Restriction map for pF1KB5115
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# NO CUT ENZYMES (  124 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AgeI        Alw44I      AocI        
Aor51HI     ApaLI       AscI        AseI        AsnI        
Asp718I     AspI        AvaI        AviII       AvrII       
BamHI       BbeI        BbrPI       BciVI       BcnI        
BfrI        BglI        BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1407I    BspEI       BspHI       BsrDI       
BsrGI       BssHII      BssSI       BstBI       BstEII      
BstPI       BstUI       Bsu36I      CelII       CfoI        
CpoI        DrdI        EagI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FseI        FspI        HaeII       
HhaI        Hin1I       HinP1I      HindIII     HpaI        
KasI        KpnI        KspI        MamI        MluI        
MroI        MvnI        NaeI        NarI        NciI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SalI        SbfI        ScaI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    SspBI       StuI        Tth111I     
XbaI        XhoI        XmaCI       XmaI        


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# ONE CUT ENZYMES (   40 enzymes) #
###################################

AhdI         3061  GACAACATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I      1520  GAAGCAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        3061  GACAACATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI            2  TGGCCA               TGG^CCA
BcgI         3096  GCATGGGGCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII         414  AGATCT               A^GATCT
BsaI         2633  GAGACC               GGTCTC(1/5)
BspDI         677  ATCGAT               AT^CGAT
BsrBI         437  GAGCGG               CCGCTC(-3/-3)
BsrFI         231  GCCGGT               [GA]^CCGG[CT]
Bst1107I      823  GTATAC               GTA^TAC
BstAPI        379  GCATCAGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I       823  GTATAC               GTA^TAC
Cfr10I        231  GCCGGT               [GA]^CCGG[CT]
ClaI          677  ATCGAT               AT^CGAT
DraIII       1091  CACACTGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I     3061  GACAACATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FauI         1043  CCCGC                CCCGC(4/6)
HapII         232  CCGG                 C^CGG
HgaI          350  GCGTC                GACGC(5/10)
HincII       1573  GTTGAC               GT[CT]^[GA]AC
HindII       1573  GTTGAC               GT[CT]^[GA]AC
HpaII         232  CCGG                 C^CGG
MaeII        2943  ACGT                 A^CGT
MfeI         1167  CAATTG               C^AATTG
MluNI           2  TGGCCA               TGG^CCA
MscI            2  TGGCCA               TGG^CCA
MspI          232  CCGG                 C^CGG
MunI         1167  CAATTG               C^AATTG
NdeI          964  CATATG               CA^TATG
PflMI           9  CCAGGTAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          614  CTGCAG               CTGCA^G
PvuII         180  CAGCTG               CAG^CTG
SacI          456  GAGCTC               GAGCT^C
SapI          605  GCTCTTC              GCTCTTC(1/4)
SphI         1905  GCATGC               GCATG^C
SspI         1318  AATATT               AAT^ATT
SwaI          993  ATTTAAAT             ATTT^AAAT
Van91I          9  CCAGGTAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI         1520  GAAGCAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AccI          739  GTCTAC               GT^[AC][GT]AC
              823  GTATAC              
AlwNI         180  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              503  CAGAATCTG           
ApaI          639  GGGCCC               GGGCC^C
             1870  GGGCCC              
AspHI         386  GTGCTC               G[AT]GC[AT]^C
              456  GAGCTC              
BanI         1921  GGTGCC               G^G[CT][GA]CC
             2029  GGTGCC              
BbvCI         130  CCTCAGC              CCTCAGC(-5/-2)
              361  CCTCAGC             
BfaI          959  CTAG                 C^TAG
             3125  CTAG                
BsiHKAI       386  GTGCTC               G[AT]GC[AT]^C
              456  GAGCTC              
BspMI        2121  ACCTGC               ACCTGC(4/8)
             2163  GCAGGT              
DraI          994  TTTAAA               TTT^AAA
             2799  TTTAAA              
MaeI          959  CTAG                 C^TAG
             3125  CTAG                
MspA1I        180  CAGCTG               C[AC]G^C[GT]G
              657  CCGCTG              
SexAI         194  ACCTGGT              A^CC[AT]GGT
             2718  ACCTGGT             
SfcI          614  CTGCAG               C^T[GA][CT]AG
             1210  CTACAG              
SmlI          933  CTCAAG               C^T[CT][GA]AG
             3278  CTCAAG              


#########################################
# 3 OR MORE CUT ENZYMES (   79 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        ApoI        AvaII       BanII       BbsI        
BbvI        BclI        BmyI        BpmI        BpuAI       
BsaJI       BseRI       BsgI        BsiYI       BslI        
BsmAI       Bsp1286I    BspLU11I    BsrI        BssKI       
BstF5I      BstNI       BstXI       BstYI       Cac8I       
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        EcoO109I    EcoRII      EcoT14I     
FbaI        Fnu4HI      FokI        HaeIII      HinfI       
HphI        ItaI        Ksp632I     MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MvaI        MwoI        NcoI        NdeII       NlaIII      
NlaIV       NspI        PleI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XcmI        XhoII       
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