Restriction map for pF1KB4140
 (Linear) (Six-base) MAPSORT of: KIBB4140.seq  Check: 7545  from: 1  to: 2241



KIBB4140.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:37  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1456    2085    2241 
   Size:       1456     629     156

  Fragments arranged by size: 

                1456     629     156
 
AatII G_ACGT'C

Cuts at:      0     260    2241 
   Size:        260    1981
 
AccI GT'mk_AC

Cuts at:      0    1268    1812    2036    2241 
   Size:       1268     544     224     205

  Fragments arranged by size: 

                1268     544     224     205
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1867    2233    2241 
   Size:       1867     366       8

  Fragments arranged by size: 

                1867     366       8
 
AflIII A'CryG_T

Cuts at:      0     476    1782    2241 
   Size:        476    1306     459

  Fragments arranged by size: 

                1306     476     459
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1394    2241 
   Size:       1394     847
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1474    1506    2241 
   Size:       1474      32     735

  Fragments arranged by size: 

                1474     735      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     179     415     435     978    1945    2091    2100    2135 
   Size:        179     236      20     543     967     146       9      35

Cuts at:   2135    2241 
   Size:        106

  Fragments arranged by size: 

                 967     543     236     179     146     106      35      20
                   9
 
ApaI G_GGCC'C

Cuts at:      0    1993    2211    2241 
   Size:       1993     218      30

  Fragments arranged by size: 

                1993     218      30
 
ApaLI G'TGCA_C

Cuts at:      0    1327    2241 
   Size:       1327     914
 
ApoI r'AATT_y

Cuts at:      0    1094    1722    2241 
   Size:       1094     628     519

  Fragments arranged by size: 

                1094     628     519
 
AvaI C'yCGr_G

Cuts at:      0     740    2063    2241 
   Size:        740    1323     178

  Fragments arranged by size: 

                1323     740     178
 
BanI G'GyrC_C

Cuts at:      0    1141    1468    2241 
   Size:       1141     327     773

  Fragments arranged by size: 

                1141     773     327
 
BanII G_rGCy'C

Cuts at:      0     169     806    1704    1993    2147    2211    2241 
   Size:        169     637     898     289     154      64      30

  Fragments arranged by size: 

                 898     637     289     169     154      64      30
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     260    2241 
   Size:        260    1981
 
BbvCI CC'TCA_GC

Cuts at:      0     305    1793    2241 
   Size:        305    1488     448

  Fragments arranged by size: 

                1488     448     305
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     207     241    1237    1271    2241 
   Size:        207      34     996      34     970

  Fragments arranged by size: 

                 996     970     207      34      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1911    1988    2225    2241 
   Size:       1911      77     237      16

  Fragments arranged by size: 

                1911     237      77      16
 
BglII A'GATC_T

Cuts at:      0     893    2241 
   Size:        893    1348
 
Bme1580I G_kGCm'C

Cuts at:      0    1331    1473    1559    1993    2211    2241 
   Size:       1331     142      86     434     218      30

  Fragments arranged by size: 

                1331     434     218     142      86      30
 
BmgBI CAC'GTC

Cuts at:      0     236     426    1569    2241 
   Size:        236     190    1143     672

  Fragments arranged by size: 

                1143     672     236     190
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1482    2241 
   Size:       1482     759
 
BmtI G_CTAG'C

Cuts at:      0    1361    2241 
   Size:       1361     880
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     288     481     523     538    1726    2077    2241 
   Size:        288     193      42      15    1188     351     164

  Fragments arranged by size: 

                1188     351     288     193     164      42      15
 
Bpu10I CC'TnA_GC

Cuts at:      0     305     393    1793    1865    2241 
   Size:        305      88    1400      72     376

  Fragments arranged by size: 

                1400     376     305      88      72
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1057    1576    1944    2241 
   Size:       1057     519     368     297

  Fragments arranged by size: 

                1057     519     368     297
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     304    2241 
   Size:        304    1937
 
BsaAI yAC'GTr

Cuts at:      0     479    1326    2241 
   Size:        479     847     915

  Fragments arranged by size: 

                 915     847     479
 
BsaHI Gr'CG_yC

Cuts at:      0     257     455    2241 
   Size:        257     198    1786

  Fragments arranged by size: 

                1786     257     198
 
BsaWI w'CCGG_w

Cuts at:      0     722    2241 
   Size:        722    1519
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0       4      34    2241 
   Size:          4      30    2207

  Fragments arranged by size: 

                2207      30       4
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      18     322     535    2241 
   Size:         18     304     213    1706

  Fragments arranged by size: 

                1706     304     213      18
 
BseYI C'CCAG_C

Cuts at:      0     162     486     623    1308    1474    1639    2100    2241 
   Size:        162     324     137     685     166     165     461     141

  Fragments arranged by size: 

                 685     461     324     166     165     162     141     137
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     341     448     902     907    1036    1267    1522    1684 
   Size:        341     107     454       5     129     231     255     162

Cuts at:   1684    1810    1829    2241 
   Size:        126      19     412

  Fragments arranged by size: 

                 454     412     341     255     231     162     129     126
                 107      19       5
 
BsiEI CG_ry'CG

Cuts at:      0      97    2241 
   Size:         97    2144
 
BsiHKAI G_wGCw'C

Cuts at:      0     755    1331    1871    2241 
   Size:        755     576     540     370

  Fragments arranged by size: 

                 755     576     540     370
 
Bsp1286I G_dGCh'C

Cuts at:      0     169     755     806    1331    1473    1559    1704    1871 
   Size:        169     586      51     525     142      86     145     167

Cuts at:   1871    1993    2147    2211    2241 
   Size:        122     154      64      30

  Fragments arranged by size: 

                 586     525     169     167     154     145     142     122
                  86      64      51      30
 
BspEI T'CCGG_A

Cuts at:      0     722    2241 
   Size:        722    1519
 
BspHI T'CATG_A

Cuts at:      0     471    2241 
   Size:        471    1770
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     722     794     941    1456    2085    2241 
   Size:        722      72     147     515     629     156

  Fragments arranged by size: 

                 722     629     515     156     147      72
 
BsrDI GCAATG_nn'

Cuts at:      0    1948    2241 
   Size:       1948     293
 
BsrFI r'CCGG_y

Cuts at:      0     273    1028    1788    2241 
   Size:        273     755     760     453

  Fragments arranged by size: 

                 760     755     453     273
 
BsrGI T'GTAC_A

Cuts at:      0    2180    2241 
   Size:       2180      61
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     435    2241 
   Size:        435    1806
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2031    2241 
   Size:       2031     210
 
BstYI r'GATC_y

Cuts at:      0     893    2241 
   Size:        893    1348
 
Bsu36I CC'TnA_GG

Cuts at:      0    1802    2045    2241 
   Size:       1802     243     196

  Fragments arranged by size: 

                1802     243     196
 
BtgI C'CryG_G

Cuts at:      0      91     185    2241 
   Size:         91      94    2056

  Fragments arranged by size: 

                2056      94      91
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     204    1708    2241 
   Size:        204    1504     533

  Fragments arranged by size: 

                1504     533     204
 
BtsI GCAGTG_nn'

Cuts at:      0     213     842     919    1010    1510    1921    2241 
   Size:        213     629      77      91     500     411     320

  Fragments arranged by size: 

                 629     500     411     320     213      91      77
 
DraI TTT'AAA

Cuts at:      0    1113    2241 
   Size:       1113    1128
 
DraIII CAC_nnn'GTG

Cuts at:      0    1059    1398    1516    2241 
   Size:       1059     339     118     725

  Fragments arranged by size: 

                1059     725     339     118
 
EaeI y'GGCC_r

Cuts at:      0     210     248    1010    1026    1507    2174    2226    2241 
   Size:        210      38     762      16     481     667      52      15

  Fragments arranged by size: 

                 762     667     481     210      52      38      16      15
            
 
EarI CTCTTCn'nnn_

Cuts at:      0     548    1078    2241 
   Size:        548     530    1163

  Fragments arranged by size: 

                1163     548     530
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     154    2241 
   Size:        154    2087
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     288     481     523     538    1726    1867    2077    2233 
   Size:        288     193      42      15    1188     141     210     156

Cuts at:   2233    2241 
   Size:          8

  Fragments arranged by size: 

                1188     288     210     193     156     141      42      15
                   8
 
EcoO109I rG'GnC_Cy

Cuts at:      0     767    1302    1482    1798    1989    1990    2207    2208 
   Size:        767     535     180     316     191       1     217       1

Cuts at:   2208    2241 
   Size:         33

  Fragments arranged by size: 

                 767     535     316     217     191     180      33       1
                   1
 
EcoRI G'AATT_C

Cuts at:      0    1722    2241 
   Size:       1722     519
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1617    2241 
   Size:       1617     624
 
FseI GG_CCGG'CC

Cuts at:      0     277    1032    2241 
   Size:        277     755    1209

  Fragments arranged by size: 

                1209     755     277
 
FspI TGC'GCA

Cuts at:      0     646    2241 
   Size:        646    1595
 
HaeII r_GCGC'y

Cuts at:      0      80     103     179     247     978    2241 
   Size:         80      23      76      68     731    1263

  Fragments arranged by size: 

                1263     731      80      76      68      23
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     688     720     898     930    2241 
   Size:        688      32     178      32    1311

  Fragments arranged by size: 

                1311     688     178      32      32
 
HincII GTy'rAC

Cuts at:      0    1813    2037    2241 
   Size:       1813     224     204

  Fragments arranged by size: 

                1813     224     204
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     140    2241 
   Size:        140    2101
 
MscI TGG'CCA

Cuts at:      0    1012    1509    2176    2228    2241 
   Size:       1012     497     667      52      13

  Fragments arranged by size: 

                1012     667     497      52      13
 
MspA1I CmG'CkG

Cuts at:      0      17      93     123    1417    1639    1648    2031    2055 
   Size:         17      76      30    1294     222       9     383      24

Cuts at:   2055    2241 
   Size:        186

  Fragments arranged by size: 

                1294     383     222     186      76      30      24      17
                   9
 
NaeI GCC'GGC

Cuts at:      0     275    1030    1790    2241 
   Size:        275     755     760     451

  Fragments arranged by size: 

                 760     755     451     275
 
NgoMIV G'CCGG_C

Cuts at:      0     273    1028    1788    2241 
   Size:        273     755     760     453

  Fragments arranged by size: 

                 760     755     453     273
 
NheI G'CTAG_C

Cuts at:      0    1357    2241 
   Size:       1357     884
 
NspI r_CATG'y

Cuts at:      0    1786    2241 
   Size:       1786     455
 
PasI CC'CwG_GG

Cuts at:      0    1738    2241 
   Size:       1738     503
 
PciI A'CATG_T

Cuts at:      0    1782    2241 
   Size:       1782     459
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     415     869    2241 
   Size:        415     454    1372

  Fragments arranged by size: 

                1372     454     415
 
PfoI T'CCnGG_A

Cuts at:      0     962    1622    2241 
   Size:        962     660     619

  Fragments arranged by size: 

                 962     660     619
 
PmlI CAC'GTG

Cuts at:      0     479    2241 
   Size:        479    1762
 
PpuMI rG'GwC_Cy

Cuts at:      0     767    1302    1482    1798    2241 
   Size:        767     535     180     316     443

  Fragments arranged by size: 

                 767     535     443     316     180
 
PspOMI G'GGCC_C

Cuts at:      0    1989    2207    2241 
   Size:       1989     218      34

  Fragments arranged by size: 

                1989     218      34
 
PstI C_TGCA'G

Cuts at:      0     137     331     791    1638    2096    2238    2241 
   Size:        137     194     460     847     458     142       3

  Fragments arranged by size: 

                 847     460     458     194     142     137       3
 
PvuII CAG'CTG

Cuts at:      0    1639    1648    2031    2055    2241 
   Size:       1639       9     383      24     186

  Fragments arranged by size: 

                1639     383     186      24       9
 
SacII CC_GC'GG

Cuts at:      0      94    2241 
   Size:         94    2147
 
SalI G'TCGA_C

Cuts at:      0    1811    2035    2241 
   Size:       1811     224     206

  Fragments arranged by size: 

                1811     224     206
 
SfcI C'TryA_G

Cuts at:      0     133     327     787    1101    1634    2092    2234    2241 
   Size:        133     194     460     314     533     458     142       7

  Fragments arranged by size: 

                 533     460     458     314     194     142     133       7
            
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    2225    2241 
   Size:       2225      16
 
SmlI C'TyrA_G

Cuts at:      0    1072    1591    1923    2241 
   Size:       1072     519     332     318

  Fragments arranged by size: 

                1072     519     332     318
 
StuI AGG'CCT

Cuts at:      0     376    2241 
   Size:        376    1865
 
StyI C'CwwG_G

Cuts at:      0    1289    1295    1496    2241 
   Size:       1289       6     201     745

  Fragments arranged by size: 

                1289     745     201       6
 
TatI w'GTAC_w

Cuts at:      0    2180    2241 
   Size:       2180      61
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1125    1157    2241 
   Size:       1125      32    1084

  Fragments arranged by size: 

                1125    1084      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     897    1395    2241 
   Size:        897     498     846

  Fragments arranged by size: 

                 897     846     498
 
ZraI GAC'GTC

Cuts at:      0     258    2241 
   Size:        258    1983
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI   AflIII     AlfI     AloI    AlwNI
     ApaI    ApaLI     ApoI     AvaI     BanI    BanII     BbsI    BbvCI
     BcgI     BglI    BglII Bme1580I    BmgBI     BmrI     BmtI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI
    BsrDI    BsrFI    BsrGI   BstAPI    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     DraI   DraIII     EaeI     EarI     EciI  Eco57MI
 EcoO109I    EcoRI     FalI     FseI     FspI    HaeII    Hin4I   HincII
     MmeI     MscI   MspA1I     NaeI   NgoMIV     NheI     NspI     PasI
     PciI    PflMI     PfoI     PmlI    PpuMI   PspOMI     PstI    PvuII
    SacII     SalI     SfcI     SfiI     SmlI     StuI     StyI     TatI
     TstI     XcmI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AleI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI    BciVI     BclI
    BfrBI     BlpI     BplI    BsaBI    BsiWI     BsmI    BsmBI    BsrBI
   BssHII    BssSI    BstBI   BstEII  BstZ17I     ClaI    CspCI     DrdI
     EagI  EcoICRI    EcoNI    EcoRV    FspAI  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MslI     NarI     NcoI     NdeI     NotI
     NruI     NsiI     PacI     PmeI     PpiI    PshAI     PsiI    PspXI
     PsrI     PvuI    RsrII     SacI    SanDI     SapI     SbfI     ScaI
    SexAI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI    TaqII  Tth111I     XbaI     XhoI     XmaI
     XmnI
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