Restriction map for pF1KB4140
##################################
# NO CUT ENZYMES (   97 enzymes) #
##################################

Acc65I      AclI        AflII       AgeI        AhdI        
AlwI        Aor51HI     AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvrII       
BamHI       BbeI        BciVI       BclI        BfrI        
BlnI        BlpI        Bpu1102I    BsaBI       BsiWI       
BsmBI       BsmI        BspDI       BsrBI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstZ17I     CelII       ClaI        CpoI        DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoNI       EcoO65I     EcoRV       EcoT22I     FbaI        
HindIII     HpaI        KasI        KpnI        MamI        
MfeI        MluI        MslI        MunI        NarI        
NcoI        NdeI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
RsrII       SacI        SapI        SbfI        ScaI        
SexAI       SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
SwaI        Tth111I     XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AatII         256  GACGTC               GACGT^C
AccIII        722  TCCGGA               T^CCGGA
Alw44I       1327  GTGCAC               G^TGCAC
ApaLI        1327  GTGCAC               G^TGCAC
AviII         644  TGCGCA               TGC^GCA
BbrPI         477  CACGTG               CAC^GTG
BbsI          253  GAAGAC               GAAGAC(2/6)
BglII         893  AGATCT               A^GATCT
BpuAI         253  GAAGAC               GAAGAC(2/6)
BsaI          310  GAGACC               GGTCTC(1/5)
BsaWI         722  TCCGGA               [AT]^CCGG[AT]
BseAI         722  TCCGGA               T^CCGGA
BsiEI          94  CGGTCG               CG[GA][CT]^CG
Bsp1407I     2180  TGTACA               T^GTACA
BspEI         722  TCCGGA               T^CCGGA
BspHI         471  TCATGA               T^CATGA
BspLU11I     1782  ACATGT               A^CATGT
BsrDI        1949  CATTGC               GCAATG(2/0)
BsrGI        2180  TGTACA               T^GTACA
BstAPI        429  GCAGACGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI        2024  CCAAGCAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         893  AGATCT               [GA]^GATC[CT]
DraI         1111  TTTAAA               TTT^AAA
EcoRI        1722  GAATTC               G^AATTC
FspI          644  TGCGCA               TGC^GCA
KspI           91  CCGCGG               CCGC^GG
MaeIII        691  GTCAC                ^GT[ACGT]AC
MflI          893  AGATCT               [GA]^GATC[CT]
MroI          722  TCCGGA               T^CCGGA
MseI         1112  TTAA                 T^TAA
NheI         1357  GCTAGC               G^CTAGC
NspI         1782  ACATGT               [GA]CATG^[CT]
PmaCI         477  CACGTG               CAC^GTG
PmlI          477  CACGTG               CAC^GTG
RcaI          471  TCATGA               T^CATGA
SacII          91  CCGCGG               CCGC^GG
SfaNI         218  GCATC                GCATC(5/9)
SfiI         2218  GGCCATGCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI        2180  TGTACA               T^GTACA
StuI          374  AGGCCT               AGG^CCT
Tru9I        1112  TTAA                 T^TAA
Tsp45I        691  GTCAC                ^GT[GC]AC
XhoII         893  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AcsI         1094  AAATTC               [GA]^AATT[CT]
             1722  GAATTC              
AcyI          256  GACGTC               G[GA]^CG[CT]C
              454  GACGCC              
AfaI          558  GTAC                 GT^AC
             2181  GTAC                
AflIII        476  ACACGT               A^C[GA][CT]GT
             1782  ACATGT              
AocI         1801  CCTGAGG              CC^T[ACGT]AGG
             2044  CCTGAGG             
ApaI         1989  GGGCCC               GGGCC^C
             2207  GGGCCC              
ApoI         1094  AAATTC               [GA]^AATT[CT]
             1722  GAATTC              
AvaI          740  CCCGAG               C^[CT]CG[GA]G
             2063  CCCGAG              
BanI         1141  GGTGCC               G^G[CT][GA]CC
             1468  GGTGCC              
BbvCI         304  GCTGAGG              CCTCAGC(-5/-2)
             1792  GCTGAGG             
BcgI          218  GCATCGCCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1248  GCAGGCAGCTCG        
BsaAI         477  CACGTG               [CT]AC^GT[GA]
             1324  TACGTG              
BsaHI         256  GACGTC               G[GA]^CG[CT]C
              454  GACGCC              
BsoBI         740  CCCGAG               C^[CT]CG[GA]G
             2063  CCCGAG              
Bsu36I       1801  CCTGAGG              CC^T[ACGT]AGG
             2044  CCTGAGG             
DsaI           91  CCGCGG               C^C[GA][CT]GG
              185  CCGTGG              
EarI          553  GAAGAG               CTCTTC(1/4)
             1083  GAAGAG              
Eco81I       1801  CCTGAGG              CC^T[ACGT]AGG
             2044  CCTGAGG             
FseI          272  GGCCGGCC             GGCCGG^CC
             1027  GGCCGGCC            
Hin1I         256  GACGTC               G[GA]^CG[CT]C
              454  GACGCC              
HincII       1811  GTCGAC               GT[CT]^[GA]AC
             2035  GTCGAC              
HindII       1811  GTCGAC               GT[CT]^[GA]AC
             2035  GTCGAC              
Ksp632I       553  GAAGAG               CTCTTC(1/4)
             1083  GAAGAG              
PflMI         409  CCAGTGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              863  CCACTGTTTGG         
RsaI          558  GTAC                 GT^AC
             2181  GTAC                
SalI         1811  GTCGAC               G^TCGAC
             2035  GTCGAC              
Van91I        409  CCAGTGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              863  CCACTGTTTGG         
XcmI          890  CCAAGATCTACATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1388  CCATGCACCGGGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   90 enzymes) #
#########################################

AccI        AccII       AciI        AluI        AlwNI       
AspHI       AvaII       BalI        BanII       BbvI        
BcnI        BfaI        BglI        BmyI        BpmI        
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BspMI       
BsrFI       BsrI        BssKI       BstF5I      BstNI       
BstUI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DraIII      EaeI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        MaeI        MaeII       MboI        MboII       
MluNI       MnlI        MscI        MspA1I      MspI        
MvaI        MvnI        MwoI        NaeI        NciI        
NdeII       NgoMIV      NlaIII      NlaIV       PleI        
PpuMI       PstI        PvuII       Sau3AI      Sau96I      
ScrFI       SfcI        SmlI        StyI        TaqI        
TfiI        TseI        Tsp509I     TspRI       TthHB8I     
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