Restriction map for pF1KB4786
 (Linear) (Six-base) MAPSORT of: KIBB4786.seq  Check: 6924  from: 1  to: 4392



KIBB4786.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:37  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     124    3781    4392 
   Size:        124    3657     611

  Fragments arranged by size: 

                3657     611     124
 
AatII G_ACGT'C

Cuts at:      0    2754    4392 
   Size:       2754    1638
 
Acc65I G'GTAC_C

Cuts at:      0     137    4392 
   Size:        137    4255
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     623    1109    2161    3576    4392 
   Size:        623     486    1052    1415     816

  Fragments arranged by size: 

                1415    1052     816     623     486
 
AfeI AGC'GCT

Cuts at:      0    2601    3579    4392 
   Size:       2601     978     813

  Fragments arranged by size: 

                2601     978     813
 
AflIII A'CryG_T

Cuts at:      0     152    4392 
   Size:        152    4240
 
AleI CACnn'nnGTG

Cuts at:      0    3632    4392 
   Size:       3632     760
 
ApaI G_GGCC'C

Cuts at:      0     907     998    1079    1267    3004    3491    4392 
   Size:        907      91      81     188    1737     487     901

  Fragments arranged by size: 

                1737     907     901     487     188      91      81
 
ApaLI G'TGCA_C

Cuts at:      0     581    4392 
   Size:        581    3811
 
ApoI r'AATT_y

Cuts at:      0     521    4357    4392 
   Size:        521    3836      35

  Fragments arranged by size: 

                3836     521      35
 
AvaI C'yCGr_G

Cuts at:      0     163     259     356     656     755    1079    1241    1412 
   Size:        163      96      97     300      99     324     162     171

Cuts at:   1412    2087    2845    3419    3685    4392 
   Size:        675     758     574     266     707

  Fragments arranged by size: 

                 758     707     675     574     324     300     266     171
                 163     162      99      97      96
 
BamHI G'GATC_C

Cuts at:      0    2803    4392 
   Size:       2803    1589
 
BanI G'GyrC_C

Cuts at:      0     137     247     823    1192    1237    1606    1714    2002 
   Size:        137     110     576     369      45     369     108     288

Cuts at:   2002    2137    2164    2326    2389    3055    3091    3154    3244 
   Size:        135      27     162      63     666      36      63      90

Cuts at:   3244    4342    4392 
   Size:       1098      50

  Fragments arranged by size: 

                1098     666     576     369     369     288     162     137
                 135     110     108      90      63      63      50      45
                  36      27
 
BanII G_rGCy'C

Cuts at:      0     170     891     907     945     998    1079    1267    1413 
   Size:        170     721      16      38      53      81     188     146

Cuts at:   1413    1775    1854    2151    2385    3004    3051    3491    3742 
   Size:        362      79     297     234     619      47     440     251

Cuts at:   3742    4027    4177    4392 
   Size:        285     150     215

  Fragments arranged by size: 

                 721     619     440     362     297     285     251     234
                 215     188     170     150     146      81      79      53
                  47      38      16
 
BbeI G_GCGC'C

Cuts at:      0     251    2006    2168    3095    3248    4392 
   Size:        251    1755     162     927     153    1144

  Fragments arranged by size: 

                1755    1144     927     251     162     153
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     104    2203    2914    3858    4392 
   Size:        104    2099     711     944     534

  Fragments arranged by size: 

                2099     944     711     534     104
 
BglI GCCn_nnn'nGGC

Cuts at:      0     290     359     386     395     741     911    1127    1271 
   Size:        290      69      27       9     346     170     216     144

Cuts at:   1271    1550    1910    2432    2792    3170    3179    3242    3368 
   Size:        279     360     522     360     378       9      63     126

Cuts at:   3368    3746    4042    4392 
   Size:        378     296     350

  Fragments arranged by size: 

                 522     378     378     360     360     350     346     296
                 290     279     216     170     144     126      69      63
                  27       9       9
 
Bme1580I G_kGCm'C

Cuts at:      0     585     907     998    1079    1242    1267    1719    2142 
   Size:        585     322      91      81     163      25     452     423

Cuts at:   2142    2331    2394    3004    3159    3491    4347    4392 
   Size:        189      63     610     155     332     856      45

  Fragments arranged by size: 

                 856     610     585     452     423     332     322     189
                 163     155      91      81      63      45      25
 
BmgBI CAC'GTC

Cuts at:      0     153     216    3040    4392 
   Size:        153      63    2824    1352

  Fragments arranged by size: 

                2824    1352     153      63
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    3908    4392 
   Size:       3908     484
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     653     959    1409    1787    1805    1868    1937    2012 
   Size:        653     306     450     378      18      63      69      75

Cuts at:   2012    2039    2165    2399    2597    2984    3331    3416    4153 
   Size:         27     126     234     198     387     347      85     737

Cuts at:   4153    4392 
   Size:        239

  Fragments arranged by size: 

                 737     653     450     387     378     347     306     239
                 234     198     126      85      75      69      63      27
                  18
 
Bpu10I CC'TnA_GC

Cuts at:      0    1052    1340    1853    2123    3712    4392 
   Size:       1052     288     513     270    1589     680

  Fragments arranged by size: 

                1589    1052     680     513     288     270
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2837    3599    3688    3769    4135    4392 
   Size:       2837     762      89      81     366     257

  Fragments arranged by size: 

                2837     762     366     257      89      81
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     223     568    1936    1988    2393    3106    3475    3883 
   Size:        223     345    1368      52     405     713     369     408

Cuts at:   3883    4392 
   Size:        509

  Fragments arranged by size: 

                1368     713     509     408     405     369     345     223
                  52
 
BsaBI GATnn'nnATC

Cuts at:      0    4189    4392 
   Size:       4189     203
 
BsaHI Gr'CG_yC

Cuts at:      0     248     326    2003    2165    2282    2751    3092    3245 
   Size:        248      78    1677     162     117     469     341     153

Cuts at:   3245    4392 
   Size:       1147

  Fragments arranged by size: 

                1677    1147     469     341     248     162     153     117
                  78
 
BsaWI w'CCGG_w

Cuts at:      0     514    3734    4255    4392 
   Size:        514    3220     521     137

  Fragments arranged by size: 

                3220     521     514     137
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      22    1421    2915    4156    4392 
   Size:         22    1399    1494    1241     236

  Fragments arranged by size: 

                1494    1399    1241     236      22
 
BseYI C'CCAG_C

Cuts at:      0     482    2356    3574    3605    4392 
   Size:        482    1874    1218      31     787

  Fragments arranged by size: 

                1874    1218     787     482      31
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2222    4227    4392 
   Size:       2222    2005     165

  Fragments arranged by size: 

                2222    2005     165
 
BsiEI CG_ry'CG

Cuts at:      0    3507    4392 
   Size:       3507     885
 
BsiHKAI G_wGCw'C

Cuts at:      0     585     756     891     963    1206    1224    1314    1413 
   Size:        585     171     135      72     243      18      90      99

Cuts at:   1413    1854    2106    2151    2385    2574    2592    2835    3141 
   Size:        441     252      45     234     189      18     243     306

Cuts at:   3141    3150    3447    4392 
   Size:          9     297     945

  Fragments arranged by size: 

                 945     585     441     306     297     252     243     243
                 234     189     171     135      99      90      72      45
                  18      18       9
 
BsmI GAATG_Cn'

Cuts at:      0    2189    4392 
   Size:       2189    2203
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     143    3071    4392 
   Size:        143    2928    1321

  Fragments arranged by size: 

                2928    1321     143
 
Bsp1286I G_dGCh'C

Cuts at:      0     170     585     756     891     907     945     963     998 
   Size:        170     415     171     135      16      38      18      35

Cuts at:    998    1079    1206    1224    1242    1267    1314    1413    1719 
   Size:         81     127      18      18      25      47      99     306

Cuts at:   1719    1775    1854    2106    2142    2151    2331    2385    2394 
   Size:         56      79     252      36       9     180      54       9

Cuts at:   2394    2574    2592    2835    3004    3051    3141    3150    3159 
   Size:        180      18     243     169      47      90       9       9

Cuts at:   3159    3447    3491    3742    4027    4177    4347    4392 
   Size:        288      44     251     285     150     170      45

  Fragments arranged by size: 

                 415     306     288     285     252     251     243     180
                 180     171     170     170     169     150     135     127
                  99      90      81      79      56      54      47      47
                  45      44      38      36      35      25      18      18
                  18      18      16       9       9       9       9
 
BspEI T'CCGG_A

Cuts at:      0    3734    4392 
   Size:       3734     658
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     124    3781    3901    4392 
   Size:        124    3657     120     491

  Fragments arranged by size: 

                3657     491     124     120
 
BsrFI r'CCGG_y

Cuts at:      0     322    1009    1675    2296    3115    3223    4392 
   Size:        322     687     666     621     819     108    1169

  Fragments arranged by size: 

                1169     819     687     666     621     322     108
 
BssSI C'ACGA_G

Cuts at:      0     557     962    2384    4245    4392 
   Size:        557     405    1422    1861     147

  Fragments arranged by size: 

                1861    1422     557     405     147
 
BstEII G'GTnAC_C

Cuts at:      0    3100    3325    4392 
   Size:       3100     225    1067

  Fragments arranged by size: 

                3100    1067     225
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    4141    4392 
   Size:       4141     251
 
BstYI r'GATC_y

Cuts at:      0     176    1561    2803    4190    4392 
   Size:        176    1385    1242    1387     202

  Fragments arranged by size: 

                1387    1385    1242     202     176
 
Bsu36I CC'TnA_GG

Cuts at:      0     133     746     854    2222    4392 
   Size:        133     613     108    1368    2170

  Fragments arranged by size: 

                2170    1368     613     133     108
 
BtgI C'CryG_G

Cuts at:      0     539     647     909    1476    1719    2286    2403    2727 
   Size:        539     108     262     567     243     567     117     324

Cuts at:   2727    2996    3105    3249    3276    3330    4392 
   Size:        269     109     144      27      54    1062

  Fragments arranged by size: 

                1062     567     567     539     324     269     262     243
                 144     117     109     108      54      27
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     224    2522    4392 
   Size:        224    2298    1870

  Fragments arranged by size: 

                2298    1870     224
 
BtsI GCAGTG_nn'

Cuts at:      0    4104    4392 
   Size:       4104     288
 
ClaI AT'CG_AT

Cuts at:      0    4319    4392 
   Size:       4319      73
 
DraIII CAC_nnn'GTG

Cuts at:      0    1714    4392 
   Size:       1714    2678
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    3467    3557    4392 
   Size:       3467      90     835

  Fragments arranged by size: 

                3467     835      90
 
EaeI y'GGCC_r

Cuts at:      0      41      63     387    2460    2640    3636    4017    4043 
   Size:         41      22     324    2073     180     996     381      26

Cuts at:   4043    4392 
   Size:        349

  Fragments arranged by size: 

                2073     996     381     349     324     180      41      26
                  22
 
EarI CTCTTCn'nnn_

Cuts at:      0    3802    4392 
   Size:       3802     590
 
EcoICRI GAG'CTC

Cuts at:      0     889    1411    1852    2149    2383    4392 
   Size:        889     522     441     297     234    2009

  Fragments arranged by size: 

                2009     889     522     441     297     234
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     623     653     959    1109    1409    1787    1805    1868 
   Size:        623      30     306     150     300     378      18      63

Cuts at:   1868    1937    2012    2039    2161    2165    2399    2597    2984 
   Size:         69      75      27     122       4     234     198     387

Cuts at:   2984    3331    3416    3576    4153    4392 
   Size:        347      85     160     577     239

  Fragments arranged by size: 

                 623     577     387     378     347     306     300     239
                 234     198     160     150     122      85      75      69
                  63      30      27      18       4
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    4153    4392 
   Size:       4153     239
 
EcoO109I rG'GnC_Cy

Cuts at:      0     334     370     379     544     607     643     652     661 
   Size:        334      36       9     165      63      36       9       9

Cuts at:    661     670     904     995    1075    1076    1093    1246    1263 
   Size:          9     234      91      80       1      17     153      17

Cuts at:   1263    1264    1372    1516    1858    1903    2065    2218    2722 
   Size:          1     108     144     342      45     162     153     504

Cuts at:   2722    2956    3001    3271    3352    3388    3424    3488    4158 
   Size:        234      45     270      81      36      36      64     670

Cuts at:   4158    4392 
   Size:        234

  Fragments arranged by size: 

                 670     504     342     334     270     234     234     234
                 165     162     153     153     144     108      91      81
                  80      64      63      45      45      36      36      36
                  36      17      17       9       9       9       9       1
                   1
 
EcoRI G'AATT_C

Cuts at:      0    4357    4392 
   Size:       4357      35
 
HaeII r_GCGC'y

Cuts at:      0     251    1817    2006    2168    2603    3095    3248    3581 
   Size:        251    1566     189     162     435     492     153     333

Cuts at:   3581    4392 
   Size:        811

  Fragments arranged by size: 

                1566     811     492     435     333     251     189     162
                 153
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     383     415     493     525    2788    2820    3177    3209 
   Size:        383      32      78      32    2263      32     357      32

Cuts at:   3209    4392 
   Size:       1183

  Fragments arranged by size: 

                2263    1183     383     357      78      32      32      32
                  32
 
KasI G'GCGC_C

Cuts at:      0     247    2002    2164    3091    3244    4392 
   Size:        247    1755     162     927     153    1148

  Fragments arranged by size: 

                1755    1148     927     247     162     153
 
KpnI G_GTAC'C

Cuts at:      0     141    4392 
   Size:        141    4251
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    4052    4202    4392 
   Size:       4052     150     190

  Fragments arranged by size: 

                4052     190     150
 
MscI TGG'CCA

Cuts at:      0    4045    4392 
   Size:       4045     347
 
MslI CAynn'nnrTG

Cuts at:      0    3632    4321    4392 
   Size:       3632     689      71

  Fragments arranged by size: 

                3632     689      71
 
MspA1I CmG'CkG

Cuts at:      0     486     775    1018    1298    1522    1882    2206    2360 
   Size:        486     289     243     280     224     360     324     154

Cuts at:   2360    2539    3124    4053    4392 
   Size:        179     585     929     339

  Fragments arranged by size: 

                 929     585     486     360     339     324     289     280
                 243     224     179     154
 
NarI GG'CG_CC

Cuts at:      0     248    2003    2165    3092    3245    4392 
   Size:        248    1755     162     927     153    1147

  Fragments arranged by size: 

                1755    1147     927     248     162     153
 
NcoI C'CATG_G

Cuts at:      0     539     647    2996    4392 
   Size:        539     108    2349    1396

  Fragments arranged by size: 

                2349    1396     539     108
 
NspI r_CATG'y

Cuts at:      0    3970    4392 
   Size:       3970     422
 
PasI CC'CwG_GG

Cuts at:      0    1098    1260    4392 
   Size:       1098     162    3132

  Fragments arranged by size: 

                3132    1098     162
 
PfoI T'CCnGG_A

Cuts at:      0     420     447    1800    1989    2574    3546    4392 
   Size:        420      27    1353     189     585     972     846

  Fragments arranged by size: 

                1353     972     846     585     420     189      27
 
PpuMI rG'GwC_Cy

Cuts at:      0     334     370     544     607     643     652     661     670 
   Size:        334      36     174      63      36       9       9       9

Cuts at:    670    1093    1372    1516    1858    2065    2722    2956    3352 
   Size:        423     279     144     342     207     657     234     396

Cuts at:   3352    3388    3424    4392 
   Size:         36      36     968

  Fragments arranged by size: 

                 968     657     423     396     342     334     279     234
                 207     174     144      63      36      36      36      36
                   9       9       9
 
PspOMI G'GGCC_C

Cuts at:      0     903     994    1075    1263    3000    3487    4392 
   Size:        903      91      81     188    1737     487     905

  Fragments arranged by size: 

                1737     905     903     487     188      91      81
 
PspXI vC'TCGA_Gb

Cuts at:      0     755    1412    3685    4392 
   Size:        755     657    2273     707

  Fragments arranged by size: 

                2273     755     707     657
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    4084    4116    4392 
   Size:       4084      32     276

  Fragments arranged by size: 

                4084     276      32
 
PvuII CAG'CTG

Cuts at:      0     486     775    2206    4053    4392 
   Size:        486     289    1431    1847     339

  Fragments arranged by size: 

                1847    1431     486     339     289
 
SacI G_AGCT'C

Cuts at:      0     891    1413    1854    2151    2385    4392 
   Size:        891     522     441     297     234    2007

  Fragments arranged by size: 

                2007     891     522     441     297     234
 
SanDI GG'GwC_CC

Cuts at:      0     334     370     544     652    1516    1858    3352    4392 
   Size:        334      36     174     108     864     342    1494    1040

  Fragments arranged by size: 

                1494    1040     864     342     334     174     108      36
            
 
ScaI AGT'ACT

Cuts at:      0    3820    4392 
   Size:       3820     572
 
SexAI A'CCwGG_T

Cuts at:      0     585     711    1305    1467    1710    1971    2466    2484 
   Size:        585     126     594     162     243     261     495      18

Cuts at:   2484    4392 
   Size:       1908

  Fragments arranged by size: 

                1908     594     585     495     261     243     162     126
                  18
 
SfcI C'TryA_G

Cuts at:      0    4221    4392 
   Size:       4221     171
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     359     386     741     911    1127    1271    1550    3170 
   Size:        359      27     355     170     216     144     279    1620

Cuts at:   3170    4392 
   Size:       1222

  Fragments arranged by size: 

                1620    1222     359     355     279     216     170     144
                  27
 
SfoI GGC'GCC

Cuts at:      0     249    2004    2166    3093    3246    4392 
   Size:        249    1755     162     927     153    1146

  Fragments arranged by size: 

                1755    1146     927     249     162     153
 
SgrAI Cr'CCGG_yG

Cuts at:      0     322    4392 
   Size:        322    4070
 
SmaI CCC'GGG

Cuts at:      0    2847    4392 
   Size:       2847    1545
 
SmlI C'TyrA_G

Cuts at:      0     755    1412    2852    3614    3685    3703    3784    4150 
   Size:        755     657    1440     762      71      18      81     366

Cuts at:   4150    4392 
   Size:        242

  Fragments arranged by size: 

                1440     762     755     657     366     242      81      71
                  18
 
StuI AGG'CCT

Cuts at:      0    4084    4392 
   Size:       4084     308
 
StyI C'CwwG_G

Cuts at:      0     338     364     539     594     603     647     827    1071 
   Size:        338      26     175      55       9      44     180     244

Cuts at:   1071    1286    1511    1610    1998    2996    3095    3267    3837 
   Size:        215     225      99     388     998      99     172     570

Cuts at:   3837    3953    4331    4392 
   Size:        116     378      61

  Fragments arranged by size: 

                 998     570     388     378     338     244     225     215
                 180     175     172     116      99      99      61      55
                  44      26       9
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     161     318    1810    2638    4006    4392 
   Size:        161     157    1492     828    1368     386

  Fragments arranged by size: 

                1492    1368     828     386     161     157
 
TatI w'GTAC_w

Cuts at:      0    3818    4392 
   Size:       3818     574
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     512     544    4392 
   Size:        512      32    3848

  Fragments arranged by size: 

                3848     512      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     490     610    1105    1933    2167    3274    3355    4009 
   Size:        490     120     495     828     234    1107      81     654

Cuts at:   4009    4320    4392 
   Size:        311      72

  Fragments arranged by size: 

                1107     828     654     495     490     311     234     120
                  81      72
 
XhoI C'TCGA_G

Cuts at:      0     755    1412    3685    4392 
   Size:        755     657    2273     707

  Fragments arranged by size: 

                2273     755     707     657
 
XmaI C'CCGG_G

Cuts at:      0    2845    4392 
   Size:       2845    1547
 
ZraI GAC'GTC

Cuts at:      0    2752    4392 
   Size:       2752    1640
 

 Enzymes that do cut:

     AarI    AatII   Acc65I     AcuI     AfeI   AflIII     AleI     ApaI
    ApaLI     ApoI     AvaI    BamHI     BanI    BanII     BbeI     BbsI
     BglI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I    BpuEI     BsaI
    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspEI    BspMI    BsrFI    BssSI   BstEII
    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI   DraIII
     DrdI     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    HaeII    Hin4I     KasI     KpnI     MmeI     MscI     MslI   MspA1I
     NarI     NcoI     NspI     PasI     PfoI    PpuMI   PspOMI    PspXI
     PsrI    PvuII     SacI    SanDI     ScaI    SexAI     SfcI     SfiI
     SfoI    SgrAI     SmaI     SmlI     StuI     StyI    TaqII     TatI
     TstI     XcmI     XhoI     XmaI     ZraI

 Enzymes that do not cut: 

     AccI     AclI    AflII     AgeI     AhdI     AlfI     AloI    AlwNI
     AscI     AseI    AsiSI    AvrII     BaeI    BbvCI     BcgI    BciVI
     BclI    BfrBI    BglII     BlpI     BmtI     BplI    BsaAI    BsaXI
    BsiWI    BspHI    BsrBI    BsrDI    BsrGI   BssHII   BstAPI    BstBI
  BstZ17I    CspCI     DraI     EagI     EciI    EcoRV     FalI     FseI
     FspI    FspAI   HincII  HindIII     HpaI     MfeI     MluI     NaeI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PciI
    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI     PstI     PvuI
    RsrII    SacII     SalI     SapI     SbfI     SgfI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI  Tth111I     XbaI     XmnI
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