Restriction map for pF1KB4336
##################################
# NO CUT ENZYMES (   97 enzymes) #
##################################

AatII       AccIII      AclI        AcsI        AgeI        
Alw44I      Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     AviII       AvrII       
BamHI       BcgI        BciVI       BclI        BglII       
BlnI        BlpI        Bpu1102I    BsaBI       BseAI       
BsiEI       BsiWI       BsmBI       Bsp1407I    BspDI       
BspEI       BspLU11I    BspMI       BsrDI       BsrGI       
BssHII      Bst1107I    BstBI       BstEII      BstPI       
BstZ17I     CelII       ClaI        CpoI        DraI        
DrdI        EagI        EclXI       Eco47III    Eco52I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HpaI        KspI        MamI        
MfeI        MroI        MunI        NdeI        NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflMI       PinAI       PmeI        
PshBI       Psp1406I    PvuI        RsrII       SacII       
SapI        SbfI        SexAI       SfiI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        Van91I      XbaI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

Acc65I       3022  GGTACC               G^GTACC
AflII         742  CTTAAG               C^TTAAG
AocI         1987  CCTCAGG              CC^T[ACGT]AGG
Asp718I      3022  GGTACC               G^GTACC
BalI         2978  TGGCCA               TGG^CCA
BbeI         1260  GGCGCC               GGCGC^C
BbrPI         253  CACGTG               CAC^GTG
BfrI          742  CTTAAG               C^TTAAG
BsaWI        1967  TCCGGT               [AT]^CCGG[AT]
BsmI         1069  GCATTC               GAATGC(1/-1)
BspHI         336  TCATGA               T^CATGA
Bsu36I       1987  CCTCAGG              CC^T[ACGT]AGG
Eco81I       1987  CCTCAGG              CC^T[ACGT]AGG
EcoNI        1375  CCTGAGACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
KasI         1260  GGCGCC               G^GCGCC
KpnI         3022  GGTACC               GGTAC^C
MluI           83  ACGCGT               A^CGCGT
MluNI        2978  TGGCCA               TGG^CCA
MscI         2978  TGGCCA               TGG^CCA
NarI         1260  GGCGCC               GG^CGCC
PmaCI         253  CACGTG               CAC^GTG
PmlI          253  CACGTG               CAC^GTG
PshAI        2892  GACTGTTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI          336  TCATGA               T^CATGA
SacI         1050  GAGCTC               GAGCT^C
SalI          434  GTCGAC               G^TCGAC
SfoI         1260  GGCGCC               GGC^GCC
StuI         1383  AGGCCT               AGG^CCT
Tsp509I       287  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AccII          84  CGCG                 CG^CG
             2181  CGCG                
AcyI         1260  GGCGCC               G[GA]^CG[CT]C
             2146  GGCGTC              
AflIII         83  ACGCGT               A^C[GA][CT]GT
              252  ACACGT              
AhdI         1276  GACTATAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3158  GACCTCCAGTC         
AspEI        1276  GACTATAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3158  GACCTCCAGTC         
AspI          505  GACAACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              814  GACAAGGTC           
BfaI         1642  CTAG                 C^TAG
             3079  CTAG                
BsaAI         253  CACGTG               [CT]AC^GT[GA]
              328  TACGTG              
BsaHI        1260  GGCGCC               G[GA]^CG[CT]C
             2146  GGCGTC              
BsrBI        1776  CCGCTC               CCGCTC(-3/-3)
             2242  GAGCGG              
BssSI         700  CACGAG               CACGAG(-5/-1)
              850  CACGAG              
BstUI          84  CGCG                 CG^CG
             2181  CGCG                
BstYI        2216  AGATCC               [GA]^GATC[CT]
             2918  AGATCC              
Eam1105I     1276  GACTATAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3158  GACCTCCAGTC         
HgaI         2147  GCGTC                GACGC(5/10)
             3046  GCGTC               
Hin1I        1260  GGCGCC               G[GA]^CG[CT]C
             2146  GGCGTC              
HincII        434  GTCGAC               GT[CT]^[GA]AC
             2149  GTCAAC              
HindII        434  GTCGAC               GT[CT]^[GA]AC
             2149  GTCAAC              
MaeI         1642  CTAG                 C^TAG
             3079  CTAG                
MflI         2216  AGATCC               [GA]^GATC[CT]
             2918  AGATCC              
MseI          743  TTAA                 T^TAA
             1904  TTAA                
MvnI           84  CGCG                 CG^CG
             2181  CGCG                
NaeI         2143  GCCGGC               GCC^GGC
             2353  GCCGGC              
NgoMIV       2143  GCCGGC               G^CCGGC
             2353  GCCGGC              
PflFI         505  GACAACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              814  GACAAGGTC           
ScaI          515  AGTACT               AGT^ACT
             1103  AGTACT              
SmaI         1256  CCCGGG               CCC^GGG
             2608  CCCGGG              
TfiI          163  GAATC                G^A[AT]TC
             2253  GATTC               
Tru9I         743  TTAA                 T^TAA
             1904  TTAA                
Tsp45I        451  GTCAC                ^GT[GC]AC
             1294  GTCAC               
Tth111I       505  GACAACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              814  GACAAGGTC           
XhoII        2216  AGATCC               [GA]^GATC[CT]
             2918  AGATCC              
XmaCI        1256  CCCGGG               C^CCGGG
             2608  CCCGGG              
XmaI         1256  CCCGGG               C^CCGGG
             2608  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   98 enzymes) #
#########################################

AccI        AciI        AfaI        AluI        AlwI        
AlwNI       ApaI        AspHI       AvaI        AvaII       
BanI        BanII       BbsI        BbvCI       BbvI        
BcnI        BglI        BmyI        BpmI        BpuAI       
BsaI        BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BsrFI       BsrI        BssKI       BstAPI      
BstF5I      BstNI       BstXI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DraIII      DsaI        EaeI        EarI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        HinP1I      
HindIII     HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeII       MaeIII      MboI        MboII       
MnlI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NcoI        NdeII       NlaIII      
NlaIV       PleI        PpuMI       PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StyI        TaqI        TseI        
TspRI       TthHB8I     XcmI        
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