Restriction map for pF1KB4141
 (Linear) (Six-base) MAPSORT of: KIBB4141.seq  Check: 3977  from: 1  to: 2247



KIBB4141.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:37  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1906    2247 
   Size:       1906     341
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     482    1542    1942    2001    2247 
   Size:        482    1060     400      59     246

  Fragments arranged by size: 

                1060     482     400     246      59
 
AfeI AGC'GCT

Cuts at:      0     988    2247 
   Size:        988    1259
 
AleI CACnn'nnGTG

Cuts at:      0     557    2247 
   Size:        557    1690
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1244    1720    2247 
   Size:       1244     476     527

  Fragments arranged by size: 

                1244     527     476
 
AlwNI CAG_nnn'CTG

Cuts at:      0      64     772    1275    2247 
   Size:         64     708     503     972

  Fragments arranged by size: 

                 972     708     503      64
 
ApaI G_GGCC'C

Cuts at:      0     439    1079    1470    2247 
   Size:        439     640     391     777

  Fragments arranged by size: 

                 777     640     439     391
 
ApaLI G'TGCA_C

Cuts at:      0     374    2247 
   Size:        374    1873
 
ApoI r'AATT_y

Cuts at:      0    1251    2247 
   Size:       1251     996
 
AvaI C'yCGr_G

Cuts at:      0      85     127     592     748     862    1462    1824    2247 
   Size:         85      42     465     156     114     600     362     423

  Fragments arranged by size: 

                 600     465     423     362     156     114      85      42
            
 
BanI G'GyrC_C

Cuts at:      0     616    1867    2247 
   Size:        616    1251     380

  Fragments arranged by size: 

                1251     616     380
 
BanII G_rGCy'C

Cuts at:      0      86     166     439     515    1079    1470    1768    2247 
   Size:         86      80     273      76     564     391     298     479

  Fragments arranged by size: 

                 564     479     391     298     273      86      80      76
            
 
BbeI G_GCGC'C

Cuts at:      0     620    2247 
   Size:        620    1627
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1097    1613    2194    2247 
   Size:       1097     516     581      53

  Fragments arranged by size: 

                1097     581     516      53
 
BglI GCCn_nnn'nGGC

Cuts at:      0     739     936    2247 
   Size:        739     197    1311

  Fragments arranged by size: 

                1311     739     197
 
BlpI GC'TnA_GC

Cuts at:      0    1834    2066    2247 
   Size:       1834     232     181

  Fragments arranged by size: 

                1834     232     181
 
Bme1580I G_kGCm'C

Cuts at:      0     128     378     439    1079    1470    2247 
   Size:        128     250      61     640     391     777

  Fragments arranged by size: 

                 777     640     391     250     128      61
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1533    2247 
   Size:       1533     714
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     157     198     676     985    1732    2247 
   Size:        157      41     478     309     747     515

  Fragments arranged by size: 

                 747     515     478     309     157      41
 
Bpu10I CC'TnA_GC

Cuts at:      0     761    2247 
   Size:        761    1486
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      37     544    1632    1984    2247 
   Size:         37     507    1088     352     263

  Fragments arranged by size: 

                1088     507     352     263      37
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      48     875    1447    2224    2247 
   Size:         48     827     572     777      23

  Fragments arranged by size: 

                 827     777     572      48      23
 
BsaAI yAC'GTr

Cuts at:      0     228    2247 
   Size:        228    2019
 
BsaHI Gr'CG_yC

Cuts at:      0     617    1416    2078    2247 
   Size:        617     799     662     169

  Fragments arranged by size: 

                 799     662     617     169
 
BsaWI w'CCGG_w

Cuts at:      0       8     501    2247 
   Size:          8     493    1746

  Fragments arranged by size: 

                1746     493       8
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     259     262     289     292     295     370     430     610 
   Size:        259       3      27       3       3      75      60     180

Cuts at:    610     682    2206    2247 
   Size:         72    1524      41

  Fragments arranged by size: 

                1524     259     180      75      72      60      41      27
                   3       3       3
 
BseYI C'CCAG_C

Cuts at:      0     820    1139    1526    1760    2010    2247 
   Size:        820     319     387     234     250     237

  Fragments arranged by size: 

                 820     387     319     250     237     234
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2044    2247 
   Size:       2044     203
 
BsiEI CG_ry'CG

Cuts at:      0    2171    2247 
   Size:       2171      76
 
BsiHKAI G_wGCw'C

Cuts at:      0     378    2247 
   Size:        378    1869
 
Bsp1286I G_dGCh'C

Cuts at:      0      86     128     166     378     439     515    1079    1470 
   Size:         86      42      38     212      61      76     564     391

Cuts at:   1470    1768    2247 
   Size:        298     479

  Fragments arranged by size: 

                 564     479     391     298     212      86      76      61
                  42      38
 
BspEI T'CCGG_A

Cuts at:      0     501    2247 
   Size:        501    1746
 
BspHI T'CATG_A

Cuts at:      0    2048    2247 
   Size:       2048     199
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     939    1906    2247 
   Size:        939     967     341

  Fragments arranged by size: 

                 967     939     341
 
BsrBI CCG'CTC

Cuts at:      0      32    1823    2247 
   Size:         32    1791     424

  Fragments arranged by size: 

                1791     424      32
 
BsrDI GCAATG_nn'

Cuts at:      0     963    2060    2247 
   Size:        963    1097     187

  Fragments arranged by size: 

                1097     963     187
 
BsrFI r'CCGG_y

Cuts at:      0     613    2247 
   Size:        613    1634
 
BsrGI T'GTAC_A

Cuts at:      0    1655    2240    2247 
   Size:       1655     585       7

  Fragments arranged by size: 

                1655     585       7
 
BssSI C'ACGA_G

Cuts at:      0     454     829     943    2137    2247 
   Size:        454     375     114    1194     110

  Fragments arranged by size: 

                1194     454     375     114     110
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     344    2247 
   Size:        344    1903
 
BstEII G'GTnAC_C

Cuts at:      0    1968    2247 
   Size:       1968     279
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1128    1878    2247 
   Size:       1128     750     369

  Fragments arranged by size: 

                1128     750     369
 
BstYI r'GATC_y

Cuts at:      0    1730    2177    2247 
   Size:       1730     447      70

  Fragments arranged by size: 

                1730     447      70
 
Bsu36I CC'TnA_GG

Cuts at:      0     272     314     634     773    2247 
   Size:        272      42     320     139    1474

  Fragments arranged by size: 

                1474     320     272     139      42
 
BtgI C'CryG_G

Cuts at:      0     447    1778    1973    2247 
   Size:        447    1331     195     274

  Fragments arranged by size: 

                1331     447     274     195
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1362    1518    2247 
   Size:       1362     156     729

  Fragments arranged by size: 

                1362     729     156
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1022    1043    1057    1078    2247 
   Size:       1022      21      14      21    1169

  Fragments arranged by size: 

                1169    1022      21      21      14
 
DraI TTT'AAA

Cuts at:      0    1322    2247 
   Size:       1322     925
 
DraIII CAC_nnn'GTG

Cuts at:      0     384    2247 
   Size:        384    1863
 
EaeI y'GGCC_r

Cuts at:      0     154     450     486    1409    1548    1790    1990    2126 
   Size:        154     296      36     923     139     242     200     136

Cuts at:   2126    2247 
   Size:        121

  Fragments arranged by size: 

                 923     296     242     200     154     139     136     121
                  36
 
EarI CTCTTCn'nnn_

Cuts at:      0     917    1297    2247 
   Size:        917     380     950

  Fragments arranged by size: 

                 950     917     380
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     157     198     482     676     985    1542    1732    1942 
   Size:        157      41     284     194     309     557     190     210

Cuts at:   1942    2001    2247 
   Size:         59     246

  Fragments arranged by size: 

                 557     309     284     246     210     194     190     157
                  59      41
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     271     764     862    2247 
   Size:        271     493      98    1385

  Fragments arranged by size: 

                1385     493     271      98
 
EcoO109I rG'GnC_Cy

Cuts at:      0      61     132     264     651     769     914    1075    1076 
   Size:         61      71     132     387     118     145     161       1

Cuts at:   1076    1466    1917    2247 
   Size:        390     451     330

  Fragments arranged by size: 

                 451     390     387     330     161     145     132     118
                  71      61       1
 
EcoRI G'AATT_C

Cuts at:      0    1251    2247 
   Size:       1251     996
 
HaeII r_GCGC'y

Cuts at:      0     620     734     990    2247 
   Size:        620     114     256    1257

  Fragments arranged by size: 

                1257     620     256     114
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      45      77     196     202     228     234     499     531 
   Size:         45      32     119       6      26       6     265      32

Cuts at:    531    2063    2095    2247 
   Size:       1532      32     152

  Fragments arranged by size: 

                1532     265     152     119      45      32      32      32
                  26       6       6
 
HincII GTy'rAC

Cuts at:      0    1011    2247 
   Size:       1011    1236
 
HindIII A'AGCT_T

Cuts at:      0      13    2247 
   Size:         13    2234
 
KasI G'GCGC_C

Cuts at:      0     616    2247 
   Size:        616    1631
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      20    1496    2151    2186    2247 
   Size:         20    1476     655      35      61

  Fragments arranged by size: 

                1476     655      61      35      20
 
MscI TGG'CCA

Cuts at:      0    1411    1792    2128    2247 
   Size:       1411     381     336     119

  Fragments arranged by size: 

                1411     381     336     119
 
MslI CAynn'nnrTG

Cuts at:      0     557    1177    1399    2247 
   Size:        557     620     222     848

  Fragments arranged by size: 

                 848     620     557     222
 
MspA1I CmG'CkG

Cuts at:      0     812    1143    1710    1958    2010    2238    2247 
   Size:        812     331     567     248      52     228       9

  Fragments arranged by size: 

                 812     567     331     248     228      52       9
 
NarI GG'CG_CC

Cuts at:      0     617    2247 
   Size:        617    1630
 
NspI r_CATG'y

Cuts at:      0      39    1425    2247 
   Size:         39    1386     822

  Fragments arranged by size: 

                1386     822      39
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     687    2247 
   Size:        687    1560
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     223     255    2247 
   Size:        223      32    1992

  Fragments arranged by size: 

                1992     223      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      61     651     914    2247 
   Size:         61     590     263    1333

  Fragments arranged by size: 

                1333     590     263      61
 
PspOMI G'GGCC_C

Cuts at:      0     435    1075    1466    2247 
   Size:        435     640     391     781

  Fragments arranged by size: 

                 781     640     435     391
 
PstI C_TGCA'G

Cuts at:      0     817    1709    2044    2247 
   Size:        817     892     335     203

  Fragments arranged by size: 

                 892     817     335     203
 
PvuII CAG'CTG

Cuts at:      0     812    1143    1710    1958    2010    2247 
   Size:        812     331     567     248      52     237

  Fragments arranged by size: 

                 812     567     331     248     237      52
 
SexAI A'CCwGG_T

Cuts at:      0      57    2247 
   Size:         57    2190
 
SfcI C'TryA_G

Cuts at:      0     782     813    1705    2040    2247 
   Size:        782      31     892     335     207

  Fragments arranged by size: 

                 892     782     335     207      31
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     936    2247 
   Size:        936    1311
 
SfoI GGC'GCC

Cuts at:      0     618    2247 
   Size:        618    1629
 
SgrAI Cr'CCGG_yG

Cuts at:      0     613    2247 
   Size:        613    1634
 
SmaI CCC'GGG

Cuts at:      0    1464    2247 
   Size:       1464     783
 
SmlI C'TyrA_G

Cuts at:      0      16     523    1611    1999    2247 
   Size:         16     507    1088     388     248

  Fragments arranged by size: 

                1088     507     388     248      16
 
SphI G_CATG'C

Cuts at:      0    1425    2247 
   Size:       1425     822
 
StuI AGG'CCT

Cuts at:      0     790    1555    2247 
   Size:        790     765     692

  Fragments arranged by size: 

                 790     765     692
 
StyI C'CwwG_G

Cuts at:      0     110     973    1902    2247 
   Size:        110     863     929     345

  Fragments arranged by size: 

                 929     863     345     110
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     813    1153    1318    1660    2157    2247 
   Size:        813     340     165     342     497      90

  Fragments arranged by size: 

                 813     497     342     340     165      90
 
TatI w'GTAC_w

Cuts at:      0    1655    2240    2247 
   Size:       1655     585       7

  Fragments arranged by size: 

                1655     585       7
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     169     201    2247 
   Size:        169      32    2046

  Fragments arranged by size: 

                2046     169      32
 
Tth111I GACn'n_nGTC

Cuts at:      0      59    2247 
   Size:         59    2188
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     202     544    1785    2247 
   Size:        202     342    1241     462

  Fragments arranged by size: 

                1241     462     342     202
 
XmaI C'CCGG_G

Cuts at:      0    1462    2247 
   Size:       1462     785
 

 Enzymes that do cut:

     AarI     AcuI     AfeI     AleI     AlfI    AlwNI     ApaI    ApaLI
     ApoI     AvaI     BanI    BanII     BbeI     BbsI     BglI     BlpI
 Bme1580I     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI
    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI
   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI    CspCI     DraI
   DraIII     EaeI     EarI  Eco57MI    EcoNI EcoO109I    EcoRI    HaeII
    Hin4I   HincII  HindIII     KasI     MmeI     MscI     MslI   MspA1I
     NarI     NspI    PflMI     PpiI    PpuMI   PspOMI     PstI    PvuII
    SexAI     SfcI     SfiI     SfoI    SgrAI     SmaI     SmlI     SphI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AloI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BbvCI
     BcgI    BciVI     BclI    BfrBI    BglII    BmgBI     BmtI     BplI
    BsaBI    BsaXI    BsiWI     BsmI    BsmBI   BssHII    BstBI  BstZ17I
     BtsI     ClaI     DrdI     EagI     EciI  EcoICRI    EcoRV     FalI
     FseI     FspI    FspAI     HpaI     KpnI     MfeI     MluI     NaeI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI     PfoI     PmeI     PmlI    PshAI     PsiI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI     SgfI    SnaBI     SpeI     SrfI     SspI     SwaI
     XbaI     XhoI     XmnI     ZraI
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