 
   Size:       1085     337
 
BsaWI w'CCGG_w

Cuts at:      0    1121    1422 
   Size:       1121     301
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     208     238    1422 
   Size:        208      30    1184

  Fragments arranged by size: 

                1184     208      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     599    1093    1422 
   Size:        599     494     329

  Fragments arranged by size: 

                 599     494     329
 
BseYI C'CCAG_C

Cuts at:      0      91    1270    1301    1422 
   Size:         91    1179      31     121

  Fragments arranged by size: 

                1179     121      91      31
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     507    1422 
   Size:        507     915
 
BsiEI CG_ry'CG

Cuts at:      0     160    1422 
   Size:        160    1262
 
BsiHKAI G_wGCw'C

Cuts at:      0      75    1151    1422 
   Size:         75    1076     271

  Fragments arranged by size: 

                1076     271      75
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1123    1422 
   Size:       1123     299
 
Bsp1286I G_dGCh'C

Cuts at:      0      75     176    1151    1422 
   Size:         75     101     975     271

  Fragments arranged by size: 

                 975     271     101      75
 
BspEI T'CCGG_A

Cuts at:      0    1121    1422 
   Size:       1121     301
 
BsrFI r'CCGG_y

Cuts at:      0    1256    1422 
   Size:       1256     166
 
BsrGI T'GTAC_A

Cuts at:      0    1416    1422 
   Size:       1416       6
 
BssHII G'CGCG_C

Cuts at:      0    1245    1422 
   Size:       1245     177
 
BssSI C'ACGA_G

Cuts at:      0    1152    1422 
   Size:       1152     270
 
BstBI TT'CG_AA

Cuts at:      0     495    1422 
   Size:        495     927
 
BstYI r'GATC_y

Cuts at:      0     489     896    1316    1422 
   Size:        489     407     420     106

  Fragments arranged by size: 

                 489     420     407     106
 
Bsu36I CC'TnA_GG

Cuts at:      0     877    1422 
   Size:        877     545
 
BtgI C'CryG_G

Cuts at:      0      59    1089    1422 
   Size:         59    1030     333

  Fragments arranged by size: 

                1030     333      59
 
ClaI AT'CG_AT

Cuts at:      0     200    1422 
   Size:        200    1222
 
EaeI y'GGCC_r

Cuts at:      0     157    1422 
   Size:        157    1265
 
EagI C'GGCC_G

Cuts at:      0     157    1422 
   Size:        157    1265
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     550     985    1081    1422 
   Size:        550     435      96     341

  Fragments arranged by size: 

                 550     435     341      96
 
EcoO109I rG'GnC_Cy

Cuts at:      0     172     173    1003    1422 
   Size:        172       1     830     419

  Fragments arranged by size: 

                 830     419     172       1
 
EcoRI G'AATT_C

Cuts at:      0    1338    1422 
   Size:       1338      84
 
EcoRV GAT'ATC

Cuts at:      0     502    1422 
   Size:        502     920
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     308    1422 
   Size:        308    1114
 
HaeII r_GCGC'y

Cuts at:      0    1199    1422 
   Size:       1199     223
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     208     240     367     399     446     478    1339    1371 
   Size:        208      32     127      32      47      32     861      32

Cuts at:   1371    1422 
   Size:         51

  Fragments arranged by size: 

                 861     208     127      51      47      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     183     642    1083    1422 
   Size:        183     459     441     339

  Fragments arranged by size: 

                 459     441     339     183
 
KpnI G_GTAC'C

Cuts at:      0     355    1270    1422 
   Size:        355     915     152

  Fragments arranged by size: 

                 915     355     152
 
MfeI C'AATT_G

Cuts at:      0     635    1422 
   Size:        635     787
 
MslI CAynn'nnrTG

Cuts at:      0     217     434     844    1156    1422 
   Size:        217     217     410     312     266

  Fragments arranged by size: 

                 410     312     266     217     217
 
MspA1I CmG'CkG

Cuts at:      0     475    1422 
   Size:        475     947
 
NcoI C'CATG_G

Cuts at:      0      59    1422 
   Size:         59    1363
 
NheI G'CTAG_C

Cuts at:      0    1021    1422 
   Size:       1021     401
 
NsiI A_TGCA'T

Cuts at:      0     813    1422 
   Size:        813     609
 
NspI r_CATG'y

Cuts at:      0     102     482     756    1422 
   Size:        102     380     274     666

  Fragments arranged by size: 

                 666     380     274     102
 
PciI A'CATG_T

Cuts at:      0      98    1422 
   Size:         98    1324
 
PspOMI G'GGCC_C

Cuts at:      0     172    1422 
   Size:        172    1250
 
PstI C_TGCA'G

Cuts at:      0     819    1422 
   Size:        819     603
 
PvuII CAG'CTG

Cuts at:      0     475    1422 
   Size:        475     947
 
SalI G'TCGA_C

Cuts at:      0     181    1081    1422 
   Size:        181     900     341

  Fragments arranged by size: 

                 900     341     181
 
SfcI C'TryA_G

Cuts at:      0     815    1422 
   Size:        815     607
 
StuI AGG'CCT

Cuts at:      0    1237    1422 
   Size:       1237     185
 
StyI C'CwwG_G

Cuts at:      0      59    1169    1422 
   Size:         59    1110     253

  Fragments arranged by size: 

                1110     253      59
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     774     886    1422 
   Size:        774     112     536

  Fragments arranged by size: 

                 774     536     112
 
TatI w'GTAC_w

Cuts at:      0       9    1181    1416    1422 
   Size:          9    1172     235       6

  Fragments arranged by size: 

                1172     235       9       6
 
Tth111I GACn'n_nGTC

Cuts at:      0     220    1422 
   Size:        220    1202
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     216    1422 
   Size:        216    1206
 

 Enzymes that do cut:

     AccI   Acc65I   AflIII     AhdI     AleI    AlwNI     ApaI    ApaLI
     ApoI     AscI     BanI    BanII    BbvCI    BciVI    BfrBI Bme1580I
     BmtI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I
    BspEI    BsrFI    BsrGI   BssHII    BssSI    BstBI    BstYI   Bsu36I
     BtgI     ClaI     EaeI     EagI  Eco57MI EcoO109I    EcoRI    EcoRV
     FalI    HaeII    Hin4I   HincII     KpnI     MfeI     MslI   MspA1I
     NcoI     NheI     NsiI     NspI     PciI   PspOMI     PstI    PvuII
     SalI     SfcI     StuI     StyI    TaqII     TatI  Tth111I     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AcuI     AfeI    AflII     AgeI     AlfI
     AloI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI
     BbsI     BcgI     BclI     BglI    BglII     BlpI    BmgBI     BmrI
     BplI    BpuEI    BsiWI     BsmI    BspHI    BspMI    BsrBI    BsrDI
   BstAPI   BstEII    BstXI  BstZ17I    BtgZI     BtsI    CspCI     DraI
   DraIII     DrdI     EarI     EciI  EcoICRI    EcoNI     FseI     FspI
    FspAI  HindIII     HpaI     KasI     MluI     MmeI     MscI     NaeI
     NarI     NdeI   NgoMIV     NotI     NruI     PacI     PasI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TstI     XbaI
     XhoI     XmaI     XmnI     ZraI
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