Restriction map for pF1KB5025
##################################
# NO CUT ENZYMES (  154 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AviII       AvrII       BamHI       BbrPI       
BbvCI       BciVI       BfaI        BfrI        BglI        
BglII       BlnI        BsaAI       BsaBI       BsaI        
BsaWI       BseAI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmAI       BsmBI       BsmFI       BsmI        
Bsp1407I    BspEI       BspHI       BspLU11I    BspMI       
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstXI       
BstYI       BstZ17I     Bsu36I      CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoO65I     EcoRI       
EcoRV       EcoT22I     FseI        FspI        HincII      
HindII      HinfI       HpaI        HphI        KpnI        
MaeI        MaeII       MaeIII      MamI        MfeI        
MflI        MluI        MroI        MseI        MslI        
MunI        NcoI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsaI        RsrII       SacI        
SalI        SbfI        ScaI        SexAI       SfaNI       
SfiI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TfiI        Tru9I       Tsp45I      
Tsp509I     Tth111I     Van91I      XbaI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AcyI           88  GGCGCC               G[GA]^CG[CT]C
ApaI          368  GGGCCC               GGGCC^C
BalI            2  TGGCCA               TGG^CCA
BbeI           88  GGCGCC               GGCGC^C
BbsI          229  GTCTTC               GAAGAC(2/6)
BcgI          198  CGAGGGCGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          401  TGATCA               T^GATCA
BpuAI         229  GTCTTC               GAAGAC(2/6)
BsaHI          88  GGCGCC               G[GA]^CG[CT]C
BspDI         532  ATCGAT               AT^CGAT
BsrBI         643  GAGCGG               CCGCTC(-3/-3)
ClaI          532  ATCGAT               AT^CGAT
EarI          393  GAAGAG               CTCTTC(1/4)
EcoNI         270  CCTCATCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       581  CCAAGG               C^C[AT][AT]GG
FbaI          401  TGATCA               T^GATCA
Hin1I          88  GGCGCC               G[GA]^CG[CT]C
HindIII       520  AAGCTT               A^AGCTT
KasI           88  GGCGCC               G^GCGCC
Ksp632I       393  GAAGAG               CTCTTC(1/4)
KspI          473  CCGCGG               CCGC^GG
MluNI           2  TGGCCA               TGG^CCA
MscI            2  TGGCCA               TGG^CCA
NarI           88  GGCGCC               GG^CGCC
NspI          290  GCATGT               [GA]CATG^[CT]
PpuMI         278  AGGACCT              [GA]G^G[AT]CC[CT]
SacII         473  CCGCGG               CCGC^GG
SapI          393  GAAGAGC              GCTCTTC(1/4)
SfoI           88  GGCGCC               GGC^GCC
SmlI          697  CTCAAG               C^T[CT][GA]AG
StyI          581  CCAAGG               C^C[AT][AT]GG
XcmI            5  CCAGTGAGGAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AvaI          112  CCCGAG               C^[CT]CG[GA]G
              118  CCCGAG              
AvaII         279  GGACC                G^G[AT]CC
              585  GGTCC               
BanI           88  GGCGCC               G^G[CT][GA]CC
              559  GGTGCC              
BsoBI         112  CCCGAG               C^[CT]CG[GA]G
              118  CCCGAG              
BsrFI          35  GCCGGC               [GA]^CCGG[CT]
              179  GCCGGC              
BsrI            5  CCAGT                ACTGG(1/-1)
              605  CCAGT               
BstF5I        318  GGATG                GGATG(2/0)
              414  GGATG               
Cfr10I         35  GCCGGC               [GA]^CCGG[CT]
              179  GCCGGC              
DpnII         402  GATC                 ^GATC
              531  GATC                
DsaI          140  CCACGG               C^C[GA][CT]GG
              473  CCGCGG              
FokI          318  GGATG                GGATG(9/13)
              414  GGATG               
HgaI          154  GACGC                GACGC(5/10)
              307  GACGC               
MboI          402  GATC                 ^GATC
              531  GATC                
MboII         393  GAAGA                GAAGA(8/7)
              230  TCTTC               
NaeI           35  GCCGGC               GCC^GGC
              179  GCCGGC              
NdeII         402  GATC                 ^GATC
              531  GATC                
NgoMIV         35  GCCGGC               G^CCGGC
              179  GCCGGC              
NlaIII        291  CATG                 CATG^
              399  CATG                
PstI          222  CTGCAG               CTGCA^G
              487  CTGCAG              
PvuII          73  CAGCTG               CAG^CTG
              172  CAGCTG              
Sau3AI        402  GATC                 ^GATC
              531  GATC                
SfcI          222  CTGCAG               C^T[GA][CT]AG
              487  CTGCAG              
TspRI           6  CAGTG                CA[GC]TG[ACGT][ACGT]^
              498  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   49 enzymes) #
#########################################

AccII       AciI        AluI        BanII       BbvI        
BcnI        BlpI        BmyI        BpmI        Bpu1102I    
BsaJI       BseRI       BsiYI       BslI        Bsp1286I    
BssKI       BstNI       BstUI       Cac8I       CelII       
CfoI        Cfr13I      DdeI        DraII       EaeI        
EcoO109I    EcoRII      FauI        Fnu4HI      HaeII       
HaeIII      HapII       HhaI        HinP1I      HpaII       
ItaI        MnlI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NlaIV       Sau96I      
ScrFI       TaqI        TseI        TthHB8I     
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com