Restriction map for pF1KB4275
##################################
# NO CUT ENZYMES (  116 enzymes) #
##################################

AatII       AccII       AclI        AcyI        AflII       
AgeI        AhdI        Aor51HI     AscI        AseI        
AsnI        Asp700I     AspEI       AspI        AviII       
AvrII       BamHI       BbeI        BcgI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BsaHI       
BsaI        BsiEI       BsiWI       BsmAI       BsmBI       
Bsp1407I    BspDI       BspHI       BsrBI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstUI       BstXI       BstZ17I     CelII       
CfoI        Cfr10I      ClaI        CpoI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoRV       EcoT14I     FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HpaI        KasI        
KspI        MluI        MslI        MvnI        NaeI        
NarI        NcoI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspBI       StuI        
StyI        SwaI        Tth111I     XbaI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

Acc65I       1453  GGTACC               G^GTACC
AccI         1340  GTCTAC               GT^[AC][GT]AC
AccIII        371  TCCGGA               T^CCGGA
AflIII        863  ACATGT               A^C[GA][CT]GT
Alw44I        181  GTGCAC               G^TGCAC
AocI          559  CCTGAGG              CC^T[ACGT]AGG
ApaI          975  GGGCCC               GGGCC^C
ApaLI         181  GTGCAC               G^TGCAC
Asp718I      1453  GGTACC               G^GTACC
BalI         1088  TGGCCA               TGG^CCA
BbrPI        1435  CACGTG               CAC^GTG
BbsI          455  GAAGAC               GAAGAC(2/6)
BciVI        1621  GGATAC               GTATCC(6/5)
BclI         1715  TGATCA               T^GATCA
BfaI           13  CTAG                 C^TAG
BglI           88  GCCTCCCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         455  GAAGAC               GAAGAC(2/6)
BsaAI        1435  CACGTG               [CT]AC^GT[GA]
BsaBI         469  GATGATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         371  TCCGGA               [AT]^CCGG[AT]
BseAI         371  TCCGGA               T^CCGGA
BsgI         1006  CTGCAC               GTGCAG(16/14)
BsmI          312  GAATGC               GAATGC(1/-1)
BspEI         371  TCCGGA               T^CCGGA
BspLU11I      863  ACATGT               A^CATGT
BsrDI        1386  CATTGC               GCAATG(2/0)
BstEII        134  GGTCACC              G^GT[ACGT]ACC
BstPI         134  GGTCACC              G^GT[ACGT]ACC
Bsu36I        559  CCTGAGG              CC^T[ACGT]AGG
DsaI          517  CCACGG               C^C[GA][CT]GG
EarI          798  CTCTTC               CTCTTC(1/4)
Eco81I        559  CCTGAGG              CC^T[ACGT]AGG
EcoO65I       134  GGTCACC              G^GT[ACGT]ACC
EcoRI         333  GAATTC               G^AATTC
EcoT22I      1093  ATGCAT               ATGCA^T
FauI         1104  CCCGC                CCCGC(4/6)
FbaI         1715  TGATCA               T^GATCA
KpnI         1453  GGTACC               GGTAC^C
Ksp632I       798  CTCTTC               CTCTTC(1/4)
MaeI           13  CTAG                 C^TAG
MaeII        1436  ACGT                 A^CGT
MamI          469  GATGATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        1088  TGGCCA               TGG^CCA
MroI          371  TCCGGA               T^CCGGA
MscI         1088  TGGCCA               TGG^CCA
NdeI         1642  CATATG               CA^TATG
NsiI         1093  ATGCAT               ATGCA^T
NspI          863  ACATGT               [GA]CATG^[CT]
PmaCI        1435  CACGTG               CAC^GTG
PmlI         1435  CACGTG               CAC^GTG
PpuMI         681  GGGTCCC              [GA]G^G[AT]CC[CT]
PstI         1108  CTGCAG               CTGCA^G
SmaI          106  CCCGGG               CCC^GGG
SmlI         1445  CTCAAG               C^T[CT][GA]AG
SspI         1249  AATATT               AAT^ATT
XcmI         1566  CCACCTCTACCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         106  CCCGGG               C^CCGGG
XmaI          106  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AcsI          333  GAATTC               [GA]^AATT[CT]
              718  AAATTC              
AlwNI         623  CAGGATCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1469  CAGCCCCTG           
ApoI          333  GAATTC               [GA]^AATT[CT]
              718  AAATTC              
AvaI          106  CCCGGG               C^[CT]CG[GA]G
              389  CTCGGG              
BcnI          106  CCCGG                CC^[GC]GG
              107  CCGGG               
BsoBI         106  CCCGGG               C^[CT]CG[GA]G
              389  CTCGGG              
BspMI         430  ACCTGC               ACCTGC(4/8)
              966  ACCTGC              
DraI          715  TTTAAA               TTT^AAA
             1260  TTTAAA              
EaeI         1088  TGGCCA               [CT]^GGCC[GA]
             1654  CGGCCA              
HapII         107  CCGG                 C^CGG
              372  CCGG                
HindIII        76  AAGCTT               A^AGCTT
             1269  AAGCTT              
HpaII         107  CCGG                 C^CGG
              372  CCGG                
MfeI          422  CAATTG               C^AATTG
             1293  CAATTG              
MseI          716  TTAA                 T^TAA
             1261  TTAA                
MspI          107  CCGG                 C^CGG
              372  CCGG                
MunI          422  CAATTG               C^AATTG
             1293  CAATTG              
NciI          106  CCCGG                CC^[GC]GG
              107  CCGGG               
PflMI          32  CCATAGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1666  CCACCTCCTGG         
PvuII          49  CAGCTG               CAG^CTG
              983  CAGCTG              
SfaNI        1095  GCATC                GCATC(5/9)
             1380  GCATC               
TfiI          567  GAATC                G^A[AT]TC
             1284  GAATC               
Tru9I         716  TTAA                 T^TAA
             1261  TTAA                
Van91I         32  CCATAGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1666  CCACCTCCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   61 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AspHI       
AvaII       BanI        BanII       BbvCI       BbvI        
BmyI        BpmI        BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmFI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
Cfr13I      DdeI        DpnII       DraII       EcoO109I    
EcoRII      Fnu4HI      FokI        HaeIII      HinfI       
HphI        ItaI        MaeIII      MboI        MboII       
MflI        MnlI        MspA1I      MvaI        MwoI        
NdeII       NlaIII      NlaIV       PleI        RsaI        
Sau3AI      Sau96I      ScrFI       SfcI        TaqI        
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII       
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