Restriction map for pF1KB5016
##################################
# NO CUT ENZYMES (  130 enzymes) #
##################################

Acc65I      AccI        AccII       AccIII      AclI        
AfaI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AlwNI       ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AviII       AvrII       BamHI       BanI        BbeI        
BbvCI       BcgI        BciVI       BclI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BsaBI       
BsaWI       BseAI       BsgI        BsiEI       BsiWI       
BsmBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrGI       BssHII      Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstYI       
BstZ17I     CelII       ClaI        CpoI        DraI        
EagI        Eam1105I    EclXI       Eco52I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MflI        MluI        MroI        MunI        MvnI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PflFI       PflMI       PinAI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsaI        RsrII       SacI        SacII       SalI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
SspBI       SspI        StuI        SwaI        Tth111I     
Van91I      XbaI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AatII         199  GACGTC               GACGT^C
AocI         1060  CCTGAGG              CC^T[ACGT]AGG
ApaI         1056  GGGCCC               GGGCC^C
AspHI        1890  GAGCAC               G[AT]GC[AT]^C
AvaI         1639  CTCGAG               C^[CT]CG[GA]G
BbrPI        1277  CACGTG               CAC^GTG
BfaI         1496  CTAG                 C^TAG
BglI         2021  GCCAAAGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI        1277  CACGTG               [CT]AC^GT[GA]
BsiHKAI      1890  GAGCAC               G[AT]GC[AT]^C
BsmI          669  GCATTC               GAATGC(1/-1)
BsoBI        1639  CTCGAG               C^[CT]CG[GA]G
BspMI        1463  ACCTGC               ACCTGC(4/8)
BsrBI         398  GAGCGG               CCGCTC(-3/-3)
BsrDI        1981  GCAATG               GCAATG(2/0)
BsrFI        1884  GCCGGT               [GA]^CCGG[CT]
Bsu36I       1060  CCTGAGG              CC^T[ACGT]AGG
Cfr10I       1884  GCCGGT               [GA]^CCGG[CT]
DpnII         647  GATC                 ^GATC
DraIII       1379  CACAAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco81I       1060  CCTGAGG              CC^T[ACGT]AGG
EcoT14I       336  CCAAGG               C^C[AT][AT]GG
FauI          380  GCGGG                CCCGC(4/6)
MaeI         1496  CTAG                 C^TAG
MaeIII         97  GTGAC                ^GT[ACGT]AC
MboI          647  GATC                 ^GATC
MseI          256  TTAA                 T^TAA
NdeII         647  GATC                 ^GATC
NspI          657  ACATGC               [GA]CATG^[CT]
PaeR7I       1639  CTCGAG               C^TCGAG
PmaCI        1277  CACGTG               CAC^GTG
PmlI         1277  CACGTG               CAC^GTG
PstI         1465  CTGCAG               CTGCA^G
Sau3AI        647  GATC                 ^GATC
SbfI         1464  CCTGCAGG             CCTGCA^GG
Sse8387I     1464  CCTGCAGG             CCTGCA^GG
StyI          336  CCAAGG               C^C[AT][AT]GG
Tru9I         256  TTAA                 T^TAA
Tsp45I         97  GTGAC                ^GT[GC]AC
XhoI         1639  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AcsI         1680  GAATTT               [GA]^AATT[CT]
             2014  AAATTC              
AcyI          199  GACGTC               G[GA]^CG[CT]C
             1048  GGCGTC              
Aor51HI       145  AGCGCT               AGC^GCT
             1079  AGCGCT              
ApoI         1680  GAATTT               [GA]^AATT[CT]
             2014  AAATTC              
BalI          641  TGGCCA               TGG^CCA
              953  TGGCCA              
BcnI          665  CCGGG                CC^[GC]GG
             1237  CCGGG               
BsaHI         199  GACGTC               G[GA]^CG[CT]C
             1048  GGCGTC              
BsaI          260  GAGACC               GGTCTC(1/5)
             1460  GAGACC              
BssSI         286  CACGAG               CACGAG(-5/-1)
              834  CACGAG              
CfoI          146  GCGC                 GCG^C
             1080  GCGC                
DrdI          433  GACAGCCAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1930  GACCAGACAGTC        
EaeI          641  TGGCCA               [CT]^GGCC[GA]
              953  TGGCCA              
Eco47III      145  AGCGCT               AGC^GCT
             1079  AGCGCT              
EcoNI         293  CCTCCAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1941  CCTGACAGAGG         
HaeII         145  AGCGCT               [GA]GCGC^[CT]
             1079  AGCGCT              
HgaI          830  GACGC                GACGC(5/10)
             1049  GCGTC               
HhaI          146  GCGC                 GCG^C
             1080  GCGC                
Hin1I         199  GACGTC               G[GA]^CG[CT]C
             1048  GGCGTC              
HinP1I        146  GCGC                 G^CGC
             1080  GCGC                
MluNI         641  TGGCCA               TGG^CCA
              953  TGGCCA              
MscI          641  TGGCCA               TGG^CCA
              953  TGGCCA              
NciI          665  CCGGG                CC^[GC]GG
             1237  CCGGG               
XcmI          809  CCACCTCTGAGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1378  CCACAAGGTGCGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   65 enzymes) #
#########################################

AciI        AluI        Asp700I     AvaII       BanII       
BbsI        BbvI        BmyI        BpmI        BpuAI       
BsaJI       BseRI       BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstXI       Cac8I       Cfr13I      DdeI        
DraII       DsaI        EarI        EcoO109I    EcoRII      
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeII       
MboII       MnlI        MslI        MspA1I      MspI        
MvaI        MwoI        NlaIII      NlaIV       PleI        
PpuMI       PvuII       SapI        Sau96I      ScrFI       
SfaNI       SfcI        SmlI        TaqI        TfiI        
TseI        Tsp509I     TspRI       TthHB8I     XmnI        
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