Restriction map for pF1KB4159
 (Linear) (Six-base) MAPSORT of: KIBB4159.seq  Check: 7051  from: 1  to: 435



KIBB4159.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:37  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     133     435 
   Size:        133     302
 
AgeI A'CCGG_T

Cuts at:      0      65     435 
   Size:         65     370
 
ApoI r'AATT_y

Cuts at:      0     349     435 
   Size:        349      86
 
BanII G_rGCy'C

Cuts at:      0     154     435 
   Size:        154     281
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     110     435 
   Size:        110     325
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     168     435 
   Size:        168     267
 
BsaWI w'CCGG_w

Cuts at:      0      65     435 
   Size:         65     370
 
Bsp1286I G_dGCh'C

Cuts at:      0     154     435 
   Size:        154     281
 
BsrFI r'CCGG_y

Cuts at:      0      65     435 
   Size:         65     370
 
BsrGI T'GTAC_A

Cuts at:      0     161     435 
   Size:        161     274
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     133     435 
   Size:        133     302
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     193     435 
   Size:        193     242
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     321     435 
   Size:        321     114
 
NspI r_CATG'y

Cuts at:      0     314     435 
   Size:        314     121
 
SfcI C'TryA_G

Cuts at:      0     237     435 
   Size:        237     198
 
StyI C'CwwG_G

Cuts at:      0     278     435 
   Size:        278     157
 
TatI w'GTAC_w

Cuts at:      0     161     435 
   Size:        161     274
 

 Enzymes that do cut:

     AcuI     AgeI     ApoI    BanII     BbsI     BplI    BsaWI Bsp1286I
    BsrFI    BsrGI  Eco57MI     FalI     MmeI     NspI     SfcI     StyI
     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI
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