Restriction map for pF1KB4051
##################################
# NO CUT ENZYMES (  171 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflIII      AgeI        
AhdI        Alw44I      Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BclI        BcnI        BglI        BlnI        
BpmI        BpuAI       BsaAI       BsaHI       BsaI        
BsaWI       BseAI       BsiEI       BsiHKAI     BsiWI       
BsmBI       BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstZ17I     
Cfr10I      Cfr13I      ClaI        CpoI        DraII       
DraIII      DrdI        EaeI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT14I     FauI        
FbaI        FseI        FspI        HapII       HgaI        
Hin1I       HincII      HindII      HindIII     HpaI        
HpaII       KasI        KpnI        Ksp632I     KspI        
MaeII       MfeI        MluI        MluNI       MroI        
MscI        MspI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
Sau96I      SbfI        ScaI        SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        Tth111I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AcsI          113  AAATTC               [GA]^AATT[CT]
AflII         493  CTTAAG               C^TTAAG
AlwNI         593  CAGCCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          946  CCTCAGG              CC^T[ACGT]AGG
ApoI          113  AAATTC               [GA]^AATT[CT]
BciVI         531  GGATAC               GTATCC(6/5)
BfrI          493  CTTAAG               C^TTAAG
BglII         489  AGATCT               A^GATCT
BlpI          441  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      441  GCTGAGC              GC^T[ACGT]AGC
BsaBI         732  GATTATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI         371  GAGGAG               GAGGAG(10/8)
BsgI           92  CTGCAC               GTGCAG(16/14)
BsmAI         778  GAGAC                GTCTC(1/5)
BsmFI         252  GTCCC                GGGAC(10/14)
BsmI          214  GAATGC               GAATGC(1/-1)
BsrDI         745  GCAATG               GCAATG(2/0)
BsrI          536  CCAGT                ACTGG(1/-1)
BstXI         536  CCAGTGACTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         489  AGATCT               [GA]^GATC[CT]
Bsu36I        946  CCTCAGG              CC^T[ACGT]AGG
CelII         441  GCTGAGC              GC^T[ACGT]AGC
CfoI          332  GCGC                 GCG^C
DraI         1000  TTTAAA               TTT^AAA
DsaI          255  CCACGG               C^C[GA][CT]GG
Eco81I        946  CCTCAGG              CC^T[ACGT]AGG
EcoNI         222  CCTACAAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I       216  ATGCAT               ATGCA^T
HaeII         331  AGCGCC               [GA]GCGC^[CT]
HaeIII         43  GGCC                 GG^CC
HhaI          332  GCGC                 GCG^C
HinP1I        332  GCGC                 G^CGC
HinfI         201  GATTC                G^A[ACGT]TC
HphI            3  GGTGA                GGTGA(8/7)
MamI          732  GATTATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          489  AGATCT               [GA]^GATC[CT]
MwoI           94  GCACGGGTGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NsiI          216  ATGCAT               ATGCA^T
NspI          652  GCATGT               [GA]CATG^[CT]
SexAI         523  ACCAGGT              A^CC[AT]GGT
SmlI          493  CTTAAG               C^T[CT][GA]AG
TaqI          117  TCGA                 T^CGA
TfiI          201  GATTC                G^A[AT]TC
TthHB8I       117  TCGA                 T^CGA
XhoII         489  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AfaI           21  GTAC                 GT^AC
              988  GTAC                
BbvI         1010  GCAGC                GCAGC(8/12)
              937  GCTGC               
BfaI          145  CTAG                 C^TAG
              973  CTAG                
BmyI          164  GGGCAC               G[AGT]GC[ACT]^C
              449  GTGCCC              
Bsp1286I      164  GGGCAC               G[AGT]GC[ACT]^C
              449  GTGCCC              
Cac8I          11  GCCAGC               GC[ACGT]^[ACGT]GC
              933  GCTGGC              
Fnu4HI        937  GCTGC                GC^[ACGT]GC
             1010  GCAGC               
ItaI          937  GCTGC                GC^[ACGT]GC
             1010  GCAGC               
MaeI          145  CTAG                 C^TAG
              973  CTAG                
MslI          190  CATCCAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              876  CATTCCTGTG          
MspA1I        439  CAGCTG               C[AC]G^C[GT]G
             1011  CAGCTG              
NlaIV         546  GGAACC               GG[ACGT]^[ACGT]CC
              675  GGCTCC              
PvuII         439  CAGCTG               CAG^CTG
             1011  CAGCTG              
RsaI           21  GTAC                 GT^AC
              988  GTAC                
SfaNI         961  GATGC                GCATC(5/9)
              218  GCATC               
TseI          937  GCTGC                G^C[AT]GC
             1010  GCAGC               


#########################################
# 3 OR MORE CUT ENZYMES (   26 enzymes) #
#########################################

AluI        AlwI        BsaJI       BsiYI       BslI        
BssKI       BstF5I      BstNI       DdeI        DpnII       
EcoRII      FokI        MaeIII      MboI        MboII       
MnlI        MseI        MvaI        NdeII       NlaIII      
Sau3AI      ScrFI       Tru9I       Tsp45I      Tsp509I     
TspRI       
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