Restriction map for pF1KB4920
 (Linear) (Six-base) MAPSORT of: KIBB4920.seq  Check: 4558  from: 1  to: 2283



KIBB4920.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:37  ..

AccI GT'mk_AC

Cuts at:      0     144     530    2283 
   Size:        144     386    1753

  Fragments arranged by size: 

                1753     386     144
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1281    1365    1449    1555    1785    2283 
   Size:       1281      84      84     106     230     498

  Fragments arranged by size: 

                1281     498     230     106      84      84
 
AflII C'TTAA_G

Cuts at:      0     223    2283 
   Size:        223    2060
 
AflIII A'CryG_T

Cuts at:      0    2253    2283 
   Size:       2253      30
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1550    2283 
   Size:       1550     733
 
AleI CACnn'nnGTG

Cuts at:      0    1283    2283 
   Size:       1283    1000
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      12      44    2283 
   Size:         12      32    2239

  Fragments arranged by size: 

                2239      32      12
 
ApaLI G'TGCA_C

Cuts at:      0    1307    1391    1897    2283 
   Size:       1307      84     506     386

  Fragments arranged by size: 

                1307     506     386      84
 
ApoI r'AATT_y

Cuts at:      0     150     159     260     692     857    1126    1331    1667 
   Size:        150       9     101     432     165     269     205     336

Cuts at:   1667    2171    2283 
   Size:        504     112

  Fragments arranged by size: 

                 504     432     336     269     205     165     150     112
                 101       9
 
AseI AT'TA_AT

Cuts at:      0    1487    2283 
   Size:       1487     796
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1146    1179    2283 
   Size:       1146      33    1104

  Fragments arranged by size: 

                1146    1104      33
 
BanI G'GyrC_C

Cuts at:      0    2266    2283 
   Size:       2266      17
 
BanII G_rGCy'C

Cuts at:      0     218    2283 
   Size:        218    2065
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     449     806    1549    2283 
   Size:        449     357     743     734

  Fragments arranged by size: 

                 743     734     449     357
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     200     234    2283 
   Size:        200      34    2049

  Fragments arranged by size: 

                2049     200      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     347    2283 
   Size:        347    1936
 
BfrBI ATG'CAT

Cuts at:      0    2197    2283 
   Size:       2197      86
 
Bme1580I G_kGCm'C

Cuts at:      0    1311    1395    1901    2283 
   Size:       1311      84     506     382

  Fragments arranged by size: 

                1311     506     382      84
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     327     558    1601    2283 
   Size:        327     231    1043     682

  Fragments arranged by size: 

                1043     682     327     231
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     264    2283 
   Size:        264    2019
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     301     728    1109    1277    1361    1445    1529    1697 
   Size:        301     427     381     168      84      84      84     168

Cuts at:   1697    1781    1865    2033    2117    2283 
   Size:         84      84     168      84     166

  Fragments arranged by size: 

                 427     381     301     168     168     168     166      84
                  84      84      84      84      84
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     130     228    2097    2283 
   Size:        130      98    1869     186

  Fragments arranged by size: 

                1869     186     130      98
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     742     772    2283 
   Size:        742      30    1511

  Fragments arranged by size: 

                1511     742      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     395    2283 
   Size:        395    1888
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     589    2283 
   Size:        589    1694
 
BsiHKAI G_wGCw'C

Cuts at:      0     218    1311    1395    1901    2283 
   Size:        218    1093      84     506     382

  Fragments arranged by size: 

                1093     506     382     218      84
 
BsmI GAATG_Cn'

Cuts at:      0     969    1253    1279    1882    2283 
   Size:        969     284      26     603     401

  Fragments arranged by size: 

                 969     603     401     284      26
 
Bsp1286I G_dGCh'C

Cuts at:      0     218    1311    1395    1901    2283 
   Size:        218    1093      84     506     382

  Fragments arranged by size: 

                1093     506     382     218      84
 
BsrDI GCAATG_nn'

Cuts at:      0    1536    1620    2283 
   Size:       1536      84     663

  Fragments arranged by size: 

                1536     663      84
 
BsrGI T'GTAC_A

Cuts at:      0     638     984    1068    2283 
   Size:        638     346      84    1215

  Fragments arranged by size: 

                1215     638     346      84
 
BstEII G'GTnAC_C

Cuts at:      0     492    2283 
   Size:        492    1791
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1285    2283 
   Size:       1285     998
 
BtgI C'CryG_G

Cuts at:      0     673    2283 
   Size:        673    1610
 
BtsI GCAGTG_nn'

Cuts at:      0     365    1872    2283 
   Size:        365    1507     411

  Fragments arranged by size: 

                1507     411     365
 
ClaI AT'CG_AT

Cuts at:      0     505    2283 
   Size:        505    1778
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1518    1553    1938    1973    2283 
   Size:       1518      35     385      35     310

  Fragments arranged by size: 

                1518     385     310      35      35
 
DraI TTT'AAA

Cuts at:      0    1944    2283 
   Size:       1944     339
 
EarI CTCTTCn'nnn_

Cuts at:      0     389    2283 
   Size:        389    1894
 
EcoICRI GAG'CTC

Cuts at:      0     216    2283 
   Size:        216    2067
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     301     728    1109    1277    1281    1361    1365    1445 
   Size:        301     427     381     168       4      80       4      80

Cuts at:   1445    1449    1529    1555    1697    1781    1785    1865    2033 
   Size:          4      80      26     142      84       4      80     168

Cuts at:   2033    2117    2283 
   Size:         84     166

  Fragments arranged by size: 

                 427     381     301     168     168     166     142      84
                  84      80      80      80      80      26       4       4
                   4       4
 
EcoO109I rG'GnC_Cy

Cuts at:      0     703    2283 
   Size:        703    1580
 
EcoRI G'AATT_C

Cuts at:      0    1331    1667    2171    2283 
   Size:       1331     336     504     112

  Fragments arranged by size: 

                1331     504     336     112
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     946    2283 
   Size:        946    1337
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     742     774    1476    1508    1536    1568    1800    1832 
   Size:        742      32     702      32      28      32     232      32

Cuts at:   1832    2283 
   Size:        451

  Fragments arranged by size: 

                 742     702     451     232      32      32      32      32
                  28
 
HincII GTy'rAC

Cuts at:      0     145     205    2283 
   Size:        145      60    2078

  Fragments arranged by size: 

                2078     145      60
 
HindIII A'AGCT_T

Cuts at:      0    2048    2283 
   Size:       2048     235
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1603    2283 
   Size:       1603     680
 
MslI CAynn'nnrTG

Cuts at:      0     866    1272    1283    1440    1608    1860    2252    2264 
   Size:        866     406      11     157     168     252     392      12

Cuts at:   2264    2283 
   Size:         19

  Fragments arranged by size: 

                 866     406     392     252     168     157      19      12
                  11
 
NcoI C'CATG_G

Cuts at:      0     673    2283 
   Size:        673    1610
 
NdeI CA'TA_TG

Cuts at:      0     807    1059    1269    2283 
   Size:        807     252     210    1014

  Fragments arranged by size: 

                1014     807     252     210
 
NsiI A_TGCA'T

Cuts at:      0    2199    2283 
   Size:       2199      84
 
NspI r_CATG'y

Cuts at:      0     463     740    2153    2201    2283 
   Size:        463     277    1413      48      82

  Fragments arranged by size: 

                1413     463     277      82      48
 
PfoI T'CCnGG_A

Cuts at:      0    1086    2283 
   Size:       1086    1197
 
PshAI GACnn'nnGTC

Cuts at:      0    1382    2283 
   Size:       1382     901
 
PsiI TTA'TAA

Cuts at:      0    1691    2283 
   Size:       1691     592
 
SacI G_AGCT'C

Cuts at:      0     218    2283 
   Size:        218    2065
 
SalI G'TCGA_C

Cuts at:      0     143    2283 
   Size:        143    2140
 
ScaI AGT'ACT

Cuts at:      0    1644    2283 
   Size:       1644     639
 
SfcI C'TryA_G

Cuts at:      0     527     917    2283 
   Size:        527     390    1366

  Fragments arranged by size: 

                1366     527     390
 
SmlI C'TyrA_G

Cuts at:      0       9     223    2283 
   Size:          9     214    2060

  Fragments arranged by size: 

                2060     214       9
 
StyI C'CwwG_G

Cuts at:      0     673    2283 
   Size:        673    1610
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     553    2239    2283 
   Size:        553    1686      44

  Fragments arranged by size: 

                1686     553      44
 
TatI w'GTAC_w

Cuts at:      0     638     739     984    1068    1558    1642    2230    2283 
   Size:        638     101     245      84     490      84     588      53

  Fragments arranged by size: 

                 638     588     490     245     101      84      84      53
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     620     652    2283 
   Size:        620      32    1631

  Fragments arranged by size: 

                1631     620      32
 
XbaI T'CTAG_A

Cuts at:      0     390    2283 
   Size:        390    1893
 
XmnI GAAnn'nnTTC

Cuts at:      0    1378    1546    1671    1798    1882    2050    2283 
   Size:       1378     168     125     127      84     168     233

  Fragments arranged by size: 

                1378     233     168     168     127     125      84
 

 Enzymes that do cut:

     AccI     AcuI    AflII   AflIII     AhdI     AleI     AloI    ApaLI
     ApoI     AseI     BaeI     BanI    BanII     BbsI     BcgI    BciVI
    BfrBI Bme1580I     BmrI     BplI     BpmI     BsaI    BsaXI    BseRI
     BsgI  BsiHKAI     BsmI Bsp1286I    BsrDI    BsrGI   BstEII    BstXI
     BtgI     BtsI     ClaI    CspCI     DraI     EarI  EcoICRI  Eco57MI
 EcoO109I    EcoRI     FalI    Hin4I   HincII  HindIII     MmeI     MslI
     NcoI     NdeI     NsiI     NspI     PfoI    PshAI     PsiI     SacI
     SalI     ScaI     SfcI     SmlI     StyI    TaqII     TatI     TstI
     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI     AgeI     AlfI    AlwNI
     ApaI     AscI    AsiSI     AvaI    AvrII    BamHI     BbeI    BbvCI
     BclI     BglI    BglII     BlpI    BmgBI     BmtI   Bpu10I    BpuEI
    BsaAI    BsaBI    BsaHI    BsaWI    BseYI    BsiEI    BsiWI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrFI   BssHII    BssSI   BstAPI
    BstBI    BstYI  BstZ17I   Bsu36I    BtgZI   DraIII     DrdI     EaeI
     EagI     EciI    EcoNI    EcoRV     FseI     FspI    FspAI    HaeII
     HpaI     KasI     KpnI     MfeI     MluI     MscI   MspA1I     NaeI
     NarI   NgoMIV     NheI     NotI     NruI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PpuMI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII    SacII    SanDI     SapI     SbfI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI  Tth111I     XcmI     XhoI
     XmaI     ZraI
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