Restriction map for pF1KB4336
 (Linear) (Six-base) MAPSORT of: KIBB4336.seq  Check: 4427  from: 1  to: 3279



KIBB4336.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:37  ..

AccI GT'mk_AC

Cuts at:      0      68     435    2900    3279 
   Size:         68     367    2465     379

  Fragments arranged by size: 

                2465     379     367      68
 
Acc65I G'GTAC_C

Cuts at:      0    3022    3279 
   Size:       3022     257
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     525    1115    1408    2188    2803    3279 
   Size:        525     590     293     780     615     476

  Fragments arranged by size: 

                 780     615     590     525     476     293
 
AflII C'TTAA_G

Cuts at:      0     742    3279 
   Size:        742    2537
 
AflIII A'CryG_T

Cuts at:      0      83     252    3279 
   Size:         83     169    3027

  Fragments arranged by size: 

                3027     169      83
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1281    3163    3279 
   Size:       1281    1882     116

  Fragments arranged by size: 

                1882    1281     116
 
AleI CACnn'nnGTG

Cuts at:      0    1354    1831    3279 
   Size:       1354     477    1448

  Fragments arranged by size: 

                1448    1354     477
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2546    3279 
   Size:       2546     733
 
AlwNI CAG_nnn'CTG

Cuts at:      0    2053    2118    2205    2220    2859    2946    3049    3127 
   Size:       2053      65      87      15     639      87     103      78

Cuts at:   3127    3279 
   Size:        152

  Fragments arranged by size: 

                2053     639     152     103      87      87      78      65
                  15
 
ApaI G_GGCC'C

Cuts at:      0    1022    1214    1257    1477    1745    2333    2562    2760 
   Size:       1022     192      43     220     268     588     229     198

Cuts at:   2760    3279 
   Size:        519

  Fragments arranged by size: 

                1022     588     519     268     229     220     198     192
                  43
 
AvaI C'yCGr_G

Cuts at:      0     277     399    1156    1256    2460    2608    3279 
   Size:        277     122     757     100    1204     148     671

  Fragments arranged by size: 

                1204     757     671     277     148     122     100
 
BanI G'GyrC_C

Cuts at:      0     129    1260    1318    1897    2604    3022    3279 
   Size:        129    1131      58     579     707     418     257

  Fragments arranged by size: 

                1131     707     579     418     257     129      58
 
BanII G_rGCy'C

Cuts at:      0     314    1022    1054    1163    1214    1257    1477    1745 
   Size:        314     708      32     109      51      43     220     268

Cuts at:   1745    2333    2389    2530    2562    2696    2760    2965    3078 
   Size:        588      56     141      32     134      64     205     113

Cuts at:   3078    3279 
   Size:        201

  Fragments arranged by size: 

                 708     588     314     268     220     205     201     141
                 134     113     109      64      56      51      43      32
                  32
 
BbeI G_GCGC'C

Cuts at:      0    1264    3279 
   Size:       1264    2015
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     559     899    1188    1994    3279 
   Size:        559     340     289     806    1285

  Fragments arranged by size: 

                1285     806     559     340     289
 
BbvCI CC'TCA_GC

Cuts at:      0    1149    1771    2642    3193    3258    3279 
   Size:       1149     622     871     551      65      21

  Fragments arranged by size: 

                1149     871     622     551      65      21
 
BglI GCCn_nnn'nGGC

Cuts at:      0     466    1507    2750    3082    3279 
   Size:        466    1041    1243     332     197

  Fragments arranged by size: 

                1243    1041     466     332     197
 
Bme1580I G_kGCm'C

Cuts at:      0      83     205     252     267     462    1022    1214    1257 
   Size:         83     122      47      15     195     560     192      43

Cuts at:   1257    1468    1477    1503    1745    2279    2333    2562    2607 
   Size:        211       9      26     242     534      54     229      45

Cuts at:   2607    2760    3279 
   Size:        153     519

  Fragments arranged by size: 

                 560     534     519     242     229     211     195     192
                 153     122      83      54      47      45      43      26
                  15       9
 
BmgBI CAC'GTC

Cuts at:      0     450    1293    3279 
   Size:        450     843    1986

  Fragments arranged by size: 

                1986     843     450
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1478    1490    3279 
   Size:       1478      12    1789

  Fragments arranged by size: 

                1789    1478      12
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     517     614    1102    1224    1565    1648    1713    1972 
   Size:        517      97     488     122     341      83      65     259

Cuts at:   1972    2140    2503    2824    2833    3092    3146    3279 
   Size:        168     363     321       9     259      54     133

  Fragments arranged by size: 

                 517     488     363     341     321     259     259     168
                 133     122      97      83      65      54       9
 
Bpu10I CC'TnA_GC

Cuts at:      0    1149    1245    1771    2221    2642    3193    3258    3279 
   Size:       1149      96     526     450     421     551      65      21

  Fragments arranged by size: 

                1149     551     526     450     421      96      65      21
            
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1641    2391    2760    3279 
   Size:       1641     750     369     519

  Fragments arranged by size: 

                1641     750     519     369
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     825     862    1363    3279 
   Size:        825      37     501    1916

  Fragments arranged by size: 

                1916     825     501      37
 
BsaAI yAC'GTr

Cuts at:      0     255     330    3279 
   Size:        255      75    2949

  Fragments arranged by size: 

                2949     255      75
 
BsaHI Gr'CG_yC

Cuts at:      0    1261    2147    3279 
   Size:       1261     886    1132

  Fragments arranged by size: 

                1261    1132     886
 
BsaWI w'CCGG_w

Cuts at:      0    1967    3279 
   Size:       1967    1312
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     805     835    1087    1090    1117    1120    1663    1693 
   Size:        805      30     252       3      27       3     543      30

Cuts at:   1693    1984    2014    3065    3095    3279 
   Size:        291      30    1051      30     184

  Fragments arranged by size: 

                1051     805     543     291     252     184      30      30
                  30      30      27       3       3
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     385     599     611     614     660     813     816     972 
   Size:        385     214      12       3      46     153       3     156

Cuts at:    972     984     987     996     999    1108    1431    1716    1794 
   Size:         12       3       9       3     109     323     285      78

Cuts at:   1794    1903    2506    3279 
   Size:        109     603     773

  Fragments arranged by size: 

                 773     603     385     323     285     214     156     153
                 109     109      78      46      12      12       9       3
                   3       3       3
 
BseYI C'CCAG_C

Cuts at:      0      47     245     778    1476    1502    1615    2579    2622 
   Size:         47     198     533     698      26     113     964      43

Cuts at:   2622    2956    2966    3279 
   Size:        334      10     313

  Fragments arranged by size: 

                 964     698     533     334     313     198     113      47
                  43      26      10
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     108    2039    2161    2284    3279 
   Size:        108    1931     122     123     995

  Fragments arranged by size: 

                1931     995     123     122     108
 
BsiHKAI G_wGCw'C

Cuts at:      0     302    1054    2377    3279 
   Size:        302     752    1323     902

  Fragments arranged by size: 

                1323     902     752     302
 
BsmI GAATG_Cn'

Cuts at:      0    1069    3279 
   Size:       1069    2210
 
Bsp1286I G_dGCh'C

Cuts at:      0      83     205     252     267     302     314     462    1022 
   Size:         83     122      47      15      35      12     148     560

Cuts at:   1022    1054    1163    1214    1257    1468    1477    1503    1745 
   Size:         32     109      51      43     211       9      26     242

Cuts at:   1745    2279    2333    2377    2389    2530    2562    2607    2696 
   Size:        534      54      44      12     141      32      45      89

Cuts at:   2696    2760    2965    3078    3279 
   Size:         64     205     113     201

  Fragments arranged by size: 

                 560     534     242     211     205     201     148     141
                 122     113     109      89      83      64      54      51
                  47      45      44      43      35      32      32      26
                  15      12      12       9
 
BspHI T'CATG_A

Cuts at:      0     336    3279 
   Size:        336    2943
 
BsrBI CCG'CTC

Cuts at:      0    1778    2244    3279 
   Size:       1778     466    1035

  Fragments arranged by size: 

                1778    1035     466
 
BsrFI r'CCGG_y

Cuts at:      0     653    2143    2353    3279 
   Size:        653    1490     210     926

  Fragments arranged by size: 

                1490     926     653     210
 
BssSI C'ACGA_G

Cuts at:      0     700     850    3279 
   Size:        700     150    2429

  Fragments arranged by size: 

                2429     700     150
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     330    1345    2184    2946    3127    3279 
   Size:        330    1015     839     762     181     152

  Fragments arranged by size: 

                1015     839     762     330     181     152
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1510    1737    1882    2637    2988    3279 
   Size:       1510     227     145     755     351     291

  Fragments arranged by size: 

                1510     755     351     291     227     145
 
BstYI r'GATC_y

Cuts at:      0    2216    2918    3279 
   Size:       2216     702     361

  Fragments arranged by size: 

                2216     702     361
 
Bsu36I CC'TnA_GG

Cuts at:      0    1988    3279 
   Size:       1988    1291
 
BtgI C'CryG_G

Cuts at:      0     305     426     797    1425    1892    2636    2975    3279 
   Size:        305     121     371     628     467     744     339     304

  Fragments arranged by size: 

                 744     628     467     371     339     305     304     121
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      89    3279 
   Size:         89    3190
 
BtsI GCAGTG_nn'

Cuts at:      0     633     806     954    1523    2676    3170    3222    3279 
   Size:        633     173     148     569    1153     494      52      57

  Fragments arranged by size: 

                1153     633     569     494     173     148      57      52
            
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      91     126    3279 
   Size:         91      35    3153

  Fragments arranged by size: 

                3153      91      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     258    1345    2062    3279 
   Size:        258    1087     717    1217

  Fragments arranged by size: 

                1217    1087     717     258
 
EaeI y'GGCC_r

Cuts at:      0      95    2070    2231    2978    3116    3279 
   Size:         95    1975     161     747     138     163

  Fragments arranged by size: 

                1975     747     163     161     138      95
 
EarI CTCTTCn'nnn_

Cuts at:      0     220     712     843     990    1005    1812    2041    3279 
   Size:        220     492     131     147      15     807     229    1238

  Fragments arranged by size: 

                1238     807     492     229     220     147     131      15
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     596     966    3170    3279 
   Size:        596     370    2204     109

  Fragments arranged by size: 

                2204     596     370     109
 
EcoICRI GAG'CTC

Cuts at:      0    1052    3279 
   Size:       1052    2227
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     517     525     614    1102    1115    1224    1408    1565 
   Size:        517       8      89     488      13     109     184     157

Cuts at:   1565    1648    1713    1972    2140    2188    2503    2803    2824 
   Size:         83      65     259     168      48     315     300      21

Cuts at:   2824    2833    3092    3146    3279 
   Size:          9     259      54     133

  Fragments arranged by size: 

                 517     488     315     300     259     259     184     168
                 157     133     109      89      83      65      54      48
                  21      13       9       8
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1379    3279 
   Size:       1379    1900
 
EcoO109I rG'GnC_Cy

Cuts at:      0     139    1018    1019    1307    1473    1671    1741    1742 
   Size:        139     879       1     288     166     198      70       1

Cuts at:   1742    1751    1979    2330    2442    2558    2559    2676    2756 
   Size:          9     228     351     112     116       1     117      80

Cuts at:   2756    2757    3279 
   Size:          1     522

  Fragments arranged by size: 

                 879     522     351     288     228     198     166     139
                 117     116     112      80      70       9       1       1
                   1       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     757    1212    3279 
   Size:        757     455    2067

  Fragments arranged by size: 

                2067     757     455
 
HaeII r_GCGC'y

Cuts at:      0     593    1264    2176    2946    3279 
   Size:        593     671     912     770     333

  Fragments arranged by size: 

                 912     770     671     593     333
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     805     837    1090    1122    1267    1299    1663    1695 
   Size:        805      32     253      32     145      32     364      32

Cuts at:   1695    1971    1984    2003    2016    2434    2466    3149    3181 
   Size:        276      13      19      13     418      32     683      32

Cuts at:   3181    3279 
   Size:         98

  Fragments arranged by size: 

                 805     683     418     364     276     253     145      98
                  32      32      32      32      32      32      19      13
                  13
 
HincII GTy'rAC

Cuts at:      0     436    2151    3279 
   Size:        436    1715    1128

  Fragments arranged by size: 

                1715    1128     436
 
HindIII A'AGCT_T

Cuts at:      0     107     440    2736    3279 
   Size:        107     333    2296     543

  Fragments arranged by size: 

                2296     543     333     107
 
KasI G'GCGC_C

Cuts at:      0    1260    3279 
   Size:       1260    2019
 
KpnI G_GTAC'C

Cuts at:      0    3026    3279 
   Size:       3026     253
 
MluI A'CGCG_T

Cuts at:      0      83    3279 
   Size:         83    3196
 
MscI TGG'CCA

Cuts at:      0    2980    3279 
   Size:       2980     299
 
MslI CAynn'nnrTG

Cuts at:      0    1354    1831    2635    3279 
   Size:       1354     477     804     644

  Fragments arranged by size: 

                1354     804     644     477
 
MspA1I CmG'CkG

Cuts at:      0      63     572    1480    1506    2202    2508    2617    2719 
   Size:         63     509     908      26     696     306     109     102

Cuts at:   2719    2772    2874    2970    3009    3070    3127    3133    3244 
   Size:         53     102      96      39      61      57       6     111

Cuts at:   3244    3279 
   Size:         35

  Fragments arranged by size: 

                 908     696     509     306     111     109     102     102
                  96      63      61      57      53      39      35      26
                   6
 
NaeI GCC'GGC

Cuts at:      0    2145    2355    3279 
   Size:       2145     210     924

  Fragments arranged by size: 

                2145     924     210
 
NarI GG'CG_CC

Cuts at:      0    1261    3279 
   Size:       1261    2018
 
NcoI C'CATG_G

Cuts at:      0     305     426    1425    1892    2636    2975    3279 
   Size:        305     121     999     467     744     339     304

  Fragments arranged by size: 

                 999     744     467     339     305     304     121
 
NgoMIV G'CCGG_C

Cuts at:      0    2143    2353    3279 
   Size:       2143     210     926

  Fragments arranged by size: 

                2143     926     210
 
PasI CC'CwG_GG

Cuts at:      0    1270    1747    2092    2326    3279 
   Size:       1270     477     345     234     953

  Fragments arranged by size: 

                1270     953     477     345     234
 
PfoI T'CCnGG_A

Cuts at:      0    2391    3279 
   Size:       2391     888
 
PmlI CAC'GTG

Cuts at:      0     255    3279 
   Size:        255    3024
 
PpuMI rG'GwC_Cy

Cuts at:      0     139    1671    1751    1979    2442    3279 
   Size:        139    1532      80     228     463     837

  Fragments arranged by size: 

                1532     837     463     228     139      80
 
PshAI GACnn'nnGTC

Cuts at:      0    2896    3279 
   Size:       2896     383
 
PspOMI G'GGCC_C

Cuts at:      0    1018    1210    1253    1473    1741    2329    2558    2756 
   Size:       1018     192      43     220     268     588     229     198

Cuts at:   2756    3279 
   Size:        523

  Fragments arranged by size: 

                1018     588     523     268     229     220     198     192
                  43
 
PstI C_TGCA'G

Cuts at:      0     326    1035    1049    1714    1963    2189    2252    2771 
   Size:        326     709      14     665     249     226      63     519

Cuts at:   2771    2942    2951    3132    3138    3279 
   Size:        171       9     181       6     141

  Fragments arranged by size: 

                 709     665     519     326     249     226     181     171
                 141      63      14       9       6
 
PvuII CAG'CTG

Cuts at:      0    1480    1506    2772    2874    2970    3127    3133    3279 
   Size:       1480      26    1266     102      96     157       6     146

  Fragments arranged by size: 

                1480    1266     157     146     102      96      26       6
            
 
SacI G_AGCT'C

Cuts at:      0    1054    3279 
   Size:       1054    2225
 
SalI G'TCGA_C

Cuts at:      0     434    3279 
   Size:        434    2845
 
SanDI GG'GwC_CC

Cuts at:      0     139    3279 
   Size:        139    3140
 
ScaI AGT'ACT

Cuts at:      0     517    1105    3279 
   Size:        517     588    2174

  Fragments arranged by size: 

                2174     588     517
 
SfcI C'TryA_G

Cuts at:      0     322    1031    1045    1710    1959    2185    2248    2767 
   Size:        322     709      14     665     249     226      63     519

Cuts at:   2767    2938    2947    3128    3134    3279 
   Size:        171       9     181       6     145

  Fragments arranged by size: 

                 709     665     519     322     249     226     181     171
                 145      63      14       9       6
 
SfoI GGC'GCC

Cuts at:      0    1262    3279 
   Size:       1262    2017
 
SmaI CCC'GGG

Cuts at:      0    1258    2610    3279 
   Size:       1258    1352     669

  Fragments arranged by size: 

                1352    1258     669
 
SmlI C'TyrA_G

Cuts at:      0     742    1620    2370    2739    3279 
   Size:        742     878     750     369     540

  Fragments arranged by size: 

                 878     750     742     540     369
 
SrfI GCCC'GGGC

Cuts at:      0    1258    3279 
   Size:       1258    2021
 
StuI AGG'CCT

Cuts at:      0    1385    3279 
   Size:       1385    1894
 
StyI C'CwwG_G

Cuts at:      0     305     426    1166    1425    1442    1892    2636    2697 
   Size:        305     121     740     259      17     450     744      61

Cuts at:   2697    2975    3279 
   Size:        278     304

  Fragments arranged by size: 

                 744     740     450     305     304     278     259     121
                  61      17
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     709    1336    1885    3279 
   Size:        709     627     549    1394

  Fragments arranged by size: 

                1394     709     627     549
 
TatI w'GTAC_w

Cuts at:      0     515    1103    3279 
   Size:        515     588    2176

  Fragments arranged by size: 

                2176     588     515
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    3065    3097    3279 
   Size:       3065      32     182

  Fragments arranged by size: 

                3065     182      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     508     817    3279 
   Size:        508     309    2462

  Fragments arranged by size: 

                2462     508     309
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     112     140    1503    1623    2541    3279 
   Size:        112      28    1363     120     918     738

  Fragments arranged by size: 

                1363     918     738     120     112      28
 
XmaI C'CCGG_G

Cuts at:      0    1256    2608    3279 
   Size:       1256    1352     671

  Fragments arranged by size: 

                1352    1256     671
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI    AflII   AflIII     AhdI     AleI     AlfI
    AlwNI     ApaI     AvaI     BanI    BanII     BbeI     BbsI    BbvCI
     BglI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
     BsmI Bsp1286I    BspHI    BsrBI    BsrFI    BssSI   BstAPI    BstXI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI    CspCI   DraIII     EaeI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI    HaeII
    Hin4I   HincII  HindIII     KasI     KpnI     MluI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     PasI     PfoI     PmlI
    PpuMI    PshAI   PspOMI     PstI    PvuII     SacI     SalI    SanDI
     ScaI     SfcI     SfoI     SmaI     SmlI     SrfI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI     AgeI     AloI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BcgI    BciVI
     BclI    BfrBI    BglII     BlpI     BmtI     BplI    BsaBI    BsiEI
    BsiWI    BsmBI    BspEI    BspMI    BsrDI    BsrGI   BssHII    BstBI
   BstEII  BstZ17I     ClaI     DraI     DrdI     EagI    EcoRI    EcoRV
     FseI     FspI    FspAI     HpaI     MfeI     MmeI     NdeI     NheI
     NotI     NruI     NsiI     NspI     PacI     PciI    PflMI     PmeI
     PpiI     PsiI    PspXI     PsrI     PvuI    RsrII    SacII     SapI
     SbfI    SexAI     SfiI     SgfI    SgrAI    SnaBI     SpeI     SphI
     SspI     SwaI     XbaI     XhoI     XmnI     ZraI
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