Restriction map for pF1KB4920
##################################
# NO CUT ENZYMES (  136 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AciI        
AclI        AcyI        AgeI        AlwNI       AocI        
Aor51HI     ApaI        AscI        Asp718I     AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BbeI        BbrPI       BbvCI       BclI        BcnI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       BsoBI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrFI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstUI       BstYI       BstZ17I     Bsu36I      CelII       
Cfr10I      CpoI        DraIII      DrdI        EaeI        
EagI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoRV       FauI        FbaI        FseI        
FspI        HaeII       HapII       Hin1I       HpaI        
HpaII       KasI        KpnI        KspI        MamI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MspA1I      MspI        MunI        MvnI        
NaeI        NarI        NciI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacII       SapI        
SbfI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspI        StuI        SwaI        Tth111I     
Van91I      XcmI        XhoI        XhoII       XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AflII         223  CTTAAG               C^TTAAG
AflIII       2253  ACGTGT               A^C[GA][CT]GT
AhdI         1545  GACTTTCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI          913  GATCC                GGATC(4/5)
AseI         1486  ATTAAT               AT^TAAT
AsnI         1486  ATTAAT               AT^TAAT
AspEI        1545  GACTTTCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI         2266  GGTGCC               G^G[CT][GA]CC
BanII         214  GAGCTC               G[GA]GC[CT]^C
BcgI          211  CGAGAGCTCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         336  GTATCC               GTATCC(6/5)
BfrI          223  CTTAAG               C^TTAAG
BseRI         404  CTCCTC               GAGGAG(10/8)
BsgI          568  GTGCAG               GTGCAG(16/14)
BsiYI         171  CCCAGACCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          171  CCCAGACCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspDI         504  ATCGAT               AT^CGAT
BstEII        492  GGTGACC              G^GT[ACGT]ACC
BstPI         492  GGTGACC              G^GT[ACGT]ACC
BstXI        1278  CCACCAGTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI         1979  GCGC                 GCG^C
ClaI          504  ATCGAT               AT^CGAT
DpnII         913  GATC                 ^GATC
DraI         1942  TTTAAA               TTT^AAA
DraII         702  AGGCCCT              [GA]G^G[ACGT]CC[CT]
DsaI          673  CCATGG               C^C[GA][CT]GG
Eam1105I     1545  GACTTTCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          394  GAAGAG               CTCTTC(1/4)
EcoO109I      702  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I       492  GGTGACC              G^GT[ACGT]ACC
EcoT14I       673  CCATGG               C^C[AT][AT]GG
EcoT22I      2195  ATGCAT               ATGCA^T
HaeIII        703  GGCC                 GG^CC
HgaI          561  GCGTC                GACGC(5/10)
HhaI         1979  GCGC                 GCG^C
HinP1I       1979  GCGC                 G^CGC
HindIII      2048  AAGCTT               A^AGCTT
Ksp632I       394  GAAGAG               CTCTTC(1/4)
MboI          913  GATC                 ^GATC
NcoI          673  CCATGG               C^CATGG
NdeII         913  GATC                 ^GATC
NsiI         2195  ATGCAT               ATGCA^T
PshAI        1378  GACTTCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        1486  ATTAAT               AT^TAAT
SacI          214  GAGCTC               GAGCT^C
SalI          143  GTCGAC               G^TCGAC
Sau3AI        913  GATC                 ^GATC
ScaI         1642  AGTACT               AGT^ACT
StyI          673  CCATGG               C^C[AT][AT]GG
XbaI          390  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AccI          143  GTCGAC               GT^[AC][GT]AC
              529  GTAGAC              
AvaII          82  GGACC                G^G[AT]CC
              184  GGTCC               
BbvI          347  GCAGC                GCAGC(8/12)
              270  GCTGC               
BsaJI         539  CCCAGG               C^C[ACGT][ACGT]GG
              673  CCATGG              
BsmFI         627  GGGAC                GGGAC(10/14)
              330  GTCCC               
BsrDI        1529  GCAATG               GCAATG(2/0)
             1613  GCAATG              
Fnu4HI        270  GCTGC                GC^[ACGT]GC
              347  GCAGC               
HincII        143  GTCGAC               GT[CT]^[GA]AC
              203  GTCAAC              
HindII        143  GTCGAC               GT[CT]^[GA]AC
              203  GTCAAC              
ItaI          270  GCTGC                GC^[ACGT]GC
              347  GCAGC               
MwoI          270  GCTGCTGGTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              561  GCGTCCTGTGC         
NlaIV          82  GGACCC               GG[ACGT]^[ACGT]CC
             2266  GGTGCC              
SfcI          527  CTGTAG               C^T[GA][CT]AG
              917  CTACAG              
SmlI            9  CTCAAG               C^T[CT][GA]AG
              223  CTTAAG              
TseI          270  GCTGC                G^C[AT]GC
              347  GCAGC               


#########################################
# 3 OR MORE CUT ENZYMES (   57 enzymes) #
#########################################

AcsI        AfaI        AluI        Alw44I      ApaLI       
ApoI        Asp700I     AspHI       BbsI        BfaI        
BmyI        BpmI        BpuAI       BsaI        BsiHKAI     
BsmAI       BsmI        Bsp1286I    Bsp1407I    BsrGI       
BsrI        BssKI       BstF5I      BstNI       Cac8I       
Cfr13I      DdeI        EcoRI       EcoRII      FokI        
HinfI       HphI        MaeI        MaeII       MaeIII      
MboII       MnlI        MseI        MslI        MvaI        
NdeI        NlaIII      NspI        PleI        RsaI        
Sau96I      ScrFI       SfaNI       SspBI       TaqI        
TfiI        Tru9I       Tsp45I      Tsp509I     TspRI       
TthHB8I     XmnI        
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