Restriction map for pF1KB4171
 (Linear) (Six-base) MAPSORT of: KIBB4171.seq  Check: 1578  from: 1  to: 3453



KIBB4171.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:37  ..

AccI GT'mk_AC

Cuts at:      0    1554    3453 
   Size:       1554    1899
 
Acc65I G'GTAC_C

Cuts at:      0    2208    3453 
   Size:       2208    1245
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2070    2614    2926    3453 
   Size:       2070     544     312     527

  Fragments arranged by size: 

                2070     544     527     312
 
AfeI AGC'GCT

Cuts at:      0     901    3453 
   Size:        901    2552
 
AflIII A'CryG_T

Cuts at:      0     198     638    1829    3453 
   Size:        198     440    1191    1624

  Fragments arranged by size: 

                1624    1191     440     198
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1101    3453 
   Size:       1101    2352
 
AleI CACnn'nnGTG

Cuts at:      0    3436    3453 
   Size:       3436      17
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2735    3370    3409    3453 
   Size:       2735     635      39      44

  Fragments arranged by size: 

                2735     635      44      39
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     505     537    1942    1974    3453 
   Size:        505      32    1405      32    1479

  Fragments arranged by size: 

                1479    1405     505      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     502    2871    3019    3064    3453 
   Size:        502    2369     148      45     389

  Fragments arranged by size: 

                2369     502     389     148      45
 
ApaI G_GGCC'C

Cuts at:      0    3096    3115    3453 
   Size:       3096      19     338

  Fragments arranged by size: 

                3096     338      19
 
ApoI r'AATT_y

Cuts at:      0     295    3453 
   Size:        295    3158
 
AvaI C'yCGr_G

Cuts at:      0     543    1339    2147    2366    2414    3414    3453 
   Size:        543     796     808     219      48    1000      39

  Fragments arranged by size: 

                1000     808     796     543     219      48      39
 
AvrII C'CTAG_G

Cuts at:      0     700    2055    3453 
   Size:        700    1355    1398

  Fragments arranged by size: 

                1398    1355     700
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    2836    2869    3453 
   Size:       2836      33     584

  Fragments arranged by size: 

                2836     584      33
 
BamHI G'GATC_C

Cuts at:      0     644     722    3453 
   Size:        644      78    2731

  Fragments arranged by size: 

                2731     644      78
 
BanI G'GyrC_C

Cuts at:      0    1123    1623    1710    2208    3296    3385    3453 
   Size:       1123     500      87     498    1088      89      68

  Fragments arranged by size: 

                1123    1088     500     498      89      87      68
 
BanII G_rGCy'C

Cuts at:      0     449    1037    1335    2405    2928    3096    3115    3180 
   Size:        449     588     298    1070     523     168      19      65

Cuts at:   3180    3421    3453 
   Size:        241      32

  Fragments arranged by size: 

                1070     588     523     449     298     241     168      65
                  32      19
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     770    1016    2005    2030    2632    3453 
   Size:        770     246     989      25     602     821

  Fragments arranged by size: 

                 989     821     770     602     246      25
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1990    2024    3453 
   Size:       1990      34    1429

  Fragments arranged by size: 

                1990    1429      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     545    3453 
   Size:        545    2908
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2128    2686    3331    3453 
   Size:       2128     558     645     122

  Fragments arranged by size: 

                2128     645     558     122
 
BglII A'GATC_T

Cuts at:      0    1379    3453 
   Size:       1379    2074
 
BlpI GC'TnA_GC

Cuts at:      0    2708    3453 
   Size:       2708     745
 
Bme1580I G_kGCm'C

Cuts at:      0     436    3096    3115    3299    3453 
   Size:        436    2660      19     184     154

  Fragments arranged by size: 

                2660     436     184     154      19
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      60     621    1105    3353    3453 
   Size:         60     561     484    2248     100

  Fragments arranged by size: 

                2248     561     484     100      60
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1761    2205    3453 
   Size:       1761     444    1248

  Fragments arranged by size: 

                1761    1248     444
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1358    1939    2056    2836    2869    3041    3083    3194 
   Size:       1358     581     117     780      33     172      42     111

Cuts at:   3194    3383    3453 
   Size:        189      70

  Fragments arranged by size: 

                1358     780     581     189     172     117     111      70
                  42      33
 
Bpu10I CC'TnA_GC

Cuts at:      0    1724    2333    3453 
   Size:       1724     609    1120

  Fragments arranged by size: 

                1724    1120     609
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2528    2761    3453 
   Size:       2528     233     692

  Fragments arranged by size: 

                2528     692     233
 
BsaAI yAC'GTr

Cuts at:      0     641    3453 
   Size:        641    2812
 
BsaBI GATnn'nnATC

Cuts at:      0     857    3453 
   Size:        857    2596
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     505     535     781     811    3453 
   Size:        505      30     246      30    2642

  Fragments arranged by size: 

                2642     505     246      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1015    2197    2359    2452    2497    2824    3248    3453 
   Size:       1015    1182     162      93      45     327     424     205

  Fragments arranged by size: 

                1182    1015     424     327     205     162      93      45
            
 
BseYI C'CCAG_C

Cuts at:      0     648    1279    1678    2996    3022    3182    3453 
   Size:        648     631     399    1318      26     160     271

  Fragments arranged by size: 

                1318     648     631     399     271     160      26
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     415     592    3453 
   Size:        415     177    2861

  Fragments arranged by size: 

                2861     415     177
 
BsiHKAI G_wGCw'C

Cuts at:      0    2116    3453 
   Size:       2116    1337
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1159    1711    3453 
   Size:       1159     552    1742

  Fragments arranged by size: 

                1742    1159     552
 
Bsp1286I G_dGCh'C

Cuts at:      0     436     449    1037    1335    2116    2405    2928    3096 
   Size:        436      13     588     298     781     289     523     168

Cuts at:   3096    3115    3180    3299    3421    3453 
   Size:         19      65     119     122      32

  Fragments arranged by size: 

                 781     588     523     436     298     289     168     122
                 119      65      32      19      13
 
BspHI T'CATG_A

Cuts at:      0     581    1490    3453 
   Size:        581     909    1963

  Fragments arranged by size: 

                1963     909     581
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     588    1241    3453 
   Size:        588     653    2212

  Fragments arranged by size: 

                2212     653     588
 
BsrBI CCG'CTC

Cuts at:      0     216    1326    2337    2351    2640    3453 
   Size:        216    1110    1011      14     289     813

  Fragments arranged by size: 

                1110    1011     813     289     216      14
 
BsrDI GCAATG_nn'

Cuts at:      0     213     363    2306    3453 
   Size:        213     150    1943    1147

  Fragments arranged by size: 

                1943    1147     213     150
 
BsrGI T'GTAC_A

Cuts at:      0     159    1832    1934    3453 
   Size:        159    1673     102    1519

  Fragments arranged by size: 

                1673    1519     159     102
 
BssSI C'ACGA_G

Cuts at:      0    1883    3453 
   Size:       1883    1570
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1876    3453 
   Size:       1876    1577
 
BstBI TT'CG_AA

Cuts at:      0    2238    3453 
   Size:       2238    1215
 
BstEII G'GTnAC_C

Cuts at:      0    3429    3453 
   Size:       3429      24
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2220    3453 
   Size:       2220    1233
 
BstYI r'GATC_y

Cuts at:      0     644     722    1379    3453 
   Size:        644      78     657    2074

  Fragments arranged by size: 

                2074     657     644      78
 
Bsu36I CC'TnA_GG

Cuts at:      0    1646    3453 
   Size:       1646    1807
 
BtgI C'CryG_G

Cuts at:      0      50    2213    3039    3225    3453 
   Size:         50    2163     826     186     228

  Fragments arranged by size: 

                2163     826     228     186      50
 
BtsI GCAGTG_nn'

Cuts at:      0    2307    2705    3093    3287    3370    3453 
   Size:       2307     398     388     194      83      83

  Fragments arranged by size: 

                2307     398     388     194      83      83
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2542    3453 
   Size:       2542     911
 
EaeI y'GGCC_r

Cuts at:      0     987    2687    2873    3453 
   Size:        987    1700     186     580

  Fragments arranged by size: 

                1700     987     580     186
 
EarI CTCTTCn'nnn_

Cuts at:      0     554     580     886     992    1052    2198    2518    3453 
   Size:        554      26     306     106      60    1146     320     935

  Fragments arranged by size: 

                1146     935     554     320     306     106      60      26
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1565    2290    3453 
   Size:       1565     725    1163

  Fragments arranged by size: 

                1565    1163     725
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1358    1939    2056    2070    2614    2836    2869    2926 
   Size:       1358     581     117      14     544     222      33      57

Cuts at:   2926    3041    3083    3194    3383    3453 
   Size:        115      42     111     189      70

  Fragments arranged by size: 

                1358     581     544     222     189     117     115     111
                  70      57      42      33      14
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1018    1237    1778    3453 
   Size:       1018     219     541    1675

  Fragments arranged by size: 

                1675    1018     541     219
 
EcoO109I rG'GnC_Cy

Cuts at:      0     955    1570    2059    3092    3093    3112    3453 
   Size:        955     615     489    1033       1      19     341

  Fragments arranged by size: 

                1033     955     615     489     341      19       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1456    3453 
   Size:       1456    1997
 
FspI TGC'GCA

Cuts at:      0    1822    3453 
   Size:       1822    1631
 
HaeII r_GCGC'y

Cuts at:      0     903    3453 
   Size:        903    2550
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1021    1053    1087    1119    1209    1241    1597    1629 
   Size:       1021      32      34      32      90      32     356      32

Cuts at:   1629    2500    2532    2910    2942    3453 
   Size:        871      32     378      32     511

  Fragments arranged by size: 

                1021     871     511     378     356      90      34      32
                  32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1083    1555    3143    3453 
   Size:       1083     472    1588     310

  Fragments arranged by size: 

                1588    1083     472     310
 
KpnI G_GTAC'C

Cuts at:      0    2212    3453 
   Size:       2212    1241
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     709    2765    2967    3453 
   Size:        709    2056     202     486

  Fragments arranged by size: 

                2056     709     486     202
 
MscI TGG'CCA

Cuts at:      0     989    3453 
   Size:        989    2464
 
MslI CAynn'nnrTG

Cuts at:      0    1592    1828    3436    3453 
   Size:       1592     236    1608      17

  Fragments arranged by size: 

                1608    1592     236      17
 
MspA1I CmG'CkG

Cuts at:      0      52     286    2671    2904    3172    3293    3337    3453 
   Size:         52     234    2385     233     268     121      44     116

  Fragments arranged by size: 

                2385     268     234     233     121     116      52      44
            
 
NcoI C'CATG_G

Cuts at:      0    3039    3225    3453 
   Size:       3039     186     228

  Fragments arranged by size: 

                3039     228     186
 
NspI r_CATG'y

Cuts at:      0     202    1806    1833    1969    3453 
   Size:        202    1604      27     136    1484

  Fragments arranged by size: 

                1604    1484     202     136      27
 
PciI A'CATG_T

Cuts at:      0     198    1829    3453 
   Size:        198    1631    1624

  Fragments arranged by size: 

                1631    1624     198
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1599    3045    3453 
   Size:       1599    1446     408

  Fragments arranged by size: 

                1599    1446     408
 
PfoI T'CCnGG_A

Cuts at:      0     725    1916    3099    3453 
   Size:        725    1191    1183     354

  Fragments arranged by size: 

                1191    1183     725     354
 
PmlI CAC'GTG

Cuts at:      0     641    3453 
   Size:        641    2812
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     505     537    3453 
   Size:        505      32    2916

  Fragments arranged by size: 

                2916     505      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     955    3453 
   Size:        955    2498
 
PspOMI G'GGCC_C

Cuts at:      0    3092    3111    3453 
   Size:       3092      19     342

  Fragments arranged by size: 

                3092     342      19
 
PstI C_TGCA'G

Cuts at:      0    1634    1872    3150    3453 
   Size:       1634     238    1278     303

  Fragments arranged by size: 

                1634    1278     303     238
 
PvuII CAG'CTG

Cuts at:      0     286    2904    3172    3337    3453 
   Size:        286    2618     268     165     116

  Fragments arranged by size: 

                2618     286     268     165     116
 
SacII CC_GC'GG

Cuts at:      0      53    3453 
   Size:         53    3400
 
SalI G'TCGA_C

Cuts at:      0    1553    3453 
   Size:       1553    1900
 
ScaI AGT'ACT

Cuts at:      0      40    3453 
   Size:         40    3413
 
SexAI A'CCwGG_T

Cuts at:      0    1535    1706    3453 
   Size:       1535     171    1747

  Fragments arranged by size: 

                1747    1535     171
 
SfcI C'TryA_G

Cuts at:      0    1630    1868    2432    3065    3146    3453 
   Size:       1630     238     564     633      81     307

  Fragments arranged by size: 

                1630     633     564     307     238      81
 
SmaI CCC'GGG

Cuts at:      0     545    3453 
   Size:        545    2908
 
StuI AGG'CCT

Cuts at:      0    2050    3453 
   Size:       2050    1403
 
StyI C'CwwG_G

Cuts at:      0     389     700    1673    2055    2219    2405    3039    3225 
   Size:        389     311     973     382     164     186     634     186

Cuts at:   3225    3453 
   Size:        228

  Fragments arranged by size: 

                 973     634     389     382     311     228     186     186
                 164
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     144    1147    2278    3314    3453 
   Size:        144    1003    1131    1036     139

  Fragments arranged by size: 

                1131    1036    1003     144     139
 
TatI w'GTAC_w

Cuts at:      0      38     159    1832    1934    3453 
   Size:         38     121    1673     102    1519

  Fragments arranged by size: 

                1673    1519     121     102      38
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    3020    3052    3453 
   Size:       3020      32     401

  Fragments arranged by size: 

                3020     401      32
 
XbaI T'CTAG_A

Cuts at:      0     552    3453 
   Size:        552    2901
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1134    2220    3453 
   Size:       1134    1086    1233

  Fragments arranged by size: 

                1233    1134    1086
 
XmaI C'CCGG_G

Cuts at:      0     543    3453 
   Size:        543    2910
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     AfeI   AflIII     AhdI     AleI     AlfI
     AloI    AlwNI     ApaI     ApoI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbsI     BcgI    BciVI     BglI    BglII     BlpI
 Bme1580I     BmrI     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I    BspHI
    BspMI    BsrBI    BsrDI    BsrGI    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI   Bsu36I     BtgI     BtsI     DrdI     EaeI     EarI
     EciI  Eco57MI    EcoNI EcoO109I     FalI     FspI    HaeII    Hin4I
   HincII     KpnI     MmeI     MscI     MslI   MspA1I     NcoI     NspI
     PciI    PflMI     PfoI     PmlI     PpiI    PpuMI   PspOMI     PstI
    PvuII    SacII     SalI     ScaI    SexAI     SfcI     SmaI     StuI
     StyI    TaqII     TatI     TstI     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AclI    AflII     AgeI    ApaLI     AscI     AseI
    AsiSI     BbeI    BbvCI     BclI    BfrBI    BmgBI     BmtI    BpuEI
    BsaHI    BsaWI    BsiEI    BsiWI     BsmI    BspEI    BsrFI   BssHII
  BstZ17I    BtgZI     ClaI    CspCI     DraI   DraIII     EagI  EcoICRI
    EcoRI    EcoRV     FseI    FspAI  HindIII     HpaI     KasI     MfeI
     MluI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PmeI    PshAI     PsiI    PspXI     PsrI
     PvuI    RsrII     SacI    SanDI     SapI     SbfI     SfiI     SfoI
     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI  Tth111I     XhoI     XmnI     ZraI
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