Restriction map for pF1KB5025
 (Linear) (Six-base) MAPSORT of: KIBB5025.seq  Check: 4785  from: 1  to: 717



KIBB5025.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:37  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     364     412     717 
   Size:        364      48     305

  Fragments arranged by size: 

                 364     305      48
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     277     309     717 
   Size:        277      32     408

  Fragments arranged by size: 

                 408     277      32
 
ApaI G_GGCC'C

Cuts at:      0     372     717 
   Size:        372     345
 
AvaI C'yCGr_G

Cuts at:      0     112     118     717 
   Size:        112       6     599

  Fragments arranged by size: 

                 599     112       6
 
BanI G'GyrC_C

Cuts at:      0      88     559     717 
   Size:         88     471     158

  Fragments arranged by size: 

                 471     158      88
 
BanII G_rGCy'C

Cuts at:      0     107     119     372     717 
   Size:        107      12     253     345

  Fragments arranged by size: 

                 345     253     107      12
 
BbeI G_GCGC'C

Cuts at:      0      92     717 
   Size:         92     625
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     222     717 
   Size:        222     495
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     187     221     717 
   Size:        187      34     496

  Fragments arranged by size: 

                 496     187      34
 
BclI T'GATC_A

Cuts at:      0     401     717 
   Size:        401     316
 
BlpI GC'TnA_GC

Cuts at:      0     163     175     436     632     717 
   Size:        163      12     261     196      85

  Fragments arranged by size: 

                 261     196     163      85      12
 
Bme1580I G_kGCm'C

Cuts at:      0     372     717 
   Size:        372     345
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     304     565     717 
   Size:         49     255     261     152

  Fragments arranged by size: 

                 261     255     152      49
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     682     717 
   Size:        682      35
 
BsaHI Gr'CG_yC

Cuts at:      0      89     717 
   Size:         89     628
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0       4      34     277     307     717 
   Size:          4      30     243      30     410

  Fragments arranged by size: 

                 410     243      30      30       4
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      25      64      73     670     673     676     717 
   Size:         25      39       9     597       3       3      41

  Fragments arranged by size: 

                 597      41      39      25       9       3       3
 
BseYI C'CCAG_C

Cuts at:      0      71      93     170     310     363     717 
   Size:         71      22      77     140      53     354

  Fragments arranged by size: 

                 354     140      77      71      53      22
 
Bsp1286I G_dGCh'C

Cuts at:      0     107     119     372     717 
   Size:        107      12     253     345

  Fragments arranged by size: 

                 345     253     107      12
 
BsrBI CCG'CTC

Cuts at:      0     645     717 
   Size:        645      72
 
BsrFI r'CCGG_y

Cuts at:      0      35     179     717 
   Size:         35     144     538

  Fragments arranged by size: 

                 538     144      35
 
BtgI C'CryG_G

Cuts at:      0     140     473     717 
   Size:        140     333     244

  Fragments arranged by size: 

                 333     244     140
 
ClaI AT'CG_AT

Cuts at:      0     533     717 
   Size:        533     184
 
EaeI y'GGCC_r

Cuts at:      0       2     143     602     717 
   Size:          2     141     459     115

  Fragments arranged by size: 

                 459     141     115       2
 
EarI CTCTTCn'nnn_

Cuts at:      0     388     717 
   Size:        388     329
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     664     717 
   Size:        664      53
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     304     364     412     565     717 
   Size:         49     255      60      48     153     152

  Fragments arranged by size: 

                 255     153     152      60      49      48
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     274     717 
   Size:        274     443
 
EcoO109I rG'GnC_Cy

Cuts at:      0     279     368     369     717 
   Size:        279      89       1     348

  Fragments arranged by size: 

                 348     279      89       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     456     588     717 
   Size:        456     132     129

  Fragments arranged by size: 

                 456     132     129
 
HaeII r_GCGC'y

Cuts at:      0      36      92     152     170     206     222     514     717 
   Size:         36      56      60      18      36      16     292     203

  Fragments arranged by size: 

                 292     203      60      56      36      36      18      16
            
 
HindIII A'AGCT_T

Cuts at:      0     520     717 
   Size:        520     197
 
KasI G'GCGC_C

Cuts at:      0      88     717 
   Size:         88     629
 
MscI TGG'CCA

Cuts at:      0       4     717 
   Size:          4     713
 
MspA1I CmG'CkG

Cuts at:      0      75     174     475     717 
   Size:         75      99     301     242

  Fragments arranged by size: 

                 301     242      99      75
 
NaeI GCC'GGC

Cuts at:      0      37     181     717 
   Size:         37     144     536

  Fragments arranged by size: 

                 536     144      37
 
NarI GG'CG_CC

Cuts at:      0      89     717 
   Size:         89     628
 
NgoMIV G'CCGG_C

Cuts at:      0      35     179     717 
   Size:         35     144     538

  Fragments arranged by size: 

                 538     144      35
 
NspI r_CATG'y

Cuts at:      0     294     717 
   Size:        294     423
 
PfoI T'CCnGG_A

Cuts at:      0     251     443     717 
   Size:        251     192     274

  Fragments arranged by size: 

                 274     251     192
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     277     309     717 
   Size:        277      32     408

  Fragments arranged by size: 

                 408     277      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     279     717 
   Size:        279     438
 
PspOMI G'GGCC_C

Cuts at:      0     368     717 
   Size:        368     349
 
PstI C_TGCA'G

Cuts at:      0     226     491     717 
   Size:        226     265     226

  Fragments arranged by size: 

                 265     226     226
 
PvuII CAG'CTG

Cuts at:      0      75     174     717 
   Size:         75      99     543

  Fragments arranged by size: 

                 543      99      75
 
SacII CC_GC'GG

Cuts at:      0     476     717 
   Size:        476     241
 
SapI GCTCTTCn'nnn_

Cuts at:      0     388     717 
   Size:        388     329
 
SfcI C'TryA_G

Cuts at:      0     222     487     717 
   Size:        222     265     230

  Fragments arranged by size: 

                 265     230     222
 
SfoI GGC'GCC

Cuts at:      0      90     717 
   Size:         90     627
 
SmlI C'TyrA_G

Cuts at:      0     697     717 
   Size:        697      20
 
StyI C'CwwG_G

Cuts at:      0     581     717 
   Size:        581     136
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0       4      36     717 
   Size:          4      32     681

  Fragments arranged by size: 

                 681      32       4
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      12     717 
   Size:         12     705
 

 Enzymes that do cut:

     AcuI     AloI     ApaI     AvaI     BanI    BanII     BbeI     BbsI
     BcgI     BclI     BlpI Bme1580I     BpmI    BpuEI    BsaHI    BsaXI
    BseRI    BseYI Bsp1286I    BsrBI    BsrFI     BtgI     ClaI     EaeI
     EarI     EciI  Eco57MI    EcoNI EcoO109I     FalI    HaeII  HindIII
     KasI     MscI   MspA1I     NaeI     NarI   NgoMIV     NspI     PfoI
     PpiI    PpuMI   PspOMI     PstI    PvuII    SacII     SapI     SfcI
     SfoI     SmlI     StyI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI    BbvCI    BciVI    BfrBI
     BglI    BglII    BmgBI     BmrI     BmtI     BplI   Bpu10I     BsaI
    BsaAI    BsaBI    BsaWI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrDI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI
     BtsI    CspCI     DraI   DraIII     DrdI     EagI  EcoICRI    EcoRI
    EcoRV     FseI     FspI    FspAI    Hin4I   HincII     HpaI     KpnI
     MfeI     MluI     MmeI     MslI     NcoI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI    PflMI     PmeI     PmlI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SacI     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TatI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com