Restriction map for pF1KB5115
 (Linear) (Six-base) MAPSORT of: KIBB5115.seq  Check: 5688  from: 1  to: 3291



KIBB5115.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:37  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2154    3291 
   Size:       2154    1137
 
AccI GT'mk_AC

Cuts at:      0     740     824    3291 
   Size:        740      84    2467

  Fragments arranged by size: 

                2467     740      84
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     530    2665    2912    3291 
   Size:        530    2135     247     379

  Fragments arranged by size: 

                2135     530     379     247
 
AflIII A'CryG_T

Cuts at:      0      86     829    1134    1146    3065    3291 
   Size:         86     743     305      12    1919     226

  Fragments arranged by size: 

                1919     743     305     226      86      12
 
AhdI GACnn_n'nnGTC

Cuts at:      0    3066    3291 
   Size:       3066     225
 
AleI CACnn'nnGTG

Cuts at:      0     769    3291 
   Size:        769    2522
 
AlwNI CAG_nnn'CTG

Cuts at:      0     185     508    3291 
   Size:        185     323    2783

  Fragments arranged by size: 

                2783     323     185
 
ApaI G_GGCC'C

Cuts at:      0     643    1874    3291 
   Size:        643    1231    1417

  Fragments arranged by size: 

                1417    1231     643
 
ApoI r'AATT_y

Cuts at:      0     553    1534    2610    2886    3291 
   Size:        553     981    1076     276     405

  Fragments arranged by size: 

                1076     981     553     405     276
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     732     765    3291 
   Size:        732      33    2526

  Fragments arranged by size: 

                2526     732      33
 
BanI G'GyrC_C

Cuts at:      0    1921    2029    3291 
   Size:       1921     108    1262

  Fragments arranged by size: 

                1921    1262     108
 
BanII G_rGCy'C

Cuts at:      0     460     643    1874    3105    3291 
   Size:        460     183    1231    1231     186

  Fragments arranged by size: 

                1231    1231     460     186     183
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      17     469     952    3291 
   Size:         17     452     483    2339

  Fragments arranged by size: 

                2339     483     452      17
 
BbvCI CC'TCA_GC

Cuts at:      0     131     362    3291 
   Size:        131     231    2929

  Fragments arranged by size: 

                2929     231     131
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3085    3119    3291 
   Size:       3085      34     172

  Fragments arranged by size: 

                3085     172      34
 
BclI T'GATC_A

Cuts at:      0     684    1002    2853    3291 
   Size:        684     318    1851     438

  Fragments arranged by size: 

                1851     684     438     318
 
BglII A'GATC_T

Cuts at:      0     414    3291 
   Size:        414    2877
 
Bme1580I G_kGCm'C

Cuts at:      0     643    1874    1926    2034    2493    3291 
   Size:        643    1231      52     108     459     798

  Fragments arranged by size: 

                1231     798     643     459     108      52
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     173    3291 
   Size:        173    3118
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2595    2890    3291 
   Size:       2595     295     401

  Fragments arranged by size: 

                2595     401     295
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      50     374     455     807    3291 
   Size:         50     324      81     352    2484

  Fragments arranged by size: 

                2484     352     324      81      50
 
Bpu10I CC'TnA_GC

Cuts at:      0     131     362    3167    3291 
   Size:        131     231    2805     124

  Fragments arranged by size: 

                2805     231     131     124
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     918    3263    3291 
   Size:        918    2345      28

  Fragments arranged by size: 

                2345     918      28
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2627    3291 
   Size:       2627     664
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    3164    3194    3291 
   Size:       3164      30      97

  Fragments arranged by size: 

                3164      97      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      47      50    1711    1775    1820    2054    2192    2240 
   Size:         47       3    1661      64      45     234     138      48

Cuts at:   2240    2288    2336    2384    2432    3291 
   Size:         48      48      48      48     859

  Fragments arranged by size: 

                1661     859     234     138      64      48      48      48
                  48      48      47      45       3
 
BseYI C'CCAG_C

Cuts at:      0     721    3291 
   Size:        721    2570
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     593    1029    2948    3291 
   Size:        593     436    1919     343

  Fragments arranged by size: 

                1919     593     436     343
 
BsiHKAI G_wGCw'C

Cuts at:      0     390     460    3291 
   Size:        390      70    2831

  Fragments arranged by size: 

                2831     390      70
 
Bsp1286I G_dGCh'C

Cuts at:      0     390     460     643    1874    1926    2034    2493    3105 
   Size:        390      70     183    1231      52     108     459     612

Cuts at:   3105    3291 
   Size:        186

  Fragments arranged by size: 

                1231     612     459     390     186     183     108      70
                  52
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2130    2154    3291 
   Size:       2130      24    1137

  Fragments arranged by size: 

                2130    1137      24
 
BsrBI CCG'CTC

Cuts at:      0     439    3291 
   Size:        439    2852
 
BsrFI r'CCGG_y

Cuts at:      0     231    3291 
   Size:        231    3060
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     385    3291 
   Size:        385    2906
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2166    2214    2262    2310    2358    3291 
   Size:       2166      48      48      48      48     933

  Fragments arranged by size: 

                2166     933      48      48      48      48
 
BstYI r'GATC_y

Cuts at:      0     414    1810    2574    3291 
   Size:        414    1396     764     717

  Fragments arranged by size: 

                1396     764     717     414
 
BstZ17I GTA'TAC

Cuts at:      0     825    3291 
   Size:        825    2466
 
BtgI C'CryG_G

Cuts at:      0    1156    1898    2006    2069    2114    2207    2255    2351 
   Size:       1156     742     108      63      45      93      48      96

Cuts at:   2351    3291 
   Size:        940

  Fragments arranged by size: 

                1156     940     742     108      96      93      63      48
                  45
 
BtsI GCAGTG_nn'

Cuts at:      0      60     611    2596    2957    3291 
   Size:         60     551    1985     361     334

  Fragments arranged by size: 

                1985     551     361     334      60
 
ClaI AT'CG_AT

Cuts at:      0     678    3291 
   Size:        678    2613
 
DraI TTT'AAA

Cuts at:      0     996    2801    3291 
   Size:        996    1805     490

  Fragments arranged by size: 

                1805     996     490
 
DraIII CAC_nnn'GTG

Cuts at:      0    1096    3291 
   Size:       1096    2195
 
EaeI y'GGCC_r

Cuts at:      0       2     122    3106    3291 
   Size:          2     120    2984     185

  Fragments arranged by size: 

                2984     185     120       2
 
EarI CTCTTCn'nnn_

Cuts at:      0     275     560     612     614    2615    2684    2693    3291 
   Size:        275     285      52       2    2001      69       9     598

  Fragments arranged by size: 

                2001     598     285     275      69      52       9       2
            
 
EcoICRI GAG'CTC

Cuts at:      0     458    3291 
   Size:        458    2833
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      50     374     455     530     807    2665    2912    3291 
   Size:         50     324      81      75     277    1858     247     379

  Fragments arranged by size: 

                1858     379     324     277     247      81      75      50
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0     639     640    1840    1870    1978    2104    3291 
   Size:        639       1    1200      30     108     126    1187

  Fragments arranged by size: 

                1200    1187     639     126     108      30       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2695    3165    3291 
   Size:       2695     470     126

  Fragments arranged by size: 

                2695     470     126
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     765     797    1597    1629    1795    1812    1827    1844 
   Size:        765      32     800      32     166      17      15      17

Cuts at:   1844    3052    3084    3291 
   Size:       1208      32     207

  Fragments arranged by size: 

                1208     800     765     207     166      32      32      32
                  17      17      15
 
HincII GTy'rAC

Cuts at:      0    1575    3291 
   Size:       1575    1716
 
MfeI C'AATT_G

Cuts at:      0    1167    3291 
   Size:       1167    2124
 
MscI TGG'CCA

Cuts at:      0       4    3291 
   Size:          4    3287
 
MslI CAynn'nnrTG

Cuts at:      0      67     769     912     980     996    2164    2212    2260 
   Size:         67     702     143      68      16    1168      48      48

Cuts at:   2260    2308    2356    3038    3291 
   Size:         48      48     682     253

  Fragments arranged by size: 

                1168     702     682     253     143      68      67      48
                  48      48      48      16
 
MspA1I CmG'CkG

Cuts at:      0     182     659    3291 
   Size:        182     477    2632

  Fragments arranged by size: 

                2632     477     182
 
NcoI C'CATG_G

Cuts at:      0    1898    2069    2114    2207    2255    2351    3291 
   Size:       1898     171      45      93      48      96     940

  Fragments arranged by size: 

                1898     940     171      96      93      48      45
 
NdeI CA'TA_TG

Cuts at:      0     965    3291 
   Size:        965    2326
 
NspI r_CATG'y

Cuts at:      0      90     833     883    1138    1150    1909    2478    3069 
   Size:         90     743      50     255      12     759     569     591

Cuts at:   3069    3291 
   Size:        222

  Fragments arranged by size: 

                 759     743     591     569     255     222      90      50
                  12
 
PciI A'CATG_T

Cuts at:      0      86     829    1134    1146    3065    3291 
   Size:         86     743     305      12    1919     226

  Fragments arranged by size: 

                1919     743     305     226      86      12
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      15    3291 
   Size:         15    3276
 
PspOMI G'GGCC_C

Cuts at:      0     639    1870    3291 
   Size:        639    1231    1421

  Fragments arranged by size: 

                1421    1231     639
 
PstI C_TGCA'G

Cuts at:      0     618    3291 
   Size:        618    2673
 
PvuII CAG'CTG

Cuts at:      0     182    3291 
   Size:        182    3109
 
SacI G_AGCT'C

Cuts at:      0     460    3291 
   Size:        460    2831
 
SapI GCTCTTCn'nnn_

Cuts at:      0     612    3291 
   Size:        612    2679
 
SexAI A'CCwGG_T

Cuts at:      0     194    2718    3291 
   Size:        194    2524     573

  Fragments arranged by size: 

                2524     573     194
 
SfcI C'TryA_G

Cuts at:      0     614    1210    3291 
   Size:        614     596    2081

  Fragments arranged by size: 

                2081     614     596
 
SmlI C'TyrA_G

Cuts at:      0     933    3278    3291 
   Size:        933    2345      13

  Fragments arranged by size: 

                2345     933      13
 
SphI G_CATG'C

Cuts at:      0    1909    3291 
   Size:       1909    1382
 
SspI AAT'ATT

Cuts at:      0    1320    3291 
   Size:       1320    1971
 
StyI C'CwwG_G

Cuts at:      0     635     814    1898    2069    2114    2207    2255    2351 
   Size:        635     179    1084     171      45      93      48      96

Cuts at:   2351    3149    3291 
   Size:        798     142

  Fragments arranged by size: 

                1084     798     635     179     171     142      96      93
                  48      45
 
SwaI ATTT'AAAT

Cuts at:      0     996    3291 
   Size:        996    2295
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      22    2017    3291 
   Size:         22    1995    1274

  Fragments arranged by size: 

                1995    1274      22
 
TatI w'GTAC_w

Cuts at:      0    1732    2040    3257    3291 
   Size:       1732     308    1217      34

  Fragments arranged by size: 

                1732    1217     308      34
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      12     117     718    1588    2964    3291 
   Size:         12     105     601     870    1376     327

  Fragments arranged by size: 

                1376     870     601     327     105      12
 
XmnI GAAnn'nnTTC

Cuts at:      0    1524    3291 
   Size:       1524    1767
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     AhdI     AleI    AlwNI     ApaI
     ApoI     BaeI     BanI    BanII     BbsI    BbvCI     BcgI     BclI
    BglII Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI    BsrBI
    BsrFI   BstAPI    BstXI    BstYI  BstZ17I     BtgI     BtsI     ClaI
     DraI   DraIII     EaeI     EarI  EcoICRI  Eco57MI EcoO109I     FalI
    Hin4I   HincII     MfeI     MscI     MslI   MspA1I     NcoI     NdeI
     NspI     PciI    PflMI   PspOMI     PstI    PvuII     SacI     SapI
    SexAI     SfcI     SmlI     SphI     SspI     StyI     SwaI    TaqII
     TatI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AlfI     AloI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII    BamHI     BbeI
    BciVI    BfrBI     BglI     BlpI    BmgBI     BmtI    BsaAI    BsaBI
    BsaHI    BsaWI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrDI    BsrGI   BssHII    BssSI    BstBI   BstEII   Bsu36I    BtgZI
    CspCI     DrdI     EagI     EciI    EcoNI    EcoRI    EcoRV     FseI
     FspI    FspAI    HaeII  HindIII     HpaI     KasI     KpnI     MluI
     MmeI     NaeI     NarI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI
     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SrfI     StuI     TstI  Tth111I     XbaI     XhoI     XmaI
     ZraI
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