Restriction map for pF1KB4275
 (Linear) (Six-base) MAPSORT of: KIBB4275.seq  Check: 9536  from: 1  to: 1722



KIBB4275.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:37  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     439     975    1722 
   Size:        439     536     747

  Fragments arranged by size: 

                 747     536     439
 
AccI GT'mk_AC

Cuts at:      0    1341    1722 
   Size:       1341     381
 
Acc65I G'GTAC_C

Cuts at:      0    1453    1722 
   Size:       1453     269
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     568     644    1722 
   Size:        568      76    1078

  Fragments arranged by size: 

                1078     568      76
 
AflIII A'CryG_T

Cuts at:      0     863    1722 
   Size:        863     859
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     983    1403    1722 
   Size:        983     420     319

  Fragments arranged by size: 

                 983     420     319
 
AlwNI CAG_nnn'CTG

Cuts at:      0     628    1474    1722 
   Size:        628     846     248

  Fragments arranged by size: 

                 846     628     248
 
ApaI G_GGCC'C

Cuts at:      0     979    1722 
   Size:        979     743
 
ApaLI G'TGCA_C

Cuts at:      0     181    1722 
   Size:        181    1541
 
ApoI r'AATT_y

Cuts at:      0     333     718    1722 
   Size:        333     385    1004

  Fragments arranged by size: 

                1004     385     333
 
AvaI C'yCGr_G

Cuts at:      0     106     389    1722 
   Size:        106     283    1333

  Fragments arranged by size: 

                1333     283     106
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1576    1609    1722 
   Size:       1576      33     113

  Fragments arranged by size: 

                1576     113      33
 
BanI G'GyrC_C

Cuts at:      0     378     963    1453    1550    1675    1722 
   Size:        378     585     490      97     125      47

  Fragments arranged by size: 

                 585     490     378     125      97      47
 
BanII G_rGCy'C

Cuts at:      0     517     592     979    1722 
   Size:        517      75     387     743

  Fragments arranged by size: 

                 743     517     387      75
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     462    1722 
   Size:        462    1260
 
BbvCI CC'TCA_GC

Cuts at:      0     265     677     981    1722 
   Size:        265     412     304     741

  Fragments arranged by size: 

                 741     412     304     265
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1615    1722 
   Size:       1615     107
 
BclI T'GATC_A

Cuts at:      0    1715    1722 
   Size:       1715       7
 
BfrBI ATG'CAT

Cuts at:      0    1095    1722 
   Size:       1095     627
 
BglI GCCn_nnn'nGGC

Cuts at:      0      94    1722 
   Size:         94    1628
 
Bme1580I G_kGCm'C

Cuts at:      0     185     383     622     979    1442    1500    1553    1722 
   Size:        185     198     239     357     463      58      53     169

  Fragments arranged by size: 

                 463     357     239     198     185     169      58      53
            
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1716    1722 
   Size:       1716       6
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     813    1722 
   Size:        813     909
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     230     449     808    1722 
   Size:        230     219     359     914

  Fragments arranged by size: 

                 914     359     230     219
 
Bpu10I CC'TnA_GC

Cuts at:      0     160     265     677     981    1722 
   Size:        160     105     412     304     741

  Fragments arranged by size: 

                 741     412     304     160     105
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1430    1722 
   Size:       1430     292
 
BsaAI yAC'GTr

Cuts at:      0    1437    1722 
   Size:       1437     285
 
BsaBI GATnn'nnATC

Cuts at:      0     473    1722 
   Size:        473    1249
 
BsaWI w'CCGG_w

Cuts at:      0     371    1722 
   Size:        371    1351
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     231     795     989    1699    1722 
   Size:        231     564     194     710      23

  Fragments arranged by size: 

                 710     564     231     194      23
 
BseYI C'CCAG_C

Cuts at:      0      47      97     935     944    1054    1160    1632    1722 
   Size:         47      50     838       9     110     106     472      90

  Fragments arranged by size: 

                 838     472     110     106      90      50      47       9
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     991    1722 
   Size:        991     731
 
BsiHKAI G_wGCw'C

Cuts at:      0     185     999    1246    1722 
   Size:        185     814     247     476

  Fragments arranged by size: 

                 814     476     247     185
 
BsmI GAATG_Cn'

Cuts at:      0     318    1722 
   Size:        318    1404
 
Bsp1286I G_dGCh'C

Cuts at:      0     185     383     517     592     622     979     999    1246 
   Size:        185     198     134      75      30     357      20     247

Cuts at:   1246    1442    1500    1553    1722 
   Size:        196      58      53     169

  Fragments arranged by size: 

                 357     247     198     196     185     169     134      75
                  58      53      30      20
 
BspEI T'CCGG_A

Cuts at:      0     371    1722 
   Size:        371    1351
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     439     975    1722 
   Size:        439     536     747

  Fragments arranged by size: 

                 747     536     439
 
BsrDI GCAATG_nn'

Cuts at:      0    1385    1722 
   Size:       1385     337
 
BstEII G'GTnAC_C

Cuts at:      0     134    1722 
   Size:        134    1588
 
BstYI r'GATC_y

Cuts at:      0     144     284     625     747    1688    1722 
   Size:        144     140     341     122     941      34

  Fragments arranged by size: 

                 941     341     144     140     122      34
 
Bsu36I CC'TnA_GG

Cuts at:      0     560    1722 
   Size:        560    1162
 
BtgI C'CryG_G

Cuts at:      0     517    1722 
   Size:        517    1205
 
BtsI GCAGTG_nn'

Cuts at:      0     185     550     650    1003    1722 
   Size:        185     365     100     353     719

  Fragments arranged by size: 

                 719     365     353     185     100
 
DraI TTT'AAA

Cuts at:      0     717    1262    1722 
   Size:        717     545     460

  Fragments arranged by size: 

                 717     545     460
 
EaeI y'GGCC_r

Cuts at:      0    1088    1654    1722 
   Size:       1088     566      68

  Fragments arranged by size: 

                1088     566      68
 
EarI CTCTTCn'nnn_

Cuts at:      0     804    1722 
   Size:        804     918
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1075    1722 
   Size:       1075     647
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     230     449     568     644     808    1722 
   Size:        230     219     119      76     164     914

  Fragments arranged by size: 

                 914     230     219     164     119      76
 
EcoO109I rG'GnC_Cy

Cuts at:      0     682     975     976    1722 
   Size:        682     293       1     746

  Fragments arranged by size: 

                 746     682     293       1
 
EcoRI G'AATT_C

Cuts at:      0     333    1722 
   Size:        333    1389
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1287    1722 
   Size:       1287     435
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     463     495    1228    1260    1269    1301    1323    1355 
   Size:        463      32     733      32       9      32      22      32

Cuts at:   1355    1722 
   Size:        367

  Fragments arranged by size: 

                 733     463     367      32      32      32      32      22
                   9
 
HindIII A'AGCT_T

Cuts at:      0      76    1269    1722 
   Size:         76    1193     453

  Fragments arranged by size: 

                1193     453      76
 
KpnI G_GTAC'C

Cuts at:      0    1457    1722 
   Size:       1457     265
 
MfeI C'AATT_G

Cuts at:      0     422    1293    1722 
   Size:        422     871     429

  Fragments arranged by size: 

                 871     429     422
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     307    1722 
   Size:        307    1415
 
MscI TGG'CCA

Cuts at:      0    1090    1722 
   Size:       1090     632
 
MspA1I CmG'CkG

Cuts at:      0      51     281     985    1107    1722 
   Size:         51     230     704     122     615

  Fragments arranged by size: 

                 704     615     230     122      51
 
NdeI CA'TA_TG

Cuts at:      0    1643    1722 
   Size:       1643      79
 
NsiI A_TGCA'T

Cuts at:      0    1097    1722 
   Size:       1097     625
 
NspI r_CATG'y

Cuts at:      0     867    1722 
   Size:        867     855
 
PasI CC'CwG_GG

Cuts at:      0     400     972    1722 
   Size:        400     572     750

  Fragments arranged by size: 

                 750     572     400
 
PciI A'CATG_T

Cuts at:      0     863    1722 
   Size:        863     859
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      38    1672    1722 
   Size:         38    1634      50

  Fragments arranged by size: 

                1634      50      38
 
PmlI CAC'GTG

Cuts at:      0    1437    1722 
   Size:       1437     285
 
PpuMI rG'GwC_Cy

Cuts at:      0     682    1722 
   Size:        682    1040
 
PspOMI G'GGCC_C

Cuts at:      0     975    1722 
   Size:        975     747
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     844     876    1722 
   Size:        844      32     846

  Fragments arranged by size: 

                 846     844      32
 
PstI C_TGCA'G

Cuts at:      0    1112    1722 
   Size:       1112     610
 
PvuII CAG'CTG

Cuts at:      0      51     985    1722 
   Size:         51     934     737

  Fragments arranged by size: 

                 934     737      51
 
SanDI GG'GwC_CC

Cuts at:      0     682    1722 
   Size:        682    1040
 
SfcI C'TryA_G

Cuts at:      0     599     609     986    1108    1722 
   Size:        599      10     377     122     614

  Fragments arranged by size: 

                 614     599     377     122      10
 
SmaI CCC'GGG

Cuts at:      0     108    1722 
   Size:        108    1614
 
SmlI C'TyrA_G

Cuts at:      0    1445    1722 
   Size:       1445     277
 
SspI AAT'ATT

Cuts at:      0    1251    1722 
   Size:       1251     471
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     153    1568    1621    1722 
   Size:        153    1415      53     101

  Fragments arranged by size: 

                1415     153     101      53
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1573    1722 
   Size:       1573     149
 
XmaI C'CCGG_G

Cuts at:      0     106    1722 
   Size:        106    1616
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI   AflIII     AlfI    AlwNI     ApaI
    ApaLI     ApoI     AvaI     BaeI     BanI    BanII     BbsI    BbvCI
    BciVI     BclI    BfrBI     BglI Bme1580I     BmrI     BplI     BpmI
   Bpu10I    BpuEI    BsaAI    BsaBI    BsaWI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspEI    BspMI    BsrDI   BstEII    BstYI
   Bsu36I     BtgI     BtsI     DraI     EaeI     EarI     EciI  Eco57MI
 EcoO109I    EcoRI     FalI    Hin4I  HindIII     KpnI     MfeI     MmeI
     MscI   MspA1I     NdeI     NsiI     NspI     PasI     PciI    PflMI
     PmlI    PpuMI   PspOMI     PsrI     PstI    PvuII    SanDI     SfcI
     SmaI     SmlI     SspI    TaqII     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AclI     AfeI    AflII     AgeI     AhdI     AleI     AloI
     AscI     AseI    AsiSI    AvrII    BamHI     BbeI     BcgI    BglII
     BlpI    BmgBI     BmtI     BsaI    BsaHI    BsaXI    BsiEI    BsiWI
    BsmBI    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI    BstXI  BstZ17I    BtgZI     ClaI    CspCI   DraIII     DrdI
     EagI  EcoICRI    EcoNI    EcoRV     FseI     FspI    FspAI    HaeII
   HincII     HpaI     KasI     MluI     MslI     NaeI     NarI     NcoI
   NgoMIV     NheI     NotI     NruI     PacI     PfoI     PmeI     PpiI
    PshAI     PsiI    PspXI     PvuI    RsrII     SacI    SacII     SalI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI     TatI
     TstI  Tth111I     XbaI     XhoI     XmnI     ZraI
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