Restriction map for pF1KB5016
 (Linear) (Six-base) MAPSORT of: KIBB5016.seq  Check: 4209  from: 1  to: 2031



KIBB5016.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:37  ..

AatII G_ACGT'C

Cuts at:      0     203    2031 
   Size:        203    1828
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     202     467     599     986    2031 
   Size:        202     265     132     387    1045

  Fragments arranged by size: 

                1045     387     265     202     132
 
AfeI AGC'GCT

Cuts at:      0     147    1081    2031 
   Size:        147     934     950

  Fragments arranged by size: 

                 950     934     147
 
AleI CACnn'nnGTG

Cuts at:      0    1337    2031 
   Size:       1337     694
 
ApaI G_GGCC'C

Cuts at:      0    1060    2031 
   Size:       1060     971
 
ApoI r'AATT_y

Cuts at:      0    1680    2014    2031 
   Size:       1680     334      17

  Fragments arranged by size: 

                1680     334      17
 
AvaI C'yCGr_G

Cuts at:      0    1639    2031 
   Size:       1639     392
 
BanII G_rGCy'C

Cuts at:      0     318     410    1060    1116    1437    2031 
   Size:        318      92     650      56     321     594

  Fragments arranged by size: 

                 650     594     321     318      92      56
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     190     463     688     886    2031 
   Size:        190     273     225     198    1145

  Fragments arranged by size: 

                1145     273     225     198     190
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2027    2031 
   Size:       2027       4
 
Bme1580I G_kGCm'C

Cuts at:      0     386    1060    2031 
   Size:        386     674     971

  Fragments arranged by size: 

                 971     674     386
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     405    1752    2031 
   Size:        405    1347     279

  Fragments arranged by size: 

                1347     405     279
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     138    2031 
   Size:        138    1893
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     279    1090    1570    2031 
   Size:        279     811     480     461

  Fragments arranged by size: 

                 811     480     461     279
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     866    1252    2031 
   Size:        866     386     779

  Fragments arranged by size: 

                 866     779     386
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     254    1454    2031 
   Size:        254    1200     577

  Fragments arranged by size: 

                1200     577     254
 
BsaAI yAC'GTr

Cuts at:      0    1279    2031 
   Size:       1279     752
 
BsaHI Gr'CG_yC

Cuts at:      0     200    1049    2031 
   Size:        200     849     982

  Fragments arranged by size: 

                 982     849     200
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     277     307    2031 
   Size:        277      30    1724

  Fragments arranged by size: 

                1724     277      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     340     508     511     514     517     646     736     873 
   Size:        340     168       3       3       3     129      90     137

Cuts at:    873     949    1147    1162    1663    1894    2031 
   Size:         76     198      15     501     231     137

  Fragments arranged by size: 

                 501     340     231     198     168     137     137     129
                  90      76      15       3       3       3
 
BseYI C'CCAG_C

Cuts at:      0     264    1168    2031 
   Size:        264     904     863

  Fragments arranged by size: 

                 904     863     264
 
BsiHKAI G_wGCw'C

Cuts at:      0    1894    2031 
   Size:       1894     137
 
BsmI GAATG_Cn'

Cuts at:      0     669    2031 
   Size:        669    1362
 
Bsp1286I G_dGCh'C

Cuts at:      0     318     386     410    1060    1116    1437    1894    2031 
   Size:        318      68      24     650      56     321     457     137

  Fragments arranged by size: 

                 650     457     321     318     137      68      56      24
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1472    2031 
   Size:       1472     559
 
BsrBI CCG'CTC

Cuts at:      0     400    2031 
   Size:        400    1631
 
BsrDI GCAATG_nn'

Cuts at:      0    1988    2031 
   Size:       1988      43
 
BsrFI r'CCGG_y

Cuts at:      0    1884    2031 
   Size:       1884     147
 
BssSI C'ACGA_G

Cuts at:      0     286     834    2031 
   Size:        286     548    1197

  Fragments arranged by size: 

                1197     548     286
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      66     715    1445    2031 
   Size:         66     649     730     586

  Fragments arranged by size: 

                 730     649     586      66
 
Bsu36I CC'TnA_GG

Cuts at:      0    1061    2031 
   Size:       1061     970
 
BtgI C'CryG_G

Cuts at:      0      35     275     852    2031 
   Size:         35     240     577    1179

  Fragments arranged by size: 

                1179     577     240      35
 
BtsI GCAGTG_nn'

Cuts at:      0     552    2031 
   Size:        552    1479
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1369    1404    2031 
   Size:       1369      35     627

  Fragments arranged by size: 

                1369     627      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    1384    2031 
   Size:       1384     647
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     439    1936    2031 
   Size:        439    1497      95

  Fragments arranged by size: 

                1497     439      95
 
EaeI y'GGCC_r

Cuts at:      0     641     953    2031 
   Size:        641     312    1078

  Fragments arranged by size: 

                1078     641     312
 
EarI CTCTTCn'nnn_

Cuts at:      0     469     545     560     694    1214    1301    1540    1588 
   Size:        469      76      15     134     520      87     239      48

Cuts at:   1588    1608    1750    2031 
   Size:         20     142     281

  Fragments arranged by size: 

                 520     469     281     239     142     134      87      76
                  48      20      15
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     202     279     467     599     986    1090    1570    2031 
   Size:        202      77     188     132     387     104     480     461

  Fragments arranged by size: 

                 480     461     387     202     188     132     104      77
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     297    1945    2031 
   Size:        297    1648      86

  Fragments arranged by size: 

                1648     297      86
 
EcoO109I rG'GnC_Cy

Cuts at:      0     117     875    1056    1057    1065    1227    1773    2031 
   Size:        117     758     181       1       8     162     546     258

  Fragments arranged by size: 

                 758     546     258     181     162     117       8       1
            
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1701    2031 
   Size:       1701     330
 
HaeII r_GCGC'y

Cuts at:      0     149    1083    2031 
   Size:        149     934     948

  Fragments arranged by size: 

                 948     934     149
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     408     440     796     828     862     894    2031 
   Size:        408      32     356      32      34      32    1137

  Fragments arranged by size: 

                1137     408     356      34      32      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     455    2031 
   Size:        455    1576
 
MscI TGG'CCA

Cuts at:      0     643     955    2031 
   Size:        643     312    1076

  Fragments arranged by size: 

                1076     643     312
 
MslI CAynn'nnrTG

Cuts at:      0      64    1199    1337    2031 
   Size:         64    1135     138     694

  Fragments arranged by size: 

                1135     694     138      64
 
MspA1I CmG'CkG

Cuts at:      0     268    1534    1852    2031 
   Size:        268    1266     318     179

  Fragments arranged by size: 

                1266     318     268     179
 
NspI r_CATG'y

Cuts at:      0     661    2031 
   Size:        661    1370
 
PasI CC'CwG_GG

Cuts at:      0     319    1438    2031 
   Size:        319    1119     593

  Fragments arranged by size: 

                1119     593     319
 
PmlI CAC'GTG

Cuts at:      0    1279    2031 
   Size:       1279     752
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1300    1332    2031 
   Size:       1300      32     699

  Fragments arranged by size: 

                1300     699      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     117     875    1065    1773    2031 
   Size:        117     758     190     708     258

  Fragments arranged by size: 

                 758     708     258     190     117
 
PspOMI G'GGCC_C

Cuts at:      0    1056    2031 
   Size:       1056     975
 
PstI C_TGCA'G

Cuts at:      0    1469    2031 
   Size:       1469     562
 
PvuII CAG'CTG

Cuts at:      0     268    1534    1852    2031 
   Size:        268    1266     318     179

  Fragments arranged by size: 

                1266     318     268     179
 
SanDI GG'GwC_CC

Cuts at:      0    1773    2031 
   Size:       1773     258
 
SapI GCTCTTCn'nnn_

Cuts at:      0     469     560    1588    2031 
   Size:        469      91    1028     443

  Fragments arranged by size: 

                1028     469     443      91
 
SbfI CC_TGCA'GG

Cuts at:      0    1469    2031 
   Size:       1469     562
 
SfcI C'TryA_G

Cuts at:      0     993    1083    1465    1836    1996    2031 
   Size:        993      90     382     371     160      35

  Fragments arranged by size: 

                 993     382     371     160      90      35
 
SmlI C'TyrA_G

Cuts at:      0     881    1231    1639    2031 
   Size:        881     350     408     392

  Fragments arranged by size: 

                 881     408     392     350
 
StyI C'CwwG_G

Cuts at:      0     336    2031 
   Size:        336    1695
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     168    1312    1909    2031 
   Size:        168    1144     597     122

  Fragments arranged by size: 

                1144     597     168     122
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     277     309    1057    1089    2031 
   Size:        277      32     748      32     942

  Fragments arranged by size: 

                 942     748     277      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     816    1385    2031 
   Size:        816     569     646

  Fragments arranged by size: 

                 816     646     569
 
XhoI C'TCGA_G

Cuts at:      0    1639    2031 
   Size:       1639     392
 
XmnI GAAnn'nnTTC

Cuts at:      0    1315    1681    1684    2031 
   Size:       1315     366       3     347

  Fragments arranged by size: 

                1315     366     347       3
 
ZraI GAC'GTC

Cuts at:      0     201    2031 
   Size:        201    1830
 

 Enzymes that do cut:

    AatII     AcuI     AfeI     AleI     ApaI     ApoI     AvaI    BanII
     BbsI     BglI Bme1580I     BmrI     BplI     BpmI    BpuEI     BsaI
    BsaAI    BsaHI    BsaXI    BseRI    BseYI  BsiHKAI     BsmI Bsp1286I
    BspMI    BsrBI    BsrDI    BsrFI    BssSI    BstXI   Bsu36I     BtgI
     BtsI    CspCI   DraIII     DrdI     EaeI     EarI  Eco57MI    EcoNI
 EcoO109I     FalI    HaeII    Hin4I     MmeI     MscI     MslI   MspA1I
     NspI     PasI     PmlI     PpiI    PpuMI   PspOMI     PstI    PvuII
    SanDI     SapI     SbfI     SfcI     SmlI     StyI    TaqII     TstI
     XcmI     XhoI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AlfI     AloI    AlwNI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI     BclI
    BfrBI    BglII     BlpI    BmgBI     BmtI   Bpu10I    BsaBI    BsaWI
     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BsrGI   BssHII
   BstAPI    BstBI   BstEII    BstYI  BstZ17I    BtgZI     ClaI     DraI
     EagI     EciI  EcoICRI    EcoRI    EcoRV     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PciI    PflMI     PfoI     PmeI    PshAI     PsiI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI     TatI  Tth111I     XbaI     XmaI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com